CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/558.mrc Output diagnostic file name Micrographs_0601/558.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/558.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2260.000 8108.000 Mean, RMS : 4518.249 214.0295 TITLE 1: emi2mrc: 558.emi Mon Jan 06 18:48:41 2014 TITLE 2: kV 300, Mag 78000x, Def 22480, Spot 4, Int 55.04%, Obj 88.08%, Dif 73.67% TITLE 3: X 0.335, Y 275.692, Z -41.066, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00478 10200.00 10000.00 0.00 0.00519 10400.00 10000.00 0.00 0.00568 10600.00 10000.00 0.00 0.00638 10800.00 10000.00 0.00 0.00724 11000.00 10000.00 0.00 0.00827 11200.00 10000.00 0.00 0.00948 11400.00 10000.00 0.00 0.01066 11600.00 10000.00 0.00 0.01187 11800.00 10000.00 0.00 0.01314 12000.00 10000.00 0.00 0.01454 12200.00 10000.00 0.00 0.01601 12400.00 10000.00 0.00 0.01743 12600.00 10000.00 0.00 0.01894 12800.00 10000.00 0.00 0.02049 13000.00 10000.00 0.00 0.02224 13200.00 10000.00 0.00 0.02410 13400.00 10000.00 0.00 0.02585 13600.00 10000.00 0.00 0.02766 13800.00 10000.00 0.00 0.02949 14000.00 10000.00 0.00 0.03127 14200.00 10000.00 0.00 0.03330 14400.00 10000.00 0.00 0.03566 14600.00 10000.00 0.00 0.03808 14800.00 10000.00 0.00 0.04042 15000.00 10000.00 0.00 0.04275 15200.00 10000.00 0.00 0.04516 15400.00 10000.00 0.00 0.04766 15600.00 10000.00 0.00 0.05033 15800.00 10000.00 0.00 0.05308 16000.00 10000.00 0.00 0.05604 16200.00 10000.00 0.00 0.05911 16400.00 10000.00 0.00 0.06220 16600.00 10000.00 0.00 0.06504 16800.00 10000.00 0.00 0.06689 17000.00 10000.00 0.00 0.06772 17200.00 10000.00 0.00 0.06782 16800.00 10200.00 0.00 0.06827 17000.00 10200.00 0.00 0.06902 17200.00 10200.00 0.00 0.06912 16800.00 10400.00 0.00 0.06962 17000.00 10400.00 0.00 0.07033 17200.00 10400.00 0.00 0.07043 16800.00 10600.00 0.00 0.07085 17000.00 10600.00 0.00 0.07152 17200.00 10600.00 0.00 0.07159 16800.00 10800.00 0.00 0.07221 17000.00 10800.00 0.00 0.07287 17200.00 10800.00 0.00 0.07295 16800.00 11000.00 0.00 0.07391 17000.00 11000.00 0.00 0.07459 17200.00 11000.00 0.00 0.07464 16800.00 11200.00 0.00 0.07551 17000.00 11200.00 0.00 0.07616 16800.00 11400.00 0.00 0.07722 17000.00 11400.00 0.00 0.07784 16800.00 11600.00 0.00 0.07889 17000.00 11600.00 0.00 0.07946 16800.00 11800.00 0.00 0.08066 17000.00 11800.00 0.00 0.08116 16800.00 12000.00 0.00 0.08243 17000.00 12000.00 0.00 0.08289 16800.00 12200.00 0.00 0.08417 17000.00 12200.00 0.00 0.08462 16600.00 12400.00 0.00 0.08474 16800.00 12400.00 0.00 0.08624 17000.00 12400.00 0.00 0.08663 16600.00 12600.00 0.00 0.08690 16800.00 12600.00 0.00 0.08830 17000.00 12600.00 0.00 0.08861 16600.00 12800.00 0.00 0.08908 16800.00 12800.00 0.00 0.09039 17000.00 12800.00 0.00 0.09064 16600.00 13000.00 0.00 0.09119 16800.00 13000.00 0.00 0.09244 17000.00 13000.00 0.00 0.09269 16600.00 13200.00 0.00 0.09345 16800.00 13200.00 0.00 0.09469 17000.00 13200.00 0.00 0.09488 16600.00 13400.00 0.00 0.09580 16800.00 13400.00 0.00 0.09699 17000.00 13400.00 0.00 0.09712 16600.00 13600.00 0.00 0.09826 16800.00 13600.00 0.00 0.09943 17000.00 13600.00 0.00 0.09951 16600.00 13800.00 0.00 0.10083 16800.00 13800.00 0.00 0.10197 17000.00 13800.00 0.00 0.10202 16600.00 14000.00 0.00 0.10331 16800.00 14000.00 0.00 0.10444 16600.00 14200.00 0.00 0.10598 16800.00 14200.00 0.00 0.10711 16600.00 14400.00 0.00 0.10867 16800.00 14400.00 0.00 0.10975 16600.00 14600.00 0.00 0.11149 16800.00 14600.00 0.00 0.11242 16600.00 14800.00 0.00 0.11403 16800.00 14800.00 0.00 0.11487 16600.00 15000.00 0.00 0.11677 16800.00 15000.00 0.00 0.11757 16600.00 15200.00 0.00 0.11972 16800.00 15200.00 0.00 0.12035 16600.00 15400.00 0.00 0.12272 16800.00 15400.00 0.00 0.12304 16400.00 15600.00 0.00 0.12332 16600.00 15600.00 0.00 0.12590 16400.00 15800.00 0.00 0.12628 16600.00 15800.00 0.00 0.12861 16400.00 16000.00 0.00 0.12928 16600.00 16000.00 0.00 0.13127 16400.00 16200.00 0.00 0.13164 16600.00 16200.00 0.00 0.13330 16400.00 16400.00 0.00 0.13405 16600.00 16400.00 0.00 0.13522 16400.00 16600.00 0.00 0.13587 16600.00 16600.00 0.00 0.13605 16400.00 16800.00 25.00 0.13605 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16497.62 16635.42 25.00 0.13664 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/558.ctf NX, NY, NZ: 512 512 1 MODE : real