CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/580.mrc Output diagnostic file name Micrographs_0601/580.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/580.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2386.000 8040.000 Mean, RMS : 4582.452 223.0018 TITLE 1: emi2mrc: 580.emi Mon Jan 06 19:11:37 2014 TITLE 2: kV 300, Mag 78000x, Def 23460, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X -33.473, Y 228.514, Z -40.486, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01583 12800.00 10000.00 0.00 -0.01569 13000.00 10000.00 0.00 -0.01554 13200.00 10000.00 0.00 -0.01547 13400.00 10000.00 0.00 -0.01538 13600.00 10000.00 0.00 -0.01512 13800.00 10000.00 0.00 -0.01484 14000.00 10000.00 0.00 -0.01449 14200.00 10000.00 0.00 -0.01405 14400.00 10000.00 0.00 -0.01371 14600.00 10000.00 0.00 -0.01331 14800.00 10000.00 0.00 -0.01290 15000.00 10000.00 0.00 -0.01259 15200.00 10000.00 0.00 -0.01203 15400.00 10000.00 0.00 -0.01125 15600.00 10000.00 0.00 -0.01055 15800.00 10000.00 0.00 -0.01001 16000.00 10000.00 0.00 -0.00941 16200.00 10000.00 0.00 -0.00860 16400.00 10000.00 0.00 -0.00789 16600.00 10000.00 0.00 -0.00715 16800.00 10000.00 0.00 -0.00632 17000.00 10000.00 0.00 -0.00547 17200.00 10000.00 0.00 -0.00461 17400.00 10000.00 0.00 -0.00380 17600.00 10000.00 0.00 -0.00282 17800.00 10000.00 0.00 -0.00183 18000.00 10000.00 0.00 -0.00077 18200.00 10000.00 0.00 0.00050 18400.00 10000.00 0.00 0.00165 18600.00 10000.00 0.00 0.00272 18800.00 10000.00 0.00 0.00383 19000.00 10000.00 0.00 0.00490 19200.00 10000.00 0.00 0.00603 19400.00 10000.00 0.00 0.00721 19600.00 10000.00 0.00 0.00827 19800.00 10000.00 0.00 0.00925 20000.00 10000.00 0.00 0.01030 20200.00 10000.00 0.00 0.01136 20400.00 10000.00 0.00 0.01251 20600.00 10000.00 0.00 0.01384 20800.00 10000.00 0.00 0.01521 21000.00 10000.00 0.00 0.01663 21200.00 10000.00 0.00 0.01815 21400.00 10000.00 0.00 0.01977 21600.00 10000.00 0.00 0.02144 21800.00 10000.00 0.00 0.02308 22000.00 10000.00 0.00 0.02493 22200.00 10000.00 0.00 0.02697 22400.00 10000.00 0.00 0.02894 22600.00 10000.00 0.00 0.03093 22800.00 10000.00 0.00 0.03314 23000.00 10000.00 0.00 0.03514 23200.00 10000.00 0.00 0.03625 23400.00 10000.00 0.00 0.03662 23600.00 10000.00 0.00 0.03670 23400.00 10200.00 0.00 0.03697 23600.00 10200.00 0.00 0.03706 23400.00 10400.00 0.00 0.03731 23600.00 10400.00 0.00 0.03744 23400.00 10600.00 0.00 0.03777 23600.00 10600.00 0.00 0.03790 23400.00 10800.00 0.00 0.03825 23600.00 10800.00 0.00 0.03836 23400.00 11000.00 0.00 0.03871 23600.00 11000.00 0.00 0.03883 23400.00 11200.00 0.00 0.03920 23600.00 11200.00 0.00 0.03935 23200.00 11400.00 0.00 0.03943 23400.00 11400.00 0.00 0.03987 23600.00 11400.00 0.00 0.04003 23200.00 11600.00 0.00 0.04003 23400.00 11600.00 0.00 0.04051 23600.00 11600.00 0.00 0.04065 23400.00 11800.00 0.00 0.04106 23600.00 11800.00 0.00 0.04117 23400.00 12000.00 0.00 0.04150 23600.00 12000.00 0.00 0.04157 23400.00 12200.00 0.00 0.04194 23600.00 12200.00 0.00 0.04200 23200.00 12400.00 0.00 0.04210 23400.00 12400.00 0.00 0.04259 23600.00 12400.00 0.00 0.04267 23200.00 12600.00 0.00 0.04289 23400.00 12600.00 0.00 0.04342 23600.00 12600.00 0.00 0.04349 23200.00 12800.00 0.00 0.04377 23400.00 12800.00 0.00 0.04430 23600.00 12800.00 0.00 0.04433 23200.00 13000.00 0.00 0.04458 23400.00 13000.00 0.00 0.04507 23200.00 13200.00 0.00 0.04542 23400.00 13200.00 0.00 0.04585 23200.00 13400.00 0.00 0.04619 23400.00 13400.00 0.00 0.04658 23200.00 13600.00 0.00 0.04703 23400.00 13600.00 0.00 0.04742 23200.00 13800.00 0.00 0.04788 23400.00 13800.00 0.00 0.04822 23200.00 14000.00 0.00 0.04866 23400.00 14000.00 0.00 0.04897 23200.00 14200.00 0.00 0.04970 23400.00 14200.00 0.00 0.05001 23200.00 14400.00 0.00 0.05073 23400.00 14400.00 0.00 0.05099 23200.00 14600.00 0.00 0.05160 23400.00 14600.00 0.00 0.05178 23200.00 14800.00 0.00 0.05238 23400.00 14800.00 0.00 0.05253 23200.00 15000.00 0.00 0.05319 23400.00 15000.00 0.00 0.05333 23200.00 15200.00 0.00 0.05416 23400.00 15200.00 0.00 0.05428 23200.00 15400.00 0.00 0.05519 23400.00 15400.00 0.00 0.05526 23200.00 15600.00 0.00 0.05615 23400.00 15600.00 0.00 0.05619 23200.00 15800.00 0.00 0.05707 23400.00 15800.00 0.00 0.05714 23000.00 16000.00 0.00 0.05716 23200.00 16000.00 0.00 0.05808 23400.00 16000.00 0.00 0.05819 23000.00 16200.00 0.00 0.05824 23200.00 16200.00 0.00 0.05919 23400.00 16200.00 0.00 0.05930 23000.00 16400.00 0.00 0.05939 23200.00 16400.00 0.00 0.06035 23400.00 16400.00 0.00 0.06042 23000.00 16600.00 0.00 0.06048 23200.00 16600.00 0.00 0.06145 23400.00 16600.00 0.00 0.06151 23000.00 16800.00 0.00 0.06166 23200.00 16800.00 0.00 0.06264 23400.00 16800.00 0.00 0.06271 23000.00 17000.00 0.00 0.06285 23200.00 17000.00 0.00 0.06381 23400.00 17000.00 0.00 0.06386 23000.00 17200.00 0.00 0.06406 23200.00 17200.00 0.00 0.06503 23400.00 17200.00 0.00 0.06509 23000.00 17400.00 0.00 0.06558 23200.00 17400.00 0.00 0.06658 23400.00 17400.00 0.00 0.06666 23000.00 17600.00 0.00 0.06712 23200.00 17600.00 0.00 0.06816 23400.00 17600.00 0.00 0.06821 23000.00 17800.00 0.00 0.06868 23200.00 17800.00 0.00 0.06971 23400.00 17800.00 0.00 0.06971 23000.00 18000.00 0.00 0.07020 23200.00 18000.00 0.00 0.07119 23000.00 18200.00 0.00 0.07181 23200.00 18200.00 0.00 0.07277 23000.00 18400.00 0.00 0.07349 23200.00 18400.00 0.00 0.07445 23000.00 18600.00 0.00 0.07519 23200.00 18600.00 0.00 0.07616 23000.00 18800.00 0.00 0.07684 23200.00 18800.00 0.00 0.07781 23000.00 19000.00 0.00 0.07849 23200.00 19000.00 0.00 0.07943 23000.00 19200.00 0.00 0.08025 23200.00 19200.00 0.00 0.08116 23000.00 19400.00 0.00 0.08189 23200.00 19400.00 0.00 0.08280 23000.00 19600.00 0.00 0.08387 23200.00 19600.00 0.00 0.08479 23000.00 19800.00 0.00 0.08597 23200.00 19800.00 0.00 0.08683 23000.00 20000.00 0.00 0.08790 23200.00 20000.00 0.00 0.08868 23000.00 20200.00 0.00 0.08987 23200.00 20200.00 0.00 0.09060 23000.00 20400.00 0.00 0.09194 23200.00 20400.00 0.00 0.09265 23000.00 20600.00 0.00 0.09432 23200.00 20600.00 0.00 0.09497 23000.00 20800.00 0.00 0.09669 23200.00 20800.00 0.00 0.09723 23000.00 21000.00 0.00 0.09918 23200.00 21000.00 0.00 0.09960 23000.00 21200.00 0.00 0.10167 23200.00 21200.00 0.00 0.10199 23000.00 21400.00 0.00 0.10433 23200.00 21400.00 0.00 0.10451 22800.00 21600.00 0.00 0.10454 23000.00 21600.00 0.00 0.10711 23200.00 21600.00 0.00 0.10714 22800.00 21800.00 0.00 0.10740 23000.00 21800.00 0.00 0.11000 22800.00 22000.00 0.00 0.11061 23000.00 22000.00 0.00 0.11314 22800.00 22200.00 0.00 0.11378 23000.00 22200.00 0.00 0.11614 22800.00 22400.00 0.00 0.11717 23000.00 22400.00 0.00 0.11928 22800.00 22600.00 0.00 0.12023 23000.00 22600.00 0.00 0.12196 22800.00 22800.00 0.00 0.12333 23000.00 22800.00 0.00 0.12434 22800.00 23000.00 0.00 0.12512 22800.00 23000.00 5.00 0.12527 22800.00 23000.00 10.00 0.12541 22800.00 23000.00 15.00 0.12552 22800.00 23000.00 20.00 0.12561 22800.00 23000.00 25.00 0.12567 22800.00 23000.00 30.00 0.12570 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22863.87 23008.09 30.00 0.12593 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/580.ctf NX, NY, NZ: 512 512 1 MODE : real