DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/16nov30a/protomo_alignments/tiltseries0001/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/16nov30a/protomo_alignments/tiltseries0001/defocus_estimation/series0001_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/16nov30a/protomo_alignments/tiltseries0001/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.7, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.76 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.1 dFMin[A], dFMax[A], FStep: 40000.0, 60000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 48.0 40000.0 55000.0 43500.0 1 46.0 42500.0 40000.0 44500.0 2 44.0 58500.0 41500.0 44000.0 3 42.0 40000.0 45000.0 47500.0 4 40.0 57000.0 52000.0 60000.0 5 38.0 40000.0 40500.0 59000.0 6 36.0 40000.0 42000.0 41000.0 7 34.0 41000.0 52500.0 43000.0 8 32.0 41000.0 47000.0 43000.0 9 30.0 45500.0 41000.0 40500.0 10 28.0 44000.0 40000.0 41500.0 11 26.0 49000.0 40000.0 44000.0 12 24.0 40000.0 42000.0 40000.0 13 22.0 41000.0 40000.0 43500.0 14 20.0 47000.0 40000.0 40000.0 15 18.0 50000.0 57000.0 40500.0 16 16.0 53500.0 47500.0 44000.0 17 14.0 44500.0 40500.0 47500.0 18 12.0 40500.0 58000.0 48500.0 19 10.0 47500.0 40000.0 40500.0 20 8.0 47000.0 44000.0 40000.0 21 6.0 40000.0 48500.0 42000.0 22 4.0 41500.0 46000.0 40500.0 23 2.0 43500.0 53500.0 40500.0 24 0.0 42500.0 40000.0 40000.0 25 -0.0 40500.0 40000.0 46000.0 26 -2.0 40500.0 40000.0 40000.0 27 -4.0 40000.0 40000.0 40000.0 28 -6.0 44000.0 40000.0 54500.0 29 -8.0 40500.0 46000.0 41000.0 30 -10.0 43000.0 40000.0 40000.0 31 -12.0 42500.0 43000.0 40000.0 32 -14.0 40000.0 49000.0 44000.0 33 -16.0 44000.0 42500.0 48000.0 34 -18.0 43500.0 40000.0 48500.0 35 -20.0 40500.0 46000.0 42000.0 36 -22.0 47500.0 40000.0 42000.0 37 -24.0 40000.0 43000.0 49500.0 38 -26.0 40500.0 49500.0 46000.0 39 -28.0 51500.0 48000.0 46000.0 40 -30.0 42000.0 41000.0 59000.0 41 -32.0 46000.0 40000.0 43000.0 42 -34.0 44000.0 41500.0 60000.0 43 -36.0 55500.0 47000.0 53500.0 44 -38.0 44500.0 50500.0 40000.0 45 -40.0 41000.0 46500.0 50000.0 46 -42.0 58500.0 45500.0 42000.0 47 -44.0 40000.0 43000.0 42000.0 48 -46.0 55500.0 46500.0 55000.0 49 -48.0 47500.0 41000.0 52500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)