CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/573.mrc Output diagnostic file name Micrographs_0601/573.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/573.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2168.000 8646.000 Mean, RMS : 4526.122 223.9707 TITLE 1: emi2mrc: 573.emi Mon Jan 06 19:04:44 2014 TITLE 2: kV 300, Mag 78000x, Def 23960, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -21.536, Y 217.136, Z -42.031, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01450 10200.00 10000.00 0.00 0.01570 10400.00 10000.00 0.00 0.01681 10600.00 10000.00 0.00 0.01791 10800.00 10000.00 0.00 0.01894 11000.00 10000.00 0.00 0.02006 11200.00 10000.00 0.00 0.02125 11400.00 10000.00 0.00 0.02261 11600.00 10000.00 0.00 0.02414 11800.00 10000.00 0.00 0.02579 12000.00 10000.00 0.00 0.02761 12200.00 10000.00 0.00 0.02960 12400.00 10000.00 0.00 0.03165 12600.00 10000.00 0.00 0.03361 12800.00 10000.00 0.00 0.03578 13000.00 10000.00 0.00 0.03801 13200.00 10000.00 0.00 0.04033 13400.00 10000.00 0.00 0.04276 13600.00 10000.00 0.00 0.04516 13800.00 10000.00 0.00 0.04769 14000.00 10000.00 0.00 0.05028 14200.00 10000.00 0.00 0.05301 14400.00 10000.00 0.00 0.05571 14600.00 10000.00 0.00 0.05856 14800.00 10000.00 0.00 0.06170 15000.00 10000.00 0.00 0.06501 15200.00 10000.00 0.00 0.06852 15400.00 10000.00 0.00 0.07204 15600.00 10000.00 0.00 0.07543 15800.00 10000.00 0.00 0.07749 16000.00 10000.00 0.00 0.07821 16200.00 10000.00 0.00 0.07845 15800.00 10200.00 0.00 0.07900 16000.00 10200.00 0.00 0.07971 16200.00 10200.00 0.00 0.07993 15800.00 10400.00 0.00 0.08057 16000.00 10400.00 0.00 0.08126 16200.00 10400.00 0.00 0.08144 15800.00 10600.00 0.00 0.08221 16000.00 10600.00 0.00 0.08288 16200.00 10600.00 0.00 0.08303 15800.00 10800.00 0.00 0.08400 16000.00 10800.00 0.00 0.08466 16200.00 10800.00 0.00 0.08480 15800.00 11000.00 0.00 0.08605 16000.00 11000.00 0.00 0.08670 16200.00 11000.00 0.00 0.08679 15800.00 11200.00 0.00 0.08793 16000.00 11200.00 0.00 0.08853 16200.00 11200.00 0.00 0.08856 15800.00 11400.00 0.00 0.08993 16000.00 11400.00 0.00 0.09048 15800.00 11600.00 0.00 0.09192 16000.00 11600.00 0.00 0.09246 15800.00 11800.00 0.00 0.09424 16000.00 11800.00 0.00 0.09478 15800.00 12000.00 0.00 0.09680 16000.00 12000.00 0.00 0.09730 15800.00 12200.00 0.00 0.09929 16000.00 12200.00 0.00 0.09976 15600.00 12400.00 0.00 0.10000 15800.00 12400.00 0.00 0.10208 16000.00 12400.00 0.00 0.10252 15600.00 12600.00 0.00 0.10290 15800.00 12600.00 0.00 0.10500 16000.00 12600.00 0.00 0.10538 15600.00 12800.00 0.00 0.10607 15800.00 12800.00 0.00 0.10816 16000.00 12800.00 0.00 0.10843 15600.00 13000.00 0.00 0.10916 15800.00 13000.00 0.00 0.11118 16000.00 13000.00 0.00 0.11132 15600.00 13200.00 0.00 0.11226 15800.00 13200.00 0.00 0.11418 15600.00 13400.00 0.00 0.11532 15800.00 13400.00 0.00 0.11717 15600.00 13600.00 0.00 0.11864 15800.00 13600.00 0.00 0.12046 15600.00 13800.00 0.00 0.12210 15800.00 13800.00 0.00 0.12385 15600.00 14000.00 0.00 0.12543 15800.00 14000.00 0.00 0.12712 15600.00 14200.00 0.00 0.12918 15800.00 14200.00 0.00 0.13078 15600.00 14400.00 0.00 0.13293 15800.00 14400.00 0.00 0.13438 15600.00 14600.00 0.00 0.13724 15800.00 14600.00 0.00 0.13845 15600.00 14800.00 0.00 0.14163 15800.00 14800.00 0.00 0.14251 15600.00 15000.00 0.00 0.14624 15800.00 15000.00 0.00 0.14666 15400.00 15200.00 0.00 0.14696 15600.00 15200.00 0.00 0.15078 15400.00 15400.00 0.00 0.15134 15600.00 15400.00 0.00 0.15489 15400.00 15600.00 0.00 0.15534 15600.00 15600.00 0.00 0.15773 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15579.93 15638.89 22.20 0.15784 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/573.ctf NX, NY, NZ: 512 512 1 MODE : real