CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/543.mrc Output diagnostic file name Micrographs_0601/543.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/543.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1918.000 8640.000 Mean, RMS : 4593.757 226.0432 TITLE 1: emi2mrc: 543.emi Mon Jan 06 18:33:47 2014 TITLE 2: kV 300, Mag 78000x, Def 21170, Spot 4, Int 55.04%, Obj 88.03%, Dif 73.67% TITLE 3: X 6.424, Y 280.132, Z -39.500, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01353 10200.00 10000.00 0.00 -0.01346 10400.00 10000.00 0.00 -0.01313 10600.00 10000.00 0.00 -0.01288 10800.00 10000.00 0.00 -0.01274 11000.00 10000.00 0.00 -0.01264 11200.00 10000.00 0.00 -0.01238 11400.00 10000.00 0.00 -0.01201 11600.00 10000.00 0.00 -0.01171 11800.00 10000.00 0.00 -0.01136 12000.00 10000.00 0.00 -0.01108 12200.00 10000.00 0.00 -0.01090 12400.00 10000.00 0.00 -0.01065 12600.00 10000.00 0.00 -0.01032 12800.00 10000.00 0.00 -0.00995 13000.00 10000.00 0.00 -0.00944 13200.00 10000.00 0.00 -0.00889 13400.00 10000.00 0.00 -0.00836 13600.00 10000.00 0.00 -0.00787 13800.00 10000.00 0.00 -0.00721 14000.00 10000.00 0.00 -0.00641 14200.00 10000.00 0.00 -0.00559 14400.00 10000.00 0.00 -0.00472 14600.00 10000.00 0.00 -0.00394 14800.00 10000.00 0.00 -0.00309 15000.00 10000.00 0.00 -0.00220 15200.00 10000.00 0.00 -0.00127 15400.00 10000.00 0.00 -0.00020 15600.00 10000.00 0.00 0.00077 15800.00 10000.00 0.00 0.00177 16000.00 10000.00 0.00 0.00281 16200.00 10000.00 0.00 0.00384 16400.00 10000.00 0.00 0.00490 16600.00 10000.00 0.00 0.00600 16800.00 10000.00 0.00 0.00720 17000.00 10000.00 0.00 0.00830 17200.00 10000.00 0.00 0.00938 17400.00 10000.00 0.00 0.01046 17600.00 10000.00 0.00 0.01146 17800.00 10000.00 0.00 0.01239 18000.00 10000.00 0.00 0.01334 18200.00 10000.00 0.00 0.01448 18400.00 10000.00 0.00 0.01556 18600.00 10000.00 0.00 0.01655 18800.00 10000.00 0.00 0.01774 19000.00 10000.00 0.00 0.01903 19200.00 10000.00 0.00 0.02037 19400.00 10000.00 0.00 0.02175 19600.00 10000.00 0.00 0.02301 19800.00 10000.00 0.00 0.02421 20000.00 10000.00 0.00 0.02560 20200.00 10000.00 0.00 0.02701 20400.00 10000.00 0.00 0.02830 20600.00 10000.00 0.00 0.02963 20800.00 10000.00 0.00 0.03093 21000.00 10000.00 0.00 0.03241 21200.00 10000.00 0.00 0.03414 21400.00 10000.00 0.00 0.03591 21600.00 10000.00 0.00 0.03739 21800.00 10000.00 0.00 0.03835 22000.00 10000.00 0.00 0.03905 22200.00 10000.00 0.00 0.03956 22400.00 10000.00 0.00 0.03982 22600.00 10000.00 0.00 0.03999 22800.00 10000.00 0.00 0.04031 23000.00 10000.00 0.00 0.04061 23200.00 10000.00 0.00 0.04069 23400.00 10000.00 0.00 0.04078 22800.00 10200.00 0.00 0.04091 23000.00 10200.00 0.00 0.04116 23200.00 10200.00 0.00 0.04120 23400.00 10200.00 0.00 0.04126 22800.00 10400.00 0.00 0.04147 23000.00 10400.00 0.00 0.04166 23200.00 10400.00 0.00 0.04167 23400.00 10400.00 0.00 0.04171 22600.00 10600.00 0.00 0.04179 22800.00 10600.00 0.00 0.04208 23000.00 10600.00 0.00 0.04221 23200.00 10600.00 0.00 0.04223 23400.00 10600.00 0.00 0.04227 22600.00 10800.00 0.00 0.04237 22800.00 10800.00 0.00 0.04263 23000.00 10800.00 0.00 0.04276 23200.00 10800.00 0.00 0.04280 23400.00 10800.00 0.00 0.04283 22600.00 11000.00 0.00 0.04296 22800.00 11000.00 0.00 0.04323 23000.00 11000.00 0.00 0.04340 23200.00 11000.00 0.00 0.04346 22600.00 11200.00 0.00 0.04366 22800.00 11200.00 0.00 0.04395 23000.00 11200.00 0.00 0.04412 23200.00 11200.00 0.00 0.04414 22400.00 11400.00 0.00 0.04430 22600.00 11400.00 0.00 0.04453 22800.00 11400.00 0.00 0.04482 23000.00 11400.00 0.00 0.04495 22200.00 11600.00 0.00 0.04495 22400.00 11600.00 0.00 0.04509 22600.00 11600.00 0.00 0.04533 22800.00 11600.00 0.00 0.04562 23000.00 11600.00 0.00 0.04570 22400.00 11800.00 0.00 0.04581 22600.00 11800.00 0.00 0.04609 22800.00 11800.00 0.00 0.04637 23000.00 11800.00 0.00 0.04640 22400.00 12000.00 0.00 0.04656 22600.00 12000.00 0.00 0.04690 22800.00 12000.00 0.00 0.04714 22200.00 12200.00 0.00 0.04716 22400.00 12200.00 0.00 0.04741 22600.00 12200.00 0.00 0.04776 22800.00 12200.00 0.00 0.04792 22200.00 12400.00 0.00 0.04805 22400.00 12400.00 0.00 0.04832 22600.00 12400.00 0.00 0.04861 22800.00 12400.00 0.00 0.04868 22200.00 12600.00 0.00 0.04890 22400.00 12600.00 0.00 0.04913 22600.00 12600.00 0.00 0.04934 22800.00 12600.00 0.00 0.04936 22200.00 12800.00 0.00 0.04972 22400.00 12800.00 0.00 0.04993 22600.00 12800.00 0.00 0.05010 22000.00 13000.00 0.00 0.05024 22200.00 13000.00 0.00 0.05060 22400.00 13000.00 0.00 0.05078 22600.00 13000.00 0.00 0.05092 22000.00 13200.00 0.00 0.05131 22200.00 13200.00 0.00 0.05163 22400.00 13200.00 0.00 0.05177 22600.00 13200.00 0.00 0.05188 22000.00 13400.00 0.00 0.05227 22200.00 13400.00 0.00 0.05255 22400.00 13400.00 0.00 0.05270 22600.00 13400.00 0.00 0.05285 22800.00 13400.00 0.00 0.05287 22000.00 13600.00 0.00 0.05323 22200.00 13600.00 0.00 0.05353 22400.00 13600.00 0.00 0.05374 22600.00 13600.00 0.00 0.05391 22800.00 13600.00 0.00 0.05392 22000.00 13800.00 0.00 0.05437 22200.00 13800.00 0.00 0.05469 22400.00 13800.00 0.00 0.05490 22600.00 13800.00 0.00 0.05501 22000.00 14000.00 0.00 0.05545 22200.00 14000.00 0.00 0.05575 22400.00 14000.00 0.00 0.05593 22600.00 14000.00 0.00 0.05601 22000.00 14200.00 0.00 0.05662 22200.00 14200.00 0.00 0.05693 22400.00 14200.00 0.00 0.05713 22600.00 14200.00 0.00 0.05723 22000.00 14400.00 0.00 0.05784 22200.00 14400.00 0.00 0.05817 22400.00 14400.00 0.00 0.05837 22600.00 14400.00 0.00 0.05844 22000.00 14600.00 0.00 0.05902 22200.00 14600.00 0.00 0.05936 22400.00 14600.00 0.00 0.05952 22600.00 14600.00 0.00 0.05953 22000.00 14800.00 0.00 0.06015 22200.00 14800.00 0.00 0.06048 22400.00 14800.00 0.00 0.06062 21800.00 15000.00 0.00 0.06063 22000.00 15000.00 0.00 0.06135 22200.00 15000.00 0.00 0.06167 22400.00 15000.00 0.00 0.06179 21800.00 15200.00 0.00 0.06196 22000.00 15200.00 0.00 0.06267 22200.00 15200.00 0.00 0.06296 22400.00 15200.00 0.00 0.06305 21800.00 15400.00 0.00 0.06334 22000.00 15400.00 0.00 0.06401 22200.00 15400.00 0.00 0.06426 22400.00 15400.00 0.00 0.06432 21800.00 15600.00 0.00 0.06468 22000.00 15600.00 0.00 0.06534 22200.00 15600.00 0.00 0.06559 22400.00 15600.00 0.00 0.06563 21800.00 15800.00 0.00 0.06609 22000.00 15800.00 0.00 0.06678 22200.00 15800.00 0.00 0.06702 22400.00 15800.00 0.00 0.06706 21800.00 16000.00 0.00 0.06772 22000.00 16000.00 0.00 0.06841 22200.00 16000.00 0.00 0.06863 22400.00 16000.00 0.00 0.06864 21800.00 16200.00 0.00 0.06945 22000.00 16200.00 0.00 0.07010 22200.00 16200.00 0.00 0.07027 21800.00 16400.00 0.00 0.07130 22000.00 16400.00 0.00 0.07189 22200.00 16400.00 0.00 0.07203 21800.00 16600.00 0.00 0.07300 22000.00 16600.00 0.00 0.07356 22200.00 16600.00 0.00 0.07367 21600.00 16800.00 0.00 0.07367 21800.00 16800.00 0.00 0.07470 22000.00 16800.00 0.00 0.07524 22200.00 16800.00 0.00 0.07530 21600.00 17000.00 0.00 0.07537 21800.00 17000.00 0.00 0.07640 22000.00 17000.00 0.00 0.07687 22200.00 17000.00 0.00 0.07687 21600.00 17200.00 0.00 0.07693 21800.00 17200.00 0.00 0.07792 22000.00 17200.00 0.00 0.07832 21600.00 17400.00 0.00 0.07856 21800.00 17400.00 0.00 0.07952 22000.00 17400.00 0.00 0.07987 21600.00 17600.00 0.00 0.08017 21800.00 17600.00 0.00 0.08110 22000.00 17600.00 0.00 0.08141 21600.00 17800.00 0.00 0.08194 21800.00 17800.00 0.00 0.08281 22000.00 17800.00 0.00 0.08304 21600.00 18000.00 0.00 0.08359 21800.00 18000.00 0.00 0.08439 22000.00 18000.00 0.00 0.08455 21600.00 18200.00 0.00 0.08528 21800.00 18200.00 0.00 0.08601 22000.00 18200.00 0.00 0.08611 21600.00 18400.00 0.00 0.08715 21800.00 18400.00 0.00 0.08780 22000.00 18400.00 0.00 0.08785 21600.00 18600.00 0.00 0.08903 21800.00 18600.00 0.00 0.08963 22000.00 18600.00 0.00 0.08967 21600.00 18800.00 0.00 0.09111 21800.00 18800.00 0.00 0.09168 22000.00 18800.00 0.00 0.09171 21600.00 19000.00 0.00 0.09309 21800.00 19000.00 0.00 0.09366 22000.00 19000.00 0.00 0.09367 21600.00 19200.00 0.00 0.09518 21800.00 19200.00 0.00 0.09574 21400.00 19400.00 0.00 0.09575 21600.00 19400.00 0.00 0.09735 21800.00 19400.00 0.00 0.09787 21400.00 19600.00 0.00 0.09804 21600.00 19600.00 0.00 0.09963 21800.00 19600.00 0.00 0.10004 21400.00 19800.00 0.00 0.10033 21600.00 19800.00 0.00 0.10188 21800.00 19800.00 0.00 0.10218 21400.00 20000.00 0.00 0.10265 21600.00 20000.00 0.00 0.10416 21800.00 20000.00 0.00 0.10435 21400.00 20200.00 0.00 0.10512 21600.00 20200.00 0.00 0.10656 21800.00 20200.00 0.00 0.10660 21400.00 20400.00 0.00 0.10752 21600.00 20400.00 0.00 0.10884 21400.00 20600.00 0.00 0.11006 21600.00 20600.00 0.00 0.11126 21400.00 20800.00 0.00 0.11256 21600.00 20800.00 0.00 0.11367 21400.00 21000.00 0.00 0.11525 21600.00 21000.00 0.00 0.11620 21400.00 21200.00 0.00 0.11764 21600.00 21200.00 0.00 0.11832 21400.00 21400.00 0.00 0.11956 21600.00 21400.00 0.00 0.11977 21400.00 21600.00 0.00 0.11999 21400.00 21600.00 5.00 0.12006 21400.00 21600.00 10.00 0.12012 21400.00 21600.00 15.00 0.12017 21400.00 21600.00 20.00 0.12021 21400.00 21600.00 25.00 0.12024 21400.00 21600.00 30.00 0.12026 21400.00 21600.00 35.00 0.12027 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21441.53 21546.16 35.98 0.12038 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/543.ctf NX, NY, NZ: 512 512 1 MODE : real