CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/590.mrc Output diagnostic file name Micrographs_0601/590.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/590.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2410.000 8170.000 Mean, RMS : 4528.592 216.9630 TITLE 1: emi2mrc: 590.emi Mon Jan 06 19:19:08 2014 TITLE 2: kV 300, Mag 78000x, Def 25270, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -24.670, Y 211.985, Z -42.241, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01681 10200.00 10000.00 0.00 0.01814 10400.00 10000.00 0.00 0.01931 10600.00 10000.00 0.00 0.02061 10800.00 10000.00 0.00 0.02202 11000.00 10000.00 0.00 0.02354 11200.00 10000.00 0.00 0.02514 11400.00 10000.00 0.00 0.02682 11600.00 10000.00 0.00 0.02874 11800.00 10000.00 0.00 0.03087 12000.00 10000.00 0.00 0.03307 12200.00 10000.00 0.00 0.03521 12400.00 10000.00 0.00 0.03768 12600.00 10000.00 0.00 0.04030 12800.00 10000.00 0.00 0.04290 13000.00 10000.00 0.00 0.04548 13200.00 10000.00 0.00 0.04815 13400.00 10000.00 0.00 0.05102 13600.00 10000.00 0.00 0.05384 13800.00 10000.00 0.00 0.05674 14000.00 10000.00 0.00 0.05996 14200.00 10000.00 0.00 0.06356 14400.00 10000.00 0.00 0.06692 14600.00 10000.00 0.00 0.07026 14800.00 10000.00 0.00 0.07379 15000.00 10000.00 0.00 0.07629 15200.00 10000.00 0.00 0.07750 15400.00 10000.00 0.00 0.07790 15600.00 10000.00 0.00 0.07811 15200.00 10200.00 0.00 0.07934 15400.00 10200.00 0.00 0.07974 15600.00 10200.00 0.00 0.07988 15200.00 10400.00 0.00 0.08111 15400.00 10400.00 0.00 0.08142 15600.00 10400.00 0.00 0.08144 15000.00 10600.00 0.00 0.08181 15200.00 10600.00 0.00 0.08298 15400.00 10600.00 0.00 0.08322 15000.00 10800.00 0.00 0.08391 15200.00 10800.00 0.00 0.08502 15400.00 10800.00 0.00 0.08521 15000.00 11000.00 0.00 0.08626 15200.00 11000.00 0.00 0.08733 15400.00 11000.00 0.00 0.08747 15000.00 11200.00 0.00 0.08860 15200.00 11200.00 0.00 0.08964 15400.00 11200.00 0.00 0.08970 15000.00 11400.00 0.00 0.09124 15200.00 11400.00 0.00 0.09221 15000.00 11600.00 0.00 0.09382 15200.00 11600.00 0.00 0.09470 15000.00 11800.00 0.00 0.09649 15200.00 11800.00 0.00 0.09731 15000.00 12000.00 0.00 0.09938 15200.00 12000.00 0.00 0.10017 15000.00 12200.00 0.00 0.10243 15200.00 12200.00 0.00 0.10314 14800.00 12400.00 0.00 0.10324 15000.00 12400.00 0.00 0.10562 15200.00 12400.00 0.00 0.10618 14800.00 12600.00 0.00 0.10637 15000.00 12600.00 0.00 0.10868 15200.00 12600.00 0.00 0.10915 14800.00 12800.00 0.00 0.10984 15000.00 12800.00 0.00 0.11207 15200.00 12800.00 0.00 0.11248 14800.00 13000.00 0.00 0.11307 15000.00 13000.00 0.00 0.11523 15200.00 13000.00 0.00 0.11557 14800.00 13200.00 0.00 0.11643 15000.00 13200.00 0.00 0.11859 15200.00 13200.00 0.00 0.11886 14800.00 13400.00 0.00 0.12011 15000.00 13400.00 0.00 0.12227 15200.00 13400.00 0.00 0.12239 14800.00 13600.00 0.00 0.12393 15000.00 13600.00 0.00 0.12601 14800.00 13800.00 0.00 0.12785 15000.00 13800.00 0.00 0.12975 14800.00 14000.00 0.00 0.13173 15000.00 14000.00 0.00 0.13339 14800.00 14200.00 0.00 0.13615 15000.00 14200.00 0.00 0.13745 14800.00 14400.00 0.00 0.14022 15000.00 14400.00 0.00 0.14108 14800.00 14600.00 0.00 0.14477 15000.00 14600.00 0.00 0.14480 14600.00 14800.00 0.00 0.14530 14800.00 14800.00 0.00 0.14834 14800.00 15000.00 0.00 0.14879 14800.00 15000.00 5.00 0.14888 14800.00 15000.00 10.00 0.14895 14800.00 15000.00 15.00 0.14899 14800.00 15000.00 20.00 0.14900 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14784.67 14964.31 18.36 0.14906 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/590.ctf NX, NY, NZ: 512 512 1 MODE : real