CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/548.mrc Output diagnostic file name Micrographs_0601/548.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/548.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2566.000 8720.000 Mean, RMS : 4578.847 218.8114 TITLE 1: emi2mrc: 548.emi Mon Jan 06 18:39:40 2014 TITLE 2: kV 300, Mag 78000x, Def 21490, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X 4.310, Y 278.527, Z -39.500, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01783 10200.00 10000.00 0.00 0.01882 10400.00 10000.00 0.00 0.01965 10600.00 10000.00 0.00 0.02065 10800.00 10000.00 0.00 0.02160 11000.00 10000.00 0.00 0.02276 11200.00 10000.00 0.00 0.02411 11400.00 10000.00 0.00 0.02562 11600.00 10000.00 0.00 0.02734 11800.00 10000.00 0.00 0.02919 12000.00 10000.00 0.00 0.03116 12200.00 10000.00 0.00 0.03302 12400.00 10000.00 0.00 0.03500 12600.00 10000.00 0.00 0.03712 12800.00 10000.00 0.00 0.03944 13000.00 10000.00 0.00 0.04193 13200.00 10000.00 0.00 0.04443 13400.00 10000.00 0.00 0.04703 13600.00 10000.00 0.00 0.04970 13800.00 10000.00 0.00 0.05268 14000.00 10000.00 0.00 0.05581 14200.00 10000.00 0.00 0.05914 14400.00 10000.00 0.00 0.06240 14600.00 10000.00 0.00 0.06576 14800.00 10000.00 0.00 0.06945 15000.00 10000.00 0.00 0.07329 15200.00 10000.00 0.00 0.07717 15400.00 10000.00 0.00 0.07968 15600.00 10000.00 0.00 0.08059 15800.00 10000.00 0.00 0.08076 15400.00 10200.00 0.00 0.08153 15600.00 10200.00 0.00 0.08241 15800.00 10200.00 0.00 0.08248 15400.00 10400.00 0.00 0.08353 15600.00 10400.00 0.00 0.08432 15400.00 10600.00 0.00 0.08550 15600.00 10600.00 0.00 0.08621 15400.00 10800.00 0.00 0.08740 15600.00 10800.00 0.00 0.08806 15400.00 11000.00 0.00 0.08959 15600.00 11000.00 0.00 0.09022 15400.00 11200.00 0.00 0.09167 15600.00 11200.00 0.00 0.09230 15400.00 11400.00 0.00 0.09401 15600.00 11400.00 0.00 0.09465 15400.00 11600.00 0.00 0.09642 15600.00 11600.00 0.00 0.09705 15400.00 11800.00 0.00 0.09910 15600.00 11800.00 0.00 0.09972 15400.00 12000.00 0.00 0.10207 15600.00 12000.00 0.00 0.10264 15200.00 12200.00 0.00 0.10283 15400.00 12200.00 0.00 0.10523 15600.00 12200.00 0.00 0.10575 15200.00 12400.00 0.00 0.10619 15400.00 12400.00 0.00 0.10855 15600.00 12400.00 0.00 0.10899 15200.00 12600.00 0.00 0.10934 15400.00 12600.00 0.00 0.11167 15600.00 12600.00 0.00 0.11206 15200.00 12800.00 0.00 0.11281 15400.00 12800.00 0.00 0.11512 15600.00 12800.00 0.00 0.11542 15200.00 13000.00 0.00 0.11612 15400.00 13000.00 0.00 0.11838 15600.00 13000.00 0.00 0.11854 15200.00 13200.00 0.00 0.11955 15400.00 13200.00 0.00 0.12176 15200.00 13400.00 0.00 0.12297 15400.00 13400.00 0.00 0.12512 15200.00 13600.00 0.00 0.12657 15400.00 13600.00 0.00 0.12867 15200.00 13800.00 0.00 0.13062 15400.00 13800.00 0.00 0.13257 15200.00 14000.00 0.00 0.13443 15400.00 14000.00 0.00 0.13617 15200.00 14200.00 0.00 0.13855 15400.00 14200.00 0.00 0.14006 15200.00 14400.00 0.00 0.14277 15400.00 14400.00 0.00 0.14398 15200.00 14600.00 0.00 0.14743 15400.00 14600.00 0.00 0.14814 15200.00 14800.00 0.00 0.15162 15400.00 14800.00 0.00 0.15171 15200.00 15000.00 0.00 0.15601 15000.00 15200.00 0.00 0.15641 15200.00 15200.00 0.00 0.15951 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15248.00 15177.94 -65.90 0.15971 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/548.ctf NX, NY, NZ: 512 512 1 MODE : real