DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/17jan26c/protomo_alignments/tiltseries0008/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/17jan26c/protomo_alignments/tiltseries0008/defocus_estimation/series0008_tilts_negative.txt Output diagnostic file name: /gpfs/appion/anoble/17jan26c/protomo_alignments/tiltseries0008/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.1 Angstroms/Pixel: 1.10 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 4.4 dFMin[A], dFMax[A], FStep: 40000.0, 60000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 30.0 53000.0 52500.0 51500.0 1 22.5 -17720967168.0 53000.0 52500.0 2 15.0 52500.0 53000.0 53000.0 3 7.5 53000.0 53000.0 -17805901824.0 4 0.1 53000.0 53000.0 53000.0 5 -0.1 52500.0 53000.0 53000.0 6 -7.5 53000.0 53000.0 52000.0 7 -15.0 52000.0 53000.0 52000.0 8 -22.5 0.0 51500.0 52500.0 9 -30.0 50000.0 51000.0 50500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)