CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/555.mrc Output diagnostic file name Micrographs_0601/555.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/555.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2424.000 8462.000 Mean, RMS : 4599.246 224.2351 TITLE 1: emi2mrc: 555.emi Mon Jan 06 18:34:31 2014 TITLE 2: kV 300, Mag 78000x, Def 21170, Spot 4, Int 55.04%, Obj 88.03%, Dif 73.67% TITLE 3: X 4.668, Y 282.674, Z -39.500, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01998 11400.00 10000.00 0.00 -0.01987 11600.00 10000.00 0.00 -0.01961 11800.00 10000.00 0.00 -0.01930 12000.00 10000.00 0.00 -0.01915 12200.00 10000.00 0.00 -0.01889 12400.00 10000.00 0.00 -0.01861 12600.00 10000.00 0.00 -0.01831 12800.00 10000.00 0.00 -0.01804 13000.00 10000.00 0.00 -0.01784 13200.00 10000.00 0.00 -0.01760 13400.00 10000.00 0.00 -0.01739 13600.00 10000.00 0.00 -0.01717 13800.00 10000.00 0.00 -0.01697 14000.00 10000.00 0.00 -0.01655 14200.00 10000.00 0.00 -0.01610 14400.00 10000.00 0.00 -0.01575 14600.00 10000.00 0.00 -0.01534 14800.00 10000.00 0.00 -0.01505 15000.00 10000.00 0.00 -0.01473 15200.00 10000.00 0.00 -0.01427 15400.00 10000.00 0.00 -0.01373 15600.00 10000.00 0.00 -0.01310 15800.00 10000.00 0.00 -0.01243 16000.00 10000.00 0.00 -0.01170 16200.00 10000.00 0.00 -0.01091 16400.00 10000.00 0.00 -0.01006 16600.00 10000.00 0.00 -0.00917 16800.00 10000.00 0.00 -0.00822 17000.00 10000.00 0.00 -0.00726 17200.00 10000.00 0.00 -0.00634 17400.00 10000.00 0.00 -0.00549 17600.00 10000.00 0.00 -0.00460 17800.00 10000.00 0.00 -0.00370 18000.00 10000.00 0.00 -0.00285 18200.00 10000.00 0.00 -0.00180 18400.00 10000.00 0.00 -0.00072 18600.00 10000.00 0.00 0.00031 18800.00 10000.00 0.00 0.00140 19000.00 10000.00 0.00 0.00233 19200.00 10000.00 0.00 0.00334 19400.00 10000.00 0.00 0.00440 19600.00 10000.00 0.00 0.00546 19800.00 10000.00 0.00 0.00666 20000.00 10000.00 0.00 0.00794 20200.00 10000.00 0.00 0.00932 20400.00 10000.00 0.00 0.01065 20600.00 10000.00 0.00 0.01199 20800.00 10000.00 0.00 0.01342 21000.00 10000.00 0.00 0.01486 21200.00 10000.00 0.00 0.01637 21400.00 10000.00 0.00 0.01787 21600.00 10000.00 0.00 0.01947 21800.00 10000.00 0.00 0.02119 22000.00 10000.00 0.00 0.02296 22200.00 10000.00 0.00 0.02481 22400.00 10000.00 0.00 0.02666 22600.00 10000.00 0.00 0.02855 22800.00 10000.00 0.00 0.03049 23000.00 10000.00 0.00 0.03206 23200.00 10000.00 0.00 0.03294 23400.00 10000.00 0.00 0.03338 23600.00 10000.00 0.00 0.03367 23800.00 10000.00 0.00 0.03388 24400.00 10000.00 0.00 0.03393 23600.00 10200.00 0.00 0.03413 23800.00 10200.00 0.00 0.03433 23600.00 10400.00 0.00 0.03463 23800.00 10400.00 0.00 0.03479 23400.00 10600.00 0.00 0.03480 23600.00 10600.00 0.00 0.03513 23800.00 10600.00 0.00 0.03525 23600.00 10800.00 0.00 0.03552 23800.00 10800.00 0.00 0.03563 23400.00 11000.00 0.00 0.03565 23600.00 11000.00 0.00 0.03596 23800.00 11000.00 0.00 0.03609 23400.00 11200.00 0.00 0.03618 23600.00 11200.00 0.00 0.03651 23800.00 11200.00 0.00 0.03664 23400.00 11400.00 0.00 0.03668 23600.00 11400.00 0.00 0.03702 23800.00 11400.00 0.00 0.03715 23400.00 11600.00 0.00 0.03718 23600.00 11600.00 0.00 0.03754 23800.00 11600.00 0.00 0.03766 23400.00 11800.00 0.00 0.03785 23600.00 11800.00 0.00 0.03818 23800.00 11800.00 0.00 0.03829 23400.00 12000.00 0.00 0.03852 23600.00 12000.00 0.00 0.03883 23800.00 12000.00 0.00 0.03895 23400.00 12200.00 0.00 0.03921 23600.00 12200.00 0.00 0.03953 23800.00 12200.00 0.00 0.03966 23400.00 12400.00 0.00 0.03993 23600.00 12400.00 0.00 0.04025 23800.00 12400.00 0.00 0.04037 23400.00 12600.00 0.00 0.04059 23600.00 12600.00 0.00 0.04093 23800.00 12600.00 0.00 0.04102 23400.00 12800.00 0.00 0.04141 23600.00 12800.00 0.00 0.04175 23800.00 12800.00 0.00 0.04180 23400.00 13000.00 0.00 0.04226 23600.00 13000.00 0.00 0.04256 23800.00 13000.00 0.00 0.04258 23400.00 13200.00 0.00 0.04307 23600.00 13200.00 0.00 0.04336 23800.00 13200.00 0.00 0.04338 23400.00 13400.00 0.00 0.04397 23600.00 13400.00 0.00 0.04429 23800.00 13400.00 0.00 0.04433 23400.00 13600.00 0.00 0.04498 23600.00 13600.00 0.00 0.04532 23800.00 13600.00 0.00 0.04533 23200.00 13800.00 0.00 0.04534 23400.00 13800.00 0.00 0.04604 23600.00 13800.00 0.00 0.04634 23200.00 14000.00 0.00 0.04641 23400.00 14000.00 0.00 0.04708 23600.00 14000.00 0.00 0.04730 23200.00 14200.00 0.00 0.04745 23400.00 14200.00 0.00 0.04807 23600.00 14200.00 0.00 0.04821 23200.00 14400.00 0.00 0.04845 23400.00 14400.00 0.00 0.04899 23600.00 14400.00 0.00 0.04908 23200.00 14600.00 0.00 0.04944 23400.00 14600.00 0.00 0.04990 23600.00 14600.00 0.00 0.04996 23200.00 14800.00 0.00 0.05041 23400.00 14800.00 0.00 0.05085 23600.00 14800.00 0.00 0.05094 23200.00 15000.00 0.00 0.05142 23400.00 15000.00 0.00 0.05187 23600.00 15000.00 0.00 0.05199 23200.00 15200.00 0.00 0.05239 23400.00 15200.00 0.00 0.05283 23600.00 15200.00 0.00 0.05292 23200.00 15400.00 0.00 0.05342 23400.00 15400.00 0.00 0.05384 23600.00 15400.00 0.00 0.05391 23200.00 15600.00 0.00 0.05450 23400.00 15600.00 0.00 0.05488 23600.00 15600.00 0.00 0.05493 23200.00 15800.00 0.00 0.05544 23400.00 15800.00 0.00 0.05581 23600.00 15800.00 0.00 0.05585 23200.00 16000.00 0.00 0.05651 23400.00 16000.00 0.00 0.05689 23600.00 16000.00 0.00 0.05692 23200.00 16200.00 0.00 0.05774 23400.00 16200.00 0.00 0.05811 23600.00 16200.00 0.00 0.05812 23200.00 16400.00 0.00 0.05901 23400.00 16400.00 0.00 0.05936 23000.00 16600.00 0.00 0.05939 23200.00 16600.00 0.00 0.06027 23400.00 16600.00 0.00 0.06061 23600.00 16600.00 0.00 0.06064 23000.00 16800.00 0.00 0.06076 23200.00 16800.00 0.00 0.06163 23400.00 16800.00 0.00 0.06198 23600.00 16800.00 0.00 0.06201 23000.00 17000.00 0.00 0.06212 23200.00 17000.00 0.00 0.06299 23400.00 17000.00 0.00 0.06335 23600.00 17000.00 0.00 0.06336 23000.00 17200.00 0.00 0.06342 23200.00 17200.00 0.00 0.06431 23400.00 17200.00 0.00 0.06465 23000.00 17400.00 0.00 0.06480 23200.00 17400.00 0.00 0.06569 23400.00 17400.00 0.00 0.06602 23000.00 17600.00 0.00 0.06615 23200.00 17600.00 0.00 0.06704 23400.00 17600.00 0.00 0.06735 23000.00 17800.00 0.00 0.06762 23200.00 17800.00 0.00 0.06848 23400.00 17800.00 0.00 0.06881 23000.00 18000.00 0.00 0.06901 23200.00 18000.00 0.00 0.06988 23400.00 18000.00 0.00 0.07023 23000.00 18200.00 0.00 0.07037 23200.00 18200.00 0.00 0.07128 23400.00 18200.00 0.00 0.07165 23000.00 18400.00 0.00 0.07181 23200.00 18400.00 0.00 0.07275 23400.00 18400.00 0.00 0.07310 23000.00 18600.00 0.00 0.07327 23200.00 18600.00 0.00 0.07423 23400.00 18600.00 0.00 0.07456 23000.00 18800.00 0.00 0.07482 23200.00 18800.00 0.00 0.07579 23400.00 18800.00 0.00 0.07611 23000.00 19000.00 0.00 0.07640 23200.00 19000.00 0.00 0.07735 23400.00 19000.00 0.00 0.07764 23000.00 19200.00 0.00 0.07808 23200.00 19200.00 0.00 0.07902 23400.00 19200.00 0.00 0.07927 23000.00 19400.00 0.00 0.07967 23200.00 19400.00 0.00 0.08056 23400.00 19400.00 0.00 0.08075 23000.00 19600.00 0.00 0.08119 23200.00 19600.00 0.00 0.08204 23400.00 19600.00 0.00 0.08215 23000.00 19800.00 0.00 0.08287 23200.00 19800.00 0.00 0.08370 23400.00 19800.00 0.00 0.08378 23000.00 20000.00 0.00 0.08471 23200.00 20000.00 0.00 0.08553 23400.00 20000.00 0.00 0.08557 23000.00 20200.00 0.00 0.08651 23200.00 20200.00 0.00 0.08731 23000.00 20400.00 0.00 0.08833 23200.00 20400.00 0.00 0.08910 23000.00 20600.00 0.00 0.09036 23200.00 20600.00 0.00 0.09105 23000.00 20800.00 0.00 0.09231 23200.00 20800.00 0.00 0.09289 23000.00 21000.00 0.00 0.09436 23200.00 21000.00 0.00 0.09488 23000.00 21200.00 0.00 0.09662 23200.00 21200.00 0.00 0.09707 23000.00 21400.00 0.00 0.09905 23200.00 21400.00 0.00 0.09937 23000.00 21600.00 0.00 0.10123 23200.00 21600.00 0.00 0.10139 22800.00 21800.00 0.00 0.10145 23000.00 21800.00 0.00 0.10329 23200.00 21800.00 0.00 0.10330 22800.00 22000.00 0.00 0.10381 23000.00 22000.00 0.00 0.10553 22800.00 22200.00 0.00 0.10592 23000.00 22200.00 0.00 0.10750 22800.00 22400.00 0.00 0.10817 23000.00 22400.00 0.00 0.10956 22800.00 22600.00 0.00 0.11021 23000.00 22600.00 0.00 0.11132 22800.00 22800.00 0.00 0.11209 23000.00 22800.00 0.00 0.11273 22800.00 23000.00 0.00 0.11293 22800.00 23000.00 5.00 0.11304 22800.00 23000.00 10.00 0.11313 22800.00 23000.00 15.00 0.11322 22800.00 23000.00 20.00 0.11329 22800.00 23000.00 25.00 0.11335 22800.00 23000.00 30.00 0.11339 22800.00 23000.00 35.00 0.11341 22800.00 23000.00 40.00 0.11341 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22848.55 22993.00 34.73 0.11348 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/555.ctf NX, NY, NZ: 512 512 1 MODE : real