protomo2aligner.py --my_tlt=False --r5_kernel_x=5 --r5_iters=0 --r4_mask_apod_y=10 --r4_mask_apod_x=10 --corr_mode=mcf --rundir=/gpfs/appion/anoble/18mar15h/protomo_alignments/tiltseries0003_thick50 --r1_peak_search_radius_y=300.0 --r1_peak_search_radius_x=300.0 --serialem_stack= --r3_highpass_diameter_x=3137.0 --r4_lowpass_diameter_x=5.32 --filename_prefix= --r4_cmdiameter_x=100.0 --r4_cmdiameter_y=100.0 --r1_region_x=2417 --r1_region_y=2357 --cmdiameter_y=100.0 --r2_corr_mode=mcf --r5_highpass_diameter_x=3137.0 --r4_lowpass_apod_y=13.0 --r4_lowpass_apod_x=13.0 --r3_kernel_y=5 --r3_kernel_x=5 --r3_lowpass_apod_x=27.0 --r3_lowpass_apod_y=27.0 --manual_alignment_finished=False --r5_body=0 --runname=tiltseries0003_thick50 --r2_region_y=2357 --r2_region_x=2417 --r2_sampling=4 --video_type=html5vid --rotation=true --r2_highpass_apod_y=1569.0 --defocus_ang_positive=90 --r5_mask_width_x=2392 --frame_aligned=True --image_file_type=mrc --r3_highpass_diameter_y=3137.0 --r3_highpass_apod_x=1569.0 --r5_cmdiameter_y=100.0 --r5_cmdiameter_x=100.0 --r2_mask_width_x=579 --r2_mask_width_y=564 --r5_region_x=2417 --r5_region_y=2357 --highpass_diameter_y=0.001 --highpass_diameter_x=0.001 --create_reconstruction=true --r5_mask_apod_x=10 --r5_mask_apod_y=10 --r4_lowpass_diameter_y=5.32 --r1_body=0 --r1_mask_apod_x=10 --defocus_difference=0.2 --correlation_size_y=160 --correlation_size_x=160 --r2_cmdiameter_x=100.0 --r2_cmdiameter_y=100.0 --gridsearch_limit=2.0 --azimuth_stability_check=False --r1_highpass_diameter_y=3137.0 --r4_kernel_x=5 --r4_kernel_y=5 --amp_contrast_defocus=0.07 --peak_search_radius_x=100.0 --peak_search_radius_y=100.0 --r5_mask_width_y=2332 --window_area=0.95 --r1_corr_mode=mcf --gradient=true --create_tilt_video=true --mask_apod_y=10 --r5_peak_search_radius_y=300.0 --exclude_images_by_angle= --r3_mask_apod_x=10 --r3_mask_apod_y=10 --norotations=false --r3_highpass_apod_y=1569.0 --r3_iters=5 --r1_highpass_diameter_x=3137.0 --r2_lowpass_diameter_y=21.28 --map_size_y=479 --map_size_z=200 --r5_lowpass_diameter_y=2.66 --r5_lowpass_diameter_x=2.66 --max_shift=999999 --cachedir=cache --clip_high=3.5 --grow=0 --r3_cmdiameter_y=100.0 --r3_cmdiameter_x=100.0 --keep_recons=false --r4_mask_width_x=2392 --r4_mask_width_y=2332 --r5_highpass_apod_y=1569.0 --logging=true --DefocusTol=200 --filter=median --sessionname=18mar15h --exclude_images=999999 --commit=False --r1_kernel_y=5 --r1_kernel_x=5 --dose_presets=False --mask_apod_x=10 --r4_corr_mode=mcf --show_window_size=true --defocus_estimate=False --r4_iters=10 --amp_contrast_ctf=0.07 --r1_highpass_apod_y=1569.0 --r1_highpass_apod_x=1569.0 --r2_highpass_apod_x=1569.0 --sampling=8 --starting_tlt_file=Coarse_Iter_1 --r5_lowpass_apod_x=7.0 --r5_lowpass_apod_y=7.0 --dose_c=2.81 --r1_cmdiameter_y=100.0 --further_alignment=False --r1_lowpass_diameter_y=42.56 --r1_lowpass_diameter_x=42.56 --r4_sampling=1 --res_max=10 --r1_mask_apod_y=10 --res_min=10000 --scale_switch=31 --dose_b=-1.665 --positive=90.0 --r3_peak_search_radius_y=300.0 --r3_peak_search_radius_x=300.0 --negative=-90.0 --parallel=True --tiltseries=3 --r5_corr_mode=pcf --change_refimg=False --r3_mask_width_y=1153 --r3_mask_width_x=1183 --map_sampling=8 --thr_low=0 --gridsearch_step=0.1 --coarse=False --r2_iters=10 --region_y=512 --r4_highpass_diameter_y=3137.0 --r4_highpass_diameter_x=3137.0 --mask_width_x=1024 --highpass_apod_x=0.002 --binning=true --r1_lowpass_apod_y=106.0 --thr_high=999999 --r5_sampling=1 --highpass_apod_y=0.002 --defocus_min=0 --r1_sampling=8 --ctf_correct=False --r2_highpass_diameter_y=3137.0 --r2_mask_apod_y=10 --r2_mask_apod_x=10 --desired_ref_tilt_angle=0.0 --r5_highpass_apod_x=1569.0 --defocus_save=0 --protomo_outdir=out --r5_highpass_diameter_y=3137.0 --defocus_max=0 --r4_region_y=2357 --r4_region_x=2417 --r5_kernel_y=5 --r3_region_x=2417 --r3_region_y=2357 --r4_body=0 --serialem_mdoc= --r2_peak_search_radius_x=300.0 --r2_peak_search_radius_y=300.0 --thickness=50 --expid=6300 --r2_body=0 --orientation=true --do_estimation=false --iter_gradient=false --projectid=506 --map_size_x=463 --r3_body=0 --r3_lowpass_diameter_y=10.64 --r3_lowpass_diameter_x=10.64 --mask_width_y=1024 --max_correction=999999 --elevation=false --r2_kernel_x=5 --r2_kernel_y=5 --slab=true --pixelsize=1.331 --link=False --r2_highpass_diameter_x=3137.0 --lowpass_diameter_x=0.5 --lowpass_diameter_y=0.5 --defocus=3 --border=100 --r3_corr_mode=mcf --r2_lowpass_apod_y=53.0 --exclude_images_by_angle_accuracy=0.05 --r2_lowpass_apod_x=53.0 --defocus_ang_negative=-90 --r3_sampling=2 --description= --make_searchable=True --translimit=377.0 --iters=1 --r5_peak_search_radius_x=300.0 --r1_iters=10 --r1_cmdiameter_x=100.0 --preprocessing=true --scale=false --iWidth=20 --r2_lowpass_diameter_x=21.28 --dimx=3710 --dimy=3838 --r4_peak_search_radius_x=300.0 --r4_peak_search_radius_y=300.0 --r1_lowpass_apod_x=106.0 --region_x=512 --manual_alignment=False --loglevel=2 --restart_cycle=0 --dose_a=0.245 --lowpass_apod_y=0.05 --lowpass_apod_x=0.05 --kernel_x=3 --kernel_y=3 --clip_low=3.5 --azimuth_max_deviation=3.0 --tilt_clip=true --r4_highpass_apod_x=1569.0 --r4_highpass_apod_y=1569.0 --azimuth=true --r1_mask_width_y=269 --r1_mask_width_x=277 --jobtype=protomo --from_multirefine=True node39 Start time: 2018yr03m15d-11hr52m04s Description: Using alignment .tlt file as determined by protomo2multirefine.py.Starting Protomo alignment Beginning Refinements Beginning Refinement Iteration #1, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) lowpass = 42.56 Angstroms Round #1. Parameters in Protomo units: (Note: At binned by 8, pixelsize is 10.648 Angstroms, Nyquist is 21.296 Angstroms) window.lowpass.apodization = { 0.200905660377 0.200905660377 } align.translimit = 47.125e0 map.sampling = 8 window.highpass.diameter = { 0.00678865157794 0.00678865157794 } window.highpass.apodization = { 0.0135729764181 0.0135729764181 } window.mask.apodization = { 10 10 } align.mask.width = { 277 269 } sampling = 8 align.mask.apodization = { 10 10 } map.body = 6.64086328748 map.lowpass.diameter = { 0.50037593985 0.50037593985 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 302 294 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 277 269 } reference.body = 6.64086328748 window.lowpass.diameter = { 0.50037593985 0.50037593985 } align.correlation.mode = mcf No parameters were switched for Iteration #1 Creating quality assessment statistics... CCMS(shift) = 0.05666 for Iteration #1 of Tilt-Series #3. CCMS(rotations) = 0.95388 for Iteration #1 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #1 of Tilt-Series #3. The scaled sum of CCMS values is #0.04741 for iteration 1 of Tilt-Series #3. Alignment quality for Iteration #1 of Tilt-Series #3 (18mar15h): Bad - 1st iteration is always bad because the geometry has not yet been estimated (confidence: 100.000000%)Creating Depiction Videos for Iteration #1 in Parallel... Creating Refinement tilt-series video for iteration #1... Generating Refinement reconstruction for iteration #1... No Round parameters changed for Iteration #2 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #2, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #2 Creating quality assessment statistics... CCMS(shift) = 0.04087 for Iteration #2 of Tilt-Series #3. CCMS(rotations) = 0.74572 for Iteration #2 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #2 of Tilt-Series #3. The scaled sum of CCMS values is #0.03535 for iteration 2 of Tilt-Series #3. Alignment quality for Iteration #2 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Creating Depiction Videos for Iteration #2 in Parallel... Creating Refinement tilt-series video for iteration #2... Generating Refinement reconstruction for iteration #2... No Round parameters changed for Iteration #3 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #3, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #3 Creating quality assessment statistics... CCMS(shift) = 0.01533 for Iteration #3 of Tilt-Series #3. CCMS(rotations) = 0.29348 for Iteration #3 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #3 of Tilt-Series #3. The scaled sum of CCMS values is #0.01353 for iteration 3 of Tilt-Series #3. Alignment quality for Iteration #3 of Tilt-Series #3 (18mar15h): Good (confidence: 100.000000%)Creating Depiction Videos for Iteration #3 in Parallel... Creating Refinement tilt-series video for iteration #3... Generating Refinement reconstruction for iteration #3... No Round parameters changed for Iteration #4 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #4, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #4 Creating quality assessment statistics... CCMS(shift) = 0.00952 for Iteration #4 of Tilt-Series #3. CCMS(rotations) = 0.29768 for Iteration #4 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #4 of Tilt-Series #3. The scaled sum of CCMS values is #0.01071 for iteration 4 of Tilt-Series #3. Alignment quality for Iteration #4 of Tilt-Series #3 (18mar15h): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #4 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #4 in Parallel... Creating Refinement tilt-series video for iteration #4... Generating Refinement reconstruction for iteration #4... No Round parameters changed for Iteration #5 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #5, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #5 Creating quality assessment statistics... CCMS(shift) = 0.00937 for Iteration #5 of Tilt-Series #3. CCMS(rotations) = 0.34513 for Iteration #5 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #5 of Tilt-Series #3. The scaled sum of CCMS values is #0.01159 for iteration 5 of Tilt-Series #3. Alignment quality for Iteration #5 of Tilt-Series #3 (18mar15h): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #5 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #5 in Parallel... Creating Refinement tilt-series video for iteration #5... Generating Refinement reconstruction for iteration #5... No Round parameters changed for Iteration #6 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #6, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #6 Creating quality assessment statistics... CCMS(shift) = 0.00949 for Iteration #6 of Tilt-Series #3. CCMS(rotations) = 0.30771 for Iteration #6 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #6 of Tilt-Series #3. The scaled sum of CCMS values is #0.0109 for iteration 6 of Tilt-Series #3. Alignment quality for Iteration #6 of Tilt-Series #3 (18mar15h): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #6 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #6 in Parallel... Creating Refinement tilt-series video for iteration #6... Generating Refinement reconstruction for iteration #6... No Round parameters changed for Iteration #7 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #7, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #7 Creating quality assessment statistics... CCMS(shift) = 0.00949 for Iteration #7 of Tilt-Series #3. CCMS(rotations) = 0.344 for Iteration #7 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #7 of Tilt-Series #3. The scaled sum of CCMS values is #0.01163 for iteration 7 of Tilt-Series #3. Alignment quality for Iteration #7 of Tilt-Series #3 (18mar15h): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #7 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #7 in Parallel... Creating Refinement tilt-series video for iteration #7... Generating Refinement reconstruction for iteration #7... No Round parameters changed for Iteration #8 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #8, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #8 Creating quality assessment statistics... CCMS(shift) = 0.00896 for Iteration #8 of Tilt-Series #3. CCMS(rotations) = 0.30463 for Iteration #8 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #8 of Tilt-Series #3. The scaled sum of CCMS values is #0.01057 for iteration 8 of Tilt-Series #3. Alignment quality for Iteration #8 of Tilt-Series #3 (18mar15h): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #8 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #8 in Parallel... Creating Refinement tilt-series video for iteration #8... Generating Refinement reconstruction for iteration #8... No Round parameters changed for Iteration #9 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #9, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #9 Creating quality assessment statistics... CCMS(shift) = 0.00892 for Iteration #9 of Tilt-Series #3. CCMS(rotations) = 0.33042 for Iteration #9 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #9 of Tilt-Series #3. The scaled sum of CCMS values is #0.01107 for iteration 9 of Tilt-Series #3. Alignment quality for Iteration #9 of Tilt-Series #3 (18mar15h): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #9 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #9 in Parallel... Creating Refinement tilt-series video for iteration #9... Generating Refinement reconstruction for iteration #9... No Round parameters changed for Iteration #10 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #10, Round #1 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #10 Creating quality assessment statistics... CCMS(shift) = 0.00887 for Iteration #10 of Tilt-Series #3. CCMS(rotations) = 0.29751 for Iteration #10 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #10 of Tilt-Series #3. The scaled sum of CCMS values is #0.01039 for iteration 10 of Tilt-Series #3. Alignment quality for Iteration #10 of Tilt-Series #3 (18mar15h): Very Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #10 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #10 in Parallel... Creating Refinement tilt-series video for iteration #10... Generating Refinement reconstruction for iteration #10... Beginning Refinement Iteration #11, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) lowpass = 21.28 angstroms Round #2. Parameters in Protomo units: (Note: At binned by 4, pixelsize is 5.324 Angstroms, Nyquist is 10.648 Angstroms) window.lowpass.apodization = { 0.200905660377 0.200905660377 } align.translimit = 94.25e0 map.sampling = 4 window.highpass.diameter = { 0.00339432578897 0.00339432578897 } window.highpass.apodization = { 0.00678648820905 0.00678648820905 } window.mask.apodization = { 10 10 } align.mask.width = { 579 564 } sampling = 4 align.mask.apodization = { 10 10 } map.body = 13.281726575 map.lowpass.diameter = { 0.50037593985 0.50037593985 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 604 589 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 579 564 } reference.body = 13.281726575 window.lowpass.diameter = { 0.50037593985 0.50037593985 } align.correlation.mode = mcf No parameters were switched for Iteration #11 Creating quality assessment statistics... CCMS(shift) = 0.01955 for Iteration #11 of Tilt-Series #3. CCMS(rotations) = 0.2789 for Iteration #11 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #11 of Tilt-Series #3. The scaled sum of CCMS values is #0.01535 for iteration 11 of Tilt-Series #3. Alignment quality for Iteration #11 of Tilt-Series #3 (18mar15h): Good (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #11 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #11 in Parallel... Creating Refinement tilt-series video for iteration #11... Generating Refinement reconstruction for iteration #11... No Round parameters changed for Iteration #12 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #12, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #12 Creating quality assessment statistics... CCMS(shift) = 0.01973 for Iteration #12 of Tilt-Series #3. CCMS(rotations) = 0.56629 for Iteration #12 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #12 of Tilt-Series #3. The scaled sum of CCMS values is #0.02119 for iteration 12 of Tilt-Series #3. Alignment quality for Iteration #12 of Tilt-Series #3 (18mar15h): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #12 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #12 in Parallel... Creating Refinement tilt-series video for iteration #12... Generating Refinement reconstruction for iteration #12... No Round parameters changed for Iteration #13 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #13, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #13 Creating quality assessment statistics... CCMS(shift) = 0.02255 for Iteration #13 of Tilt-Series #3. CCMS(rotations) = 0.85054 for Iteration #13 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #13 of Tilt-Series #3. The scaled sum of CCMS values is #0.02829 for iteration 13 of Tilt-Series #3. Alignment quality for Iteration #13 of Tilt-Series #3 (18mar15h): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #13 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #13 in Parallel... Creating Refinement tilt-series video for iteration #13... Generating Refinement reconstruction for iteration #13... No Round parameters changed for Iteration #14 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #14, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #14 Creating quality assessment statistics... CCMS(shift) = 0.01461 for Iteration #14 of Tilt-Series #3. CCMS(rotations) = 1.07274 for Iteration #14 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #14 of Tilt-Series #3. The scaled sum of CCMS values is #0.02876 for iteration 14 of Tilt-Series #3. Alignment quality for Iteration #14 of Tilt-Series #3 (18mar15h): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #14 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #14 in Parallel... Creating Refinement tilt-series video for iteration #14... Generating Refinement reconstruction for iteration #14... No Round parameters changed for Iteration #15 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #15, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #15 Creating quality assessment statistics... CCMS(shift) = 0.02437 for Iteration #15 of Tilt-Series #3. CCMS(rotations) = 0.81126 for Iteration #15 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #15 of Tilt-Series #3. The scaled sum of CCMS values is #0.02841 for iteration 15 of Tilt-Series #3. Alignment quality for Iteration #15 of Tilt-Series #3 (18mar15h): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #15 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #15 in Parallel... Creating Refinement tilt-series video for iteration #15... Generating Refinement reconstruction for iteration #15... No Round parameters changed for Iteration #16 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #16, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #16 Creating quality assessment statistics... CCMS(shift) = 0.0189 for Iteration #16 of Tilt-Series #3. CCMS(rotations) = 1.07757 for Iteration #16 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #16 of Tilt-Series #3. The scaled sum of CCMS values is #0.031 for iteration 16 of Tilt-Series #3. Alignment quality for Iteration #16 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #16 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #16 in Parallel... Creating Refinement tilt-series video for iteration #16... Generating Refinement reconstruction for iteration #16... No Round parameters changed for Iteration #17 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #17, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #17 Creating quality assessment statistics... CCMS(shift) = 0.01549 for Iteration #17 of Tilt-Series #3. CCMS(rotations) = 1.17349 for Iteration #17 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #17 of Tilt-Series #3. The scaled sum of CCMS values is #0.03122 for iteration 17 of Tilt-Series #3. Alignment quality for Iteration #17 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #17 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #17 in Parallel... Creating Refinement tilt-series video for iteration #17... Generating Refinement reconstruction for iteration #17... No Round parameters changed for Iteration #18 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #18, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #18 Creating quality assessment statistics... CCMS(shift) = 0.02135 for Iteration #18 of Tilt-Series #3. CCMS(rotations) = 0.85833 for Iteration #18 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #18 of Tilt-Series #3. The scaled sum of CCMS values is #0.02784 for iteration 18 of Tilt-Series #3. Alignment quality for Iteration #18 of Tilt-Series #3 (18mar15h): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #18 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #18 in Parallel... Creating Refinement tilt-series video for iteration #18... Generating Refinement reconstruction for iteration #18... No Round parameters changed for Iteration #19 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #19, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #19 Creating quality assessment statistics... CCMS(shift) = 0.01428 for Iteration #19 of Tilt-Series #3. CCMS(rotations) = 1.04524 for Iteration #19 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #19 of Tilt-Series #3. The scaled sum of CCMS values is #0.02804 for iteration 19 of Tilt-Series #3. Alignment quality for Iteration #19 of Tilt-Series #3 (18mar15h): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #19 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #19 in Parallel... Creating Refinement tilt-series video for iteration #19... Generating Refinement reconstruction for iteration #19... No Round parameters changed for Iteration #20 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #20, Round #2 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #20 Creating quality assessment statistics... CCMS(shift) = 0.02075 for Iteration #20 of Tilt-Series #3. CCMS(rotations) = 0.73912 for Iteration #20 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #20 of Tilt-Series #3. The scaled sum of CCMS values is #0.02515 for iteration 20 of Tilt-Series #3. Alignment quality for Iteration #20 of Tilt-Series #3 (18mar15h): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #20 of Tilt-Series #3 (18mar15h): StableCreating Depiction Videos for Iteration #20 in Parallel... Creating Refinement tilt-series video for iteration #20... Generating Refinement reconstruction for iteration #20... Beginning Refinement Iteration #21, Round #3 of Tilt-Series #3 (tiltseries0003_thick50) lowpass = 10.64 angstroms Round #3. Parameters in Protomo units: (Note: At binned by 2, pixelsize is 2.662 Angstroms, Nyquist is 5.324 Angstroms) window.lowpass.apodization = { 0.197185185185 0.197185185185 } align.translimit = 188.5e0 map.sampling = 2 window.highpass.diameter = { 0.00169716289449 0.00169716289449 } window.highpass.apodization = { 0.00339324410453 0.00339324410453 } window.mask.apodization = { 10 10 } align.mask.width = { 1183 1153 } sampling = 2 align.mask.apodization = { 10 10 } map.body = 26.5634531499 map.lowpass.diameter = { 0.50037593985 0.50037593985 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 1208 1178 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 1183 1153 } reference.body = 26.5634531499 window.lowpass.diameter = { 0.50037593985 0.50037593985 } align.correlation.mode = mcf No parameters were switched for Iteration #21 Creating quality assessment statistics... CCMS(shift) = 0.02317 for Iteration #21 of Tilt-Series #3. CCMS(rotations) = 0.69513 for Iteration #21 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #21 of Tilt-Series #3. The scaled sum of CCMS values is #0.02549 for iteration 21 of Tilt-Series #3. Alignment quality for Iteration #21 of Tilt-Series #3 (18mar15h): Okay (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #21 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #21 in Parallel... Creating Refinement tilt-series video for iteration #21... Generating Refinement reconstruction for iteration #21... No Round parameters changed for Iteration #22 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #22, Round #3 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #22 Creating quality assessment statistics... CCMS(shift) = 0.01881 for Iteration #22 of Tilt-Series #3. CCMS(rotations) = 1.07345 for Iteration #22 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #22 of Tilt-Series #3. The scaled sum of CCMS values is #0.03087 for iteration 22 of Tilt-Series #3. Alignment quality for Iteration #22 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #22 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #22 in Parallel... Creating Refinement tilt-series video for iteration #22... Generating Refinement reconstruction for iteration #22... No Round parameters changed for Iteration #23 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #23, Round #3 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #23 Creating quality assessment statistics... CCMS(shift) = 0.02015 for Iteration #23 of Tilt-Series #3. CCMS(rotations) = 1.24169 for Iteration #23 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #23 of Tilt-Series #3. The scaled sum of CCMS values is #0.03491 for iteration 23 of Tilt-Series #3. Alignment quality for Iteration #23 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #23 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #23 in Parallel... Creating Refinement tilt-series video for iteration #23... Generating Refinement reconstruction for iteration #23... No Round parameters changed for Iteration #24 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #24, Round #3 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #24 Creating quality assessment statistics... CCMS(shift) = 0.02136 for Iteration #24 of Tilt-Series #3. CCMS(rotations) = 1.2949 for Iteration #24 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #24 of Tilt-Series #3. The scaled sum of CCMS values is #0.03658 for iteration 24 of Tilt-Series #3. Alignment quality for Iteration #24 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #24 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #24 in Parallel... Creating Refinement tilt-series video for iteration #24... Generating Refinement reconstruction for iteration #24... No Round parameters changed for Iteration #25 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #25, Round #3 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #25 Creating quality assessment statistics... CCMS(shift) = 0.02338 for Iteration #25 of Tilt-Series #3. CCMS(rotations) = 1.19285 for Iteration #25 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #25 of Tilt-Series #3. The scaled sum of CCMS values is #0.03555 for iteration 25 of Tilt-Series #3. Alignment quality for Iteration #25 of Tilt-Series #3 (18mar15h): Bad (confidence: 96.774194%)Tilt model stability (with respect to the last 3 iterations) at Iteration #25 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #25 in Parallel... Creating Refinement tilt-series video for iteration #25... Generating Refinement reconstruction for iteration #25... Beginning Refinement Iteration #26, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) lowpass = 5.32 angstroms Round #4. Parameters in Protomo units: (Note: At binned by 1, pixelsize is 1.331 Angstroms, Nyquist is 2.662 Angstroms) window.lowpass.apodization = { 0.204769230769 0.204769230769 } align.translimit = 377.0e0 map.sampling = 1 window.highpass.diameter = { 0.000848581447243 0.000848581447243 } window.highpass.apodization = { 0.00169662205226 0.00169662205226 } window.mask.apodization = { 10 10 } align.mask.width = { 2392 2332 } sampling = 1 align.mask.apodization = { 10 10 } map.body = 53.1269062999 map.lowpass.diameter = { 0.50037593985 0.50037593985 } align.peaksearch.radius = { 300.0 300.0 } window.size = { 2417 2357 } preprocess.mask.kernel = { 5 5 } window.mask.width = { 2392 2332 } reference.body = 53.1269062999 window.lowpass.diameter = { 0.50037593985 0.50037593985 } align.correlation.mode = mcf No parameters were switched for Iteration #26 Creating quality assessment statistics... CCMS(shift) = 0.03063 for Iteration #26 of Tilt-Series #3. CCMS(rotations) = 1.38947 for Iteration #26 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #26 of Tilt-Series #3. The scaled sum of CCMS values is #0.0431 for iteration 26 of Tilt-Series #3. Alignment quality for Iteration #26 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #26 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #26 in Parallel... Creating Refinement tilt-series video for iteration #26... Generating Refinement reconstruction for iteration #26... No Round parameters changed for Iteration #27 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #27, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #27 Creating quality assessment statistics... CCMS(shift) = 0.02227 for Iteration #27 of Tilt-Series #3. CCMS(rotations) = 1.74738 for Iteration #27 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #27 of Tilt-Series #3. The scaled sum of CCMS values is #0.04608 for iteration 27 of Tilt-Series #3. Alignment quality for Iteration #27 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #27 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #27 in Parallel... Creating Refinement tilt-series video for iteration #27... Generating Refinement reconstruction for iteration #27... No Round parameters changed for Iteration #28 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #28, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #28 Creating quality assessment statistics... CCMS(shift) = 0.02202 for Iteration #28 of Tilt-Series #3. CCMS(rotations) = 1.71787 for Iteration #28 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #28 of Tilt-Series #3. The scaled sum of CCMS values is #0.04537 for iteration 28 of Tilt-Series #3. Alignment quality for Iteration #28 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #28 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #28 in Parallel... Creating Refinement tilt-series video for iteration #28... Generating Refinement reconstruction for iteration #28... No Round parameters changed for Iteration #29 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #29, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #29 Creating quality assessment statistics... CCMS(shift) = 0.02757 for Iteration #29 of Tilt-Series #3. CCMS(rotations) = 1.89995 for Iteration #29 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #29 of Tilt-Series #3. The scaled sum of CCMS values is #0.05178 for iteration 29 of Tilt-Series #3. Alignment quality for Iteration #29 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #29 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #29 in Parallel... Creating Refinement tilt-series video for iteration #29... Generating Refinement reconstruction for iteration #29... No Round parameters changed for Iteration #30 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #30, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #30 Creating quality assessment statistics... CCMS(shift) = 0.02425 for Iteration #30 of Tilt-Series #3. CCMS(rotations) = 1.86471 for Iteration #30 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #30 of Tilt-Series #3. The scaled sum of CCMS values is #0.04942 for iteration 30 of Tilt-Series #3. Alignment quality for Iteration #30 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #30 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #30 in Parallel... Creating Refinement tilt-series video for iteration #30... Generating Refinement reconstruction for iteration #30... No Round parameters changed for Iteration #31 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #31, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) fit.scale switched from false to true on Iteration #31Creating quality assessment statistics... CCMS(shift) = 0.02218 for Iteration #31 of Tilt-Series #3. CCMS(rotations) = 1.94177 for Iteration #31 of Tilt-Series #3. CCMS(scale) = 0.0 for Iteration #31 of Tilt-Series #3. The scaled sum of CCMS values is #0.04992 for iteration 31 of Tilt-Series #3. Alignment quality for Iteration #31 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #31 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #31 in Parallel... Creating Refinement tilt-series video for iteration #31... Generating Refinement reconstruction for iteration #31... No Round parameters changed for Iteration #32 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #32, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #32 Creating quality assessment statistics... CCMS(shift) = 0.02219 for Iteration #32 of Tilt-Series #3. CCMS(rotations) = 1.9796 for Iteration #32 of Tilt-Series #3. CCMS(scale) = 0.0036 for Iteration #32 of Tilt-Series #3. The scaled sum of CCMS values is #0.03499 for iteration 32 of Tilt-Series #3. Alignment quality for Iteration #32 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #32 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #32 in Parallel... Creating Refinement tilt-series video for iteration #32... Generating Refinement reconstruction for iteration #32... No Round parameters changed for Iteration #33 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #33, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #33 Creating quality assessment statistics... CCMS(shift) = 0.02907 for Iteration #33 of Tilt-Series #3. CCMS(rotations) = 1.8913 for Iteration #33 of Tilt-Series #3. CCMS(scale) = 0.00364 for Iteration #33 of Tilt-Series #3. The scaled sum of CCMS values is #0.03612 for iteration 33 of Tilt-Series #3. Alignment quality for Iteration #33 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #33 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #33 in Parallel... Creating Refinement tilt-series video for iteration #33... Generating Refinement reconstruction for iteration #33... No Round parameters changed for Iteration #34 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #34, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #34 Creating quality assessment statistics... CCMS(shift) = 0.02539 for Iteration #34 of Tilt-Series #3. CCMS(rotations) = 1.85844 for Iteration #34 of Tilt-Series #3. CCMS(scale) = 0.00578 for Iteration #34 of Tilt-Series #3. The scaled sum of CCMS values is #0.03517 for iteration 34 of Tilt-Series #3. Alignment quality for Iteration #34 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #34 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #34 in Parallel... Creating Refinement tilt-series video for iteration #34... Generating Refinement reconstruction for iteration #34... No Round parameters changed for Iteration #35 of Tilt-Series #3 (tiltseries0003_thick50) Beginning Refinement Iteration #35, Round #4 of Tilt-Series #3 (tiltseries0003_thick50) No parameters were switched for Iteration #35 Creating quality assessment statistics... CCMS(shift) = 0.0258 for Iteration #35 of Tilt-Series #3. CCMS(rotations) = 1.93369 for Iteration #35 of Tilt-Series #3. CCMS(scale) = 0.00762 for Iteration #35 of Tilt-Series #3. The scaled sum of CCMS values is #0.03692 for iteration 35 of Tilt-Series #3. Alignment quality for Iteration #35 of Tilt-Series #3 (18mar15h): Bad (confidence: 100.000000%)Tilt model stability (with respect to the last 3 iterations) at Iteration #35 of Tilt-Series #3 (18mar15h): UnstableCreating Depiction Videos for Iteration #35 in Parallel... Creating Refinement tilt-series video for iteration #35... Generating Refinement reconstruction for iteration #35... Finished Refinement for Tilt-Series #3 (tiltseries0003_thick50)! Did everything blow up and now you're yelling at your computer screen? If so, kindly email Alex at anoble@nysbc.org explaining the issue and include this log file. If everything worked beautifully and you publish, please use the appropriate citations listed on the Appion webpage! You can also print out all citations by typing: protomo2aligner.py --citations End time: 2018yr03m16d-07hr24m20s