CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/585.mrc Output diagnostic file name Micrographs_0601/585.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/585.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2466.000 7848.000 Mean, RMS : 4581.153 222.0230 TITLE 1: emi2mrc: 585.emi Mon Jan 06 19:15:23 2014 TITLE 2: kV 300, Mag 78000x, Def 23460, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X -33.955, Y 224.742, Z -40.486, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01192 11800.00 10000.00 0.00 -0.01189 12000.00 10000.00 0.00 -0.01178 12200.00 10000.00 0.00 -0.01162 12400.00 10000.00 0.00 -0.01147 12600.00 10000.00 0.00 -0.01139 12800.00 10000.00 0.00 -0.01123 13000.00 10000.00 0.00 -0.01099 13200.00 10000.00 0.00 -0.01071 13400.00 10000.00 0.00 -0.01035 13600.00 10000.00 0.00 -0.00997 13800.00 10000.00 0.00 -0.00970 14000.00 10000.00 0.00 -0.00937 14200.00 10000.00 0.00 -0.00892 14400.00 10000.00 0.00 -0.00832 14600.00 10000.00 0.00 -0.00757 14800.00 10000.00 0.00 -0.00689 15000.00 10000.00 0.00 -0.00635 15200.00 10000.00 0.00 -0.00576 15400.00 10000.00 0.00 -0.00490 15600.00 10000.00 0.00 -0.00415 15800.00 10000.00 0.00 -0.00363 16000.00 10000.00 0.00 -0.00291 16200.00 10000.00 0.00 -0.00207 16400.00 10000.00 0.00 -0.00126 16600.00 10000.00 0.00 -0.00032 16800.00 10000.00 0.00 0.00052 17000.00 10000.00 0.00 0.00145 17200.00 10000.00 0.00 0.00251 17400.00 10000.00 0.00 0.00350 17600.00 10000.00 0.00 0.00447 17800.00 10000.00 0.00 0.00540 18000.00 10000.00 0.00 0.00652 18200.00 10000.00 0.00 0.00769 18400.00 10000.00 0.00 0.00883 18600.00 10000.00 0.00 0.01000 18800.00 10000.00 0.00 0.01114 19000.00 10000.00 0.00 0.01239 19200.00 10000.00 0.00 0.01361 19400.00 10000.00 0.00 0.01488 19600.00 10000.00 0.00 0.01622 19800.00 10000.00 0.00 0.01753 20000.00 10000.00 0.00 0.01895 20200.00 10000.00 0.00 0.02045 20400.00 10000.00 0.00 0.02201 20600.00 10000.00 0.00 0.02360 20800.00 10000.00 0.00 0.02528 21000.00 10000.00 0.00 0.02718 21200.00 10000.00 0.00 0.02916 21400.00 10000.00 0.00 0.03108 21600.00 10000.00 0.00 0.03293 21800.00 10000.00 0.00 0.03484 22000.00 10000.00 0.00 0.03662 22200.00 10000.00 0.00 0.03768 22400.00 10000.00 0.00 0.03817 22600.00 10000.00 0.00 0.03850 22800.00 10000.00 0.00 0.03877 23000.00 10000.00 0.00 0.03881 22600.00 10200.00 0.00 0.03907 22800.00 10200.00 0.00 0.03937 23000.00 10200.00 0.00 0.03942 22600.00 10400.00 0.00 0.03977 22800.00 10400.00 0.00 0.04012 23000.00 10400.00 0.00 0.04014 22400.00 10600.00 0.00 0.04018 22600.00 10600.00 0.00 0.04067 22800.00 10600.00 0.00 0.04098 22400.00 10800.00 0.00 0.04098 22600.00 10800.00 0.00 0.04144 22800.00 10800.00 0.00 0.04167 22400.00 11000.00 0.00 0.04179 22600.00 11000.00 0.00 0.04217 22800.00 11000.00 0.00 0.04235 22400.00 11200.00 0.00 0.04251 22600.00 11200.00 0.00 0.04286 22800.00 11200.00 0.00 0.04301 22400.00 11400.00 0.00 0.04324 22600.00 11400.00 0.00 0.04356 22800.00 11400.00 0.00 0.04368 22400.00 11600.00 0.00 0.04404 22600.00 11600.00 0.00 0.04431 22800.00 11600.00 0.00 0.04440 22400.00 11800.00 0.00 0.04478 22600.00 11800.00 0.00 0.04501 22800.00 11800.00 0.00 0.04509 22400.00 12000.00 0.00 0.04542 22600.00 12000.00 0.00 0.04566 22800.00 12000.00 0.00 0.04576 22400.00 12200.00 0.00 0.04615 22600.00 12200.00 0.00 0.04642 22800.00 12200.00 0.00 0.04653 22400.00 12400.00 0.00 0.04699 22600.00 12400.00 0.00 0.04725 22800.00 12400.00 0.00 0.04734 22400.00 12600.00 0.00 0.04780 22600.00 12600.00 0.00 0.04803 22800.00 12600.00 0.00 0.04807 22200.00 12800.00 0.00 0.04814 22400.00 12800.00 0.00 0.04858 22600.00 12800.00 0.00 0.04872 22200.00 13000.00 0.00 0.04884 22400.00 13000.00 0.00 0.04921 22600.00 13000.00 0.00 0.04928 22200.00 13200.00 0.00 0.04961 22400.00 13200.00 0.00 0.04989 22600.00 13200.00 0.00 0.04992 22200.00 13400.00 0.00 0.05043 22400.00 13400.00 0.00 0.05064 22600.00 13400.00 0.00 0.05066 22200.00 13600.00 0.00 0.05121 22400.00 13600.00 0.00 0.05139 22600.00 13600.00 0.00 0.05141 22200.00 13800.00 0.00 0.05197 22400.00 13800.00 0.00 0.05212 22600.00 13800.00 0.00 0.05214 22200.00 14000.00 0.00 0.05275 22400.00 14000.00 0.00 0.05286 22600.00 14000.00 0.00 0.05288 22200.00 14200.00 0.00 0.05355 22400.00 14200.00 0.00 0.05365 22600.00 14200.00 0.00 0.05370 22200.00 14400.00 0.00 0.05441 22400.00 14400.00 0.00 0.05453 22600.00 14400.00 0.00 0.05461 22800.00 14400.00 0.00 0.05461 22000.00 14600.00 0.00 0.05470 22200.00 14600.00 0.00 0.05547 22400.00 14600.00 0.00 0.05559 22600.00 14600.00 0.00 0.05568 22800.00 14600.00 0.00 0.05569 22200.00 14800.00 0.00 0.05633 22400.00 14800.00 0.00 0.05646 22600.00 14800.00 0.00 0.05659 22800.00 14800.00 0.00 0.05664 22200.00 15000.00 0.00 0.05718 22400.00 15000.00 0.00 0.05737 22600.00 15000.00 0.00 0.05758 22800.00 15000.00 0.00 0.05765 22200.00 15200.00 0.00 0.05837 22400.00 15200.00 0.00 0.05864 22600.00 15200.00 0.00 0.05889 22800.00 15200.00 0.00 0.05892 22200.00 15400.00 0.00 0.05978 22400.00 15400.00 0.00 0.06005 22600.00 15400.00 0.00 0.06027 22000.00 15600.00 0.00 0.06035 22200.00 15600.00 0.00 0.06122 22400.00 15600.00 0.00 0.06148 22600.00 15600.00 0.00 0.06169 22000.00 15800.00 0.00 0.06173 22200.00 15800.00 0.00 0.06261 22400.00 15800.00 0.00 0.06288 22600.00 15800.00 0.00 0.06310 22000.00 16000.00 0.00 0.06313 22200.00 16000.00 0.00 0.06400 22400.00 16000.00 0.00 0.06426 22600.00 16000.00 0.00 0.06446 22200.00 16200.00 0.00 0.06520 22400.00 16200.00 0.00 0.06546 22600.00 16200.00 0.00 0.06566 22200.00 16400.00 0.00 0.06652 22400.00 16400.00 0.00 0.06683 22600.00 16400.00 0.00 0.06705 22000.00 16600.00 0.00 0.06721 22200.00 16600.00 0.00 0.06815 22400.00 16600.00 0.00 0.06848 22600.00 16600.00 0.00 0.06868 22000.00 16800.00 0.00 0.06891 22200.00 16800.00 0.00 0.06985 22400.00 16800.00 0.00 0.07016 22600.00 16800.00 0.00 0.07034 22000.00 17000.00 0.00 0.07062 22200.00 17000.00 0.00 0.07156 22400.00 17000.00 0.00 0.07188 22600.00 17000.00 0.00 0.07206 22000.00 17200.00 0.00 0.07246 22200.00 17200.00 0.00 0.07340 22400.00 17200.00 0.00 0.07372 22600.00 17200.00 0.00 0.07390 22000.00 17400.00 0.00 0.07440 22200.00 17400.00 0.00 0.07533 22400.00 17400.00 0.00 0.07566 22600.00 17400.00 0.00 0.07580 22000.00 17600.00 0.00 0.07631 22200.00 17600.00 0.00 0.07728 22400.00 17600.00 0.00 0.07762 22600.00 17600.00 0.00 0.07773 22000.00 17800.00 0.00 0.07833 22200.00 17800.00 0.00 0.07935 22400.00 17800.00 0.00 0.07968 22600.00 17800.00 0.00 0.07971 22000.00 18000.00 0.00 0.08027 22200.00 18000.00 0.00 0.08130 22400.00 18000.00 0.00 0.08155 22000.00 18200.00 0.00 0.08224 22200.00 18200.00 0.00 0.08323 22400.00 18200.00 0.00 0.08338 22000.00 18400.00 0.00 0.08407 22200.00 18400.00 0.00 0.08501 22400.00 18400.00 0.00 0.08507 22000.00 18600.00 0.00 0.08590 22200.00 18600.00 0.00 0.08679 22400.00 18600.00 0.00 0.08679 22000.00 18800.00 0.00 0.08803 22200.00 18800.00 0.00 0.08887 22000.00 19000.00 0.00 0.09004 22200.00 19000.00 0.00 0.09079 22000.00 19200.00 0.00 0.09209 22200.00 19200.00 0.00 0.09278 22000.00 19400.00 0.00 0.09415 22200.00 19400.00 0.00 0.09481 22000.00 19600.00 0.00 0.09650 22200.00 19600.00 0.00 0.09715 22000.00 19800.00 0.00 0.09903 22200.00 19800.00 0.00 0.09960 22000.00 20000.00 0.00 0.10147 22200.00 20000.00 0.00 0.10197 22000.00 20200.00 0.00 0.10417 22200.00 20200.00 0.00 0.10460 21800.00 20400.00 0.00 0.10479 22000.00 20400.00 0.00 0.10712 22200.00 20400.00 0.00 0.10742 21800.00 20600.00 0.00 0.10807 22000.00 20600.00 0.00 0.11035 22200.00 20600.00 0.00 0.11039 21800.00 20800.00 0.00 0.11128 22000.00 20800.00 0.00 0.11341 21800.00 21000.00 0.00 0.11471 22000.00 21000.00 0.00 0.11664 21800.00 21200.00 0.00 0.11832 22000.00 21200.00 0.00 0.12006 21800.00 21400.00 0.00 0.12202 22000.00 21400.00 0.00 0.12345 21800.00 21600.00 0.00 0.12569 22000.00 21600.00 0.00 0.12660 21800.00 21800.00 0.00 0.12842 22000.00 21800.00 0.00 0.12854 21800.00 22000.00 5.00 0.12868 21800.00 22000.00 10.00 0.12883 21800.00 22000.00 15.00 0.12897 21800.00 22000.00 20.00 0.12911 21800.00 22000.00 25.00 0.12923 21800.00 22000.00 30.00 0.12933 21800.00 22000.00 35.00 0.12940 21800.00 22000.00 40.00 0.12945 21800.00 22000.00 45.00 0.12947 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21808.73 21956.28 45.00 0.12956 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/585.ctf NX, NY, NZ: 512 512 1 MODE : real