CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/562.mrc Output diagnostic file name Micrographs_0601/562.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/562.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2544.000 8644.000 Mean, RMS : 4540.472 224.1190 TITLE 1: emi2mrc: 562.emi Mon Jan 06 18:54:49 2014 TITLE 2: kV 300, Mag 78000x, Def 21990, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -29.203, Y 232.054, Z -42.031, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01992 14600.00 10000.00 0.00 -0.01969 14800.00 10000.00 0.00 -0.01941 15000.00 10000.00 0.00 -0.01904 15200.00 10000.00 0.00 -0.01856 15400.00 10000.00 0.00 -0.01799 15600.00 10000.00 0.00 -0.01736 15800.00 10000.00 0.00 -0.01671 16000.00 10000.00 0.00 -0.01601 16200.00 10000.00 0.00 -0.01535 16400.00 10000.00 0.00 -0.01479 16600.00 10000.00 0.00 -0.01424 16800.00 10000.00 0.00 -0.01362 17000.00 10000.00 0.00 -0.01296 17200.00 10000.00 0.00 -0.01225 17400.00 10000.00 0.00 -0.01150 17600.00 10000.00 0.00 -0.01065 17800.00 10000.00 0.00 -0.00971 18000.00 10000.00 0.00 -0.00882 18200.00 10000.00 0.00 -0.00784 18400.00 10000.00 0.00 -0.00679 18600.00 10000.00 0.00 -0.00588 18800.00 10000.00 0.00 -0.00496 19000.00 10000.00 0.00 -0.00393 19200.00 10000.00 0.00 -0.00293 19400.00 10000.00 0.00 -0.00206 19600.00 10000.00 0.00 -0.00135 19800.00 10000.00 0.00 -0.00051 20000.00 10000.00 0.00 0.00038 20200.00 10000.00 0.00 0.00128 20400.00 10000.00 0.00 0.00246 20600.00 10000.00 0.00 0.00360 20800.00 10000.00 0.00 0.00453 21000.00 10000.00 0.00 0.00539 21200.00 10000.00 0.00 0.00637 21400.00 10000.00 0.00 0.00750 21600.00 10000.00 0.00 0.00851 21800.00 10000.00 0.00 0.00948 22000.00 10000.00 0.00 0.01056 22200.00 10000.00 0.00 0.01175 22400.00 10000.00 0.00 0.01307 22600.00 10000.00 0.00 0.01430 22800.00 10000.00 0.00 0.01554 23000.00 10000.00 0.00 0.01690 23200.00 10000.00 0.00 0.01836 23400.00 10000.00 0.00 0.01995 23600.00 10000.00 0.00 0.02152 23800.00 10000.00 0.00 0.02321 24000.00 10000.00 0.00 0.02496 24200.00 10000.00 0.00 0.02632 24400.00 10000.00 0.00 0.02705 24600.00 10000.00 0.00 0.02728 24800.00 10000.00 0.00 0.02743 25000.00 10000.00 0.00 0.02759 25200.00 10000.00 0.00 0.02761 24800.00 10200.00 0.00 0.02764 25000.00 10200.00 0.00 0.02781 25200.00 10200.00 0.00 0.02784 25000.00 10400.00 0.00 0.02798 25200.00 10400.00 0.00 0.02799 24800.00 10600.00 0.00 0.02802 25000.00 10600.00 0.00 0.02819 25200.00 10600.00 0.00 0.02821 24800.00 10800.00 0.00 0.02840 25000.00 10800.00 0.00 0.02858 25200.00 10800.00 0.00 0.02861 24800.00 11000.00 0.00 0.02879 25000.00 11000.00 0.00 0.02896 25200.00 11000.00 0.00 0.02899 24800.00 11200.00 0.00 0.02922 25000.00 11200.00 0.00 0.02940 25200.00 11200.00 0.00 0.02947 25400.00 11200.00 0.00 0.02952 24800.00 11400.00 0.00 0.02978 25000.00 11400.00 0.00 0.03000 25200.00 11400.00 0.00 0.03010 25400.00 11400.00 0.00 0.03015 24800.00 11600.00 0.00 0.03026 25000.00 11600.00 0.00 0.03047 25200.00 11600.00 0.00 0.03055 25400.00 11600.00 0.00 0.03055 24800.00 11800.00 0.00 0.03065 25000.00 11800.00 0.00 0.03083 25200.00 11800.00 0.00 0.03087 24800.00 12000.00 0.00 0.03100 25000.00 12000.00 0.00 0.03116 25200.00 12000.00 0.00 0.03120 25400.00 12000.00 0.00 0.03121 24800.00 12200.00 0.00 0.03147 25000.00 12200.00 0.00 0.03161 25200.00 12200.00 0.00 0.03167 25400.00 12200.00 0.00 0.03169 24600.00 12400.00 0.00 0.03187 24800.00 12400.00 0.00 0.03214 25000.00 12400.00 0.00 0.03227 25200.00 12400.00 0.00 0.03233 25400.00 12400.00 0.00 0.03234 24600.00 12600.00 0.00 0.03261 24800.00 12600.00 0.00 0.03286 25000.00 12600.00 0.00 0.03299 25200.00 12600.00 0.00 0.03306 24600.00 12800.00 0.00 0.03344 24800.00 12800.00 0.00 0.03367 25000.00 12800.00 0.00 0.03378 25200.00 12800.00 0.00 0.03383 24400.00 13000.00 0.00 0.03384 24600.00 13000.00 0.00 0.03420 24800.00 13000.00 0.00 0.03439 25000.00 13000.00 0.00 0.03447 25200.00 13000.00 0.00 0.03451 24600.00 13200.00 0.00 0.03481 24800.00 13200.00 0.00 0.03499 25000.00 13200.00 0.00 0.03508 25200.00 13200.00 0.00 0.03515 24600.00 13400.00 0.00 0.03537 24800.00 13400.00 0.00 0.03557 25000.00 13400.00 0.00 0.03569 25200.00 13400.00 0.00 0.03580 24600.00 13600.00 0.00 0.03598 24800.00 13600.00 0.00 0.03621 25000.00 13600.00 0.00 0.03635 25200.00 13600.00 0.00 0.03647 24600.00 13800.00 0.00 0.03669 24800.00 13800.00 0.00 0.03693 25000.00 13800.00 0.00 0.03708 25200.00 13800.00 0.00 0.03719 24600.00 14000.00 0.00 0.03757 24800.00 14000.00 0.00 0.03779 25000.00 14000.00 0.00 0.03794 25200.00 14000.00 0.00 0.03804 24400.00 14200.00 0.00 0.03812 24600.00 14200.00 0.00 0.03846 24800.00 14200.00 0.00 0.03866 25000.00 14200.00 0.00 0.03883 25200.00 14200.00 0.00 0.03892 24600.00 14400.00 0.00 0.03920 24800.00 14400.00 0.00 0.03941 25000.00 14400.00 0.00 0.03959 25200.00 14400.00 0.00 0.03964 24400.00 14600.00 0.00 0.03968 24600.00 14600.00 0.00 0.03999 24800.00 14600.00 0.00 0.04022 25000.00 14600.00 0.00 0.04039 25200.00 14600.00 0.00 0.04039 24400.00 14800.00 0.00 0.04047 24600.00 14800.00 0.00 0.04080 24800.00 14800.00 0.00 0.04103 25000.00 14800.00 0.00 0.04118 24400.00 15000.00 0.00 0.04130 24600.00 15000.00 0.00 0.04163 24800.00 15000.00 0.00 0.04185 25000.00 15000.00 0.00 0.04198 24400.00 15200.00 0.00 0.04217 24600.00 15200.00 0.00 0.04247 24800.00 15200.00 0.00 0.04268 25000.00 15200.00 0.00 0.04278 24400.00 15400.00 0.00 0.04297 24600.00 15400.00 0.00 0.04327 24800.00 15400.00 0.00 0.04349 25000.00 15400.00 0.00 0.04361 24400.00 15600.00 0.00 0.04380 24600.00 15600.00 0.00 0.04415 24800.00 15600.00 0.00 0.04441 25000.00 15600.00 0.00 0.04452 24400.00 15800.00 0.00 0.04475 24600.00 15800.00 0.00 0.04512 24800.00 15800.00 0.00 0.04537 25000.00 15800.00 0.00 0.04544 24400.00 16000.00 0.00 0.04573 24600.00 16000.00 0.00 0.04610 24800.00 16000.00 0.00 0.04630 25000.00 16000.00 0.00 0.04635 24400.00 16200.00 0.00 0.04665 24600.00 16200.00 0.00 0.04701 24800.00 16200.00 0.00 0.04719 25000.00 16200.00 0.00 0.04725 24400.00 16400.00 0.00 0.04773 24600.00 16400.00 0.00 0.04807 24800.00 16400.00 0.00 0.04824 25000.00 16400.00 0.00 0.04829 24400.00 16600.00 0.00 0.04885 24600.00 16600.00 0.00 0.04916 24800.00 16600.00 0.00 0.04929 25000.00 16600.00 0.00 0.04930 24400.00 16800.00 0.00 0.05001 24600.00 16800.00 0.00 0.05026 24800.00 16800.00 0.00 0.05037 24400.00 17000.00 0.00 0.05117 24600.00 17000.00 0.00 0.05141 24800.00 17000.00 0.00 0.05153 24400.00 17200.00 0.00 0.05231 24600.00 17200.00 0.00 0.05255 24800.00 17200.00 0.00 0.05267 24400.00 17400.00 0.00 0.05359 24600.00 17400.00 0.00 0.05383 24800.00 17400.00 0.00 0.05393 24200.00 17600.00 0.00 0.05398 24400.00 17600.00 0.00 0.05490 24600.00 17600.00 0.00 0.05512 24800.00 17600.00 0.00 0.05520 24200.00 17800.00 0.00 0.05543 24400.00 17800.00 0.00 0.05633 24600.00 17800.00 0.00 0.05652 24800.00 17800.00 0.00 0.05656 24200.00 18000.00 0.00 0.05683 24400.00 18000.00 0.00 0.05771 24600.00 18000.00 0.00 0.05787 24800.00 18000.00 0.00 0.05791 24200.00 18200.00 0.00 0.05828 24400.00 18200.00 0.00 0.05915 24600.00 18200.00 0.00 0.05931 24800.00 18200.00 0.00 0.05938 24200.00 18400.00 0.00 0.05994 24400.00 18400.00 0.00 0.06078 24600.00 18400.00 0.00 0.06094 24800.00 18400.00 0.00 0.06100 24200.00 18600.00 0.00 0.06149 24400.00 18600.00 0.00 0.06228 24600.00 18600.00 0.00 0.06241 24800.00 18600.00 0.00 0.06244 24200.00 18800.00 0.00 0.06290 24400.00 18800.00 0.00 0.06366 24600.00 18800.00 0.00 0.06378 24200.00 19000.00 0.00 0.06425 24400.00 19000.00 0.00 0.06502 24600.00 19000.00 0.00 0.06513 24200.00 19200.00 0.00 0.06577 24400.00 19200.00 0.00 0.06652 24600.00 19200.00 0.00 0.06659 24200.00 19400.00 0.00 0.06731 24400.00 19400.00 0.00 0.06802 24600.00 19400.00 0.00 0.06805 24200.00 19600.00 0.00 0.06881 24400.00 19600.00 0.00 0.06949 24600.00 19600.00 0.00 0.06953 24200.00 19800.00 0.00 0.07032 24400.00 19800.00 0.00 0.07100 24600.00 19800.00 0.00 0.07104 24200.00 20000.00 0.00 0.07187 24400.00 20000.00 0.00 0.07252 24600.00 20000.00 0.00 0.07253 24200.00 20200.00 0.00 0.07341 24400.00 20200.00 0.00 0.07408 24600.00 20200.00 0.00 0.07408 24200.00 20400.00 0.00 0.07503 24400.00 20400.00 0.00 0.07573 24200.00 20600.00 0.00 0.07686 24400.00 20600.00 0.00 0.07750 24200.00 20800.00 0.00 0.07849 24400.00 20800.00 0.00 0.07906 24200.00 21000.00 0.00 0.08020 24400.00 21000.00 0.00 0.08077 24200.00 21200.00 0.00 0.08214 24400.00 21200.00 0.00 0.08272 24200.00 21400.00 0.00 0.08419 24400.00 21400.00 0.00 0.08472 24200.00 21600.00 0.00 0.08626 24400.00 21600.00 0.00 0.08672 24200.00 21800.00 0.00 0.08839 24400.00 21800.00 0.00 0.08879 24000.00 22000.00 0.00 0.08896 24200.00 22000.00 0.00 0.09061 24400.00 22000.00 0.00 0.09093 24000.00 22200.00 0.00 0.09118 24200.00 22200.00 0.00 0.09276 24400.00 22200.00 0.00 0.09293 24000.00 22400.00 0.00 0.09360 24200.00 22400.00 0.00 0.09507 24400.00 22400.00 0.00 0.09509 24000.00 22600.00 0.00 0.09598 24200.00 22600.00 0.00 0.09736 24000.00 22800.00 0.00 0.09850 24200.00 22800.00 0.00 0.09979 24000.00 23000.00 0.00 0.10113 24200.00 23000.00 0.00 0.10230 24000.00 23200.00 0.00 0.10385 24200.00 23200.00 0.00 0.10485 24000.00 23400.00 0.00 0.10664 24200.00 23400.00 0.00 0.10739 24000.00 23600.00 0.00 0.10919 24200.00 23600.00 0.00 0.10965 23800.00 23800.00 0.00 0.10989 24000.00 23800.00 0.00 0.11188 23800.00 24000.00 0.00 0.11211 24000.00 24000.00 0.00 0.11340 24000.00 24200.00 25.00 0.11349 24000.00 24200.00 30.00 0.11357 24000.00 24200.00 35.00 0.11362 24000.00 24200.00 40.00 0.11365 24000.00 24200.00 45.00 0.11366 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23937.66 24154.40 41.51 0.11390 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/562.ctf NX, NY, NZ: 512 512 1 MODE : real