CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/595.mrc Output diagnostic file name Micrographs_0601/595.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/595.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2166.000 8334.000 Mean, RMS : 4530.354 217.2224 TITLE 1: emi2mrc: 595.emi Mon Jan 06 19:22:32 2014 TITLE 2: kV 300, Mag 78000x, Def 25270, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -33.334, Y 219.650, Z -42.241, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02860 14600.00 10000.00 0.00 -0.02847 14800.00 10000.00 0.00 -0.02816 15000.00 10000.00 0.00 -0.02778 15200.00 10000.00 0.00 -0.02745 15400.00 10000.00 0.00 -0.02709 15600.00 10000.00 0.00 -0.02669 15800.00 10000.00 0.00 -0.02626 16000.00 10000.00 0.00 -0.02581 16200.00 10000.00 0.00 -0.02531 16400.00 10000.00 0.00 -0.02472 16600.00 10000.00 0.00 -0.02413 16800.00 10000.00 0.00 -0.02357 17000.00 10000.00 0.00 -0.02308 17200.00 10000.00 0.00 -0.02264 17400.00 10000.00 0.00 -0.02217 17600.00 10000.00 0.00 -0.02164 17800.00 10000.00 0.00 -0.02099 18000.00 10000.00 0.00 -0.02036 18200.00 10000.00 0.00 -0.01978 18400.00 10000.00 0.00 -0.01912 18600.00 10000.00 0.00 -0.01845 18800.00 10000.00 0.00 -0.01753 19000.00 10000.00 0.00 -0.01647 19200.00 10000.00 0.00 -0.01556 19400.00 10000.00 0.00 -0.01466 19600.00 10000.00 0.00 -0.01378 19800.00 10000.00 0.00 -0.01280 20000.00 10000.00 0.00 -0.01188 20200.00 10000.00 0.00 -0.01115 20400.00 10000.00 0.00 -0.01049 20600.00 10000.00 0.00 -0.00988 20800.00 10000.00 0.00 -0.00907 21000.00 10000.00 0.00 -0.00813 21200.00 10000.00 0.00 -0.00728 21400.00 10000.00 0.00 -0.00640 21600.00 10000.00 0.00 -0.00544 21800.00 10000.00 0.00 -0.00437 22000.00 10000.00 0.00 -0.00323 22200.00 10000.00 0.00 -0.00237 22400.00 10000.00 0.00 -0.00153 22600.00 10000.00 0.00 -0.00043 22800.00 10000.00 0.00 0.00053 23000.00 10000.00 0.00 0.00153 23200.00 10000.00 0.00 0.00281 23400.00 10000.00 0.00 0.00404 23600.00 10000.00 0.00 0.00531 23800.00 10000.00 0.00 0.00691 24000.00 10000.00 0.00 0.00860 24200.00 10000.00 0.00 0.01026 24400.00 10000.00 0.00 0.01192 24600.00 10000.00 0.00 0.01351 24800.00 10000.00 0.00 0.01520 25000.00 10000.00 0.00 0.01688 25200.00 10000.00 0.00 0.01861 25400.00 10000.00 0.00 0.02035 25600.00 10000.00 0.00 0.02177 25800.00 10000.00 0.00 0.02277 26000.00 10000.00 0.00 0.02317 26200.00 10000.00 0.00 0.02327 26400.00 10000.00 0.00 0.02329 26600.00 10000.00 0.00 0.02331 26000.00 10200.00 0.00 0.02341 26200.00 10200.00 0.00 0.02353 26400.00 10200.00 0.00 0.02357 26600.00 10200.00 0.00 0.02360 26000.00 10400.00 0.00 0.02367 26200.00 10400.00 0.00 0.02384 26400.00 10400.00 0.00 0.02391 26600.00 10400.00 0.00 0.02392 26200.00 10600.00 0.00 0.02407 26400.00 10600.00 0.00 0.02411 26200.00 10800.00 0.00 0.02418 26200.00 11000.00 0.00 0.02426 26200.00 11200.00 0.00 0.02439 26000.00 11400.00 0.00 0.02450 26200.00 11400.00 0.00 0.02461 26000.00 11600.00 0.00 0.02490 26200.00 11600.00 0.00 0.02499 25800.00 11800.00 0.00 0.02501 26000.00 11800.00 0.00 0.02538 26200.00 11800.00 0.00 0.02544 26000.00 12000.00 0.00 0.02570 26200.00 12000.00 0.00 0.02575 26000.00 12200.00 0.00 0.02603 26200.00 12200.00 0.00 0.02607 25800.00 12400.00 0.00 0.02615 26000.00 12400.00 0.00 0.02644 25800.00 12600.00 0.00 0.02652 26000.00 12600.00 0.00 0.02679 25800.00 12800.00 0.00 0.02687 26000.00 12800.00 0.00 0.02715 26200.00 12800.00 0.00 0.02715 25800.00 13000.00 0.00 0.02732 26000.00 13000.00 0.00 0.02763 26200.00 13000.00 0.00 0.02763 25800.00 13200.00 0.00 0.02786 26000.00 13200.00 0.00 0.02813 25800.00 13400.00 0.00 0.02839 26000.00 13400.00 0.00 0.02861 26600.00 13400.00 0.00 0.02864 25800.00 13600.00 0.00 0.02896 26000.00 13600.00 0.00 0.02917 26200.00 13600.00 0.00 0.02924 26400.00 13600.00 0.00 0.02931 26600.00 13600.00 0.00 0.02940 25800.00 13800.00 0.00 0.02955 26000.00 13800.00 0.00 0.02981 26200.00 13800.00 0.00 0.02998 26400.00 13800.00 0.00 0.03010 26600.00 13800.00 0.00 0.03018 25800.00 14000.00 0.00 0.03023 26000.00 14000.00 0.00 0.03054 26200.00 14000.00 0.00 0.03074 26400.00 14000.00 0.00 0.03085 26600.00 14000.00 0.00 0.03090 26000.00 14200.00 0.00 0.03114 26200.00 14200.00 0.00 0.03133 26400.00 14200.00 0.00 0.03142 26600.00 14200.00 0.00 0.03143 26000.00 14400.00 0.00 0.03167 26200.00 14400.00 0.00 0.03187 26400.00 14400.00 0.00 0.03195 26600.00 14400.00 0.00 0.03196 25800.00 14600.00 0.00 0.03207 26000.00 14600.00 0.00 0.03236 26200.00 14600.00 0.00 0.03261 26400.00 14600.00 0.00 0.03271 25800.00 14800.00 0.00 0.03285 26000.00 14800.00 0.00 0.03321 26200.00 14800.00 0.00 0.03353 26400.00 14800.00 0.00 0.03363 25800.00 15000.00 0.00 0.03389 26000.00 15000.00 0.00 0.03430 26200.00 15000.00 0.00 0.03461 26400.00 15000.00 0.00 0.03466 25800.00 15200.00 0.00 0.03474 26000.00 15200.00 0.00 0.03513 26200.00 15200.00 0.00 0.03542 26400.00 15200.00 0.00 0.03544 26000.00 15400.00 0.00 0.03581 26200.00 15400.00 0.00 0.03615 26400.00 15400.00 0.00 0.03620 26000.00 15600.00 0.00 0.03672 26200.00 15600.00 0.00 0.03709 26400.00 15600.00 0.00 0.03711 26000.00 15800.00 0.00 0.03755 26200.00 15800.00 0.00 0.03784 26000.00 16000.00 0.00 0.03836 26200.00 16000.00 0.00 0.03857 25800.00 16200.00 0.00 0.03876 26000.00 16200.00 0.00 0.03924 26200.00 16200.00 0.00 0.03940 25800.00 16400.00 0.00 0.03968 26000.00 16400.00 0.00 0.04011 26200.00 16400.00 0.00 0.04025 25800.00 16600.00 0.00 0.04036 26000.00 16600.00 0.00 0.04078 26200.00 16600.00 0.00 0.04094 25800.00 16800.00 0.00 0.04104 26000.00 16800.00 0.00 0.04151 26200.00 16800.00 0.00 0.04171 25800.00 17000.00 0.00 0.04194 26000.00 17000.00 0.00 0.04245 26200.00 17000.00 0.00 0.04265 25800.00 17200.00 0.00 0.04295 26000.00 17200.00 0.00 0.04346 26200.00 17200.00 0.00 0.04363 25800.00 17400.00 0.00 0.04414 26000.00 17400.00 0.00 0.04462 26200.00 17400.00 0.00 0.04476 25800.00 17600.00 0.00 0.04532 26000.00 17600.00 0.00 0.04577 26200.00 17600.00 0.00 0.04588 25800.00 17800.00 0.00 0.04632 26000.00 17800.00 0.00 0.04673 26200.00 17800.00 0.00 0.04682 25800.00 18000.00 0.00 0.04719 26000.00 18000.00 0.00 0.04759 26200.00 18000.00 0.00 0.04764 25800.00 18200.00 0.00 0.04824 26000.00 18200.00 0.00 0.04862 26200.00 18200.00 0.00 0.04863 25800.00 18400.00 0.00 0.04928 26000.00 18400.00 0.00 0.04964 25800.00 18600.00 0.00 0.05022 26000.00 18600.00 0.00 0.05057 25800.00 18800.00 0.00 0.05129 26000.00 18800.00 0.00 0.05163 25800.00 19000.00 0.00 0.05222 26000.00 19000.00 0.00 0.05254 25800.00 19200.00 0.00 0.05306 26000.00 19200.00 0.00 0.05339 25800.00 19400.00 0.00 0.05398 26000.00 19400.00 0.00 0.05434 25800.00 19600.00 0.00 0.05513 26000.00 19600.00 0.00 0.05550 25800.00 19800.00 0.00 0.05632 26000.00 19800.00 0.00 0.05666 25800.00 20000.00 0.00 0.05745 26000.00 20000.00 0.00 0.05778 25800.00 20200.00 0.00 0.05867 26000.00 20200.00 0.00 0.05906 25800.00 20400.00 0.00 0.06001 26000.00 20400.00 0.00 0.06040 25800.00 20600.00 0.00 0.06139 26000.00 20600.00 0.00 0.06174 25800.00 20800.00 0.00 0.06274 26000.00 20800.00 0.00 0.06304 25600.00 21000.00 0.00 0.06317 25800.00 21000.00 0.00 0.06425 26000.00 21000.00 0.00 0.06446 25600.00 21200.00 0.00 0.06468 25800.00 21200.00 0.00 0.06569 26000.00 21200.00 0.00 0.06580 25600.00 21400.00 0.00 0.06617 25800.00 21400.00 0.00 0.06709 26000.00 21400.00 0.00 0.06711 25600.00 21600.00 0.00 0.06760 25800.00 21600.00 0.00 0.06841 25600.00 21800.00 0.00 0.06877 25800.00 21800.00 0.00 0.06949 25600.00 22000.00 0.00 0.06997 25800.00 22000.00 0.00 0.07066 25600.00 22200.00 0.00 0.07121 25800.00 22200.00 0.00 0.07186 25600.00 22400.00 0.00 0.07251 25800.00 22400.00 0.00 0.07316 25600.00 22600.00 0.00 0.07382 25800.00 22600.00 0.00 0.07448 25600.00 22800.00 0.00 0.07507 25800.00 22800.00 0.00 0.07567 25600.00 23000.00 0.00 0.07623 25800.00 23000.00 0.00 0.07680 25600.00 23200.00 0.00 0.07746 25800.00 23200.00 0.00 0.07801 25600.00 23400.00 0.00 0.07878 25800.00 23400.00 0.00 0.07925 25600.00 23600.00 0.00 0.08007 25800.00 23600.00 0.00 0.08046 25600.00 23800.00 0.00 0.08134 25800.00 23800.00 0.00 0.08163 25600.00 24000.00 0.00 0.08247 25800.00 24000.00 0.00 0.08266 25600.00 24200.00 0.00 0.08364 25800.00 24200.00 0.00 0.08373 25600.00 24400.00 0.00 0.08476 25600.00 24600.00 0.00 0.08602 25600.00 24800.00 0.00 0.08711 25600.00 25000.00 0.00 0.08786 25400.00 25200.00 0.00 0.08801 25600.00 25200.00 0.00 0.08867 25400.00 25400.00 0.00 0.08882 25600.00 25400.00 0.00 0.08924 25400.00 25600.00 0.00 0.08945 25600.00 25600.00 0.00 0.08947 25400.00 25600.00 5.00 0.08952 25400.00 25600.00 10.00 0.08959 25400.00 25600.00 15.00 0.08966 25400.00 25600.00 20.00 0.08971 25400.00 25600.00 25.00 0.08975 25400.00 25600.00 30.00 0.08978 25400.00 25600.00 35.00 0.08980 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25452.94 25631.88 31.36 0.08988 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/595.ctf NX, NY, NZ: 512 512 1 MODE : real