CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/594.mrc Output diagnostic file name Micrographs_0601/594.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/594.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2640.000 8338.000 Mean, RMS : 4526.154 221.0984 TITLE 1: emi2mrc: 594.emi Mon Jan 06 19:21:55 2014 TITLE 2: kV 300, Mag 78000x, Def 25270, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -31.407, Y 217.221, Z -42.241, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01955 12000.00 10000.00 0.00 -0.01953 12200.00 10000.00 0.00 -0.01944 12400.00 10000.00 0.00 -0.01936 12600.00 10000.00 0.00 -0.01921 12800.00 10000.00 0.00 -0.01906 13000.00 10000.00 0.00 -0.01894 13200.00 10000.00 0.00 -0.01883 13400.00 10000.00 0.00 -0.01877 13600.00 10000.00 0.00 -0.01855 13800.00 10000.00 0.00 -0.01824 14000.00 10000.00 0.00 -0.01789 14200.00 10000.00 0.00 -0.01758 14400.00 10000.00 0.00 -0.01728 14600.00 10000.00 0.00 -0.01683 14800.00 10000.00 0.00 -0.01625 15000.00 10000.00 0.00 -0.01558 15200.00 10000.00 0.00 -0.01512 15400.00 10000.00 0.00 -0.01468 15600.00 10000.00 0.00 -0.01404 15800.00 10000.00 0.00 -0.01335 16000.00 10000.00 0.00 -0.01258 16200.00 10000.00 0.00 -0.01182 16400.00 10000.00 0.00 -0.01104 16600.00 10000.00 0.00 -0.01018 16800.00 10000.00 0.00 -0.00918 17000.00 10000.00 0.00 -0.00807 17200.00 10000.00 0.00 -0.00716 17400.00 10000.00 0.00 -0.00633 17600.00 10000.00 0.00 -0.00552 17800.00 10000.00 0.00 -0.00469 18000.00 10000.00 0.00 -0.00380 18200.00 10000.00 0.00 -0.00283 18400.00 10000.00 0.00 -0.00169 18600.00 10000.00 0.00 -0.00067 18800.00 10000.00 0.00 0.00033 19000.00 10000.00 0.00 0.00150 19200.00 10000.00 0.00 0.00272 19400.00 10000.00 0.00 0.00373 19600.00 10000.00 0.00 0.00462 19800.00 10000.00 0.00 0.00571 20000.00 10000.00 0.00 0.00692 20200.00 10000.00 0.00 0.00818 20400.00 10000.00 0.00 0.00948 20600.00 10000.00 0.00 0.01071 20800.00 10000.00 0.00 0.01187 21000.00 10000.00 0.00 0.01294 21200.00 10000.00 0.00 0.01402 21400.00 10000.00 0.00 0.01525 21600.00 10000.00 0.00 0.01660 21800.00 10000.00 0.00 0.01804 22000.00 10000.00 0.00 0.01955 22200.00 10000.00 0.00 0.02104 22400.00 10000.00 0.00 0.02251 22600.00 10000.00 0.00 0.02405 22800.00 10000.00 0.00 0.02573 23000.00 10000.00 0.00 0.02740 23200.00 10000.00 0.00 0.02886 23400.00 10000.00 0.00 0.03001 23600.00 10000.00 0.00 0.03086 23800.00 10000.00 0.00 0.03144 24000.00 10000.00 0.00 0.03170 24200.00 10000.00 0.00 0.03179 24000.00 10200.00 0.00 0.03201 24200.00 10200.00 0.00 0.03209 23800.00 10400.00 0.00 0.03223 24000.00 10400.00 0.00 0.03246 24200.00 10400.00 0.00 0.03254 24000.00 10600.00 0.00 0.03274 24200.00 10600.00 0.00 0.03282 23800.00 10800.00 0.00 0.03289 24000.00 10800.00 0.00 0.03312 24200.00 10800.00 0.00 0.03323 23800.00 11000.00 0.00 0.03340 24000.00 11000.00 0.00 0.03365 24200.00 11000.00 0.00 0.03376 23800.00 11200.00 0.00 0.03379 24000.00 11200.00 0.00 0.03405 24200.00 11200.00 0.00 0.03415 23800.00 11400.00 0.00 0.03434 24000.00 11400.00 0.00 0.03459 24200.00 11400.00 0.00 0.03467 23800.00 11600.00 0.00 0.03497 24000.00 11600.00 0.00 0.03517 24200.00 11600.00 0.00 0.03521 23800.00 11800.00 0.00 0.03552 24000.00 11800.00 0.00 0.03567 24200.00 11800.00 0.00 0.03572 23800.00 12000.00 0.00 0.03606 24000.00 12000.00 0.00 0.03623 24200.00 12000.00 0.00 0.03634 23800.00 12200.00 0.00 0.03666 24000.00 12200.00 0.00 0.03688 24200.00 12200.00 0.00 0.03703 23800.00 12400.00 0.00 0.03734 24000.00 12400.00 0.00 0.03758 24200.00 12400.00 0.00 0.03771 23800.00 12600.00 0.00 0.03807 24000.00 12600.00 0.00 0.03831 24200.00 12600.00 0.00 0.03840 23600.00 12800.00 0.00 0.03844 23800.00 12800.00 0.00 0.03880 24000.00 12800.00 0.00 0.03903 24200.00 12800.00 0.00 0.03910 23800.00 13000.00 0.00 0.03942 24000.00 13000.00 0.00 0.03966 24200.00 13000.00 0.00 0.03972 23800.00 13200.00 0.00 0.04006 24000.00 13200.00 0.00 0.04032 24200.00 13200.00 0.00 0.04037 23800.00 13400.00 0.00 0.04077 24000.00 13400.00 0.00 0.04102 24200.00 13400.00 0.00 0.04102 23600.00 13600.00 0.00 0.04104 23800.00 13600.00 0.00 0.04146 24000.00 13600.00 0.00 0.04168 23600.00 13800.00 0.00 0.04186 23800.00 13800.00 0.00 0.04228 24000.00 13800.00 0.00 0.04249 23600.00 14000.00 0.00 0.04293 23800.00 14000.00 0.00 0.04333 24000.00 14000.00 0.00 0.04352 23600.00 14200.00 0.00 0.04399 23800.00 14200.00 0.00 0.04435 24000.00 14200.00 0.00 0.04448 23600.00 14400.00 0.00 0.04497 23800.00 14400.00 0.00 0.04527 24000.00 14400.00 0.00 0.04540 23600.00 14600.00 0.00 0.04595 23800.00 14600.00 0.00 0.04623 24000.00 14600.00 0.00 0.04638 24200.00 14600.00 0.00 0.04639 23600.00 14800.00 0.00 0.04688 23800.00 14800.00 0.00 0.04717 24000.00 14800.00 0.00 0.04737 24200.00 14800.00 0.00 0.04739 23600.00 15000.00 0.00 0.04796 23800.00 15000.00 0.00 0.04826 24000.00 15000.00 0.00 0.04847 23600.00 15200.00 0.00 0.04898 23800.00 15200.00 0.00 0.04926 24000.00 15200.00 0.00 0.04946 23600.00 15400.00 0.00 0.05000 23800.00 15400.00 0.00 0.05028 24000.00 15400.00 0.00 0.05046 23400.00 15600.00 0.00 0.05049 23600.00 15600.00 0.00 0.05116 23800.00 15600.00 0.00 0.05143 24000.00 15600.00 0.00 0.05158 23400.00 15800.00 0.00 0.05160 23600.00 15800.00 0.00 0.05224 23800.00 15800.00 0.00 0.05248 24000.00 15800.00 0.00 0.05260 23400.00 16000.00 0.00 0.05271 23600.00 16000.00 0.00 0.05331 23800.00 16000.00 0.00 0.05354 24000.00 16000.00 0.00 0.05368 23400.00 16200.00 0.00 0.05375 23600.00 16200.00 0.00 0.05431 23800.00 16200.00 0.00 0.05455 24000.00 16200.00 0.00 0.05472 23400.00 16400.00 0.00 0.05493 23600.00 16400.00 0.00 0.05547 23800.00 16400.00 0.00 0.05574 24000.00 16400.00 0.00 0.05590 23400.00 16600.00 0.00 0.05622 23600.00 16600.00 0.00 0.05677 23800.00 16600.00 0.00 0.05707 24000.00 16600.00 0.00 0.05718 23400.00 16800.00 0.00 0.05751 23600.00 16800.00 0.00 0.05809 23800.00 16800.00 0.00 0.05839 24000.00 16800.00 0.00 0.05845 23400.00 17000.00 0.00 0.05886 23600.00 17000.00 0.00 0.05942 23800.00 17000.00 0.00 0.05966 24000.00 17000.00 0.00 0.05968 23400.00 17200.00 0.00 0.06009 23600.00 17200.00 0.00 0.06059 23800.00 17200.00 0.00 0.06080 24000.00 17200.00 0.00 0.06084 23400.00 17400.00 0.00 0.06128 23600.00 17400.00 0.00 0.06180 23800.00 17400.00 0.00 0.06207 24000.00 17400.00 0.00 0.06218 23400.00 17600.00 0.00 0.06255 23600.00 17600.00 0.00 0.06314 23800.00 17600.00 0.00 0.06347 24000.00 17600.00 0.00 0.06357 23400.00 17800.00 0.00 0.06396 23600.00 17800.00 0.00 0.06457 23800.00 17800.00 0.00 0.06487 24000.00 17800.00 0.00 0.06490 23400.00 18000.00 0.00 0.06535 23600.00 18000.00 0.00 0.06593 23800.00 18000.00 0.00 0.06619 23400.00 18200.00 0.00 0.06683 23600.00 18200.00 0.00 0.06736 23800.00 18200.00 0.00 0.06757 23400.00 18400.00 0.00 0.06831 23600.00 18400.00 0.00 0.06883 23800.00 18400.00 0.00 0.06902 23400.00 18600.00 0.00 0.06979 23600.00 18600.00 0.00 0.07033 23800.00 18600.00 0.00 0.07051 23400.00 18800.00 0.00 0.07148 23600.00 18800.00 0.00 0.07201 23800.00 18800.00 0.00 0.07214 23400.00 19000.00 0.00 0.07319 23600.00 19000.00 0.00 0.07364 23800.00 19000.00 0.00 0.07371 23400.00 19200.00 0.00 0.07492 23600.00 19200.00 0.00 0.07529 23800.00 19200.00 0.00 0.07531 23200.00 19400.00 0.00 0.07533 23400.00 19400.00 0.00 0.07656 23600.00 19400.00 0.00 0.07689 23800.00 19400.00 0.00 0.07692 23200.00 19600.00 0.00 0.07713 23400.00 19600.00 0.00 0.07832 23600.00 19600.00 0.00 0.07867 23800.00 19600.00 0.00 0.07868 23200.00 19800.00 0.00 0.07893 23400.00 19800.00 0.00 0.08007 23600.00 19800.00 0.00 0.08038 23200.00 20000.00 0.00 0.08055 23400.00 20000.00 0.00 0.08163 23600.00 20000.00 0.00 0.08188 23200.00 20200.00 0.00 0.08221 23400.00 20200.00 0.00 0.08331 23600.00 20200.00 0.00 0.08361 23200.00 20400.00 0.00 0.08396 23400.00 20400.00 0.00 0.08512 23600.00 20400.00 0.00 0.08543 23200.00 20600.00 0.00 0.08591 23400.00 20600.00 0.00 0.08706 23600.00 20600.00 0.00 0.08732 23200.00 20800.00 0.00 0.08781 23400.00 20800.00 0.00 0.08895 23600.00 20800.00 0.00 0.08917 23200.00 21000.00 0.00 0.08984 23400.00 21000.00 0.00 0.09099 23600.00 21000.00 0.00 0.09115 23200.00 21200.00 0.00 0.09195 23400.00 21200.00 0.00 0.09307 23600.00 21200.00 0.00 0.09310 23200.00 21400.00 0.00 0.09414 23400.00 21400.00 0.00 0.09518 23200.00 21600.00 0.00 0.09643 23400.00 21600.00 0.00 0.09738 23200.00 21800.00 0.00 0.09861 23400.00 21800.00 0.00 0.09946 23200.00 22000.00 0.00 0.10080 23400.00 22000.00 0.00 0.10157 23200.00 22200.00 0.00 0.10300 23400.00 22200.00 0.00 0.10366 23000.00 22400.00 0.00 0.10366 23200.00 22400.00 0.00 0.10524 23400.00 22400.00 0.00 0.10579 23000.00 22600.00 0.00 0.10589 23200.00 22600.00 0.00 0.10740 23400.00 22600.00 0.00 0.10785 23000.00 22800.00 0.00 0.10823 23200.00 22800.00 0.00 0.10963 23400.00 22800.00 0.00 0.10990 23000.00 23000.00 0.00 0.11023 23200.00 23000.00 0.00 0.11134 23000.00 23200.00 0.00 0.11135 23200.00 23200.00 0.00 0.11225 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23298.93 23168.60 -50.99 0.11248 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/594.ctf NX, NY, NZ: 512 512 1 MODE : real