CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/561.mrc Output diagnostic file name Micrographs_0601/561.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/561.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2324.000 7774.000 Mean, RMS : 4543.018 220.6083 TITLE 1: emi2mrc: 561.emi Mon Jan 06 18:53:51 2014 TITLE 2: kV 300, Mag 78000x, Def 21990, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -27.504, Y 229.987, Z -42.031, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02032 10800.00 10000.00 0.00 -0.02032 11200.00 10000.00 0.00 -0.02028 11400.00 10000.00 0.00 -0.02009 11600.00 10000.00 0.00 -0.01993 11800.00 10000.00 0.00 -0.01976 12000.00 10000.00 0.00 -0.01966 12200.00 10000.00 0.00 -0.01951 12400.00 10000.00 0.00 -0.01932 12600.00 10000.00 0.00 -0.01907 12800.00 10000.00 0.00 -0.01879 13000.00 10000.00 0.00 -0.01846 13200.00 10000.00 0.00 -0.01820 13400.00 10000.00 0.00 -0.01798 13600.00 10000.00 0.00 -0.01761 13800.00 10000.00 0.00 -0.01726 14000.00 10000.00 0.00 -0.01702 14200.00 10000.00 0.00 -0.01683 14400.00 10000.00 0.00 -0.01665 14600.00 10000.00 0.00 -0.01636 14800.00 10000.00 0.00 -0.01594 15000.00 10000.00 0.00 -0.01555 15200.00 10000.00 0.00 -0.01519 15400.00 10000.00 0.00 -0.01473 15600.00 10000.00 0.00 -0.01428 15800.00 10000.00 0.00 -0.01384 16000.00 10000.00 0.00 -0.01338 16200.00 10000.00 0.00 -0.01274 16400.00 10000.00 0.00 -0.01184 16600.00 10000.00 0.00 -0.01094 16800.00 10000.00 0.00 -0.01001 17000.00 10000.00 0.00 -0.00912 17200.00 10000.00 0.00 -0.00826 17400.00 10000.00 0.00 -0.00729 17600.00 10000.00 0.00 -0.00640 17800.00 10000.00 0.00 -0.00568 18000.00 10000.00 0.00 -0.00482 18200.00 10000.00 0.00 -0.00373 18400.00 10000.00 0.00 -0.00267 18600.00 10000.00 0.00 -0.00173 18800.00 10000.00 0.00 -0.00075 19000.00 10000.00 0.00 0.00032 19200.00 10000.00 0.00 0.00139 19400.00 10000.00 0.00 0.00240 19600.00 10000.00 0.00 0.00346 19800.00 10000.00 0.00 0.00456 20000.00 10000.00 0.00 0.00567 20200.00 10000.00 0.00 0.00691 20400.00 10000.00 0.00 0.00808 20600.00 10000.00 0.00 0.00914 20800.00 10000.00 0.00 0.01028 21000.00 10000.00 0.00 0.01144 21200.00 10000.00 0.00 0.01267 21400.00 10000.00 0.00 0.01406 21600.00 10000.00 0.00 0.01540 21800.00 10000.00 0.00 0.01671 22000.00 10000.00 0.00 0.01828 22200.00 10000.00 0.00 0.02002 22400.00 10000.00 0.00 0.02193 22600.00 10000.00 0.00 0.02386 22800.00 10000.00 0.00 0.02559 23000.00 10000.00 0.00 0.02736 23200.00 10000.00 0.00 0.02916 23400.00 10000.00 0.00 0.03078 23600.00 10000.00 0.00 0.03205 23800.00 10000.00 0.00 0.03276 24000.00 10000.00 0.00 0.03309 24200.00 10000.00 0.00 0.03327 24400.00 10000.00 0.00 0.03328 24000.00 10200.00 0.00 0.03349 24200.00 10200.00 0.00 0.03364 24000.00 10400.00 0.00 0.03380 24200.00 10400.00 0.00 0.03398 24400.00 10400.00 0.00 0.03400 24000.00 10600.00 0.00 0.03417 24200.00 10600.00 0.00 0.03439 24400.00 10600.00 0.00 0.03442 24000.00 10800.00 0.00 0.03465 24200.00 10800.00 0.00 0.03487 24400.00 10800.00 0.00 0.03488 24000.00 11000.00 0.00 0.03520 24200.00 11000.00 0.00 0.03542 24000.00 11200.00 0.00 0.03575 24200.00 11200.00 0.00 0.03599 24000.00 11400.00 0.00 0.03637 24200.00 11400.00 0.00 0.03659 24000.00 11600.00 0.00 0.03686 24200.00 11600.00 0.00 0.03702 24000.00 11800.00 0.00 0.03737 24200.00 11800.00 0.00 0.03751 23800.00 12000.00 0.00 0.03763 24000.00 12000.00 0.00 0.03797 24200.00 12000.00 0.00 0.03811 23800.00 12200.00 0.00 0.03818 24000.00 12200.00 0.00 0.03850 24200.00 12200.00 0.00 0.03861 23800.00 12400.00 0.00 0.03870 24000.00 12400.00 0.00 0.03901 24200.00 12400.00 0.00 0.03910 23800.00 12600.00 0.00 0.03924 24000.00 12600.00 0.00 0.03956 24200.00 12600.00 0.00 0.03965 23800.00 12800.00 0.00 0.03981 24000.00 12800.00 0.00 0.04014 24200.00 12800.00 0.00 0.04020 23800.00 13000.00 0.00 0.04035 24000.00 13000.00 0.00 0.04064 24200.00 13000.00 0.00 0.04066 23800.00 13200.00 0.00 0.04103 24000.00 13200.00 0.00 0.04128 24200.00 13200.00 0.00 0.04130 23800.00 13400.00 0.00 0.04189 24000.00 13400.00 0.00 0.04213 24200.00 13400.00 0.00 0.04216 23800.00 13600.00 0.00 0.04279 24000.00 13600.00 0.00 0.04304 24200.00 13600.00 0.00 0.04307 23800.00 13800.00 0.00 0.04359 24000.00 13800.00 0.00 0.04386 24200.00 13800.00 0.00 0.04387 23800.00 14000.00 0.00 0.04442 24000.00 14000.00 0.00 0.04470 23600.00 14200.00 0.00 0.04472 23800.00 14200.00 0.00 0.04538 24000.00 14200.00 0.00 0.04568 23800.00 14400.00 0.00 0.04627 24000.00 14400.00 0.00 0.04654 23800.00 14600.00 0.00 0.04713 24000.00 14600.00 0.00 0.04733 23600.00 14800.00 0.00 0.04735 23800.00 14800.00 0.00 0.04797 24000.00 14800.00 0.00 0.04810 23600.00 15000.00 0.00 0.04828 23800.00 15000.00 0.00 0.04882 24000.00 15000.00 0.00 0.04888 23600.00 15200.00 0.00 0.04908 23800.00 15200.00 0.00 0.04955 24000.00 15200.00 0.00 0.04962 23600.00 15400.00 0.00 0.04982 23800.00 15400.00 0.00 0.05030 24000.00 15400.00 0.00 0.05040 23600.00 15600.00 0.00 0.05068 23800.00 15600.00 0.00 0.05117 24000.00 15600.00 0.00 0.05128 23600.00 15800.00 0.00 0.05152 23800.00 15800.00 0.00 0.05203 24000.00 15800.00 0.00 0.05218 23600.00 16000.00 0.00 0.05264 23800.00 16000.00 0.00 0.05320 24000.00 16000.00 0.00 0.05342 23600.00 16200.00 0.00 0.05387 23800.00 16200.00 0.00 0.05447 24000.00 16200.00 0.00 0.05471 23600.00 16400.00 0.00 0.05519 23800.00 16400.00 0.00 0.05581 24000.00 16400.00 0.00 0.05604 23600.00 16600.00 0.00 0.05656 23800.00 16600.00 0.00 0.05718 24000.00 16600.00 0.00 0.05735 23600.00 16800.00 0.00 0.05773 23800.00 16800.00 0.00 0.05833 24000.00 16800.00 0.00 0.05842 23600.00 17000.00 0.00 0.05890 23800.00 17000.00 0.00 0.05948 24000.00 17000.00 0.00 0.05952 23600.00 17200.00 0.00 0.06020 23800.00 17200.00 0.00 0.06076 23600.00 17400.00 0.00 0.06158 23800.00 17400.00 0.00 0.06210 23600.00 17600.00 0.00 0.06295 23800.00 17600.00 0.00 0.06345 23600.00 17800.00 0.00 0.06446 23800.00 17800.00 0.00 0.06496 23600.00 18000.00 0.00 0.06597 23800.00 18000.00 0.00 0.06651 23600.00 18200.00 0.00 0.06742 23800.00 18200.00 0.00 0.06794 23600.00 18400.00 0.00 0.06880 23800.00 18400.00 0.00 0.06927 23600.00 18600.00 0.00 0.07025 23800.00 18600.00 0.00 0.07067 23600.00 18800.00 0.00 0.07176 23800.00 18800.00 0.00 0.07215 23600.00 19000.00 0.00 0.07314 23800.00 19000.00 0.00 0.07351 23600.00 19200.00 0.00 0.07463 23800.00 19200.00 0.00 0.07499 23600.00 19400.00 0.00 0.07626 23800.00 19400.00 0.00 0.07657 23400.00 19600.00 0.00 0.07675 23600.00 19600.00 0.00 0.07796 23800.00 19600.00 0.00 0.07818 23400.00 19800.00 0.00 0.07833 23600.00 19800.00 0.00 0.07948 23800.00 19800.00 0.00 0.07964 23400.00 20000.00 0.00 0.07986 23600.00 20000.00 0.00 0.08099 23800.00 20000.00 0.00 0.08117 23400.00 20200.00 0.00 0.08150 23600.00 20200.00 0.00 0.08264 23800.00 20200.00 0.00 0.08280 23400.00 20400.00 0.00 0.08313 23600.00 20400.00 0.00 0.08425 23800.00 20400.00 0.00 0.08437 23400.00 20600.00 0.00 0.08488 23600.00 20600.00 0.00 0.08598 23800.00 20600.00 0.00 0.08606 23400.00 20800.00 0.00 0.08659 23600.00 20800.00 0.00 0.08768 23800.00 20800.00 0.00 0.08773 23400.00 21000.00 0.00 0.08843 23600.00 21000.00 0.00 0.08952 23800.00 21000.00 0.00 0.08954 23400.00 21200.00 0.00 0.09019 23600.00 21200.00 0.00 0.09128 23400.00 21400.00 0.00 0.09200 23600.00 21400.00 0.00 0.09306 23400.00 21600.00 0.00 0.09390 23600.00 21600.00 0.00 0.09490 23400.00 21800.00 0.00 0.09570 23600.00 21800.00 0.00 0.09666 23400.00 22000.00 0.00 0.09771 23600.00 22000.00 0.00 0.09862 23400.00 22200.00 0.00 0.09974 23600.00 22200.00 0.00 0.10056 23400.00 22400.00 0.00 0.10178 23600.00 22400.00 0.00 0.10246 23400.00 22600.00 0.00 0.10363 23600.00 22600.00 0.00 0.10415 23400.00 22800.00 0.00 0.10545 23600.00 22800.00 0.00 0.10575 23400.00 23000.00 0.00 0.10689 23600.00 23000.00 0.00 0.10691 23400.00 23200.00 0.00 0.10819 23400.00 23400.00 0.00 0.10929 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23370.26 23458.07 38.18 0.10940 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/561.ctf NX, NY, NZ: 512 512 1 MODE : real