CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/545.mrc Output diagnostic file name Micrographs_0601/545.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/545.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2522.000 8998.000 Mean, RMS : 4440.139 214.4140 TITLE 1: emi2mrc: 545.emi Mon Jan 06 18:36:37 2014 TITLE 2: kV 300, Mag 78000x, Def 21490, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X 1.271, Y 287.117, Z -39.500, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00012 10200.00 10000.00 0.00 0.00028 10400.00 10000.00 0.00 0.00050 10600.00 10000.00 0.00 0.00070 10800.00 10000.00 0.00 0.00093 11000.00 10000.00 0.00 0.00131 11200.00 10000.00 0.00 0.00176 11400.00 10000.00 0.00 0.00228 11600.00 10000.00 0.00 0.00281 11800.00 10000.00 0.00 0.00323 12000.00 10000.00 0.00 0.00362 12200.00 10000.00 0.00 0.00422 12400.00 10000.00 0.00 0.00498 12600.00 10000.00 0.00 0.00580 12800.00 10000.00 0.00 0.00668 13000.00 10000.00 0.00 0.00756 13200.00 10000.00 0.00 0.00842 13400.00 10000.00 0.00 0.00924 13600.00 10000.00 0.00 0.00998 13800.00 10000.00 0.00 0.01058 14000.00 10000.00 0.00 0.01122 14200.00 10000.00 0.00 0.01206 14400.00 10000.00 0.00 0.01304 14600.00 10000.00 0.00 0.01425 14800.00 10000.00 0.00 0.01539 15000.00 10000.00 0.00 0.01646 15200.00 10000.00 0.00 0.01788 15400.00 10000.00 0.00 0.01935 15600.00 10000.00 0.00 0.02083 15800.00 10000.00 0.00 0.02245 16000.00 10000.00 0.00 0.02408 16200.00 10000.00 0.00 0.02570 16400.00 10000.00 0.00 0.02717 16600.00 10000.00 0.00 0.02863 16800.00 10000.00 0.00 0.03018 17000.00 10000.00 0.00 0.03172 17200.00 10000.00 0.00 0.03323 17400.00 10000.00 0.00 0.03487 17600.00 10000.00 0.00 0.03648 17800.00 10000.00 0.00 0.03807 18000.00 10000.00 0.00 0.03965 18200.00 10000.00 0.00 0.04119 18400.00 10000.00 0.00 0.04289 18600.00 10000.00 0.00 0.04464 18800.00 10000.00 0.00 0.04658 19000.00 10000.00 0.00 0.04848 19200.00 10000.00 0.00 0.05025 19400.00 10000.00 0.00 0.05192 19600.00 10000.00 0.00 0.05307 19800.00 10000.00 0.00 0.05384 20000.00 10000.00 0.00 0.05432 20200.00 10000.00 0.00 0.05456 20400.00 10000.00 0.00 0.05461 20000.00 10200.00 0.00 0.05491 20200.00 10200.00 0.00 0.05515 20400.00 10200.00 0.00 0.05523 20000.00 10400.00 0.00 0.05550 20200.00 10400.00 0.00 0.05577 20400.00 10400.00 0.00 0.05584 20000.00 10600.00 0.00 0.05623 20200.00 10600.00 0.00 0.05646 20400.00 10600.00 0.00 0.05647 20000.00 10800.00 0.00 0.05693 20200.00 10800.00 0.00 0.05710 19800.00 11000.00 0.00 0.05723 20000.00 11000.00 0.00 0.05771 20200.00 11000.00 0.00 0.05786 19800.00 11200.00 0.00 0.05799 20000.00 11200.00 0.00 0.05848 20200.00 11200.00 0.00 0.05864 20400.00 11200.00 0.00 0.05864 19800.00 11400.00 0.00 0.05889 20000.00 11400.00 0.00 0.05935 20200.00 11400.00 0.00 0.05948 19800.00 11600.00 0.00 0.05982 20000.00 11600.00 0.00 0.06023 20200.00 11600.00 0.00 0.06032 19800.00 11800.00 0.00 0.06083 20000.00 11800.00 0.00 0.06117 20200.00 11800.00 0.00 0.06125 19800.00 12000.00 0.00 0.06185 20000.00 12000.00 0.00 0.06214 20200.00 12000.00 0.00 0.06221 19800.00 12200.00 0.00 0.06283 20000.00 12200.00 0.00 0.06311 20200.00 12200.00 0.00 0.06322 19600.00 12400.00 0.00 0.06330 19800.00 12400.00 0.00 0.06405 20000.00 12400.00 0.00 0.06436 20200.00 12400.00 0.00 0.06451 20400.00 12400.00 0.00 0.06453 19600.00 12600.00 0.00 0.06458 19800.00 12600.00 0.00 0.06532 20000.00 12600.00 0.00 0.06562 20200.00 12600.00 0.00 0.06578 19600.00 12800.00 0.00 0.06588 19800.00 12800.00 0.00 0.06659 20000.00 12800.00 0.00 0.06689 20200.00 12800.00 0.00 0.06707 19600.00 13000.00 0.00 0.06714 19800.00 13000.00 0.00 0.06784 20000.00 13000.00 0.00 0.06816 20200.00 13000.00 0.00 0.06834 19600.00 13200.00 0.00 0.06847 19800.00 13200.00 0.00 0.06917 20000.00 13200.00 0.00 0.06949 20200.00 13200.00 0.00 0.06961 19600.00 13400.00 0.00 0.06984 19800.00 13400.00 0.00 0.07055 20000.00 13400.00 0.00 0.07086 20200.00 13400.00 0.00 0.07094 19600.00 13600.00 0.00 0.07125 19800.00 13600.00 0.00 0.07202 20000.00 13600.00 0.00 0.07237 20200.00 13600.00 0.00 0.07246 19600.00 13800.00 0.00 0.07286 19800.00 13800.00 0.00 0.07366 20000.00 13800.00 0.00 0.07398 20200.00 13800.00 0.00 0.07404 19600.00 14000.00 0.00 0.07434 19800.00 14000.00 0.00 0.07507 20000.00 14000.00 0.00 0.07531 20200.00 14000.00 0.00 0.07532 19600.00 14200.00 0.00 0.07575 19800.00 14200.00 0.00 0.07646 20000.00 14200.00 0.00 0.07669 20200.00 14200.00 0.00 0.07675 19600.00 14400.00 0.00 0.07735 19800.00 14400.00 0.00 0.07807 20000.00 14400.00 0.00 0.07835 20200.00 14400.00 0.00 0.07843 19600.00 14600.00 0.00 0.07909 19800.00 14600.00 0.00 0.07978 20000.00 14600.00 0.00 0.08004 20200.00 14600.00 0.00 0.08009 19600.00 14800.00 0.00 0.08077 19800.00 14800.00 0.00 0.08145 20000.00 14800.00 0.00 0.08171 20200.00 14800.00 0.00 0.08173 19600.00 15000.00 0.00 0.08268 19800.00 15000.00 0.00 0.08337 20000.00 15000.00 0.00 0.08362 20200.00 15000.00 0.00 0.08362 19400.00 15200.00 0.00 0.08368 19600.00 15200.00 0.00 0.08481 19800.00 15200.00 0.00 0.08546 20000.00 15200.00 0.00 0.08565 19400.00 15400.00 0.00 0.08570 19600.00 15400.00 0.00 0.08677 19800.00 15400.00 0.00 0.08736 20000.00 15400.00 0.00 0.08751 19400.00 15600.00 0.00 0.08760 19600.00 15600.00 0.00 0.08863 19800.00 15600.00 0.00 0.08918 20000.00 15600.00 0.00 0.08934 19400.00 15800.00 0.00 0.08952 19600.00 15800.00 0.00 0.09051 19800.00 15800.00 0.00 0.09106 20000.00 15800.00 0.00 0.09122 19400.00 16000.00 0.00 0.09153 19600.00 16000.00 0.00 0.09249 19800.00 16000.00 0.00 0.09305 20000.00 16000.00 0.00 0.09322 19400.00 16200.00 0.00 0.09358 19600.00 16200.00 0.00 0.09455 19800.00 16200.00 0.00 0.09513 20000.00 16200.00 0.00 0.09525 19400.00 16400.00 0.00 0.09578 19600.00 16400.00 0.00 0.09673 19800.00 16400.00 0.00 0.09729 20000.00 16400.00 0.00 0.09735 19400.00 16600.00 0.00 0.09798 19600.00 16600.00 0.00 0.09892 19800.00 16600.00 0.00 0.09946 19400.00 16800.00 0.00 0.10033 19600.00 16800.00 0.00 0.10124 19800.00 16800.00 0.00 0.10173 19400.00 17000.00 0.00 0.10253 19600.00 17000.00 0.00 0.10339 19800.00 17000.00 0.00 0.10380 19400.00 17200.00 0.00 0.10460 19600.00 17200.00 0.00 0.10540 19800.00 17200.00 0.00 0.10570 19400.00 17400.00 0.00 0.10689 19600.00 17400.00 0.00 0.10757 19800.00 17400.00 0.00 0.10773 19400.00 17600.00 0.00 0.10910 19600.00 17600.00 0.00 0.10968 19800.00 17600.00 0.00 0.10972 19200.00 17800.00 0.00 0.10995 19400.00 17800.00 0.00 0.11167 19600.00 17800.00 0.00 0.11218 19200.00 18000.00 0.00 0.11265 19400.00 18000.00 0.00 0.11434 19600.00 18000.00 0.00 0.11475 19200.00 18200.00 0.00 0.11560 19400.00 18200.00 0.00 0.11723 19600.00 18200.00 0.00 0.11748 19200.00 18400.00 0.00 0.11868 19400.00 18400.00 0.00 0.12021 19600.00 18400.00 0.00 0.12025 19200.00 18600.00 0.00 0.12177 19400.00 18600.00 0.00 0.12313 19200.00 18800.00 0.00 0.12499 19400.00 18800.00 0.00 0.12611 19200.00 19000.00 0.00 0.12776 19400.00 19000.00 0.00 0.12858 19200.00 19200.00 0.00 0.13016 19400.00 19200.00 0.00 0.13033 19200.00 19400.00 15.00 0.13036 19200.00 19400.00 20.00 0.13041 19200.00 19400.00 25.00 0.13046 19200.00 19400.00 30.00 0.13051 19200.00 19400.00 35.00 0.13055 19200.00 19400.00 40.00 0.13058 19200.00 19400.00 45.00 0.13060 19200.00 19400.00 50.00 0.13060 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19250.41 19314.53 48.46 0.13087 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/545.ctf NX, NY, NZ: 512 512 1 MODE : real