CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/596.mrc Output diagnostic file name Micrographs_0601/596.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/596.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2202.000 9138.000 Mean, RMS : 4475.322 222.9142 TITLE 1: emi2mrc: 596.emi Mon Jan 06 13:58:47 2014 TITLE 2: kV 300, Mag 78000x, Def 25430, Spot 4, Int 55.05%, Obj 88.10%, Dif 73.67% TITLE 3: X 294.620, Y 860.104, Z -25.472, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01998 11600.00 10000.00 0.00 -0.01997 11800.00 10000.00 0.00 -0.01975 12000.00 10000.00 0.00 -0.01965 12200.00 10000.00 0.00 -0.01946 12400.00 10000.00 0.00 -0.01926 12600.00 10000.00 0.00 -0.01899 12800.00 10000.00 0.00 -0.01868 13000.00 10000.00 0.00 -0.01844 13200.00 10000.00 0.00 -0.01820 13400.00 10000.00 0.00 -0.01798 13600.00 10000.00 0.00 -0.01777 13800.00 10000.00 0.00 -0.01738 14000.00 10000.00 0.00 -0.01693 14200.00 10000.00 0.00 -0.01665 14400.00 10000.00 0.00 -0.01609 14600.00 10000.00 0.00 -0.01545 14800.00 10000.00 0.00 -0.01503 15000.00 10000.00 0.00 -0.01455 15200.00 10000.00 0.00 -0.01402 15400.00 10000.00 0.00 -0.01329 15600.00 10000.00 0.00 -0.01245 15800.00 10000.00 0.00 -0.01163 16000.00 10000.00 0.00 -0.01081 16200.00 10000.00 0.00 -0.00989 16400.00 10000.00 0.00 -0.00888 16600.00 10000.00 0.00 -0.00793 16800.00 10000.00 0.00 -0.00695 17000.00 10000.00 0.00 -0.00611 17200.00 10000.00 0.00 -0.00532 17400.00 10000.00 0.00 -0.00435 17600.00 10000.00 0.00 -0.00337 17800.00 10000.00 0.00 -0.00230 18000.00 10000.00 0.00 -0.00120 18200.00 10000.00 0.00 -0.00014 18400.00 10000.00 0.00 0.00104 18600.00 10000.00 0.00 0.00216 18800.00 10000.00 0.00 0.00331 19000.00 10000.00 0.00 0.00451 19200.00 10000.00 0.00 0.00564 19400.00 10000.00 0.00 0.00672 19600.00 10000.00 0.00 0.00783 19800.00 10000.00 0.00 0.00903 20000.00 10000.00 0.00 0.01021 20200.00 10000.00 0.00 0.01143 20400.00 10000.00 0.00 0.01269 20600.00 10000.00 0.00 0.01402 20800.00 10000.00 0.00 0.01544 21000.00 10000.00 0.00 0.01694 21200.00 10000.00 0.00 0.01854 21400.00 10000.00 0.00 0.02019 21600.00 10000.00 0.00 0.02185 21800.00 10000.00 0.00 0.02364 22000.00 10000.00 0.00 0.02566 22200.00 10000.00 0.00 0.02767 22400.00 10000.00 0.00 0.02960 22600.00 10000.00 0.00 0.03139 22800.00 10000.00 0.00 0.03279 23000.00 10000.00 0.00 0.03389 23200.00 10000.00 0.00 0.03464 23400.00 10000.00 0.00 0.03508 23600.00 10000.00 0.00 0.03518 23400.00 10200.00 0.00 0.03556 23600.00 10200.00 0.00 0.03566 23400.00 10400.00 0.00 0.03602 23600.00 10400.00 0.00 0.03610 23400.00 10600.00 0.00 0.03640 23600.00 10600.00 0.00 0.03648 23400.00 10800.00 0.00 0.03682 23600.00 10800.00 0.00 0.03693 23400.00 11000.00 0.00 0.03731 23600.00 11000.00 0.00 0.03740 23400.00 11200.00 0.00 0.03788 23600.00 11200.00 0.00 0.03794 23200.00 11400.00 0.00 0.03796 23400.00 11400.00 0.00 0.03840 23400.00 11600.00 0.00 0.03871 23200.00 11800.00 0.00 0.03881 23400.00 11800.00 0.00 0.03918 23200.00 12000.00 0.00 0.03937 23400.00 12000.00 0.00 0.03971 23200.00 12200.00 0.00 0.03998 23400.00 12200.00 0.00 0.04028 23200.00 12400.00 0.00 0.04078 23400.00 12400.00 0.00 0.04105 23200.00 12600.00 0.00 0.04145 23400.00 12600.00 0.00 0.04169 23200.00 12800.00 0.00 0.04214 23400.00 12800.00 0.00 0.04238 23200.00 13000.00 0.00 0.04292 23400.00 13000.00 0.00 0.04320 23200.00 13200.00 0.00 0.04365 23400.00 13200.00 0.00 0.04396 23200.00 13400.00 0.00 0.04441 23400.00 13400.00 0.00 0.04475 23600.00 13400.00 0.00 0.04476 23200.00 13600.00 0.00 0.04531 23400.00 13600.00 0.00 0.04568 23600.00 13600.00 0.00 0.04568 23200.00 13800.00 0.00 0.04633 23400.00 13800.00 0.00 0.04673 23200.00 14000.00 0.00 0.04742 23400.00 14000.00 0.00 0.04784 23000.00 14200.00 0.00 0.04787 23200.00 14200.00 0.00 0.04862 23400.00 14200.00 0.00 0.04900 23000.00 14400.00 0.00 0.04905 23200.00 14400.00 0.00 0.04974 23400.00 14400.00 0.00 0.05004 23000.00 14600.00 0.00 0.05004 23200.00 14600.00 0.00 0.05070 23400.00 14600.00 0.00 0.05095 23000.00 14800.00 0.00 0.05103 23200.00 14800.00 0.00 0.05170 23400.00 14800.00 0.00 0.05195 23000.00 15000.00 0.00 0.05212 23200.00 15000.00 0.00 0.05275 23400.00 15000.00 0.00 0.05295 23000.00 15200.00 0.00 0.05300 23200.00 15200.00 0.00 0.05358 23400.00 15200.00 0.00 0.05374 23000.00 15400.00 0.00 0.05389 23200.00 15400.00 0.00 0.05447 23400.00 15400.00 0.00 0.05465 23000.00 15600.00 0.00 0.05500 23200.00 15600.00 0.00 0.05560 23400.00 15600.00 0.00 0.05579 23000.00 15800.00 0.00 0.05612 23200.00 15800.00 0.00 0.05672 23400.00 15800.00 0.00 0.05689 23000.00 16000.00 0.00 0.05721 23200.00 16000.00 0.00 0.05781 23400.00 16000.00 0.00 0.05796 23000.00 16200.00 0.00 0.05828 23200.00 16200.00 0.00 0.05889 23400.00 16200.00 0.00 0.05904 23000.00 16400.00 0.00 0.05958 23200.00 16400.00 0.00 0.06020 23400.00 16400.00 0.00 0.06035 23000.00 16600.00 0.00 0.06095 23200.00 16600.00 0.00 0.06155 23400.00 16600.00 0.00 0.06166 23000.00 16800.00 0.00 0.06235 23200.00 16800.00 0.00 0.06290 23400.00 16800.00 0.00 0.06295 23000.00 17000.00 0.00 0.06373 23200.00 17000.00 0.00 0.06425 23400.00 17000.00 0.00 0.06428 23000.00 17200.00 0.00 0.06498 23200.00 17200.00 0.00 0.06548 23400.00 17200.00 0.00 0.06549 23000.00 17400.00 0.00 0.06641 23200.00 17400.00 0.00 0.06688 23000.00 17600.00 0.00 0.06791 23200.00 17600.00 0.00 0.06834 23000.00 17800.00 0.00 0.06935 23200.00 17800.00 0.00 0.06973 23000.00 18000.00 0.00 0.07067 23200.00 18000.00 0.00 0.07101 23000.00 18200.00 0.00 0.07203 23200.00 18200.00 0.00 0.07233 22800.00 18400.00 0.00 0.07235 23000.00 18400.00 0.00 0.07336 23200.00 18400.00 0.00 0.07361 23000.00 18600.00 0.00 0.07460 23200.00 18600.00 0.00 0.07486 22800.00 18800.00 0.00 0.07497 23000.00 18800.00 0.00 0.07602 23200.00 18800.00 0.00 0.07633 22800.00 19000.00 0.00 0.07651 23000.00 19000.00 0.00 0.07762 23200.00 19000.00 0.00 0.07791 22800.00 19200.00 0.00 0.07824 23000.00 19200.00 0.00 0.07933 23200.00 19200.00 0.00 0.07954 22800.00 19400.00 0.00 0.07993 23000.00 19400.00 0.00 0.08098 23200.00 19400.00 0.00 0.08113 22800.00 19600.00 0.00 0.08171 23000.00 19600.00 0.00 0.08276 23200.00 19600.00 0.00 0.08292 22800.00 19800.00 0.00 0.08358 23000.00 19800.00 0.00 0.08466 23200.00 19800.00 0.00 0.08478 22800.00 20000.00 0.00 0.08554 23000.00 20000.00 0.00 0.08658 22800.00 20200.00 0.00 0.08747 23000.00 20200.00 0.00 0.08841 22800.00 20400.00 0.00 0.08934 23000.00 20400.00 0.00 0.09021 22800.00 20600.00 0.00 0.09133 23000.00 20600.00 0.00 0.09214 22800.00 20800.00 0.00 0.09322 23000.00 20800.00 0.00 0.09395 22800.00 21000.00 0.00 0.09528 23000.00 21000.00 0.00 0.09588 22800.00 21200.00 0.00 0.09727 23000.00 21200.00 0.00 0.09775 22800.00 21400.00 0.00 0.09936 23000.00 21400.00 0.00 0.09973 22600.00 21600.00 0.00 0.09996 22800.00 21600.00 0.00 0.10156 23000.00 21600.00 0.00 0.10181 22600.00 21800.00 0.00 0.10218 22800.00 21800.00 0.00 0.10370 23000.00 21800.00 0.00 0.10380 22600.00 22000.00 0.00 0.10462 22800.00 22000.00 0.00 0.10602 22600.00 22200.00 0.00 0.10705 22800.00 22200.00 0.00 0.10829 22600.00 22400.00 0.00 0.10990 22800.00 22400.00 0.00 0.11081 22600.00 22600.00 0.00 0.11231 22800.00 22600.00 0.00 0.11285 22600.00 22800.00 0.00 0.11376 22800.00 22800.00 0.00 0.11390 22600.00 22800.00 10.00 0.11392 22600.00 22800.00 15.00 0.11398 22600.00 22800.00 20.00 0.11402 22600.00 22800.00 25.00 0.11403 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22644.26 22881.84 20.68 0.11428 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/596.ctf NX, NY, NZ: 512 512 1 MODE : real