CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/544.mrc Output diagnostic file name Micrographs_0601/544.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/544.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2460.000 7822.000 Mean, RMS : 4586.342 223.3172 TITLE 1: emi2mrc: 544.emi Mon Jan 06 18:35:29 2014 TITLE 2: kV 300, Mag 78000x, Def 21490, Spot 4, Int 55.04%, Obj 88.04%, Dif 73.67% TITLE 3: X 2.852, Y 284.732, Z -39.500, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00553 10200.00 10000.00 0.00 -0.00529 10400.00 10000.00 0.00 -0.00492 10600.00 10000.00 0.00 -0.00459 10800.00 10000.00 0.00 -0.00417 11000.00 10000.00 0.00 -0.00368 11200.00 10000.00 0.00 -0.00298 11400.00 10000.00 0.00 -0.00214 11600.00 10000.00 0.00 -0.00139 11800.00 10000.00 0.00 -0.00088 12000.00 10000.00 0.00 -0.00036 12200.00 10000.00 0.00 0.00037 12400.00 10000.00 0.00 0.00109 12600.00 10000.00 0.00 0.00190 12800.00 10000.00 0.00 0.00271 13000.00 10000.00 0.00 0.00347 13200.00 10000.00 0.00 0.00441 13400.00 10000.00 0.00 0.00534 13600.00 10000.00 0.00 0.00641 13800.00 10000.00 0.00 0.00767 14000.00 10000.00 0.00 0.00879 14200.00 10000.00 0.00 0.00998 14400.00 10000.00 0.00 0.01132 14600.00 10000.00 0.00 0.01263 14800.00 10000.00 0.00 0.01404 15000.00 10000.00 0.00 0.01550 15200.00 10000.00 0.00 0.01691 15400.00 10000.00 0.00 0.01834 15600.00 10000.00 0.00 0.02001 15800.00 10000.00 0.00 0.02182 16000.00 10000.00 0.00 0.02352 16200.00 10000.00 0.00 0.02522 16400.00 10000.00 0.00 0.02695 16600.00 10000.00 0.00 0.02875 16800.00 10000.00 0.00 0.03058 17000.00 10000.00 0.00 0.03245 17200.00 10000.00 0.00 0.03452 17400.00 10000.00 0.00 0.03669 17600.00 10000.00 0.00 0.03882 17800.00 10000.00 0.00 0.04103 18000.00 10000.00 0.00 0.04334 18200.00 10000.00 0.00 0.04575 18400.00 10000.00 0.00 0.04825 18600.00 10000.00 0.00 0.05054 18800.00 10000.00 0.00 0.05232 19000.00 10000.00 0.00 0.05339 19200.00 10000.00 0.00 0.05398 19400.00 10000.00 0.00 0.05423 19600.00 10000.00 0.00 0.05429 19800.00 10000.00 0.00 0.05430 19000.00 10200.00 0.00 0.05433 19200.00 10200.00 0.00 0.05489 19400.00 10200.00 0.00 0.05514 19600.00 10200.00 0.00 0.05523 19800.00 10200.00 0.00 0.05524 19000.00 10400.00 0.00 0.05546 19200.00 10400.00 0.00 0.05602 19400.00 10400.00 0.00 0.05628 19600.00 10400.00 0.00 0.05637 19000.00 10600.00 0.00 0.05660 19200.00 10600.00 0.00 0.05717 19400.00 10600.00 0.00 0.05745 19600.00 10600.00 0.00 0.05753 19000.00 10800.00 0.00 0.05763 19200.00 10800.00 0.00 0.05826 19400.00 10800.00 0.00 0.05856 19600.00 10800.00 0.00 0.05861 19000.00 11000.00 0.00 0.05889 19200.00 11000.00 0.00 0.05955 19400.00 11000.00 0.00 0.05980 19000.00 11200.00 0.00 0.06018 19200.00 11200.00 0.00 0.06079 19400.00 11200.00 0.00 0.06094 19000.00 11400.00 0.00 0.06138 19200.00 11400.00 0.00 0.06190 19400.00 11400.00 0.00 0.06199 19000.00 11600.00 0.00 0.06239 19200.00 11600.00 0.00 0.06285 19400.00 11600.00 0.00 0.06293 19000.00 11800.00 0.00 0.06353 19200.00 11800.00 0.00 0.06395 19400.00 11800.00 0.00 0.06402 19000.00 12000.00 0.00 0.06472 19200.00 12000.00 0.00 0.06511 19400.00 12000.00 0.00 0.06515 19000.00 12200.00 0.00 0.06583 19200.00 12200.00 0.00 0.06620 19400.00 12200.00 0.00 0.06622 19000.00 12400.00 0.00 0.06715 19200.00 12400.00 0.00 0.06748 19000.00 12600.00 0.00 0.06844 19200.00 12600.00 0.00 0.06871 18800.00 12800.00 0.00 0.06878 19000.00 12800.00 0.00 0.06973 19200.00 12800.00 0.00 0.06996 18800.00 13000.00 0.00 0.07006 19000.00 13000.00 0.00 0.07099 19200.00 13000.00 0.00 0.07118 18800.00 13200.00 0.00 0.07144 19000.00 13200.00 0.00 0.07235 19200.00 13200.00 0.00 0.07249 18800.00 13400.00 0.00 0.07299 19000.00 13400.00 0.00 0.07385 19200.00 13400.00 0.00 0.07394 18800.00 13600.00 0.00 0.07462 19000.00 13600.00 0.00 0.07540 19200.00 13600.00 0.00 0.07541 18800.00 13800.00 0.00 0.07621 19000.00 13800.00 0.00 0.07692 19200.00 13800.00 0.00 0.07693 18800.00 14000.00 0.00 0.07785 19000.00 14000.00 0.00 0.07855 19200.00 14000.00 0.00 0.07859 18800.00 14200.00 0.00 0.07975 19000.00 14200.00 0.00 0.08045 19200.00 14200.00 0.00 0.08051 18800.00 14400.00 0.00 0.08161 19000.00 14400.00 0.00 0.08230 19200.00 14400.00 0.00 0.08238 18800.00 14600.00 0.00 0.08360 19000.00 14600.00 0.00 0.08431 19200.00 14600.00 0.00 0.08439 18800.00 14800.00 0.00 0.08571 19000.00 14800.00 0.00 0.08643 19200.00 14800.00 0.00 0.08650 18800.00 15000.00 0.00 0.08792 19000.00 15000.00 0.00 0.08865 19200.00 15000.00 0.00 0.08872 18800.00 15200.00 0.00 0.09024 19000.00 15200.00 0.00 0.09100 19200.00 15200.00 0.00 0.09104 18800.00 15400.00 0.00 0.09261 19000.00 15400.00 0.00 0.09333 19200.00 15400.00 0.00 0.09335 18800.00 15600.00 0.00 0.09505 19000.00 15600.00 0.00 0.09577 18800.00 15800.00 0.00 0.09751 19000.00 15800.00 0.00 0.09820 18800.00 16000.00 0.00 0.10009 19000.00 16000.00 0.00 0.10069 18800.00 16200.00 0.00 0.10257 19000.00 16200.00 0.00 0.10312 18600.00 16400.00 0.00 0.10323 18800.00 16400.00 0.00 0.10529 19000.00 16400.00 0.00 0.10580 18600.00 16600.00 0.00 0.10603 18800.00 16600.00 0.00 0.10811 19000.00 16600.00 0.00 0.10853 18600.00 16800.00 0.00 0.10905 18800.00 16800.00 0.00 0.11109 19000.00 16800.00 0.00 0.11137 18600.00 17000.00 0.00 0.11240 18800.00 17000.00 0.00 0.11435 19000.00 17000.00 0.00 0.11446 18600.00 17200.00 0.00 0.11570 18800.00 17200.00 0.00 0.11751 18600.00 17400.00 0.00 0.11914 18800.00 17400.00 0.00 0.12080 18600.00 17600.00 0.00 0.12247 18800.00 17600.00 0.00 0.12396 18600.00 17800.00 0.00 0.12602 18800.00 17800.00 0.00 0.12733 18600.00 18000.00 0.00 0.12964 18800.00 18000.00 0.00 0.13071 18600.00 18200.00 0.00 0.13338 18800.00 18200.00 0.00 0.13403 18400.00 18400.00 0.00 0.13477 18600.00 18400.00 0.00 0.13694 18800.00 18400.00 0.00 0.13704 18400.00 18600.00 0.00 0.13774 18600.00 18600.00 0.00 0.13938 18600.00 18800.00 0.00 0.13981 18600.00 18800.00 5.00 0.13994 18600.00 18800.00 10.00 0.14005 18600.00 18800.00 15.00 0.14013 18600.00 18800.00 20.00 0.14020 18600.00 18800.00 25.00 0.14023 18600.00 18800.00 30.00 0.14024 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18558.40 18762.90 29.28 0.14039 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/544.ctf NX, NY, NZ: 512 512 1 MODE : real