DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/17jan05d/protomo_alignments/tiltseries0007/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/17jan05d/protomo_alignments/tiltseries0007/defocus_estimation/series0007_tilts_negative.txt Output diagnostic file name: /gpfs/appion/anoble/17jan05d/protomo_alignments/tiltseries0007/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 2.0, 200.0, 0.07, 10000.0, 1.5 Angstroms/Pixel: 1.50 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 6.0 dFMin[A], dFMax[A], FStep: 40000.0, 60000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 36.0 41000.0 41000.0 46000.0 1 33.0 54000.0 53500.0 47000.0 2 30.0 55000.0 57500.0 50000.0 3 27.0 46000.0 52000.0 42500.0 4 24.0 50000.0 43000.0 47000.0 5 21.0 0.0 48500.0 59000.0 6 18.0 51500.0 60000.0 59500.0 7 15.0 60000.0 60000.0 0.0 8 12.0 60000.0 57500.0 0.0 9 9.0 60000.0 43500.0 56500.0 10 6.0 60000.0 41000.0 60000.0 11 3.0 58000.0 53000.0 40000.0 12 0.1 56500.0 60000.0 41000.0 13 -0.1 53000.0 58000.0 60000.0 14 -3.0 60000.0 45500.0 60000.0 15 -6.0 60000.0 40000.0 57500.0 16 -9.0 60000.0 60000.0 40000.0 17 -12.0 57000.0 60000.0 40500.0 18 -15.0 56500.0 60000.0 60000.0 19 -18.0 59500.0 59500.0 60000.0 20 -21.0 60000.0 54500.0 54500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 2112 3840 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right)