CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/556.mrc Output diagnostic file name Micrographs_0601/556.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/556.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1932.000 7492.000 Mean, RMS : 4363.615 210.0731 TITLE 1: emi2mrc: 556.emi Mon Jan 06 18:47:04 2014 TITLE 2: kV 300, Mag 78000x, Def 22480, Spot 4, Int 55.04%, Obj 88.08%, Dif 73.67% TITLE 3: X -4.805, Y 281.896, Z -41.066, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00097 10200.00 10000.00 0.00 -0.00070 10400.00 10000.00 0.00 -0.00062 10600.00 10000.00 0.00 -0.00060 10800.00 10000.00 0.00 -0.00058 11000.00 10000.00 0.00 -0.00036 11200.00 10000.00 0.00 0.00002 11400.00 10000.00 0.00 0.00038 11600.00 10000.00 0.00 0.00092 11800.00 10000.00 0.00 0.00146 12000.00 10000.00 0.00 0.00202 12200.00 10000.00 0.00 0.00268 12400.00 10000.00 0.00 0.00334 12600.00 10000.00 0.00 0.00417 12800.00 10000.00 0.00 0.00509 13000.00 10000.00 0.00 0.00607 13200.00 10000.00 0.00 0.00710 13400.00 10000.00 0.00 0.00806 13600.00 10000.00 0.00 0.00904 13800.00 10000.00 0.00 0.01003 14000.00 10000.00 0.00 0.01117 14200.00 10000.00 0.00 0.01257 14400.00 10000.00 0.00 0.01388 14600.00 10000.00 0.00 0.01517 14800.00 10000.00 0.00 0.01677 15000.00 10000.00 0.00 0.01856 15200.00 10000.00 0.00 0.02053 15400.00 10000.00 0.00 0.02230 15600.00 10000.00 0.00 0.02384 15800.00 10000.00 0.00 0.02556 16000.00 10000.00 0.00 0.02721 16200.00 10000.00 0.00 0.02861 16400.00 10000.00 0.00 0.03000 16600.00 10000.00 0.00 0.03167 16800.00 10000.00 0.00 0.03346 17000.00 10000.00 0.00 0.03517 17200.00 10000.00 0.00 0.03683 17400.00 10000.00 0.00 0.03847 17600.00 10000.00 0.00 0.04020 17800.00 10000.00 0.00 0.04206 18000.00 10000.00 0.00 0.04400 18200.00 10000.00 0.00 0.04589 18400.00 10000.00 0.00 0.04779 18600.00 10000.00 0.00 0.04995 18800.00 10000.00 0.00 0.05180 19000.00 10000.00 0.00 0.05297 19200.00 10000.00 0.00 0.05389 19400.00 10000.00 0.00 0.05446 19600.00 10000.00 0.00 0.05466 19800.00 10000.00 0.00 0.05470 20200.00 10000.00 0.00 0.05477 19400.00 10200.00 0.00 0.05518 19600.00 10200.00 0.00 0.05538 19800.00 10200.00 0.00 0.05543 20000.00 10200.00 0.00 0.05544 20200.00 10200.00 0.00 0.05553 19400.00 10400.00 0.00 0.05592 19600.00 10400.00 0.00 0.05609 19800.00 10400.00 0.00 0.05613 20000.00 10400.00 0.00 0.05615 20200.00 10400.00 0.00 0.05624 19200.00 10600.00 0.00 0.05630 19400.00 10600.00 0.00 0.05676 19600.00 10600.00 0.00 0.05692 19800.00 10600.00 0.00 0.05700 20000.00 10600.00 0.00 0.05707 20200.00 10600.00 0.00 0.05717 19200.00 10800.00 0.00 0.05729 19400.00 10800.00 0.00 0.05773 19600.00 10800.00 0.00 0.05794 19800.00 10800.00 0.00 0.05807 20000.00 10800.00 0.00 0.05815 20200.00 10800.00 0.00 0.05822 19200.00 11000.00 0.00 0.05846 19400.00 11000.00 0.00 0.05890 19600.00 11000.00 0.00 0.05916 19800.00 11000.00 0.00 0.05927 20000.00 11000.00 0.00 0.05932 20200.00 11000.00 0.00 0.05932 19200.00 11200.00 0.00 0.05960 19400.00 11200.00 0.00 0.06004 19600.00 11200.00 0.00 0.06032 19800.00 11200.00 0.00 0.06038 19200.00 11400.00 0.00 0.06085 19400.00 11400.00 0.00 0.06130 19600.00 11400.00 0.00 0.06154 19200.00 11600.00 0.00 0.06207 19400.00 11600.00 0.00 0.06251 19600.00 11600.00 0.00 0.06269 19200.00 11800.00 0.00 0.06329 19400.00 11800.00 0.00 0.06368 19600.00 11800.00 0.00 0.06379 19000.00 12000.00 0.00 0.06392 19200.00 12000.00 0.00 0.06463 19400.00 12000.00 0.00 0.06498 19600.00 12000.00 0.00 0.06506 19000.00 12200.00 0.00 0.06544 19200.00 12200.00 0.00 0.06608 19400.00 12200.00 0.00 0.06641 19600.00 12200.00 0.00 0.06649 19000.00 12400.00 0.00 0.06696 19200.00 12400.00 0.00 0.06754 19400.00 12400.00 0.00 0.06786 19600.00 12400.00 0.00 0.06790 19000.00 12600.00 0.00 0.06819 19200.00 12600.00 0.00 0.06875 19400.00 12600.00 0.00 0.06907 19600.00 12600.00 0.00 0.06910 19000.00 12800.00 0.00 0.06947 19200.00 12800.00 0.00 0.07007 19400.00 12800.00 0.00 0.07045 19600.00 12800.00 0.00 0.07052 19800.00 12800.00 0.00 0.07052 20000.00 12800.00 0.00 0.07058 19000.00 13000.00 0.00 0.07092 19200.00 13000.00 0.00 0.07161 19400.00 13000.00 0.00 0.07203 19600.00 13000.00 0.00 0.07212 19800.00 13000.00 0.00 0.07215 20000.00 13000.00 0.00 0.07218 19000.00 13200.00 0.00 0.07247 19200.00 13200.00 0.00 0.07321 19400.00 13200.00 0.00 0.07363 19600.00 13200.00 0.00 0.07369 19800.00 13200.00 0.00 0.07371 19000.00 13400.00 0.00 0.07405 19200.00 13400.00 0.00 0.07478 19400.00 13400.00 0.00 0.07515 19600.00 13400.00 0.00 0.07518 19800.00 13400.00 0.00 0.07519 19000.00 13600.00 0.00 0.07567 19200.00 13600.00 0.00 0.07637 19400.00 13600.00 0.00 0.07672 19600.00 13600.00 0.00 0.07676 19800.00 13600.00 0.00 0.07678 19000.00 13800.00 0.00 0.07739 19200.00 13800.00 0.00 0.07810 19400.00 13800.00 0.00 0.07844 19600.00 13800.00 0.00 0.07847 19000.00 14000.00 0.00 0.07904 19200.00 14000.00 0.00 0.07970 19400.00 14000.00 0.00 0.07998 19000.00 14200.00 0.00 0.08053 19200.00 14200.00 0.00 0.08113 19400.00 14200.00 0.00 0.08136 19000.00 14400.00 0.00 0.08201 19200.00 14400.00 0.00 0.08258 19400.00 14400.00 0.00 0.08282 19000.00 14600.00 0.00 0.08360 19200.00 14600.00 0.00 0.08417 19400.00 14600.00 0.00 0.08438 19000.00 14800.00 0.00 0.08520 19200.00 14800.00 0.00 0.08574 19400.00 14800.00 0.00 0.08592 19000.00 15000.00 0.00 0.08709 19200.00 15000.00 0.00 0.08761 19400.00 15000.00 0.00 0.08777 19000.00 15200.00 0.00 0.08889 19200.00 15200.00 0.00 0.08942 19400.00 15200.00 0.00 0.08958 19000.00 15400.00 0.00 0.09068 19200.00 15400.00 0.00 0.09124 19400.00 15400.00 0.00 0.09137 18800.00 15600.00 0.00 0.09142 19000.00 15600.00 0.00 0.09267 19200.00 15600.00 0.00 0.09316 19400.00 15600.00 0.00 0.09319 19000.00 15800.00 0.00 0.09435 19200.00 15800.00 0.00 0.09477 18800.00 16000.00 0.00 0.09501 19000.00 16000.00 0.00 0.09620 19200.00 16000.00 0.00 0.09661 18800.00 16200.00 0.00 0.09689 19000.00 16200.00 0.00 0.09813 19200.00 16200.00 0.00 0.09852 18800.00 16400.00 0.00 0.09898 19000.00 16400.00 0.00 0.10024 19200.00 16400.00 0.00 0.10054 18800.00 16600.00 0.00 0.10123 19000.00 16600.00 0.00 0.10246 19200.00 16600.00 0.00 0.10266 18800.00 16800.00 0.00 0.10345 19000.00 16800.00 0.00 0.10465 19200.00 16800.00 0.00 0.10475 18800.00 17000.00 0.00 0.10575 19000.00 17000.00 0.00 0.10690 18800.00 17200.00 0.00 0.10805 19000.00 17200.00 0.00 0.10911 18800.00 17400.00 0.00 0.11048 19000.00 17400.00 0.00 0.11138 18800.00 17600.00 0.00 0.11278 19000.00 17600.00 0.00 0.11345 18800.00 17800.00 0.00 0.11525 19000.00 17800.00 0.00 0.11568 18600.00 18000.00 0.00 0.11570 18800.00 18000.00 0.00 0.11776 19000.00 18000.00 0.00 0.11793 18600.00 18200.00 0.00 0.11834 18800.00 18200.00 0.00 0.12018 18600.00 18400.00 0.00 0.12050 18800.00 18400.00 0.00 0.12216 18600.00 18600.00 0.00 0.12234 18800.00 18600.00 0.00 0.12386 18600.00 18800.00 0.00 0.12407 18800.00 18800.00 0.00 0.12506 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18769.29 18834.18 16.00 0.12511 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/556.ctf NX, NY, NZ: 512 512 1 MODE : real