CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/571.mrc Output diagnostic file name Micrographs_0601/571.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/571.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2064.000 8108.000 Mean, RMS : 4533.541 217.2980 TITLE 1: emi2mrc: 571.emi Mon Jan 06 19:02:26 2014 TITLE 2: kV 300, Mag 78000x, Def 21820, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X -24.773, Y 221.242, Z -42.031, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01747 10200.00 10000.00 0.00 0.01873 10400.00 10000.00 0.00 0.01981 10600.00 10000.00 0.00 0.02110 10800.00 10000.00 0.00 0.02252 11000.00 10000.00 0.00 0.02415 11200.00 10000.00 0.00 0.02588 11400.00 10000.00 0.00 0.02767 11600.00 10000.00 0.00 0.02963 11800.00 10000.00 0.00 0.03167 12000.00 10000.00 0.00 0.03395 12200.00 10000.00 0.00 0.03612 12400.00 10000.00 0.00 0.03842 12600.00 10000.00 0.00 0.04096 12800.00 10000.00 0.00 0.04360 13000.00 10000.00 0.00 0.04628 13200.00 10000.00 0.00 0.04903 13400.00 10000.00 0.00 0.05199 13600.00 10000.00 0.00 0.05498 13800.00 10000.00 0.00 0.05803 14000.00 10000.00 0.00 0.06112 14200.00 10000.00 0.00 0.06446 14400.00 10000.00 0.00 0.06804 14600.00 10000.00 0.00 0.07191 14800.00 10000.00 0.00 0.07588 15000.00 10000.00 0.00 0.07889 15200.00 10000.00 0.00 0.08039 15400.00 10000.00 0.00 0.08072 15000.00 10200.00 0.00 0.08088 15200.00 10200.00 0.00 0.08237 15400.00 10200.00 0.00 0.08265 15000.00 10400.00 0.00 0.08297 15200.00 10400.00 0.00 0.08444 15400.00 10400.00 0.00 0.08463 15000.00 10600.00 0.00 0.08523 15200.00 10600.00 0.00 0.08665 15400.00 10600.00 0.00 0.08680 15000.00 10800.00 0.00 0.08763 15200.00 10800.00 0.00 0.08903 15400.00 10800.00 0.00 0.08917 15000.00 11000.00 0.00 0.09013 15200.00 11000.00 0.00 0.09151 15400.00 11000.00 0.00 0.09163 15000.00 11200.00 0.00 0.09251 15200.00 11200.00 0.00 0.09391 15400.00 11200.00 0.00 0.09399 15000.00 11400.00 0.00 0.09538 15200.00 11400.00 0.00 0.09674 15000.00 11600.00 0.00 0.09831 15200.00 11600.00 0.00 0.09958 15000.00 11800.00 0.00 0.10126 15200.00 11800.00 0.00 0.10245 15000.00 12000.00 0.00 0.10434 15200.00 12000.00 0.00 0.10551 15000.00 12200.00 0.00 0.10745 15200.00 12200.00 0.00 0.10862 15000.00 12400.00 0.00 0.11076 15200.00 12400.00 0.00 0.11188 15000.00 12600.00 0.00 0.11408 15200.00 12600.00 0.00 0.11506 15000.00 12800.00 0.00 0.11759 15200.00 12800.00 0.00 0.11840 15000.00 13000.00 0.00 0.12116 15200.00 13000.00 0.00 0.12182 14800.00 13200.00 0.00 0.12210 15000.00 13200.00 0.00 0.12514 15200.00 13200.00 0.00 0.12562 14800.00 13400.00 0.00 0.12609 15000.00 13400.00 0.00 0.12901 15200.00 13400.00 0.00 0.12925 14800.00 13600.00 0.00 0.13005 15000.00 13600.00 0.00 0.13280 14800.00 13800.00 0.00 0.13412 15000.00 13800.00 0.00 0.13668 14800.00 14000.00 0.00 0.13834 15000.00 14000.00 0.00 0.14074 14800.00 14200.00 0.00 0.14333 15000.00 14200.00 0.00 0.14546 14800.00 14400.00 0.00 0.14784 15000.00 14400.00 0.00 0.14951 14800.00 14600.00 0.00 0.15241 15000.00 14600.00 0.00 0.15313 14800.00 14800.00 0.00 0.15560 14800.00 15000.00 0.00 0.15577 14800.00 15000.00 5.00 0.15584 14800.00 15000.00 10.00 0.15589 14800.00 15000.00 15.00 0.15593 14800.00 15000.00 20.00 0.15595 14800.00 15000.00 25.00 0.15596 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14821.58 14924.55 26.77 0.15609 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/571.ctf NX, NY, NZ: 512 512 1 MODE : real