CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/576.mrc Output diagnostic file name Micrographs_0601/576.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/576.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2316.000 8542.000 Mean, RMS : 4525.988 221.9441 TITLE 1: emi2mrc: 576.emi Mon Jan 06 19:08:26 2014 TITLE 2: kV 300, Mag 78000x, Def 22810, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X -25.696, Y 218.011, Z -42.772, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01170 10600.00 10000.00 0.00 -0.01161 11000.00 10000.00 0.00 -0.01159 11400.00 10000.00 0.00 -0.01157 11600.00 10000.00 0.00 -0.01132 11800.00 10000.00 0.00 -0.01098 12000.00 10000.00 0.00 -0.01064 12200.00 10000.00 0.00 -0.01025 12400.00 10000.00 0.00 -0.01000 12600.00 10000.00 0.00 -0.00985 12800.00 10000.00 0.00 -0.00961 13000.00 10000.00 0.00 -0.00909 13200.00 10000.00 0.00 -0.00841 13400.00 10000.00 0.00 -0.00782 13600.00 10000.00 0.00 -0.00732 13800.00 10000.00 0.00 -0.00682 14000.00 10000.00 0.00 -0.00629 14200.00 10000.00 0.00 -0.00591 14400.00 10000.00 0.00 -0.00553 14600.00 10000.00 0.00 -0.00497 14800.00 10000.00 0.00 -0.00446 15000.00 10000.00 0.00 -0.00388 15200.00 10000.00 0.00 -0.00315 15400.00 10000.00 0.00 -0.00237 15600.00 10000.00 0.00 -0.00155 15800.00 10000.00 0.00 -0.00073 16000.00 10000.00 0.00 0.00008 16200.00 10000.00 0.00 0.00083 16400.00 10000.00 0.00 0.00172 16600.00 10000.00 0.00 0.00280 16800.00 10000.00 0.00 0.00392 17000.00 10000.00 0.00 0.00498 17200.00 10000.00 0.00 0.00600 17400.00 10000.00 0.00 0.00705 17600.00 10000.00 0.00 0.00815 17800.00 10000.00 0.00 0.00937 18000.00 10000.00 0.00 0.01072 18200.00 10000.00 0.00 0.01205 18400.00 10000.00 0.00 0.01335 18600.00 10000.00 0.00 0.01462 18800.00 10000.00 0.00 0.01585 19000.00 10000.00 0.00 0.01718 19200.00 10000.00 0.00 0.01858 19400.00 10000.00 0.00 0.01990 19600.00 10000.00 0.00 0.02124 19800.00 10000.00 0.00 0.02266 20000.00 10000.00 0.00 0.02417 20200.00 10000.00 0.00 0.02585 20400.00 10000.00 0.00 0.02784 20600.00 10000.00 0.00 0.03005 20800.00 10000.00 0.00 0.03215 21000.00 10000.00 0.00 0.03422 21200.00 10000.00 0.00 0.03642 21400.00 10000.00 0.00 0.03856 21600.00 10000.00 0.00 0.04021 21800.00 10000.00 0.00 0.04115 22000.00 10000.00 0.00 0.04170 22200.00 10000.00 0.00 0.04193 22000.00 10200.00 0.00 0.04218 22200.00 10200.00 0.00 0.04239 22000.00 10400.00 0.00 0.04286 22200.00 10400.00 0.00 0.04305 21800.00 10600.00 0.00 0.04307 22000.00 10600.00 0.00 0.04361 22200.00 10600.00 0.00 0.04379 22000.00 10800.00 0.00 0.04424 22200.00 10800.00 0.00 0.04441 22000.00 11000.00 0.00 0.04489 22200.00 11000.00 0.00 0.04506 21800.00 11200.00 0.00 0.04525 22000.00 11200.00 0.00 0.04575 22200.00 11200.00 0.00 0.04594 21800.00 11400.00 0.00 0.04612 22000.00 11400.00 0.00 0.04662 22200.00 11400.00 0.00 0.04679 21800.00 11600.00 0.00 0.04685 22000.00 11600.00 0.00 0.04732 22200.00 11600.00 0.00 0.04747 21800.00 11800.00 0.00 0.04771 22000.00 11800.00 0.00 0.04813 22200.00 11800.00 0.00 0.04824 21800.00 12000.00 0.00 0.04847 22000.00 12000.00 0.00 0.04885 22200.00 12000.00 0.00 0.04895 21800.00 12200.00 0.00 0.04917 22000.00 12200.00 0.00 0.04958 22200.00 12200.00 0.00 0.04974 21800.00 12400.00 0.00 0.05010 22000.00 12400.00 0.00 0.05054 22200.00 12400.00 0.00 0.05070 21800.00 12600.00 0.00 0.05093 22000.00 12600.00 0.00 0.05135 22200.00 12600.00 0.00 0.05149 21800.00 12800.00 0.00 0.05177 22000.00 12800.00 0.00 0.05221 22200.00 12800.00 0.00 0.05237 21800.00 13000.00 0.00 0.05278 22000.00 13000.00 0.00 0.05325 22200.00 13000.00 0.00 0.05342 21800.00 13200.00 0.00 0.05385 22000.00 13200.00 0.00 0.05431 22200.00 13200.00 0.00 0.05445 21800.00 13400.00 0.00 0.05477 22000.00 13400.00 0.00 0.05522 22200.00 13400.00 0.00 0.05536 21800.00 13600.00 0.00 0.05565 22000.00 13600.00 0.00 0.05613 22200.00 13600.00 0.00 0.05630 21800.00 13800.00 0.00 0.05667 22000.00 13800.00 0.00 0.05720 22200.00 13800.00 0.00 0.05736 21800.00 14000.00 0.00 0.05780 22000.00 14000.00 0.00 0.05834 22200.00 14000.00 0.00 0.05842 21800.00 14200.00 0.00 0.05895 22000.00 14200.00 0.00 0.05943 22200.00 14200.00 0.00 0.05944 21800.00 14400.00 0.00 0.06001 22000.00 14400.00 0.00 0.06045 21800.00 14600.00 0.00 0.06122 22000.00 14600.00 0.00 0.06162 21800.00 14800.00 0.00 0.06241 22000.00 14800.00 0.00 0.06279 21800.00 15000.00 0.00 0.06361 22000.00 15000.00 0.00 0.06396 21800.00 15200.00 0.00 0.06492 22000.00 15200.00 0.00 0.06523 21600.00 15400.00 0.00 0.06529 21800.00 15400.00 0.00 0.06623 22000.00 15400.00 0.00 0.06650 21600.00 15600.00 0.00 0.06671 21800.00 15600.00 0.00 0.06763 22000.00 15600.00 0.00 0.06786 21600.00 15800.00 0.00 0.06821 21800.00 15800.00 0.00 0.06910 22000.00 15800.00 0.00 0.06931 21600.00 16000.00 0.00 0.06981 21800.00 16000.00 0.00 0.07071 22000.00 16000.00 0.00 0.07091 21600.00 16200.00 0.00 0.07149 21800.00 16200.00 0.00 0.07239 22000.00 16200.00 0.00 0.07256 21600.00 16400.00 0.00 0.07326 21800.00 16400.00 0.00 0.07412 22000.00 16400.00 0.00 0.07426 21600.00 16600.00 0.00 0.07497 21800.00 16600.00 0.00 0.07582 22000.00 16600.00 0.00 0.07597 21600.00 16800.00 0.00 0.07675 21800.00 16800.00 0.00 0.07761 22000.00 16800.00 0.00 0.07776 21600.00 17000.00 0.00 0.07855 21800.00 17000.00 0.00 0.07939 22000.00 17000.00 0.00 0.07947 21600.00 17200.00 0.00 0.08021 21800.00 17200.00 0.00 0.08098 21600.00 17400.00 0.00 0.08186 21800.00 17400.00 0.00 0.08257 21600.00 17600.00 0.00 0.08348 21800.00 17600.00 0.00 0.08415 21600.00 17800.00 0.00 0.08525 21800.00 17800.00 0.00 0.08588 21600.00 18000.00 0.00 0.08708 21800.00 18000.00 0.00 0.08768 21600.00 18200.00 0.00 0.08903 21800.00 18200.00 0.00 0.08960 21600.00 18400.00 0.00 0.09107 21800.00 18400.00 0.00 0.09160 21600.00 18600.00 0.00 0.09312 21800.00 18600.00 0.00 0.09361 21600.00 18800.00 0.00 0.09523 21800.00 18800.00 0.00 0.09569 21600.00 19000.00 0.00 0.09739 21800.00 19000.00 0.00 0.09782 21600.00 19200.00 0.00 0.09973 21800.00 19200.00 0.00 0.10010 21600.00 19400.00 0.00 0.10200 21800.00 19400.00 0.00 0.10227 21400.00 19600.00 0.00 0.10248 21600.00 19600.00 0.00 0.10442 21800.00 19600.00 0.00 0.10458 21400.00 19800.00 0.00 0.10499 21600.00 19800.00 0.00 0.10686 21800.00 19800.00 0.00 0.10688 21400.00 20000.00 0.00 0.10765 21600.00 20000.00 0.00 0.10945 21400.00 20200.00 0.00 0.11056 21600.00 20200.00 0.00 0.11230 21400.00 20400.00 0.00 0.11341 21600.00 20400.00 0.00 0.11506 21400.00 20600.00 0.00 0.11656 21600.00 20600.00 0.00 0.11808 21400.00 20800.00 0.00 0.11958 21600.00 20800.00 0.00 0.12091 21400.00 21000.00 0.00 0.12272 21600.00 21000.00 0.00 0.12375 21400.00 21200.00 0.00 0.12556 21600.00 21200.00 0.00 0.12616 21400.00 21400.00 0.00 0.12789 21400.00 21600.00 0.00 0.12868 21400.00 21600.00 5.00 0.12876 21400.00 21600.00 10.00 0.12883 21400.00 21600.00 15.00 0.12887 21400.00 21600.00 20.00 0.12889 21400.00 21600.00 25.00 0.12890 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21433.15 21557.86 23.09 0.12898 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/576.ctf NX, NY, NZ: 512 512 1 MODE : real