CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/587.mrc Output diagnostic file name Micrographs_0601/587.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/587.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2216.000 7742.000 Mean, RMS : 4537.060 218.8001 TITLE 1: emi2mrc: 587.emi Mon Jan 06 19:17:10 2014 TITLE 2: kV 300, Mag 78000x, Def 25270, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -30.796, Y 220.538, Z -42.241, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01743 12000.00 10000.00 0.00 -0.01730 12200.00 10000.00 0.00 -0.01703 12400.00 10000.00 0.00 -0.01677 12600.00 10000.00 0.00 -0.01647 12800.00 10000.00 0.00 -0.01612 13000.00 10000.00 0.00 -0.01571 13200.00 10000.00 0.00 -0.01534 13400.00 10000.00 0.00 -0.01503 13600.00 10000.00 0.00 -0.01455 13800.00 10000.00 0.00 -0.01397 14000.00 10000.00 0.00 -0.01339 14200.00 10000.00 0.00 -0.01295 14400.00 10000.00 0.00 -0.01267 14600.00 10000.00 0.00 -0.01226 14800.00 10000.00 0.00 -0.01160 15000.00 10000.00 0.00 -0.01081 15200.00 10000.00 0.00 -0.01001 15400.00 10000.00 0.00 -0.00913 15600.00 10000.00 0.00 -0.00814 15800.00 10000.00 0.00 -0.00729 16000.00 10000.00 0.00 -0.00642 16200.00 10000.00 0.00 -0.00540 16400.00 10000.00 0.00 -0.00439 16600.00 10000.00 0.00 -0.00340 16800.00 10000.00 0.00 -0.00218 17000.00 10000.00 0.00 -0.00084 17200.00 10000.00 0.00 0.00030 17400.00 10000.00 0.00 0.00147 17600.00 10000.00 0.00 0.00276 17800.00 10000.00 0.00 0.00400 18000.00 10000.00 0.00 0.00529 18200.00 10000.00 0.00 0.00649 18400.00 10000.00 0.00 0.00756 18600.00 10000.00 0.00 0.00862 18800.00 10000.00 0.00 0.00970 19000.00 10000.00 0.00 0.01082 19200.00 10000.00 0.00 0.01198 19400.00 10000.00 0.00 0.01305 19600.00 10000.00 0.00 0.01428 19800.00 10000.00 0.00 0.01581 20000.00 10000.00 0.00 0.01730 20200.00 10000.00 0.00 0.01879 20400.00 10000.00 0.00 0.02038 20600.00 10000.00 0.00 0.02201 20800.00 10000.00 0.00 0.02374 21000.00 10000.00 0.00 0.02550 21200.00 10000.00 0.00 0.02736 21400.00 10000.00 0.00 0.02928 21600.00 10000.00 0.00 0.03116 21800.00 10000.00 0.00 0.03296 22000.00 10000.00 0.00 0.03462 22200.00 10000.00 0.00 0.03585 22400.00 10000.00 0.00 0.03653 22600.00 10000.00 0.00 0.03704 22800.00 10000.00 0.00 0.03747 23000.00 10000.00 0.00 0.03764 23200.00 10000.00 0.00 0.03767 22800.00 10200.00 0.00 0.03790 23000.00 10200.00 0.00 0.03805 23200.00 10200.00 0.00 0.03810 22800.00 10400.00 0.00 0.03848 23000.00 10400.00 0.00 0.03861 23200.00 10400.00 0.00 0.03865 22800.00 10600.00 0.00 0.03896 23000.00 10600.00 0.00 0.03909 23200.00 10600.00 0.00 0.03912 22600.00 10800.00 0.00 0.03913 22800.00 10800.00 0.00 0.03949 23000.00 10800.00 0.00 0.03964 23200.00 10800.00 0.00 0.03966 22600.00 11000.00 0.00 0.03974 22800.00 11000.00 0.00 0.04010 23000.00 11000.00 0.00 0.04024 22600.00 11200.00 0.00 0.04032 22800.00 11200.00 0.00 0.04064 23000.00 11200.00 0.00 0.04072 22600.00 11400.00 0.00 0.04098 22800.00 11400.00 0.00 0.04128 23000.00 11400.00 0.00 0.04137 22600.00 11600.00 0.00 0.04171 22800.00 11600.00 0.00 0.04204 23000.00 11600.00 0.00 0.04216 22600.00 11800.00 0.00 0.04249 22800.00 11800.00 0.00 0.04284 23000.00 11800.00 0.00 0.04296 22600.00 12000.00 0.00 0.04340 22800.00 12000.00 0.00 0.04374 23000.00 12000.00 0.00 0.04382 22400.00 12200.00 0.00 0.04396 22600.00 12200.00 0.00 0.04444 22800.00 12200.00 0.00 0.04475 23000.00 12200.00 0.00 0.04478 22400.00 12400.00 0.00 0.04484 22600.00 12400.00 0.00 0.04529 22800.00 12400.00 0.00 0.04556 23000.00 12400.00 0.00 0.04558 22400.00 12600.00 0.00 0.04565 22600.00 12600.00 0.00 0.04609 22800.00 12600.00 0.00 0.04638 23000.00 12600.00 0.00 0.04643 22400.00 12800.00 0.00 0.04667 22600.00 12800.00 0.00 0.04713 22800.00 12800.00 0.00 0.04745 23000.00 12800.00 0.00 0.04754 22400.00 13000.00 0.00 0.04768 22600.00 13000.00 0.00 0.04818 22800.00 13000.00 0.00 0.04853 23000.00 13000.00 0.00 0.04862 22400.00 13200.00 0.00 0.04885 22600.00 13200.00 0.00 0.04937 22800.00 13200.00 0.00 0.04974 23000.00 13200.00 0.00 0.04981 22400.00 13400.00 0.00 0.05001 22600.00 13400.00 0.00 0.05055 22800.00 13400.00 0.00 0.05089 23000.00 13400.00 0.00 0.05092 22400.00 13600.00 0.00 0.05104 22600.00 13600.00 0.00 0.05157 22800.00 13600.00 0.00 0.05187 22400.00 13800.00 0.00 0.05203 22600.00 13800.00 0.00 0.05257 22800.00 13800.00 0.00 0.05286 22400.00 14000.00 0.00 0.05309 22600.00 14000.00 0.00 0.05366 22800.00 14000.00 0.00 0.05393 22400.00 14200.00 0.00 0.05428 22600.00 14200.00 0.00 0.05486 22800.00 14200.00 0.00 0.05509 22400.00 14400.00 0.00 0.05550 22600.00 14400.00 0.00 0.05603 22800.00 14400.00 0.00 0.05616 22400.00 14600.00 0.00 0.05662 22600.00 14600.00 0.00 0.05708 22800.00 14600.00 0.00 0.05713 22400.00 14800.00 0.00 0.05754 22600.00 14800.00 0.00 0.05796 22400.00 15000.00 0.00 0.05849 22600.00 15000.00 0.00 0.05885 22400.00 15200.00 0.00 0.05935 22600.00 15200.00 0.00 0.05964 22400.00 15400.00 0.00 0.06019 22600.00 15400.00 0.00 0.06044 22200.00 15600.00 0.00 0.06057 22400.00 15600.00 0.00 0.06118 22600.00 15600.00 0.00 0.06142 22200.00 15800.00 0.00 0.06160 22400.00 15800.00 0.00 0.06221 22600.00 15800.00 0.00 0.06248 22200.00 16000.00 0.00 0.06279 22400.00 16000.00 0.00 0.06345 22600.00 16000.00 0.00 0.06376 22200.00 16200.00 0.00 0.06408 22400.00 16200.00 0.00 0.06478 22600.00 16200.00 0.00 0.06510 22200.00 16400.00 0.00 0.06562 22400.00 16400.00 0.00 0.06632 22600.00 16400.00 0.00 0.06659 22200.00 16600.00 0.00 0.06720 22400.00 16600.00 0.00 0.06790 22600.00 16600.00 0.00 0.06815 22200.00 16800.00 0.00 0.06887 22400.00 16800.00 0.00 0.06960 22600.00 16800.00 0.00 0.06989 22200.00 17000.00 0.00 0.07068 22400.00 17000.00 0.00 0.07143 22600.00 17000.00 0.00 0.07170 22200.00 17200.00 0.00 0.07233 22400.00 17200.00 0.00 0.07302 22600.00 17200.00 0.00 0.07324 22200.00 17400.00 0.00 0.07386 22400.00 17400.00 0.00 0.07451 22600.00 17400.00 0.00 0.07470 22200.00 17600.00 0.00 0.07530 22400.00 17600.00 0.00 0.07593 22600.00 17600.00 0.00 0.07609 22200.00 17800.00 0.00 0.07692 22400.00 17800.00 0.00 0.07754 22600.00 17800.00 0.00 0.07766 22200.00 18000.00 0.00 0.07857 22400.00 18000.00 0.00 0.07914 22600.00 18000.00 0.00 0.07920 22200.00 18200.00 0.00 0.08021 22400.00 18200.00 0.00 0.08074 22600.00 18200.00 0.00 0.08075 22200.00 18400.00 0.00 0.08199 22400.00 18400.00 0.00 0.08250 22000.00 18600.00 0.00 0.08252 22200.00 18600.00 0.00 0.08388 22400.00 18600.00 0.00 0.08434 22000.00 18800.00 0.00 0.08446 22200.00 18800.00 0.00 0.08575 22400.00 18800.00 0.00 0.08609 22000.00 19000.00 0.00 0.08623 22200.00 19000.00 0.00 0.08743 22400.00 19000.00 0.00 0.08767 22000.00 19200.00 0.00 0.08820 22200.00 19200.00 0.00 0.08934 22400.00 19200.00 0.00 0.08951 22000.00 19400.00 0.00 0.09006 22200.00 19400.00 0.00 0.09117 22400.00 19400.00 0.00 0.09127 22000.00 19600.00 0.00 0.09197 22200.00 19600.00 0.00 0.09304 22400.00 19600.00 0.00 0.09308 22000.00 19800.00 0.00 0.09399 22200.00 19800.00 0.00 0.09502 22000.00 20000.00 0.00 0.09606 22200.00 20000.00 0.00 0.09701 22000.00 20200.00 0.00 0.09822 22200.00 20200.00 0.00 0.09907 22000.00 20400.00 0.00 0.10014 22200.00 20400.00 0.00 0.10090 22000.00 20600.00 0.00 0.10211 22200.00 20600.00 0.00 0.10280 22000.00 20800.00 0.00 0.10400 22200.00 20800.00 0.00 0.10459 22000.00 21000.00 0.00 0.10598 22200.00 21000.00 0.00 0.10640 22000.00 21200.00 0.00 0.10794 22200.00 21200.00 0.00 0.10813 21800.00 21400.00 0.00 0.10822 22000.00 21400.00 0.00 0.10979 21800.00 21600.00 0.00 0.11006 22000.00 21600.00 0.00 0.11146 21800.00 21800.00 0.00 0.11147 22000.00 21800.00 0.00 0.11272 22000.00 22000.00 0.00 0.11355 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21945.33 22075.65 33.47 0.11371 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/587.ctf NX, NY, NZ: 512 512 1 MODE : real