CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/584.mrc Output diagnostic file name Micrographs_0601/584.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/584.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2454.000 9912.000 Mean, RMS : 4577.931 222.2666 TITLE 1: emi2mrc: 584.emi Mon Jan 06 19:14:49 2014 TITLE 2: kV 300, Mag 78000x, Def 23460, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X -35.429, Y 226.857, Z -40.486, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01329 10200.00 10000.00 0.00 -0.01329 10400.00 10000.00 0.00 -0.01314 10600.00 10000.00 0.00 -0.01291 10800.00 10000.00 0.00 -0.01262 11000.00 10000.00 0.00 -0.01244 11200.00 10000.00 0.00 -0.01227 11400.00 10000.00 0.00 -0.01208 11600.00 10000.00 0.00 -0.01180 11800.00 10000.00 0.00 -0.01155 12000.00 10000.00 0.00 -0.01125 12200.00 10000.00 0.00 -0.01078 12400.00 10000.00 0.00 -0.01063 13000.00 10000.00 0.00 -0.01037 13200.00 10000.00 0.00 -0.00989 13400.00 10000.00 0.00 -0.00933 13600.00 10000.00 0.00 -0.00877 13800.00 10000.00 0.00 -0.00831 14000.00 10000.00 0.00 -0.00791 14200.00 10000.00 0.00 -0.00749 14400.00 10000.00 0.00 -0.00705 14600.00 10000.00 0.00 -0.00648 14800.00 10000.00 0.00 -0.00583 15000.00 10000.00 0.00 -0.00521 15200.00 10000.00 0.00 -0.00447 15400.00 10000.00 0.00 -0.00375 15600.00 10000.00 0.00 -0.00300 15800.00 10000.00 0.00 -0.00206 16000.00 10000.00 0.00 -0.00107 16200.00 10000.00 0.00 0.00001 16400.00 10000.00 0.00 0.00100 16600.00 10000.00 0.00 0.00195 16800.00 10000.00 0.00 0.00300 17000.00 10000.00 0.00 0.00393 17200.00 10000.00 0.00 0.00472 17400.00 10000.00 0.00 0.00564 17600.00 10000.00 0.00 0.00657 17800.00 10000.00 0.00 0.00743 18000.00 10000.00 0.00 0.00846 18200.00 10000.00 0.00 0.00956 18400.00 10000.00 0.00 0.01072 18600.00 10000.00 0.00 0.01195 18800.00 10000.00 0.00 0.01299 19000.00 10000.00 0.00 0.01408 19200.00 10000.00 0.00 0.01530 19400.00 10000.00 0.00 0.01642 19600.00 10000.00 0.00 0.01758 19800.00 10000.00 0.00 0.01889 20000.00 10000.00 0.00 0.02031 20200.00 10000.00 0.00 0.02191 20400.00 10000.00 0.00 0.02363 20600.00 10000.00 0.00 0.02537 20800.00 10000.00 0.00 0.02721 21000.00 10000.00 0.00 0.02930 21200.00 10000.00 0.00 0.03142 21400.00 10000.00 0.00 0.03344 21600.00 10000.00 0.00 0.03558 21800.00 10000.00 0.00 0.03753 22000.00 10000.00 0.00 0.03878 22200.00 10000.00 0.00 0.03929 22400.00 10000.00 0.00 0.03951 22600.00 10000.00 0.00 0.03971 22200.00 10200.00 0.00 0.03990 22400.00 10200.00 0.00 0.04012 22600.00 10200.00 0.00 0.04028 22200.00 10400.00 0.00 0.04037 22400.00 10400.00 0.00 0.04063 22600.00 10400.00 0.00 0.04076 22200.00 10600.00 0.00 0.04099 22400.00 10600.00 0.00 0.04131 22600.00 10600.00 0.00 0.04143 22200.00 10800.00 0.00 0.04171 22400.00 10800.00 0.00 0.04204 22600.00 10800.00 0.00 0.04213 22200.00 11000.00 0.00 0.04238 22400.00 11000.00 0.00 0.04268 22600.00 11000.00 0.00 0.04270 22200.00 11200.00 0.00 0.04288 22400.00 11200.00 0.00 0.04314 22200.00 11400.00 0.00 0.04349 22400.00 11400.00 0.00 0.04373 22200.00 11600.00 0.00 0.04412 22400.00 11600.00 0.00 0.04439 22200.00 11800.00 0.00 0.04485 22400.00 11800.00 0.00 0.04512 22000.00 12000.00 0.00 0.04521 22200.00 12000.00 0.00 0.04577 22400.00 12000.00 0.00 0.04602 22000.00 12200.00 0.00 0.04611 22200.00 12200.00 0.00 0.04665 22400.00 12200.00 0.00 0.04684 22200.00 12400.00 0.00 0.04735 22400.00 12400.00 0.00 0.04754 22200.00 12600.00 0.00 0.04802 22400.00 12600.00 0.00 0.04822 22000.00 12800.00 0.00 0.04824 22200.00 12800.00 0.00 0.04876 22400.00 12800.00 0.00 0.04897 22000.00 13000.00 0.00 0.04903 22200.00 13000.00 0.00 0.04957 22400.00 13000.00 0.00 0.04980 22000.00 13200.00 0.00 0.05002 22200.00 13200.00 0.00 0.05059 22400.00 13200.00 0.00 0.05083 22000.00 13400.00 0.00 0.05104 22200.00 13400.00 0.00 0.05163 22400.00 13400.00 0.00 0.05183 22000.00 13600.00 0.00 0.05210 22200.00 13600.00 0.00 0.05267 22400.00 13600.00 0.00 0.05283 22000.00 13800.00 0.00 0.05318 22200.00 13800.00 0.00 0.05373 22400.00 13800.00 0.00 0.05387 22000.00 14000.00 0.00 0.05420 22200.00 14000.00 0.00 0.05475 22400.00 14000.00 0.00 0.05491 22000.00 14200.00 0.00 0.05526 22200.00 14200.00 0.00 0.05584 22400.00 14200.00 0.00 0.05600 22000.00 14400.00 0.00 0.05642 22200.00 14400.00 0.00 0.05700 22400.00 14400.00 0.00 0.05712 22000.00 14600.00 0.00 0.05774 22200.00 14600.00 0.00 0.05828 22400.00 14600.00 0.00 0.05838 22000.00 14800.00 0.00 0.05896 22200.00 14800.00 0.00 0.05949 22400.00 14800.00 0.00 0.05958 22000.00 15000.00 0.00 0.06021 22200.00 15000.00 0.00 0.06074 22400.00 15000.00 0.00 0.06082 22000.00 15200.00 0.00 0.06153 22200.00 15200.00 0.00 0.06209 22400.00 15200.00 0.00 0.06218 22000.00 15400.00 0.00 0.06299 22200.00 15400.00 0.00 0.06358 22400.00 15400.00 0.00 0.06369 22000.00 15600.00 0.00 0.06461 22200.00 15600.00 0.00 0.06518 22400.00 15600.00 0.00 0.06527 22000.00 15800.00 0.00 0.06619 22200.00 15800.00 0.00 0.06674 22400.00 15800.00 0.00 0.06680 22000.00 16000.00 0.00 0.06789 22200.00 16000.00 0.00 0.06844 22400.00 16000.00 0.00 0.06847 22000.00 16200.00 0.00 0.06959 22200.00 16200.00 0.00 0.07008 22000.00 16400.00 0.00 0.07132 22200.00 16400.00 0.00 0.07175 22000.00 16600.00 0.00 0.07288 22200.00 16600.00 0.00 0.07327 22000.00 16800.00 0.00 0.07444 22200.00 16800.00 0.00 0.07479 22000.00 17000.00 0.00 0.07606 22200.00 17000.00 0.00 0.07638 22000.00 17200.00 0.00 0.07762 22200.00 17200.00 0.00 0.07794 21800.00 17400.00 0.00 0.07814 22000.00 17400.00 0.00 0.07940 22200.00 17400.00 0.00 0.07968 21800.00 17600.00 0.00 0.07982 22000.00 17600.00 0.00 0.08104 22200.00 17600.00 0.00 0.08128 21800.00 17800.00 0.00 0.08161 22000.00 17800.00 0.00 0.08280 22200.00 17800.00 0.00 0.08302 21800.00 18000.00 0.00 0.08351 22000.00 18000.00 0.00 0.08468 22200.00 18000.00 0.00 0.08486 21800.00 18200.00 0.00 0.08540 22000.00 18200.00 0.00 0.08653 22200.00 18200.00 0.00 0.08666 21800.00 18400.00 0.00 0.08736 22000.00 18400.00 0.00 0.08849 22200.00 18400.00 0.00 0.08859 21800.00 18600.00 0.00 0.08947 22000.00 18600.00 0.00 0.09063 22200.00 18600.00 0.00 0.09069 21800.00 18800.00 0.00 0.09175 22000.00 18800.00 0.00 0.09291 21800.00 19000.00 0.00 0.09395 22000.00 19000.00 0.00 0.09510 21800.00 19200.00 0.00 0.09635 22000.00 19200.00 0.00 0.09742 21800.00 19400.00 0.00 0.09875 22000.00 19400.00 0.00 0.09970 21800.00 19600.00 0.00 0.10117 22000.00 19600.00 0.00 0.10202 21800.00 19800.00 0.00 0.10351 22000.00 19800.00 0.00 0.10428 21800.00 20000.00 0.00 0.10594 22000.00 20000.00 0.00 0.10663 21800.00 20200.00 0.00 0.10872 22000.00 20200.00 0.00 0.10931 21800.00 20400.00 0.00 0.11168 22000.00 20400.00 0.00 0.11210 21600.00 20600.00 0.00 0.11260 21800.00 20600.00 0.00 0.11498 22000.00 20600.00 0.00 0.11518 21600.00 20800.00 0.00 0.11606 21800.00 20800.00 0.00 0.11832 21600.00 21000.00 0.00 0.11980 21800.00 21000.00 0.00 0.12184 21600.00 21200.00 0.00 0.12333 21800.00 21200.00 0.00 0.12507 21600.00 21400.00 0.00 0.12700 21800.00 21400.00 0.00 0.12817 21600.00 21600.00 0.00 0.13012 21800.00 21600.00 0.00 0.13025 21600.00 21800.00 0.00 0.13026 21600.00 21800.00 5.00 0.13036 21600.00 21800.00 10.00 0.13047 21600.00 21800.00 15.00 0.13058 21600.00 21800.00 20.00 0.13067 21600.00 21800.00 25.00 0.13075 21600.00 21800.00 30.00 0.13082 21600.00 21800.00 35.00 0.13087 21600.00 21800.00 40.00 0.13090 21600.00 21800.00 45.00 0.13091 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21615.96 21737.85 45.01 0.13105 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/584.ctf NX, NY, NZ: 512 512 1 MODE : real