CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/600.mrc Output diagnostic file name Micrographs_0601/600.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/600.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2328.000 8802.000 Mean, RMS : 4388.363 220.5296 TITLE 1: emi2mrc: 600.emi Mon Jan 06 13:35:52 2014 TITLE 2: kV 300, Mag 78000x, Def 29700, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 289.241, Y 850.840, Z -25.961, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.03594 16000.00 10000.00 0.00 -0.03584 16200.00 10000.00 0.00 -0.03573 16400.00 10000.00 0.00 -0.03552 16600.00 10000.00 0.00 -0.03523 16800.00 10000.00 0.00 -0.03503 17000.00 10000.00 0.00 -0.03471 17200.00 10000.00 0.00 -0.03410 17400.00 10000.00 0.00 -0.03350 17600.00 10000.00 0.00 -0.03307 17800.00 10000.00 0.00 -0.03268 18000.00 10000.00 0.00 -0.03223 18200.00 10000.00 0.00 -0.03177 18400.00 10000.00 0.00 -0.03115 18600.00 10000.00 0.00 -0.03038 18800.00 10000.00 0.00 -0.02981 19000.00 10000.00 0.00 -0.02923 19200.00 10000.00 0.00 -0.02846 19400.00 10000.00 0.00 -0.02770 19600.00 10000.00 0.00 -0.02681 19800.00 10000.00 0.00 -0.02583 20000.00 10000.00 0.00 -0.02490 20200.00 10000.00 0.00 -0.02399 20400.00 10000.00 0.00 -0.02308 20600.00 10000.00 0.00 -0.02207 20800.00 10000.00 0.00 -0.02110 21000.00 10000.00 0.00 -0.02019 21200.00 10000.00 0.00 -0.01923 21400.00 10000.00 0.00 -0.01837 21600.00 10000.00 0.00 -0.01753 21800.00 10000.00 0.00 -0.01667 22000.00 10000.00 0.00 -0.01570 22200.00 10000.00 0.00 -0.01464 22400.00 10000.00 0.00 -0.01372 22600.00 10000.00 0.00 -0.01273 22800.00 10000.00 0.00 -0.01162 23000.00 10000.00 0.00 -0.01049 23200.00 10000.00 0.00 -0.00932 23400.00 10000.00 0.00 -0.00810 23600.00 10000.00 0.00 -0.00696 23800.00 10000.00 0.00 -0.00600 24000.00 10000.00 0.00 -0.00497 24200.00 10000.00 0.00 -0.00381 24400.00 10000.00 0.00 -0.00244 24600.00 10000.00 0.00 -0.00091 24800.00 10000.00 0.00 0.00052 25000.00 10000.00 0.00 0.00209 25200.00 10000.00 0.00 0.00381 25400.00 10000.00 0.00 0.00553 25600.00 10000.00 0.00 0.00734 25800.00 10000.00 0.00 0.00934 26000.00 10000.00 0.00 0.01129 26200.00 10000.00 0.00 0.01303 26400.00 10000.00 0.00 0.01447 26600.00 10000.00 0.00 0.01536 26800.00 10000.00 0.00 0.01568 26800.00 10200.00 0.00 0.01592 26800.00 10400.00 0.00 0.01609 26800.00 10600.00 0.00 0.01612 26800.00 10800.00 0.00 0.01620 26600.00 11000.00 0.00 0.01630 26800.00 11000.00 0.00 0.01648 26600.00 11200.00 0.00 0.01660 26800.00 11200.00 0.00 0.01679 26600.00 11400.00 0.00 0.01690 26800.00 11400.00 0.00 0.01705 26600.00 11600.00 0.00 0.01716 26800.00 11600.00 0.00 0.01727 26600.00 11800.00 0.00 0.01740 26800.00 11800.00 0.00 0.01752 26600.00 12000.00 0.00 0.01767 26800.00 12000.00 0.00 0.01782 26600.00 12200.00 0.00 0.01807 26800.00 12200.00 0.00 0.01824 26600.00 12400.00 0.00 0.01863 26800.00 12400.00 0.00 0.01880 26600.00 12600.00 0.00 0.01921 26800.00 12600.00 0.00 0.01937 26600.00 12800.00 0.00 0.01967 26800.00 12800.00 0.00 0.01981 26600.00 13000.00 0.00 0.01993 26800.00 13000.00 0.00 0.02008 26600.00 13200.00 0.00 0.02023 26800.00 13200.00 0.00 0.02041 26600.00 13400.00 0.00 0.02077 26800.00 13400.00 0.00 0.02097 26600.00 13600.00 0.00 0.02139 26800.00 13600.00 0.00 0.02156 26600.00 13800.00 0.00 0.02195 26800.00 13800.00 0.00 0.02207 26600.00 14000.00 0.00 0.02253 26800.00 14000.00 0.00 0.02262 26600.00 14200.00 0.00 0.02308 26800.00 14200.00 0.00 0.02320 26600.00 14400.00 0.00 0.02360 26800.00 14400.00 0.00 0.02374 26600.00 14600.00 0.00 0.02415 26800.00 14600.00 0.00 0.02430 26600.00 14800.00 0.00 0.02484 26800.00 14800.00 0.00 0.02497 26600.00 15000.00 0.00 0.02569 26800.00 15000.00 0.00 0.02578 26600.00 15200.00 0.00 0.02641 26800.00 15200.00 0.00 0.02646 26600.00 15400.00 0.00 0.02704 26800.00 15400.00 0.00 0.02706 26400.00 15600.00 0.00 0.02715 26600.00 15600.00 0.00 0.02779 26800.00 15600.00 0.00 0.02780 26400.00 15800.00 0.00 0.02795 26600.00 15800.00 0.00 0.02859 26400.00 16000.00 0.00 0.02878 26600.00 16000.00 0.00 0.02939 26400.00 16200.00 0.00 0.02971 26600.00 16200.00 0.00 0.03030 26400.00 16400.00 0.00 0.03069 26600.00 16400.00 0.00 0.03127 26400.00 16600.00 0.00 0.03166 26600.00 16600.00 0.00 0.03224 26400.00 16800.00 0.00 0.03265 26600.00 16800.00 0.00 0.03324 26400.00 17000.00 0.00 0.03350 26600.00 17000.00 0.00 0.03405 26400.00 17200.00 0.00 0.03436 26600.00 17200.00 0.00 0.03488 26400.00 17400.00 0.00 0.03540 26600.00 17400.00 0.00 0.03591 26400.00 17600.00 0.00 0.03641 26600.00 17600.00 0.00 0.03689 26400.00 17800.00 0.00 0.03736 26600.00 17800.00 0.00 0.03778 26400.00 18000.00 0.00 0.03820 26600.00 18000.00 0.00 0.03861 26400.00 18200.00 0.00 0.03915 26600.00 18200.00 0.00 0.03955 26400.00 18400.00 0.00 0.04022 26600.00 18400.00 0.00 0.04064 26400.00 18600.00 0.00 0.04140 26600.00 18600.00 0.00 0.04182 26400.00 18800.00 0.00 0.04256 26600.00 18800.00 0.00 0.04297 26400.00 19000.00 0.00 0.04367 26600.00 19000.00 0.00 0.04407 26400.00 19200.00 0.00 0.04489 26600.00 19200.00 0.00 0.04523 26400.00 19400.00 0.00 0.04598 26600.00 19400.00 0.00 0.04625 26400.00 19600.00 0.00 0.04707 26600.00 19600.00 0.00 0.04734 26400.00 19800.00 0.00 0.04821 26600.00 19800.00 0.00 0.04847 26400.00 20000.00 0.00 0.04930 26600.00 20000.00 0.00 0.04950 26400.00 20200.00 0.00 0.05031 26600.00 20200.00 0.00 0.05043 26400.00 20400.00 0.00 0.05125 26600.00 20400.00 0.00 0.05130 26200.00 20600.00 0.00 0.05130 26400.00 20600.00 0.00 0.05240 26200.00 20800.00 0.00 0.05246 26400.00 20800.00 0.00 0.05346 26400.00 21000.00 0.00 0.05433 26200.00 21200.00 0.00 0.05436 26400.00 21200.00 0.00 0.05526 26200.00 21400.00 0.00 0.05540 26400.00 21400.00 0.00 0.05630 26200.00 21600.00 0.00 0.05632 26400.00 21600.00 0.00 0.05722 26200.00 21800.00 0.00 0.05728 26400.00 21800.00 0.00 0.05820 26200.00 22000.00 0.00 0.05850 26400.00 22000.00 0.00 0.05944 26200.00 22200.00 0.00 0.05969 26400.00 22200.00 0.00 0.06058 26200.00 22400.00 0.00 0.06099 26400.00 22400.00 0.00 0.06183 26200.00 22600.00 0.00 0.06239 26400.00 22600.00 0.00 0.06319 26200.00 22800.00 0.00 0.06378 26400.00 22800.00 0.00 0.06455 26200.00 23000.00 0.00 0.06504 26400.00 23000.00 0.00 0.06575 26200.00 23200.00 0.00 0.06616 26400.00 23200.00 0.00 0.06680 26200.00 23400.00 0.00 0.06727 26400.00 23400.00 0.00 0.06783 26200.00 23600.00 0.00 0.06829 26400.00 23600.00 0.00 0.06881 26200.00 23800.00 0.00 0.06955 26400.00 23800.00 0.00 0.07006 26200.00 24000.00 0.00 0.07086 26400.00 24000.00 0.00 0.07133 26200.00 24200.00 0.00 0.07201 26400.00 24200.00 0.00 0.07242 26200.00 24400.00 0.00 0.07307 26400.00 24400.00 0.00 0.07345 26200.00 24600.00 0.00 0.07432 26400.00 24600.00 0.00 0.07464 26200.00 24800.00 0.00 0.07549 26400.00 24800.00 0.00 0.07570 26200.00 25000.00 0.00 0.07652 26400.00 25000.00 0.00 0.07659 26200.00 25200.00 0.00 0.07776 26200.00 25400.00 0.00 0.07890 26200.00 25600.00 0.00 0.08000 26200.00 25800.00 0.00 0.08084 26200.00 26000.00 0.00 0.08161 26200.00 26200.00 0.00 0.08215 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 26115.57 26354.62 33.38 0.08238 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/600.ctf NX, NY, NZ: 512 512 1 MODE : real