CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/568.mrc Output diagnostic file name Micrographs_0601/568.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/568.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2464.000 8680.000 Mean, RMS : 4534.438 219.1640 TITLE 1: emi2mrc: 568.emi Mon Jan 06 19:00:39 2014 TITLE 2: kV 300, Mag 78000x, Def 21820, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X -27.951, Y 225.669, Z -42.031, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01237 10200.00 10000.00 0.00 -0.01231 10400.00 10000.00 0.00 -0.01211 10600.00 10000.00 0.00 -0.01188 10800.00 10000.00 0.00 -0.01175 11200.00 10000.00 0.00 -0.01163 11400.00 10000.00 0.00 -0.01128 11600.00 10000.00 0.00 -0.01103 11800.00 10000.00 0.00 -0.01075 12000.00 10000.00 0.00 -0.01037 12200.00 10000.00 0.00 -0.01005 12400.00 10000.00 0.00 -0.00983 12600.00 10000.00 0.00 -0.00959 12800.00 10000.00 0.00 -0.00938 13000.00 10000.00 0.00 -0.00904 13200.00 10000.00 0.00 -0.00853 13400.00 10000.00 0.00 -0.00802 13600.00 10000.00 0.00 -0.00751 13800.00 10000.00 0.00 -0.00696 14000.00 10000.00 0.00 -0.00637 14200.00 10000.00 0.00 -0.00583 14400.00 10000.00 0.00 -0.00518 14600.00 10000.00 0.00 -0.00441 14800.00 10000.00 0.00 -0.00368 15000.00 10000.00 0.00 -0.00284 15200.00 10000.00 0.00 -0.00194 15400.00 10000.00 0.00 -0.00104 15600.00 10000.00 0.00 -0.00007 15800.00 10000.00 0.00 0.00091 16000.00 10000.00 0.00 0.00199 16200.00 10000.00 0.00 0.00310 16400.00 10000.00 0.00 0.00416 16600.00 10000.00 0.00 0.00533 16800.00 10000.00 0.00 0.00649 17000.00 10000.00 0.00 0.00747 17200.00 10000.00 0.00 0.00841 17400.00 10000.00 0.00 0.00945 17600.00 10000.00 0.00 0.01054 17800.00 10000.00 0.00 0.01163 18000.00 10000.00 0.00 0.01281 18200.00 10000.00 0.00 0.01408 18400.00 10000.00 0.00 0.01536 18600.00 10000.00 0.00 0.01667 18800.00 10000.00 0.00 0.01812 19000.00 10000.00 0.00 0.01963 19200.00 10000.00 0.00 0.02118 19400.00 10000.00 0.00 0.02264 19600.00 10000.00 0.00 0.02410 19800.00 10000.00 0.00 0.02574 20000.00 10000.00 0.00 0.02749 20200.00 10000.00 0.00 0.02937 20400.00 10000.00 0.00 0.03143 20600.00 10000.00 0.00 0.03361 20800.00 10000.00 0.00 0.03576 21000.00 10000.00 0.00 0.03795 21200.00 10000.00 0.00 0.03990 21400.00 10000.00 0.00 0.04111 21600.00 10000.00 0.00 0.04171 21800.00 10000.00 0.00 0.04196 22000.00 10000.00 0.00 0.04199 21600.00 10200.00 0.00 0.04243 21800.00 10200.00 0.00 0.04264 21600.00 10400.00 0.00 0.04297 21800.00 10400.00 0.00 0.04317 22000.00 10400.00 0.00 0.04319 21600.00 10600.00 0.00 0.04356 21800.00 10600.00 0.00 0.04379 22000.00 10600.00 0.00 0.04385 21600.00 10800.00 0.00 0.04416 21800.00 10800.00 0.00 0.04440 22000.00 10800.00 0.00 0.04447 21600.00 11000.00 0.00 0.04481 21800.00 11000.00 0.00 0.04506 22000.00 11000.00 0.00 0.04514 21600.00 11200.00 0.00 0.04545 21800.00 11200.00 0.00 0.04574 22000.00 11200.00 0.00 0.04582 21600.00 11400.00 0.00 0.04612 21800.00 11400.00 0.00 0.04640 22000.00 11400.00 0.00 0.04645 21600.00 11600.00 0.00 0.04668 21800.00 11600.00 0.00 0.04696 22000.00 11600.00 0.00 0.04698 21600.00 11800.00 0.00 0.04730 21800.00 11800.00 0.00 0.04758 22000.00 11800.00 0.00 0.04760 21600.00 12000.00 0.00 0.04810 21800.00 12000.00 0.00 0.04838 22000.00 12000.00 0.00 0.04842 21400.00 12200.00 0.00 0.04846 21600.00 12200.00 0.00 0.04914 21800.00 12200.00 0.00 0.04943 22000.00 12200.00 0.00 0.04949 21400.00 12400.00 0.00 0.04960 21600.00 12400.00 0.00 0.05030 21800.00 12400.00 0.00 0.05058 22000.00 12400.00 0.00 0.05064 21600.00 12600.00 0.00 0.05128 21800.00 12600.00 0.00 0.05156 22000.00 12600.00 0.00 0.05161 21400.00 12800.00 0.00 0.05170 21600.00 12800.00 0.00 0.05239 21800.00 12800.00 0.00 0.05268 22000.00 12800.00 0.00 0.05274 21400.00 13000.00 0.00 0.05288 21600.00 13000.00 0.00 0.05357 21800.00 13000.00 0.00 0.05385 22000.00 13000.00 0.00 0.05389 21400.00 13200.00 0.00 0.05407 21600.00 13200.00 0.00 0.05471 21800.00 13200.00 0.00 0.05497 21400.00 13400.00 0.00 0.05526 21600.00 13400.00 0.00 0.05586 21800.00 13400.00 0.00 0.05609 21400.00 13600.00 0.00 0.05641 21600.00 13600.00 0.00 0.05699 21800.00 13600.00 0.00 0.05721 21400.00 13800.00 0.00 0.05760 21600.00 13800.00 0.00 0.05815 21800.00 13800.00 0.00 0.05834 21400.00 14000.00 0.00 0.05880 21600.00 14000.00 0.00 0.05934 21800.00 14000.00 0.00 0.05950 21400.00 14200.00 0.00 0.06004 21600.00 14200.00 0.00 0.06060 21800.00 14200.00 0.00 0.06073 21400.00 14400.00 0.00 0.06128 21600.00 14400.00 0.00 0.06181 21800.00 14400.00 0.00 0.06190 21400.00 14600.00 0.00 0.06265 21600.00 14600.00 0.00 0.06316 21800.00 14600.00 0.00 0.06325 21400.00 14800.00 0.00 0.06403 21600.00 14800.00 0.00 0.06455 21800.00 14800.00 0.00 0.06466 21400.00 15000.00 0.00 0.06553 21600.00 15000.00 0.00 0.06606 21800.00 15000.00 0.00 0.06614 21400.00 15200.00 0.00 0.06709 21600.00 15200.00 0.00 0.06756 21800.00 15200.00 0.00 0.06757 21400.00 15400.00 0.00 0.06845 21600.00 15400.00 0.00 0.06886 21400.00 15600.00 0.00 0.06985 21600.00 15600.00 0.00 0.07027 21800.00 15600.00 0.00 0.07030 21400.00 15800.00 0.00 0.07130 21600.00 15800.00 0.00 0.07178 21800.00 15800.00 0.00 0.07184 21400.00 16000.00 0.00 0.07292 21600.00 16000.00 0.00 0.07346 21800.00 16000.00 0.00 0.07347 21200.00 16200.00 0.00 0.07349 21400.00 16200.00 0.00 0.07463 21600.00 16200.00 0.00 0.07511 21200.00 16400.00 0.00 0.07517 21400.00 16400.00 0.00 0.07624 21600.00 16400.00 0.00 0.07666 21200.00 16600.00 0.00 0.07672 21400.00 16600.00 0.00 0.07777 21600.00 16600.00 0.00 0.07818 21200.00 16800.00 0.00 0.07848 21400.00 16800.00 0.00 0.07953 21600.00 16800.00 0.00 0.07996 21200.00 17000.00 0.00 0.08043 21400.00 17000.00 0.00 0.08147 21600.00 17000.00 0.00 0.08187 21200.00 17200.00 0.00 0.08218 21400.00 17200.00 0.00 0.08320 21600.00 17200.00 0.00 0.08358 21200.00 17400.00 0.00 0.08408 21400.00 17400.00 0.00 0.08511 21600.00 17400.00 0.00 0.08549 21200.00 17600.00 0.00 0.08616 21400.00 17600.00 0.00 0.08721 21600.00 17600.00 0.00 0.08759 21200.00 17800.00 0.00 0.08828 21400.00 17800.00 0.00 0.08934 21600.00 17800.00 0.00 0.08965 21200.00 18000.00 0.00 0.09033 21400.00 18000.00 0.00 0.09134 21600.00 18000.00 0.00 0.09154 21200.00 18200.00 0.00 0.09234 21400.00 18200.00 0.00 0.09327 21600.00 18200.00 0.00 0.09335 21200.00 18400.00 0.00 0.09425 21400.00 18400.00 0.00 0.09511 21600.00 18400.00 0.00 0.09511 21200.00 18600.00 0.00 0.09622 21400.00 18600.00 0.00 0.09699 21200.00 18800.00 0.00 0.09828 21400.00 18800.00 0.00 0.09896 21200.00 19000.00 0.00 0.10027 21400.00 19000.00 0.00 0.10092 21200.00 19200.00 0.00 0.10256 21400.00 19200.00 0.00 0.10319 21200.00 19400.00 0.00 0.10488 21400.00 19400.00 0.00 0.10544 21000.00 19600.00 0.00 0.10550 21200.00 19600.00 0.00 0.10737 21400.00 19600.00 0.00 0.10781 21000.00 19800.00 0.00 0.10807 21200.00 19800.00 0.00 0.10987 21400.00 19800.00 0.00 0.11008 21000.00 20000.00 0.00 0.11057 21200.00 20000.00 0.00 0.11223 21000.00 20200.00 0.00 0.11313 21200.00 20200.00 0.00 0.11463 21000.00 20400.00 0.00 0.11558 21200.00 20400.00 0.00 0.11686 21000.00 20600.00 0.00 0.11810 21200.00 20600.00 0.00 0.11908 21000.00 20800.00 0.00 0.11996 21200.00 20800.00 0.00 0.12063 21000.00 21000.00 0.00 0.12154 21200.00 21000.00 0.00 0.12177 21000.00 21200.00 0.00 0.12232 21000.00 21200.00 5.00 0.12238 21000.00 21200.00 10.00 0.12242 21000.00 21200.00 15.00 0.12245 21000.00 21200.00 20.00 0.12247 21000.00 21200.00 25.00 0.12248 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21059.44 21188.70 25.00 0.12258 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/568.ctf NX, NY, NZ: 512 512 1 MODE : real