CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/593.mrc Output diagnostic file name Micrographs_0601/593.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/593.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2478.000 8224.000 Mean, RMS : 4528.401 219.0110 TITLE 1: emi2mrc: 593.emi Mon Jan 06 19:21:14 2014 TITLE 2: kV 300, Mag 78000x, Def 25270, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -30.058, Y 215.323, Z -42.241, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01515 10200.00 10000.00 0.00 -0.01510 10400.00 10000.00 0.00 -0.01499 11000.00 10000.00 0.00 -0.01483 11200.00 10000.00 0.00 -0.01457 11400.00 10000.00 0.00 -0.01434 11600.00 10000.00 0.00 -0.01415 11800.00 10000.00 0.00 -0.01388 12000.00 10000.00 0.00 -0.01355 12200.00 10000.00 0.00 -0.01321 12400.00 10000.00 0.00 -0.01287 12600.00 10000.00 0.00 -0.01256 12800.00 10000.00 0.00 -0.01233 13000.00 10000.00 0.00 -0.01214 13200.00 10000.00 0.00 -0.01193 13400.00 10000.00 0.00 -0.01151 13600.00 10000.00 0.00 -0.01103 13800.00 10000.00 0.00 -0.01074 14000.00 10000.00 0.00 -0.01036 14200.00 10000.00 0.00 -0.00996 14400.00 10000.00 0.00 -0.00957 14600.00 10000.00 0.00 -0.00904 14800.00 10000.00 0.00 -0.00845 15000.00 10000.00 0.00 -0.00791 15200.00 10000.00 0.00 -0.00721 15400.00 10000.00 0.00 -0.00626 15600.00 10000.00 0.00 -0.00532 15800.00 10000.00 0.00 -0.00439 16000.00 10000.00 0.00 -0.00344 16200.00 10000.00 0.00 -0.00249 16400.00 10000.00 0.00 -0.00155 16600.00 10000.00 0.00 -0.00060 16800.00 10000.00 0.00 0.00043 17000.00 10000.00 0.00 0.00156 17200.00 10000.00 0.00 0.00287 17400.00 10000.00 0.00 0.00426 17600.00 10000.00 0.00 0.00553 17800.00 10000.00 0.00 0.00668 18000.00 10000.00 0.00 0.00783 18200.00 10000.00 0.00 0.00907 18400.00 10000.00 0.00 0.01030 18600.00 10000.00 0.00 0.01140 18800.00 10000.00 0.00 0.01242 19000.00 10000.00 0.00 0.01351 19200.00 10000.00 0.00 0.01480 19400.00 10000.00 0.00 0.01611 19600.00 10000.00 0.00 0.01749 19800.00 10000.00 0.00 0.01905 20000.00 10000.00 0.00 0.02077 20200.00 10000.00 0.00 0.02247 20400.00 10000.00 0.00 0.02399 20600.00 10000.00 0.00 0.02564 20800.00 10000.00 0.00 0.02754 21000.00 10000.00 0.00 0.02955 21200.00 10000.00 0.00 0.03165 21400.00 10000.00 0.00 0.03375 21600.00 10000.00 0.00 0.03586 21800.00 10000.00 0.00 0.03770 22000.00 10000.00 0.00 0.03889 22200.00 10000.00 0.00 0.03945 22400.00 10000.00 0.00 0.03971 22600.00 10000.00 0.00 0.03976 22200.00 10200.00 0.00 0.04000 22400.00 10200.00 0.00 0.04027 22600.00 10200.00 0.00 0.04031 22200.00 10400.00 0.00 0.04068 22400.00 10400.00 0.00 0.04093 22200.00 10600.00 0.00 0.04123 22400.00 10600.00 0.00 0.04143 22200.00 10800.00 0.00 0.04160 22400.00 10800.00 0.00 0.04176 22200.00 11000.00 0.00 0.04220 22400.00 11000.00 0.00 0.04236 22200.00 11200.00 0.00 0.04276 22400.00 11200.00 0.00 0.04293 22200.00 11400.00 0.00 0.04329 22400.00 11400.00 0.00 0.04347 22200.00 11600.00 0.00 0.04389 22400.00 11600.00 0.00 0.04407 22200.00 11800.00 0.00 0.04447 22400.00 11800.00 0.00 0.04464 22000.00 12000.00 0.00 0.04471 22200.00 12000.00 0.00 0.04517 22400.00 12000.00 0.00 0.04533 22000.00 12200.00 0.00 0.04545 22200.00 12200.00 0.00 0.04589 22400.00 12200.00 0.00 0.04603 22000.00 12400.00 0.00 0.04616 22200.00 12400.00 0.00 0.04660 22400.00 12400.00 0.00 0.04674 22000.00 12600.00 0.00 0.04686 22200.00 12600.00 0.00 0.04736 22400.00 12600.00 0.00 0.04751 22000.00 12800.00 0.00 0.04780 22200.00 12800.00 0.00 0.04835 22400.00 12800.00 0.00 0.04849 22000.00 13000.00 0.00 0.04895 22200.00 13000.00 0.00 0.04949 22400.00 13000.00 0.00 0.04959 22000.00 13200.00 0.00 0.05014 22200.00 13200.00 0.00 0.05064 22400.00 13200.00 0.00 0.05071 22000.00 13400.00 0.00 0.05137 22200.00 13400.00 0.00 0.05182 22400.00 13400.00 0.00 0.05188 22000.00 13600.00 0.00 0.05242 22200.00 13600.00 0.00 0.05283 22400.00 13600.00 0.00 0.05291 22000.00 13800.00 0.00 0.05334 22200.00 13800.00 0.00 0.05377 22400.00 13800.00 0.00 0.05388 22000.00 14000.00 0.00 0.05447 22200.00 14000.00 0.00 0.05492 22400.00 14000.00 0.00 0.05500 22000.00 14200.00 0.00 0.05577 22200.00 14200.00 0.00 0.05616 22400.00 14200.00 0.00 0.05619 22000.00 14400.00 0.00 0.05702 22200.00 14400.00 0.00 0.05734 22400.00 14400.00 0.00 0.05735 22000.00 14600.00 0.00 0.05817 22200.00 14600.00 0.00 0.05850 22400.00 14600.00 0.00 0.05855 22000.00 14800.00 0.00 0.05937 22200.00 14800.00 0.00 0.05976 22400.00 14800.00 0.00 0.05987 22000.00 15000.00 0.00 0.06083 22200.00 15000.00 0.00 0.06123 22400.00 15000.00 0.00 0.06133 22000.00 15200.00 0.00 0.06226 22200.00 15200.00 0.00 0.06269 22400.00 15200.00 0.00 0.06277 22000.00 15400.00 0.00 0.06379 22200.00 15400.00 0.00 0.06425 22400.00 15400.00 0.00 0.06431 22000.00 15600.00 0.00 0.06538 22200.00 15600.00 0.00 0.06581 22000.00 15800.00 0.00 0.06671 22200.00 15800.00 0.00 0.06710 22000.00 16000.00 0.00 0.06810 22200.00 16000.00 0.00 0.06854 22000.00 16200.00 0.00 0.06962 22200.00 16200.00 0.00 0.07002 22000.00 16400.00 0.00 0.07117 22200.00 16400.00 0.00 0.07146 21800.00 16600.00 0.00 0.07151 22000.00 16600.00 0.00 0.07277 22200.00 16600.00 0.00 0.07299 21800.00 16800.00 0.00 0.07330 22000.00 16800.00 0.00 0.07456 22200.00 16800.00 0.00 0.07471 21800.00 17000.00 0.00 0.07514 22000.00 17000.00 0.00 0.07634 22200.00 17000.00 0.00 0.07640 21800.00 17200.00 0.00 0.07682 22000.00 17200.00 0.00 0.07795 22200.00 17200.00 0.00 0.07795 21800.00 17400.00 0.00 0.07862 22000.00 17400.00 0.00 0.07969 21800.00 17600.00 0.00 0.08039 22000.00 17600.00 0.00 0.08141 21800.00 17800.00 0.00 0.08218 22000.00 17800.00 0.00 0.08314 21800.00 18000.00 0.00 0.08402 22000.00 18000.00 0.00 0.08494 21800.00 18200.00 0.00 0.08589 22000.00 18200.00 0.00 0.08674 21800.00 18400.00 0.00 0.08764 22000.00 18400.00 0.00 0.08840 21800.00 18600.00 0.00 0.08934 22000.00 18600.00 0.00 0.09005 21800.00 18800.00 0.00 0.09116 22000.00 18800.00 0.00 0.09185 21800.00 19000.00 0.00 0.09295 22000.00 19000.00 0.00 0.09362 21800.00 19200.00 0.00 0.09499 22000.00 19200.00 0.00 0.09561 21800.00 19400.00 0.00 0.09712 22000.00 19400.00 0.00 0.09764 21800.00 19600.00 0.00 0.09928 22000.00 19600.00 0.00 0.09969 21800.00 19800.00 0.00 0.10134 22000.00 19800.00 0.00 0.10172 21800.00 20000.00 0.00 0.10352 22000.00 20000.00 0.00 0.10382 21600.00 20200.00 0.00 0.10399 21800.00 20200.00 0.00 0.10577 22000.00 20200.00 0.00 0.10591 21600.00 20400.00 0.00 0.10597 21800.00 20400.00 0.00 0.10764 21600.00 20600.00 0.00 0.10794 21800.00 20600.00 0.00 0.10950 21600.00 20800.00 0.00 0.10985 21800.00 20800.00 0.00 0.11131 21600.00 21000.00 0.00 0.11188 21800.00 21000.00 0.00 0.11319 21600.00 21200.00 0.00 0.11380 21800.00 21200.00 0.00 0.11493 21600.00 21400.00 0.00 0.11593 21800.00 21400.00 0.00 0.11673 21600.00 21600.00 0.00 0.11790 21800.00 21600.00 0.00 0.11801 21600.00 21800.00 0.00 0.11824 21600.00 21800.00 5.00 0.11829 21600.00 21800.00 10.00 0.11833 21600.00 21800.00 15.00 0.11837 21600.00 21800.00 20.00 0.11840 21600.00 21800.00 25.00 0.11841 21600.00 21800.00 30.00 0.11842 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21646.79 21722.13 34.82 0.11852 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/593.ctf NX, NY, NZ: 512 512 1 MODE : real