CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/569.mrc Output diagnostic file name Micrographs_0601/569.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/569.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2390.000 10074.00 Mean, RMS : 4516.601 209.5558 TITLE 1: emi2mrc: 569.emi Mon Jan 06 18:49:17 2014 TITLE 2: kV 300, Mag 78000x, Def 22480, Spot 4, Int 55.04%, Obj 88.08%, Dif 73.67% TITLE 3: X 1.911, Y 273.130, Z -41.066, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02049 10200.00 10000.00 0.00 0.02213 10400.00 10000.00 0.00 0.02353 10600.00 10000.00 0.00 0.02533 10800.00 10000.00 0.00 0.02723 11000.00 10000.00 0.00 0.02935 11200.00 10000.00 0.00 0.03145 11400.00 10000.00 0.00 0.03336 11600.00 10000.00 0.00 0.03569 11800.00 10000.00 0.00 0.03827 12000.00 10000.00 0.00 0.04117 12200.00 10000.00 0.00 0.04436 12400.00 10000.00 0.00 0.04760 12600.00 10000.00 0.00 0.05095 12800.00 10000.00 0.00 0.05448 13000.00 10000.00 0.00 0.05783 13200.00 10000.00 0.00 0.06103 13400.00 10000.00 0.00 0.06437 13600.00 10000.00 0.00 0.06776 13800.00 10000.00 0.00 0.07163 14000.00 10000.00 0.00 0.07592 14200.00 10000.00 0.00 0.08029 14400.00 10000.00 0.00 0.08441 14600.00 10000.00 0.00 0.08737 14800.00 10000.00 0.00 0.08802 14600.00 10200.00 0.00 0.08928 14800.00 10200.00 0.00 0.08982 14600.00 10400.00 0.00 0.09114 14800.00 10400.00 0.00 0.09158 14600.00 10600.00 0.00 0.09311 14800.00 10600.00 0.00 0.09343 14600.00 10800.00 0.00 0.09504 14800.00 10800.00 0.00 0.09518 14600.00 11000.00 0.00 0.09708 14600.00 11200.00 0.00 0.09892 14600.00 11400.00 0.00 0.10075 14600.00 11600.00 0.00 0.10233 14600.00 11800.00 0.00 0.10407 14600.00 12000.00 0.00 0.10601 14600.00 12200.00 0.00 0.10774 14600.00 12400.00 0.00 0.10948 14600.00 12600.00 0.00 0.11127 14400.00 12800.00 0.00 0.11147 14600.00 12800.00 0.00 0.11318 14600.00 13000.00 0.00 0.11463 14600.00 13200.00 0.00 0.11600 14400.00 13400.00 0.00 0.11604 14600.00 13400.00 0.00 0.11734 14400.00 13600.00 0.00 0.11761 14600.00 13600.00 0.00 0.11875 14400.00 13800.00 0.00 0.11925 14600.00 13800.00 0.00 0.12025 14400.00 14000.00 0.00 0.12071 14600.00 14000.00 0.00 0.12154 14400.00 14200.00 0.00 0.12248 14600.00 14200.00 0.00 0.12290 14400.00 14400.00 0.00 0.12361 14600.00 14400.00 0.00 0.12366 14400.00 14600.00 0.00 0.12434 14400.00 14600.00 5.00 0.12435 14400.00 14600.00 10.00 0.12436 14400.00 14600.00 15.00 0.12438 14400.00 14600.00 20.00 0.12439 14400.00 14600.00 25.00 0.12440 14400.00 14600.00 30.00 0.12440 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14480.59 14600.00 10.51 0.12466 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/569.ctf NX, NY, NZ: 512 512 1 MODE : real