CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/567.mrc Output diagnostic file name Micrographs_0601/567.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/567.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2070.000 8050.000 Mean, RMS : 4525.357 223.6809 TITLE 1: emi2mrc: 567.emi Mon Jan 06 18:59:19 2014 TITLE 2: kV 300, Mag 78000x, Def 21990, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -29.542, Y 227.760, Z -42.031, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02374 14800.00 10000.00 0.00 -0.02369 15000.00 10000.00 0.00 -0.02337 15200.00 10000.00 0.00 -0.02296 15400.00 10000.00 0.00 -0.02235 15600.00 10000.00 0.00 -0.02176 15800.00 10000.00 0.00 -0.02122 16000.00 10000.00 0.00 -0.02067 16200.00 10000.00 0.00 -0.02017 16400.00 10000.00 0.00 -0.01960 16600.00 10000.00 0.00 -0.01902 16800.00 10000.00 0.00 -0.01838 17000.00 10000.00 0.00 -0.01775 17200.00 10000.00 0.00 -0.01712 17400.00 10000.00 0.00 -0.01652 17600.00 10000.00 0.00 -0.01600 17800.00 10000.00 0.00 -0.01534 18000.00 10000.00 0.00 -0.01465 18200.00 10000.00 0.00 -0.01403 18400.00 10000.00 0.00 -0.01328 18600.00 10000.00 0.00 -0.01234 18800.00 10000.00 0.00 -0.01141 19000.00 10000.00 0.00 -0.01045 19200.00 10000.00 0.00 -0.00948 19400.00 10000.00 0.00 -0.00867 19600.00 10000.00 0.00 -0.00794 19800.00 10000.00 0.00 -0.00731 20000.00 10000.00 0.00 -0.00668 20200.00 10000.00 0.00 -0.00575 20400.00 10000.00 0.00 -0.00477 20600.00 10000.00 0.00 -0.00397 20800.00 10000.00 0.00 -0.00320 21000.00 10000.00 0.00 -0.00229 21200.00 10000.00 0.00 -0.00128 21400.00 10000.00 0.00 -0.00024 21600.00 10000.00 0.00 0.00072 21800.00 10000.00 0.00 0.00151 22000.00 10000.00 0.00 0.00229 22200.00 10000.00 0.00 0.00315 22400.00 10000.00 0.00 0.00402 22600.00 10000.00 0.00 0.00504 22800.00 10000.00 0.00 0.00624 23000.00 10000.00 0.00 0.00742 23200.00 10000.00 0.00 0.00861 23400.00 10000.00 0.00 0.00993 23600.00 10000.00 0.00 0.01133 23800.00 10000.00 0.00 0.01271 24000.00 10000.00 0.00 0.01406 24200.00 10000.00 0.00 0.01543 24400.00 10000.00 0.00 0.01669 24600.00 10000.00 0.00 0.01799 24800.00 10000.00 0.00 0.01931 25000.00 10000.00 0.00 0.02060 25200.00 10000.00 0.00 0.02172 25400.00 10000.00 0.00 0.02234 25600.00 10000.00 0.00 0.02288 25800.00 10000.00 0.00 0.02324 25600.00 10200.00 0.00 0.02327 25800.00 10200.00 0.00 0.02363 25600.00 10400.00 0.00 0.02373 25800.00 10400.00 0.00 0.02407 25600.00 10600.00 0.00 0.02411 25800.00 10600.00 0.00 0.02439 25600.00 10800.00 0.00 0.02441 25800.00 10800.00 0.00 0.02464 25600.00 11000.00 0.00 0.02471 25800.00 11000.00 0.00 0.02493 25600.00 11200.00 0.00 0.02503 25800.00 11200.00 0.00 0.02526 25600.00 11400.00 0.00 0.02549 25800.00 11400.00 0.00 0.02568 25600.00 11600.00 0.00 0.02589 25800.00 11600.00 0.00 0.02603 25600.00 11800.00 0.00 0.02635 25800.00 11800.00 0.00 0.02646 25600.00 12000.00 0.00 0.02692 25800.00 12000.00 0.00 0.02700 25600.00 12200.00 0.00 0.02743 25800.00 12200.00 0.00 0.02750 25600.00 12400.00 0.00 0.02801 25800.00 12400.00 0.00 0.02811 25600.00 12600.00 0.00 0.02851 25800.00 12600.00 0.00 0.02861 25600.00 12800.00 0.00 0.02898 25800.00 12800.00 0.00 0.02906 25400.00 13000.00 0.00 0.02907 25600.00 13000.00 0.00 0.02955 25800.00 13000.00 0.00 0.02963 25400.00 13200.00 0.00 0.02967 25600.00 13200.00 0.00 0.03012 25800.00 13200.00 0.00 0.03021 26000.00 13200.00 0.00 0.03024 25400.00 13400.00 0.00 0.03034 25600.00 13400.00 0.00 0.03073 25800.00 13400.00 0.00 0.03085 26000.00 13400.00 0.00 0.03091 25400.00 13600.00 0.00 0.03106 25600.00 13600.00 0.00 0.03143 25800.00 13600.00 0.00 0.03160 26000.00 13600.00 0.00 0.03166 25400.00 13800.00 0.00 0.03173 25600.00 13800.00 0.00 0.03215 25800.00 13800.00 0.00 0.03238 26000.00 13800.00 0.00 0.03241 25600.00 14000.00 0.00 0.03287 25800.00 14000.00 0.00 0.03308 25400.00 14200.00 0.00 0.03310 25600.00 14200.00 0.00 0.03354 25800.00 14200.00 0.00 0.03367 25400.00 14400.00 0.00 0.03385 25600.00 14400.00 0.00 0.03423 25800.00 14400.00 0.00 0.03430 25400.00 14600.00 0.00 0.03470 25600.00 14600.00 0.00 0.03503 25800.00 14600.00 0.00 0.03510 25400.00 14800.00 0.00 0.03548 25600.00 14800.00 0.00 0.03580 25800.00 14800.00 0.00 0.03588 25400.00 15000.00 0.00 0.03630 25600.00 15000.00 0.00 0.03662 25800.00 15000.00 0.00 0.03672 25400.00 15200.00 0.00 0.03720 25600.00 15200.00 0.00 0.03753 25800.00 15200.00 0.00 0.03765 25400.00 15400.00 0.00 0.03801 25600.00 15400.00 0.00 0.03832 25800.00 15400.00 0.00 0.03846 25400.00 15600.00 0.00 0.03872 25600.00 15600.00 0.00 0.03905 25800.00 15600.00 0.00 0.03922 25400.00 15800.00 0.00 0.03948 25600.00 15800.00 0.00 0.03984 25800.00 15800.00 0.00 0.04003 25400.00 16000.00 0.00 0.04037 25600.00 16000.00 0.00 0.04076 25800.00 16000.00 0.00 0.04092 25400.00 16200.00 0.00 0.04136 25600.00 16200.00 0.00 0.04177 25800.00 16200.00 0.00 0.04188 25400.00 16400.00 0.00 0.04244 25600.00 16400.00 0.00 0.04281 25800.00 16400.00 0.00 0.04287 25400.00 16600.00 0.00 0.04349 25600.00 16600.00 0.00 0.04380 25800.00 16600.00 0.00 0.04383 25200.00 16800.00 0.00 0.04388 25400.00 16800.00 0.00 0.04451 25600.00 16800.00 0.00 0.04481 25800.00 16800.00 0.00 0.04487 25200.00 17000.00 0.00 0.04497 25400.00 17000.00 0.00 0.04560 25600.00 17000.00 0.00 0.04594 25800.00 17000.00 0.00 0.04603 25400.00 17200.00 0.00 0.04666 25600.00 17200.00 0.00 0.04702 25800.00 17200.00 0.00 0.04712 25400.00 17400.00 0.00 0.04772 25600.00 17400.00 0.00 0.04807 25800.00 17400.00 0.00 0.04814 25400.00 17600.00 0.00 0.04879 25600.00 17600.00 0.00 0.04911 25800.00 17600.00 0.00 0.04914 25200.00 17800.00 0.00 0.04924 25400.00 17800.00 0.00 0.04991 25600.00 17800.00 0.00 0.05022 25200.00 18000.00 0.00 0.05048 25400.00 18000.00 0.00 0.05113 25600.00 18000.00 0.00 0.05140 25200.00 18200.00 0.00 0.05173 25400.00 18200.00 0.00 0.05234 25600.00 18200.00 0.00 0.05254 25200.00 18400.00 0.00 0.05292 25400.00 18400.00 0.00 0.05349 25600.00 18400.00 0.00 0.05364 25200.00 18600.00 0.00 0.05417 25400.00 18600.00 0.00 0.05470 25600.00 18600.00 0.00 0.05478 25200.00 18800.00 0.00 0.05533 25400.00 18800.00 0.00 0.05579 25600.00 18800.00 0.00 0.05581 25200.00 19000.00 0.00 0.05629 25400.00 19000.00 0.00 0.05673 25600.00 19000.00 0.00 0.05675 25200.00 19200.00 0.00 0.05737 25400.00 19200.00 0.00 0.05781 25600.00 19200.00 0.00 0.05782 25200.00 19400.00 0.00 0.05846 25400.00 19400.00 0.00 0.05890 25600.00 19400.00 0.00 0.05892 25200.00 19600.00 0.00 0.05956 25400.00 19600.00 0.00 0.06002 25200.00 19800.00 0.00 0.06074 25400.00 19800.00 0.00 0.06119 25200.00 20000.00 0.00 0.06197 25400.00 20000.00 0.00 0.06238 25200.00 20200.00 0.00 0.06322 25400.00 20200.00 0.00 0.06358 25200.00 20400.00 0.00 0.06446 25400.00 20400.00 0.00 0.06477 25200.00 20600.00 0.00 0.06574 25400.00 20600.00 0.00 0.06601 25200.00 20800.00 0.00 0.06699 25400.00 20800.00 0.00 0.06730 25200.00 21000.00 0.00 0.06839 25400.00 21000.00 0.00 0.06871 25200.00 21200.00 0.00 0.06976 25400.00 21200.00 0.00 0.07004 25200.00 21400.00 0.00 0.07112 25400.00 21400.00 0.00 0.07134 25000.00 21600.00 0.00 0.07140 25200.00 21600.00 0.00 0.07253 25400.00 21600.00 0.00 0.07270 25000.00 21800.00 0.00 0.07282 25200.00 21800.00 0.00 0.07391 25400.00 21800.00 0.00 0.07406 25000.00 22000.00 0.00 0.07443 25200.00 22000.00 0.00 0.07550 25400.00 22000.00 0.00 0.07565 25000.00 22200.00 0.00 0.07617 25200.00 22200.00 0.00 0.07721 25400.00 22200.00 0.00 0.07733 25000.00 22400.00 0.00 0.07803 25200.00 22400.00 0.00 0.07904 25400.00 22400.00 0.00 0.07915 25000.00 22600.00 0.00 0.07999 25200.00 22600.00 0.00 0.08100 25400.00 22600.00 0.00 0.08111 25000.00 22800.00 0.00 0.08201 25200.00 22800.00 0.00 0.08302 25400.00 22800.00 0.00 0.08314 25000.00 23000.00 0.00 0.08409 25200.00 23000.00 0.00 0.08512 25400.00 23000.00 0.00 0.08519 25000.00 23200.00 0.00 0.08624 25200.00 23200.00 0.00 0.08725 25000.00 23400.00 0.00 0.08845 25200.00 23400.00 0.00 0.08942 25000.00 23600.00 0.00 0.09079 25200.00 23600.00 0.00 0.09168 25000.00 23800.00 0.00 0.09316 25200.00 23800.00 0.00 0.09394 25000.00 24000.00 0.00 0.09543 25200.00 24000.00 0.00 0.09609 24800.00 24200.00 0.00 0.09618 25000.00 24200.00 0.00 0.09790 25200.00 24200.00 0.00 0.09839 24800.00 24400.00 0.00 0.09854 25000.00 24400.00 0.00 0.10013 25200.00 24400.00 0.00 0.10040 24800.00 24600.00 0.00 0.10078 25000.00 24600.00 0.00 0.10220 24800.00 24800.00 0.00 0.10297 25000.00 24800.00 0.00 0.10401 24800.00 25000.00 0.00 0.10401 25000.00 25000.00 0.00 0.10465 24800.00 25200.00 30.00 0.10469 24800.00 25200.00 35.00 0.10475 24800.00 25200.00 40.00 0.10477 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24852.67 25097.76 42.21 0.10507 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/567.ctf NX, NY, NZ: 512 512 1 MODE : real