CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/581.mrc Output diagnostic file name Micrographs_0601/581.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/581.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2484.000 7814.000 Mean, RMS : 4576.726 227.4438 TITLE 1: emi2mrc: 581.emi Mon Jan 06 19:12:14 2014 TITLE 2: kV 300, Mag 78000x, Def 23460, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X -35.091, Y 230.824, Z -40.486, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02435 11200.00 10000.00 0.00 -0.02417 11400.00 10000.00 0.00 -0.02392 11600.00 10000.00 0.00 -0.02381 11800.00 10000.00 0.00 -0.02381 12200.00 10000.00 0.00 -0.02380 12400.00 10000.00 0.00 -0.02363 12600.00 10000.00 0.00 -0.02349 12800.00 10000.00 0.00 -0.02321 13000.00 10000.00 0.00 -0.02287 13200.00 10000.00 0.00 -0.02261 13400.00 10000.00 0.00 -0.02225 13600.00 10000.00 0.00 -0.02169 13800.00 10000.00 0.00 -0.02118 14000.00 10000.00 0.00 -0.02077 14200.00 10000.00 0.00 -0.02021 14400.00 10000.00 0.00 -0.01953 14600.00 10000.00 0.00 -0.01884 14800.00 10000.00 0.00 -0.01818 15000.00 10000.00 0.00 -0.01762 15200.00 10000.00 0.00 -0.01693 15400.00 10000.00 0.00 -0.01617 15600.00 10000.00 0.00 -0.01556 15800.00 10000.00 0.00 -0.01499 16000.00 10000.00 0.00 -0.01431 16200.00 10000.00 0.00 -0.01339 16400.00 10000.00 0.00 -0.01241 16600.00 10000.00 0.00 -0.01147 16800.00 10000.00 0.00 -0.01053 17000.00 10000.00 0.00 -0.00967 17200.00 10000.00 0.00 -0.00878 17400.00 10000.00 0.00 -0.00781 17600.00 10000.00 0.00 -0.00692 17800.00 10000.00 0.00 -0.00601 18000.00 10000.00 0.00 -0.00515 18200.00 10000.00 0.00 -0.00438 18400.00 10000.00 0.00 -0.00331 18600.00 10000.00 0.00 -0.00203 18800.00 10000.00 0.00 -0.00087 19000.00 10000.00 0.00 0.00028 19200.00 10000.00 0.00 0.00143 19400.00 10000.00 0.00 0.00261 19600.00 10000.00 0.00 0.00376 19800.00 10000.00 0.00 0.00483 20000.00 10000.00 0.00 0.00579 20200.00 10000.00 0.00 0.00674 20400.00 10000.00 0.00 0.00786 20600.00 10000.00 0.00 0.00899 20800.00 10000.00 0.00 0.01011 21000.00 10000.00 0.00 0.01147 21200.00 10000.00 0.00 0.01300 21400.00 10000.00 0.00 0.01459 21600.00 10000.00 0.00 0.01629 21800.00 10000.00 0.00 0.01784 22000.00 10000.00 0.00 0.01945 22200.00 10000.00 0.00 0.02138 22400.00 10000.00 0.00 0.02332 22600.00 10000.00 0.00 0.02542 22800.00 10000.00 0.00 0.02762 23000.00 10000.00 0.00 0.02927 23200.00 10000.00 0.00 0.03029 23400.00 10000.00 0.00 0.03090 23600.00 10000.00 0.00 0.03119 23800.00 10000.00 0.00 0.03138 24000.00 10000.00 0.00 0.03151 23600.00 10200.00 0.00 0.03151 23800.00 10200.00 0.00 0.03171 24000.00 10200.00 0.00 0.03182 23800.00 10400.00 0.00 0.03199 24000.00 10400.00 0.00 0.03206 23600.00 10600.00 0.00 0.03213 23800.00 10600.00 0.00 0.03233 24000.00 10600.00 0.00 0.03233 23600.00 10800.00 0.00 0.03258 23800.00 10800.00 0.00 0.03273 23600.00 11000.00 0.00 0.03296 23800.00 11000.00 0.00 0.03306 23600.00 11200.00 0.00 0.03336 23800.00 11200.00 0.00 0.03344 23400.00 11400.00 0.00 0.03364 23600.00 11400.00 0.00 0.03396 23800.00 11400.00 0.00 0.03400 23400.00 11600.00 0.00 0.03430 23600.00 11600.00 0.00 0.03461 23800.00 11600.00 0.00 0.03462 23400.00 11800.00 0.00 0.03507 23600.00 11800.00 0.00 0.03536 23400.00 12000.00 0.00 0.03583 23600.00 12000.00 0.00 0.03609 23200.00 12200.00 0.00 0.03611 23400.00 12200.00 0.00 0.03659 23600.00 12200.00 0.00 0.03680 23200.00 12400.00 0.00 0.03682 23400.00 12400.00 0.00 0.03727 23600.00 12400.00 0.00 0.03744 23200.00 12600.00 0.00 0.03750 23400.00 12600.00 0.00 0.03794 23600.00 12600.00 0.00 0.03810 23200.00 12800.00 0.00 0.03830 23400.00 12800.00 0.00 0.03875 23600.00 12800.00 0.00 0.03889 23200.00 13000.00 0.00 0.03909 23400.00 13000.00 0.00 0.03951 23600.00 13000.00 0.00 0.03962 23200.00 13200.00 0.00 0.04011 23400.00 13200.00 0.00 0.04047 23600.00 13200.00 0.00 0.04055 23200.00 13400.00 0.00 0.04116 23400.00 13400.00 0.00 0.04147 23600.00 13400.00 0.00 0.04156 23200.00 13600.00 0.00 0.04201 23400.00 13600.00 0.00 0.04231 23600.00 13600.00 0.00 0.04241 23200.00 13800.00 0.00 0.04279 23400.00 13800.00 0.00 0.04309 23600.00 13800.00 0.00 0.04318 23200.00 14000.00 0.00 0.04354 23400.00 14000.00 0.00 0.04387 23600.00 14000.00 0.00 0.04399 23200.00 14200.00 0.00 0.04442 23400.00 14200.00 0.00 0.04482 23600.00 14200.00 0.00 0.04496 23200.00 14400.00 0.00 0.04543 23400.00 14400.00 0.00 0.04586 23600.00 14400.00 0.00 0.04596 23200.00 14600.00 0.00 0.04659 23400.00 14600.00 0.00 0.04695 23200.00 14800.00 0.00 0.04766 23400.00 14800.00 0.00 0.04793 23000.00 15000.00 0.00 0.04798 23200.00 15000.00 0.00 0.04875 23400.00 15000.00 0.00 0.04898 23000.00 15200.00 0.00 0.04922 23200.00 15200.00 0.00 0.04993 23400.00 15200.00 0.00 0.05014 23000.00 15400.00 0.00 0.05037 23200.00 15400.00 0.00 0.05103 23400.00 15400.00 0.00 0.05123 23600.00 15400.00 0.00 0.05124 23000.00 15600.00 0.00 0.05151 23200.00 15600.00 0.00 0.05221 23400.00 15600.00 0.00 0.05246 23600.00 15600.00 0.00 0.05250 23000.00 15800.00 0.00 0.05268 23200.00 15800.00 0.00 0.05343 23400.00 15800.00 0.00 0.05369 23600.00 15800.00 0.00 0.05373 23000.00 16000.00 0.00 0.05380 23200.00 16000.00 0.00 0.05455 23400.00 16000.00 0.00 0.05478 23600.00 16000.00 0.00 0.05480 23000.00 16200.00 0.00 0.05498 23200.00 16200.00 0.00 0.05570 23400.00 16200.00 0.00 0.05591 23600.00 16200.00 0.00 0.05594 23000.00 16400.00 0.00 0.05635 23200.00 16400.00 0.00 0.05704 23400.00 16400.00 0.00 0.05724 23600.00 16400.00 0.00 0.05726 23000.00 16600.00 0.00 0.05769 23200.00 16600.00 0.00 0.05834 23400.00 16600.00 0.00 0.05855 23000.00 16800.00 0.00 0.05901 23200.00 16800.00 0.00 0.05966 23400.00 16800.00 0.00 0.05987 23000.00 17000.00 0.00 0.06022 23200.00 17000.00 0.00 0.06086 23400.00 17000.00 0.00 0.06104 23000.00 17200.00 0.00 0.06151 23200.00 17200.00 0.00 0.06214 23400.00 17200.00 0.00 0.06228 23000.00 17400.00 0.00 0.06295 23200.00 17400.00 0.00 0.06358 23400.00 17400.00 0.00 0.06368 23000.00 17600.00 0.00 0.06427 23200.00 17600.00 0.00 0.06491 23400.00 17600.00 0.00 0.06497 23000.00 17800.00 0.00 0.06577 23200.00 17800.00 0.00 0.06641 23400.00 17800.00 0.00 0.06645 23000.00 18000.00 0.00 0.06721 23200.00 18000.00 0.00 0.06785 23000.00 18200.00 0.00 0.06877 23200.00 18200.00 0.00 0.06939 23000.00 18400.00 0.00 0.07034 23200.00 18400.00 0.00 0.07092 23000.00 18600.00 0.00 0.07182 23200.00 18600.00 0.00 0.07234 23000.00 18800.00 0.00 0.07351 23200.00 18800.00 0.00 0.07396 23000.00 19000.00 0.00 0.07512 23200.00 19000.00 0.00 0.07549 23000.00 19200.00 0.00 0.07688 23200.00 19200.00 0.00 0.07720 23000.00 19400.00 0.00 0.07859 23200.00 19400.00 0.00 0.07886 23000.00 19600.00 0.00 0.08030 23200.00 19600.00 0.00 0.08055 23000.00 19800.00 0.00 0.08219 23200.00 19800.00 0.00 0.08237 22800.00 20000.00 0.00 0.08237 23000.00 20000.00 0.00 0.08404 23200.00 20000.00 0.00 0.08410 22800.00 20200.00 0.00 0.08419 23000.00 20200.00 0.00 0.08579 22800.00 20400.00 0.00 0.08587 23000.00 20400.00 0.00 0.08737 22800.00 20600.00 0.00 0.08772 23000.00 20600.00 0.00 0.08916 22800.00 20800.00 0.00 0.08953 23000.00 20800.00 0.00 0.09092 22800.00 21000.00 0.00 0.09148 23000.00 21000.00 0.00 0.09281 22800.00 21200.00 0.00 0.09351 23000.00 21200.00 0.00 0.09472 22800.00 21400.00 0.00 0.09538 23000.00 21400.00 0.00 0.09646 22800.00 21600.00 0.00 0.09719 23000.00 21600.00 0.00 0.09817 22800.00 21800.00 0.00 0.09899 23000.00 21800.00 0.00 0.09986 22800.00 22000.00 0.00 0.10084 23000.00 22000.00 0.00 0.10157 22800.00 22200.00 0.00 0.10251 23000.00 22200.00 0.00 0.10309 22800.00 22400.00 0.00 0.10427 23000.00 22400.00 0.00 0.10459 22800.00 22600.00 0.00 0.10575 22800.00 22800.00 0.00 0.10703 22800.00 23000.00 0.00 0.10737 22800.00 23000.00 5.00 0.10749 22800.00 23000.00 10.00 0.10759 22800.00 23000.00 15.00 0.10769 22800.00 23000.00 20.00 0.10776 22800.00 23000.00 25.00 0.10781 22800.00 23000.00 30.00 0.10783 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22788.60 22988.87 32.64 0.10784 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/581.ctf NX, NY, NZ: 512 512 1 MODE : real