CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/577.mrc Output diagnostic file name Micrographs_0601/577.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/577.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2374.000 8026.000 Mean, RMS : 4526.395 221.9515 TITLE 1: emi2mrc: 577.emi Mon Jan 06 19:09:04 2014 TITLE 2: kV 300, Mag 78000x, Def 22810, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X -27.364, Y 220.406, Z -42.772, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02243 13600.00 10000.00 0.00 -0.02212 13800.00 10000.00 0.00 -0.02198 14000.00 10000.00 0.00 -0.02185 14200.00 10000.00 0.00 -0.02160 14400.00 10000.00 0.00 -0.02130 14600.00 10000.00 0.00 -0.02086 14800.00 10000.00 0.00 -0.02030 15000.00 10000.00 0.00 -0.01977 15200.00 10000.00 0.00 -0.01922 15400.00 10000.00 0.00 -0.01859 15600.00 10000.00 0.00 -0.01798 15800.00 10000.00 0.00 -0.01730 16000.00 10000.00 0.00 -0.01662 16200.00 10000.00 0.00 -0.01587 16400.00 10000.00 0.00 -0.01504 16600.00 10000.00 0.00 -0.01416 16800.00 10000.00 0.00 -0.01318 17000.00 10000.00 0.00 -0.01230 17200.00 10000.00 0.00 -0.01141 17400.00 10000.00 0.00 -0.01055 17600.00 10000.00 0.00 -0.00975 17800.00 10000.00 0.00 -0.00901 18000.00 10000.00 0.00 -0.00825 18200.00 10000.00 0.00 -0.00738 18400.00 10000.00 0.00 -0.00661 18600.00 10000.00 0.00 -0.00585 18800.00 10000.00 0.00 -0.00511 19000.00 10000.00 0.00 -0.00432 19200.00 10000.00 0.00 -0.00324 19400.00 10000.00 0.00 -0.00207 19600.00 10000.00 0.00 -0.00094 19800.00 10000.00 0.00 0.00012 20000.00 10000.00 0.00 0.00110 20200.00 10000.00 0.00 0.00212 20400.00 10000.00 0.00 0.00319 20600.00 10000.00 0.00 0.00423 20800.00 10000.00 0.00 0.00521 21000.00 10000.00 0.00 0.00631 21200.00 10000.00 0.00 0.00750 21400.00 10000.00 0.00 0.00867 21600.00 10000.00 0.00 0.00995 21800.00 10000.00 0.00 0.01135 22000.00 10000.00 0.00 0.01268 22200.00 10000.00 0.00 0.01407 22400.00 10000.00 0.00 0.01562 22600.00 10000.00 0.00 0.01725 22800.00 10000.00 0.00 0.01888 23000.00 10000.00 0.00 0.02047 23200.00 10000.00 0.00 0.02212 23400.00 10000.00 0.00 0.02382 23600.00 10000.00 0.00 0.02542 23800.00 10000.00 0.00 0.02698 24000.00 10000.00 0.00 0.02828 24200.00 10000.00 0.00 0.02913 24400.00 10000.00 0.00 0.02968 24600.00 10000.00 0.00 0.03009 24800.00 10000.00 0.00 0.03020 24600.00 10200.00 0.00 0.03056 24800.00 10200.00 0.00 0.03061 24600.00 10400.00 0.00 0.03091 24800.00 10400.00 0.00 0.03092 24400.00 10600.00 0.00 0.03093 24600.00 10600.00 0.00 0.03123 24800.00 10600.00 0.00 0.03123 24400.00 10800.00 0.00 0.03126 24600.00 10800.00 0.00 0.03154 24800.00 10800.00 0.00 0.03155 24600.00 11000.00 0.00 0.03183 24800.00 11000.00 0.00 0.03184 24600.00 11200.00 0.00 0.03212 24800.00 11200.00 0.00 0.03215 24400.00 11400.00 0.00 0.03222 24600.00 11400.00 0.00 0.03249 24800.00 11400.00 0.00 0.03250 24400.00 11600.00 0.00 0.03258 24600.00 11600.00 0.00 0.03282 24400.00 11800.00 0.00 0.03298 24600.00 11800.00 0.00 0.03322 24800.00 11800.00 0.00 0.03323 24400.00 12000.00 0.00 0.03343 24600.00 12000.00 0.00 0.03368 24800.00 12000.00 0.00 0.03371 24400.00 12200.00 0.00 0.03391 24600.00 12200.00 0.00 0.03418 24800.00 12200.00 0.00 0.03424 24400.00 12400.00 0.00 0.03442 24600.00 12400.00 0.00 0.03472 24800.00 12400.00 0.00 0.03481 24400.00 12600.00 0.00 0.03498 24600.00 12600.00 0.00 0.03531 24800.00 12600.00 0.00 0.03542 24400.00 12800.00 0.00 0.03567 24600.00 12800.00 0.00 0.03603 24800.00 12800.00 0.00 0.03614 24400.00 13000.00 0.00 0.03645 24600.00 13000.00 0.00 0.03680 24800.00 13000.00 0.00 0.03688 24400.00 13200.00 0.00 0.03723 24600.00 13200.00 0.00 0.03756 24800.00 13200.00 0.00 0.03760 24400.00 13400.00 0.00 0.03794 24600.00 13400.00 0.00 0.03826 24800.00 13400.00 0.00 0.03829 24400.00 13600.00 0.00 0.03869 24600.00 13600.00 0.00 0.03899 24800.00 13600.00 0.00 0.03900 24400.00 13800.00 0.00 0.03935 24600.00 13800.00 0.00 0.03964 24800.00 13800.00 0.00 0.03966 24400.00 14000.00 0.00 0.04006 24600.00 14000.00 0.00 0.04037 24800.00 14000.00 0.00 0.04043 24400.00 14200.00 0.00 0.04090 24600.00 14200.00 0.00 0.04123 24800.00 14200.00 0.00 0.04131 24400.00 14400.00 0.00 0.04166 24600.00 14400.00 0.00 0.04202 24800.00 14400.00 0.00 0.04206 24400.00 14600.00 0.00 0.04255 24600.00 14600.00 0.00 0.04287 24200.00 14800.00 0.00 0.04290 24400.00 14800.00 0.00 0.04350 24600.00 14800.00 0.00 0.04377 24200.00 15000.00 0.00 0.04392 24400.00 15000.00 0.00 0.04452 24600.00 15000.00 0.00 0.04476 24200.00 15200.00 0.00 0.04494 24400.00 15200.00 0.00 0.04553 24600.00 15200.00 0.00 0.04574 24200.00 15400.00 0.00 0.04598 24400.00 15400.00 0.00 0.04651 24600.00 15400.00 0.00 0.04667 24200.00 15600.00 0.00 0.04696 24400.00 15600.00 0.00 0.04744 24600.00 15600.00 0.00 0.04761 24200.00 15800.00 0.00 0.04781 24400.00 15800.00 0.00 0.04831 24600.00 15800.00 0.00 0.04849 24200.00 16000.00 0.00 0.04881 24400.00 16000.00 0.00 0.04933 24600.00 16000.00 0.00 0.04952 24200.00 16200.00 0.00 0.04994 24400.00 16200.00 0.00 0.05046 24600.00 16200.00 0.00 0.05062 24200.00 16400.00 0.00 0.05125 24400.00 16400.00 0.00 0.05174 24600.00 16400.00 0.00 0.05189 24200.00 16600.00 0.00 0.05258 24400.00 16600.00 0.00 0.05302 24600.00 16600.00 0.00 0.05316 24200.00 16800.00 0.00 0.05383 24400.00 16800.00 0.00 0.05425 24600.00 16800.00 0.00 0.05441 24200.00 17000.00 0.00 0.05509 24400.00 17000.00 0.00 0.05552 24600.00 17000.00 0.00 0.05569 24200.00 17200.00 0.00 0.05635 24400.00 17200.00 0.00 0.05678 24600.00 17200.00 0.00 0.05692 24200.00 17400.00 0.00 0.05769 24400.00 17400.00 0.00 0.05808 24600.00 17400.00 0.00 0.05819 24200.00 17600.00 0.00 0.05898 24400.00 17600.00 0.00 0.05934 24600.00 17600.00 0.00 0.05945 24000.00 17800.00 0.00 0.05948 24200.00 17800.00 0.00 0.06032 24400.00 17800.00 0.00 0.06070 24600.00 17800.00 0.00 0.06084 24200.00 18000.00 0.00 0.06161 24400.00 18000.00 0.00 0.06202 24600.00 18000.00 0.00 0.06217 24200.00 18200.00 0.00 0.06293 24400.00 18200.00 0.00 0.06335 24600.00 18200.00 0.00 0.06347 24000.00 18400.00 0.00 0.06352 24200.00 18400.00 0.00 0.06437 24400.00 18400.00 0.00 0.06478 24600.00 18400.00 0.00 0.06484 24000.00 18600.00 0.00 0.06494 24200.00 18600.00 0.00 0.06578 24400.00 18600.00 0.00 0.06616 24600.00 18600.00 0.00 0.06619 24000.00 18800.00 0.00 0.06635 24200.00 18800.00 0.00 0.06718 24400.00 18800.00 0.00 0.06755 24600.00 18800.00 0.00 0.06756 24000.00 19000.00 0.00 0.06773 24200.00 19000.00 0.00 0.06856 24400.00 19000.00 0.00 0.06892 24600.00 19000.00 0.00 0.06892 24000.00 19200.00 0.00 0.06916 24200.00 19200.00 0.00 0.06996 24400.00 19200.00 0.00 0.07028 24000.00 19400.00 0.00 0.07053 24200.00 19400.00 0.00 0.07127 24400.00 19400.00 0.00 0.07154 24000.00 19600.00 0.00 0.07187 24200.00 19600.00 0.00 0.07258 24400.00 19600.00 0.00 0.07284 24000.00 19800.00 0.00 0.07331 24200.00 19800.00 0.00 0.07402 24400.00 19800.00 0.00 0.07428 24000.00 20000.00 0.00 0.07493 24200.00 20000.00 0.00 0.07560 24400.00 20000.00 0.00 0.07585 24000.00 20200.00 0.00 0.07641 24200.00 20200.00 0.00 0.07706 24400.00 20200.00 0.00 0.07728 24000.00 20400.00 0.00 0.07788 24200.00 20400.00 0.00 0.07850 24400.00 20400.00 0.00 0.07864 24000.00 20600.00 0.00 0.07944 24200.00 20600.00 0.00 0.07999 24400.00 20600.00 0.00 0.08004 24000.00 20800.00 0.00 0.08083 24200.00 20800.00 0.00 0.08133 24400.00 20800.00 0.00 0.08135 24000.00 21000.00 0.00 0.08240 24200.00 21000.00 0.00 0.08288 24000.00 21200.00 0.00 0.08388 24200.00 21200.00 0.00 0.08433 24000.00 21400.00 0.00 0.08547 24200.00 21400.00 0.00 0.08590 24000.00 21600.00 0.00 0.08720 24200.00 21600.00 0.00 0.08758 24000.00 21800.00 0.00 0.08883 24200.00 21800.00 0.00 0.08911 23800.00 22000.00 0.00 0.08925 24000.00 22000.00 0.00 0.09052 24200.00 22000.00 0.00 0.09069 23800.00 22200.00 0.00 0.09083 24000.00 22200.00 0.00 0.09200 24200.00 22200.00 0.00 0.09211 23800.00 22400.00 0.00 0.09239 24000.00 22400.00 0.00 0.09354 24200.00 22400.00 0.00 0.09359 23800.00 22600.00 0.00 0.09392 24000.00 22600.00 0.00 0.09506 23800.00 22800.00 0.00 0.09558 24000.00 22800.00 0.00 0.09669 23800.00 23000.00 0.00 0.09722 24000.00 23000.00 0.00 0.09828 23800.00 23200.00 0.00 0.09896 24000.00 23200.00 0.00 0.09992 23800.00 23400.00 0.00 0.10075 24000.00 23400.00 0.00 0.10148 23800.00 23600.00 0.00 0.10208 24000.00 23600.00 0.00 0.10250 23800.00 23800.00 0.00 0.10329 23800.00 24000.00 0.00 0.10341 23800.00 24000.00 5.00 0.10346 23800.00 24000.00 10.00 0.10350 23800.00 24000.00 15.00 0.10353 23800.00 24000.00 20.00 0.10355 23800.00 24000.00 25.00 0.10356 23800.00 24000.00 30.00 0.10357 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23830.76 23911.56 37.27 0.10377 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/577.ctf NX, NY, NZ: 512 512 1 MODE : real