CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/582.mrc Output diagnostic file name Micrographs_0601/582.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/582.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2474.000 8500.000 Mean, RMS : 4578.583 222.5565 TITLE 1: emi2mrc: 582.emi Mon Jan 06 19:13:20 2014 TITLE 2: kV 300, Mag 78000x, Def 23460, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X -36.623, Y 233.072, Z -40.486, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01842 10400.00 10000.00 0.00 -0.01838 10600.00 10000.00 0.00 -0.01833 10800.00 10000.00 0.00 -0.01811 11000.00 10000.00 0.00 -0.01808 11400.00 10000.00 0.00 -0.01796 11600.00 10000.00 0.00 -0.01780 11800.00 10000.00 0.00 -0.01765 12000.00 10000.00 0.00 -0.01742 12200.00 10000.00 0.00 -0.01727 12400.00 10000.00 0.00 -0.01717 12600.00 10000.00 0.00 -0.01677 12800.00 10000.00 0.00 -0.01616 13000.00 10000.00 0.00 -0.01562 13200.00 10000.00 0.00 -0.01528 13400.00 10000.00 0.00 -0.01505 13600.00 10000.00 0.00 -0.01480 13800.00 10000.00 0.00 -0.01454 14000.00 10000.00 0.00 -0.01412 14200.00 10000.00 0.00 -0.01364 14400.00 10000.00 0.00 -0.01324 14600.00 10000.00 0.00 -0.01273 14800.00 10000.00 0.00 -0.01209 15000.00 10000.00 0.00 -0.01162 15200.00 10000.00 0.00 -0.01118 15400.00 10000.00 0.00 -0.01061 15600.00 10000.00 0.00 -0.01006 15800.00 10000.00 0.00 -0.00941 16000.00 10000.00 0.00 -0.00883 16200.00 10000.00 0.00 -0.00826 16400.00 10000.00 0.00 -0.00752 16600.00 10000.00 0.00 -0.00683 16800.00 10000.00 0.00 -0.00616 17000.00 10000.00 0.00 -0.00530 17200.00 10000.00 0.00 -0.00429 17400.00 10000.00 0.00 -0.00322 17600.00 10000.00 0.00 -0.00213 17800.00 10000.00 0.00 -0.00109 18000.00 10000.00 0.00 -0.00005 18200.00 10000.00 0.00 0.00099 18400.00 10000.00 0.00 0.00204 18600.00 10000.00 0.00 0.00311 18800.00 10000.00 0.00 0.00416 19000.00 10000.00 0.00 0.00516 19200.00 10000.00 0.00 0.00623 19400.00 10000.00 0.00 0.00738 19600.00 10000.00 0.00 0.00847 19800.00 10000.00 0.00 0.00963 20000.00 10000.00 0.00 0.01089 20200.00 10000.00 0.00 0.01210 20400.00 10000.00 0.00 0.01325 20600.00 10000.00 0.00 0.01436 20800.00 10000.00 0.00 0.01544 21000.00 10000.00 0.00 0.01665 21200.00 10000.00 0.00 0.01796 21400.00 10000.00 0.00 0.01912 21600.00 10000.00 0.00 0.02031 21800.00 10000.00 0.00 0.02172 22000.00 10000.00 0.00 0.02327 22200.00 10000.00 0.00 0.02499 22400.00 10000.00 0.00 0.02678 22600.00 10000.00 0.00 0.02861 22800.00 10000.00 0.00 0.03065 23000.00 10000.00 0.00 0.03262 23200.00 10000.00 0.00 0.03402 23400.00 10000.00 0.00 0.03474 23600.00 10000.00 0.00 0.03506 23800.00 10000.00 0.00 0.03521 24000.00 10000.00 0.00 0.03540 24200.00 10000.00 0.00 0.03559 23800.00 10200.00 0.00 0.03561 24000.00 10200.00 0.00 0.03583 24200.00 10200.00 0.00 0.03599 23800.00 10400.00 0.00 0.03611 24000.00 10400.00 0.00 0.03631 24200.00 10400.00 0.00 0.03644 23800.00 10600.00 0.00 0.03654 24000.00 10600.00 0.00 0.03672 24200.00 10600.00 0.00 0.03684 23800.00 10800.00 0.00 0.03688 24000.00 10800.00 0.00 0.03707 24200.00 10800.00 0.00 0.03719 23800.00 11000.00 0.00 0.03722 24000.00 11000.00 0.00 0.03739 24200.00 11000.00 0.00 0.03748 23800.00 11200.00 0.00 0.03759 24000.00 11200.00 0.00 0.03775 24200.00 11200.00 0.00 0.03784 23600.00 11400.00 0.00 0.03795 23800.00 11400.00 0.00 0.03811 24000.00 11400.00 0.00 0.03828 24200.00 11400.00 0.00 0.03839 23600.00 11600.00 0.00 0.03852 23800.00 11600.00 0.00 0.03869 24000.00 11600.00 0.00 0.03889 24200.00 11600.00 0.00 0.03902 24400.00 11600.00 0.00 0.03902 23600.00 11800.00 0.00 0.03918 23800.00 11800.00 0.00 0.03941 24000.00 11800.00 0.00 0.03964 24200.00 11800.00 0.00 0.03977 23600.00 12000.00 0.00 0.03998 23800.00 12000.00 0.00 0.04023 24000.00 12000.00 0.00 0.04042 24200.00 12000.00 0.00 0.04048 23600.00 12200.00 0.00 0.04077 23800.00 12200.00 0.00 0.04095 24000.00 12200.00 0.00 0.04106 24200.00 12200.00 0.00 0.04109 23600.00 12400.00 0.00 0.04141 23800.00 12400.00 0.00 0.04154 24000.00 12400.00 0.00 0.04164 24200.00 12400.00 0.00 0.04172 23600.00 12600.00 0.00 0.04195 23800.00 12600.00 0.00 0.04209 24000.00 12600.00 0.00 0.04224 24200.00 12600.00 0.00 0.04234 23600.00 12800.00 0.00 0.04264 23800.00 12800.00 0.00 0.04279 24000.00 12800.00 0.00 0.04293 24200.00 12800.00 0.00 0.04298 23400.00 13000.00 0.00 0.04306 23600.00 13000.00 0.00 0.04335 23800.00 13000.00 0.00 0.04347 24000.00 13000.00 0.00 0.04356 23400.00 13200.00 0.00 0.04374 23600.00 13200.00 0.00 0.04398 23800.00 13200.00 0.00 0.04406 24000.00 13200.00 0.00 0.04414 23400.00 13400.00 0.00 0.04440 23600.00 13400.00 0.00 0.04461 23800.00 13400.00 0.00 0.04471 24000.00 13400.00 0.00 0.04483 24200.00 13400.00 0.00 0.04485 23400.00 13600.00 0.00 0.04517 23600.00 13600.00 0.00 0.04541 23800.00 13600.00 0.00 0.04555 24000.00 13600.00 0.00 0.04571 24200.00 13600.00 0.00 0.04574 23400.00 13800.00 0.00 0.04597 23600.00 13800.00 0.00 0.04625 23800.00 13800.00 0.00 0.04643 24000.00 13800.00 0.00 0.04659 24200.00 13800.00 0.00 0.04660 23400.00 14000.00 0.00 0.04680 23600.00 14000.00 0.00 0.04712 23800.00 14000.00 0.00 0.04732 24000.00 14000.00 0.00 0.04750 23400.00 14200.00 0.00 0.04782 23600.00 14200.00 0.00 0.04818 23800.00 14200.00 0.00 0.04842 24000.00 14200.00 0.00 0.04859 23400.00 14400.00 0.00 0.04896 23600.00 14400.00 0.00 0.04934 23800.00 14400.00 0.00 0.04957 24000.00 14400.00 0.00 0.04969 23400.00 14600.00 0.00 0.05006 23600.00 14600.00 0.00 0.05043 23800.00 14600.00 0.00 0.05063 24000.00 14600.00 0.00 0.05070 23400.00 14800.00 0.00 0.05103 23600.00 14800.00 0.00 0.05143 23800.00 14800.00 0.00 0.05163 24000.00 14800.00 0.00 0.05169 23400.00 15000.00 0.00 0.05219 23600.00 15000.00 0.00 0.05264 23800.00 15000.00 0.00 0.05281 23400.00 15200.00 0.00 0.05351 23600.00 15200.00 0.00 0.05390 23800.00 15200.00 0.00 0.05395 23200.00 15400.00 0.00 0.05403 23400.00 15400.00 0.00 0.05484 23600.00 15400.00 0.00 0.05514 23200.00 15600.00 0.00 0.05534 23400.00 15600.00 0.00 0.05609 23600.00 15600.00 0.00 0.05631 23200.00 15800.00 0.00 0.05653 23400.00 15800.00 0.00 0.05724 23600.00 15800.00 0.00 0.05742 23200.00 16000.00 0.00 0.05783 23400.00 16000.00 0.00 0.05853 23600.00 16000.00 0.00 0.05870 23200.00 16200.00 0.00 0.05905 23400.00 16200.00 0.00 0.05974 23600.00 16200.00 0.00 0.05989 23200.00 16400.00 0.00 0.06036 23400.00 16400.00 0.00 0.06099 23600.00 16400.00 0.00 0.06109 23200.00 16600.00 0.00 0.06159 23400.00 16600.00 0.00 0.06215 23600.00 16600.00 0.00 0.06221 23200.00 16800.00 0.00 0.06270 23400.00 16800.00 0.00 0.06323 23600.00 16800.00 0.00 0.06328 23200.00 17000.00 0.00 0.06401 23400.00 17000.00 0.00 0.06455 23600.00 17000.00 0.00 0.06457 23200.00 17200.00 0.00 0.06532 23400.00 17200.00 0.00 0.06583 23200.00 17400.00 0.00 0.06661 23400.00 17400.00 0.00 0.06711 23200.00 17600.00 0.00 0.06801 23400.00 17600.00 0.00 0.06853 23200.00 17800.00 0.00 0.06954 23400.00 17800.00 0.00 0.07007 23200.00 18000.00 0.00 0.07111 23400.00 18000.00 0.00 0.07162 23200.00 18200.00 0.00 0.07269 23400.00 18200.00 0.00 0.07315 23200.00 18400.00 0.00 0.07421 23400.00 18400.00 0.00 0.07461 23200.00 18600.00 0.00 0.07568 23400.00 18600.00 0.00 0.07603 23200.00 18800.00 0.00 0.07715 23400.00 18800.00 0.00 0.07750 23200.00 19000.00 0.00 0.07875 23400.00 19000.00 0.00 0.07912 23000.00 19200.00 0.00 0.07922 23200.00 19200.00 0.00 0.08065 23400.00 19200.00 0.00 0.08098 23000.00 19400.00 0.00 0.08108 23200.00 19400.00 0.00 0.08244 23400.00 19400.00 0.00 0.08268 23000.00 19600.00 0.00 0.08286 23200.00 19600.00 0.00 0.08418 23400.00 19600.00 0.00 0.08436 23000.00 19800.00 0.00 0.08458 23200.00 19800.00 0.00 0.08589 23400.00 19800.00 0.00 0.08602 23000.00 20000.00 0.00 0.08629 23200.00 20000.00 0.00 0.08757 23400.00 20000.00 0.00 0.08761 23000.00 20200.00 0.00 0.08802 23200.00 20200.00 0.00 0.08923 23000.00 20400.00 0.00 0.08971 23200.00 20400.00 0.00 0.09089 23000.00 20600.00 0.00 0.09159 23200.00 20600.00 0.00 0.09273 23000.00 20800.00 0.00 0.09341 23200.00 20800.00 0.00 0.09450 23000.00 21000.00 0.00 0.09545 23200.00 21000.00 0.00 0.09648 23000.00 21200.00 0.00 0.09758 23200.00 21200.00 0.00 0.09854 23000.00 21400.00 0.00 0.09983 23200.00 21400.00 0.00 0.10069 23000.00 21600.00 0.00 0.10214 23200.00 21600.00 0.00 0.10290 23000.00 21800.00 0.00 0.10432 23200.00 21800.00 0.00 0.10498 23000.00 22000.00 0.00 0.10664 23200.00 22000.00 0.00 0.10714 23000.00 22200.00 0.00 0.10901 23200.00 22200.00 0.00 0.10926 23000.00 22400.00 0.00 0.11144 23000.00 22600.00 0.00 0.11336 23000.00 22800.00 0.00 0.11515 23000.00 23000.00 0.00 0.11598 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22915.12 23061.25 42.14 0.11633 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/582.ctf NX, NY, NZ: 512 512 1 MODE : real