CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/549.mrc Output diagnostic file name Micrographs_0601/549.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/549.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2286.000 8798.000 Mean, RMS : 4569.930 214.6503 TITLE 1: emi2mrc: 549.emi Mon Jan 06 18:40:23 2014 TITLE 2: kV 300, Mag 78000x, Def 21490, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X 6.048, Y 276.133, Z -39.500, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01032 10200.00 10000.00 0.00 0.01156 10400.00 10000.00 0.00 0.01270 10600.00 10000.00 0.00 0.01411 10800.00 10000.00 0.00 0.01535 11000.00 10000.00 0.00 0.01658 11200.00 10000.00 0.00 0.01816 11400.00 10000.00 0.00 0.01990 11600.00 10000.00 0.00 0.02182 11800.00 10000.00 0.00 0.02377 12000.00 10000.00 0.00 0.02581 12200.00 10000.00 0.00 0.02788 12400.00 10000.00 0.00 0.03011 12600.00 10000.00 0.00 0.03231 12800.00 10000.00 0.00 0.03448 13000.00 10000.00 0.00 0.03687 13200.00 10000.00 0.00 0.03934 13400.00 10000.00 0.00 0.04193 13600.00 10000.00 0.00 0.04439 13800.00 10000.00 0.00 0.04683 14000.00 10000.00 0.00 0.04943 14200.00 10000.00 0.00 0.05216 14400.00 10000.00 0.00 0.05505 14600.00 10000.00 0.00 0.05799 14800.00 10000.00 0.00 0.06104 15000.00 10000.00 0.00 0.06405 15200.00 10000.00 0.00 0.06702 15400.00 10000.00 0.00 0.06988 15600.00 10000.00 0.00 0.07200 15800.00 10000.00 0.00 0.07295 16000.00 10000.00 0.00 0.07314 15600.00 10200.00 0.00 0.07352 15800.00 10200.00 0.00 0.07441 16000.00 10200.00 0.00 0.07456 15600.00 10400.00 0.00 0.07520 15800.00 10400.00 0.00 0.07604 16000.00 10400.00 0.00 0.07616 15600.00 10600.00 0.00 0.07692 15800.00 10600.00 0.00 0.07771 16000.00 10600.00 0.00 0.07780 15600.00 10800.00 0.00 0.07863 15800.00 10800.00 0.00 0.07938 16000.00 10800.00 0.00 0.07943 15600.00 11000.00 0.00 0.08040 15800.00 11000.00 0.00 0.08112 15600.00 11200.00 0.00 0.08199 15800.00 11200.00 0.00 0.08269 15600.00 11400.00 0.00 0.08398 15800.00 11400.00 0.00 0.08470 16000.00 11400.00 0.00 0.08472 15600.00 11600.00 0.00 0.08611 15800.00 11600.00 0.00 0.08681 15600.00 11800.00 0.00 0.08833 15800.00 11800.00 0.00 0.08898 15600.00 12000.00 0.00 0.09058 15800.00 12000.00 0.00 0.09114 15600.00 12200.00 0.00 0.09277 15800.00 12200.00 0.00 0.09322 15400.00 12400.00 0.00 0.09325 15600.00 12400.00 0.00 0.09514 15800.00 12400.00 0.00 0.09550 15400.00 12600.00 0.00 0.09560 15600.00 12600.00 0.00 0.09740 15800.00 12600.00 0.00 0.09767 15400.00 12800.00 0.00 0.09791 15600.00 12800.00 0.00 0.09959 15800.00 12800.00 0.00 0.09977 15400.00 13000.00 0.00 0.09989 15600.00 13000.00 0.00 0.10146 15800.00 13000.00 0.00 0.10159 15400.00 13200.00 0.00 0.10209 15600.00 13200.00 0.00 0.10361 15800.00 13200.00 0.00 0.10370 15400.00 13400.00 0.00 0.10445 15600.00 13400.00 0.00 0.10594 15800.00 13400.00 0.00 0.10596 15400.00 13600.00 0.00 0.10683 15600.00 13600.00 0.00 0.10824 15400.00 13800.00 0.00 0.10906 15600.00 13800.00 0.00 0.11033 15400.00 14000.00 0.00 0.11093 15600.00 14000.00 0.00 0.11205 15400.00 14200.00 0.00 0.11288 15600.00 14200.00 0.00 0.11378 15400.00 14400.00 0.00 0.11453 15600.00 14400.00 0.00 0.11523 15400.00 14600.00 0.00 0.11640 15600.00 14600.00 0.00 0.11688 15400.00 14800.00 0.00 0.11812 15600.00 14800.00 0.00 0.11836 15400.00 15000.00 0.00 0.11984 15200.00 15200.00 0.00 0.11998 15400.00 15200.00 0.00 0.12143 15400.00 15400.00 0.00 0.12232 15400.00 15600.00 0.00 0.12266 15400.00 15600.00 5.00 0.12284 15400.00 15600.00 10.00 0.12299 15400.00 15600.00 15.00 0.12312 15400.00 15600.00 20.00 0.12321 15400.00 15600.00 25.00 0.12327 15400.00 15600.00 30.00 0.12330 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15313.04 15634.49 32.14 0.12352 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/549.ctf NX, NY, NZ: 512 512 1 MODE : real