CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/547.mrc Output diagnostic file name Micrographs_0601/547.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/547.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2406.000 8098.000 Mean, RMS : 4578.787 218.8089 TITLE 1: emi2mrc: 547.emi Mon Jan 06 18:38:56 2014 TITLE 2: kV 300, Mag 78000x, Def 21490, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X 2.691, Y 280.651, Z -39.500, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01028 10200.00 10000.00 0.00 0.01117 10400.00 10000.00 0.00 0.01201 10600.00 10000.00 0.00 0.01297 10800.00 10000.00 0.00 0.01406 11000.00 10000.00 0.00 0.01510 11200.00 10000.00 0.00 0.01622 11400.00 10000.00 0.00 0.01746 11600.00 10000.00 0.00 0.01870 11800.00 10000.00 0.00 0.02000 12000.00 10000.00 0.00 0.02147 12200.00 10000.00 0.00 0.02294 12400.00 10000.00 0.00 0.02458 12600.00 10000.00 0.00 0.02630 12800.00 10000.00 0.00 0.02805 13000.00 10000.00 0.00 0.03006 13200.00 10000.00 0.00 0.03232 13400.00 10000.00 0.00 0.03468 13600.00 10000.00 0.00 0.03688 13800.00 10000.00 0.00 0.03921 14000.00 10000.00 0.00 0.04156 14200.00 10000.00 0.00 0.04401 14400.00 10000.00 0.00 0.04678 14600.00 10000.00 0.00 0.04946 14800.00 10000.00 0.00 0.05227 15000.00 10000.00 0.00 0.05546 15200.00 10000.00 0.00 0.05890 15400.00 10000.00 0.00 0.06233 15600.00 10000.00 0.00 0.06589 15800.00 10000.00 0.00 0.06951 16000.00 10000.00 0.00 0.07210 16200.00 10000.00 0.00 0.07303 16000.00 10200.00 0.00 0.07355 16200.00 10200.00 0.00 0.07448 16000.00 10400.00 0.00 0.07497 16200.00 10400.00 0.00 0.07588 16000.00 10600.00 0.00 0.07660 16200.00 10600.00 0.00 0.07749 16000.00 10800.00 0.00 0.07847 16200.00 10800.00 0.00 0.07934 16000.00 11000.00 0.00 0.08047 16200.00 11000.00 0.00 0.08130 16000.00 11200.00 0.00 0.08220 16200.00 11200.00 0.00 0.08303 16000.00 11400.00 0.00 0.08416 16200.00 11400.00 0.00 0.08501 16000.00 11600.00 0.00 0.08619 16200.00 11600.00 0.00 0.08706 16000.00 11800.00 0.00 0.08831 16200.00 11800.00 0.00 0.08922 16000.00 12000.00 0.00 0.09080 16200.00 12000.00 0.00 0.09174 16000.00 12200.00 0.00 0.09342 16200.00 12200.00 0.00 0.09429 16000.00 12400.00 0.00 0.09595 16200.00 12400.00 0.00 0.09673 16000.00 12600.00 0.00 0.09856 16200.00 12600.00 0.00 0.09927 16000.00 12800.00 0.00 0.10143 16200.00 12800.00 0.00 0.10200 16000.00 13000.00 0.00 0.10431 16200.00 13000.00 0.00 0.10472 16000.00 13200.00 0.00 0.10750 16200.00 13200.00 0.00 0.10771 16000.00 13400.00 0.00 0.11050 16200.00 13400.00 0.00 0.11056 15800.00 13600.00 0.00 0.11083 16000.00 13600.00 0.00 0.11352 15800.00 13800.00 0.00 0.11395 16000.00 13800.00 0.00 0.11659 15800.00 14000.00 0.00 0.11699 16000.00 14000.00 0.00 0.11961 15800.00 14200.00 0.00 0.12051 16000.00 14200.00 0.00 0.12312 15800.00 14400.00 0.00 0.12407 16000.00 14400.00 0.00 0.12662 15800.00 14600.00 0.00 0.12803 16000.00 14600.00 0.00 0.13041 15800.00 14800.00 0.00 0.13192 16000.00 14800.00 0.00 0.13414 15800.00 15000.00 0.00 0.13621 16000.00 15000.00 0.00 0.13828 15800.00 15200.00 0.00 0.14082 16000.00 15200.00 0.00 0.14268 15800.00 15400.00 0.00 0.14537 16000.00 15400.00 0.00 0.14680 15800.00 15600.00 0.00 0.15019 16000.00 15600.00 0.00 0.15078 15800.00 15800.00 0.00 0.15355 15800.00 16000.00 0.00 0.15430 15800.00 16000.00 5.00 0.15443 15800.00 16000.00 10.00 0.15454 15800.00 16000.00 15.00 0.15463 15800.00 16000.00 20.00 0.15469 15800.00 16000.00 25.00 0.15471 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15814.07 15946.46 25.44 0.15489 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/547.ctf NX, NY, NZ: 512 512 1 MODE : real