CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/541.mrc Output diagnostic file name Micrographs_0601/541.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/541.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2058.000 8308.000 Mean, RMS : 4555.775 215.8324 TITLE 1: emi2mrc: 541.emi Mon Jan 06 18:30:32 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -9.433, Y 288.417, Z -39.500, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00866 10200.00 10000.00 0.00 -0.00837 10400.00 10000.00 0.00 -0.00798 10600.00 10000.00 0.00 -0.00756 10800.00 10000.00 0.00 -0.00723 11000.00 10000.00 0.00 -0.00686 11200.00 10000.00 0.00 -0.00631 11400.00 10000.00 0.00 -0.00585 11600.00 10000.00 0.00 -0.00537 11800.00 10000.00 0.00 -0.00488 12000.00 10000.00 0.00 -0.00452 12200.00 10000.00 0.00 -0.00399 12400.00 10000.00 0.00 -0.00340 12600.00 10000.00 0.00 -0.00276 12800.00 10000.00 0.00 -0.00193 13000.00 10000.00 0.00 -0.00102 13200.00 10000.00 0.00 -0.00016 13400.00 10000.00 0.00 0.00067 13600.00 10000.00 0.00 0.00173 13800.00 10000.00 0.00 0.00293 14000.00 10000.00 0.00 0.00411 14200.00 10000.00 0.00 0.00530 14400.00 10000.00 0.00 0.00656 14600.00 10000.00 0.00 0.00789 14800.00 10000.00 0.00 0.00909 15000.00 10000.00 0.00 0.01042 15200.00 10000.00 0.00 0.01195 15400.00 10000.00 0.00 0.01331 15600.00 10000.00 0.00 0.01458 15800.00 10000.00 0.00 0.01585 16000.00 10000.00 0.00 0.01718 16200.00 10000.00 0.00 0.01874 16400.00 10000.00 0.00 0.02038 16600.00 10000.00 0.00 0.02183 16800.00 10000.00 0.00 0.02303 17000.00 10000.00 0.00 0.02435 17200.00 10000.00 0.00 0.02591 17400.00 10000.00 0.00 0.02768 17600.00 10000.00 0.00 0.02950 17800.00 10000.00 0.00 0.03130 18000.00 10000.00 0.00 0.03323 18200.00 10000.00 0.00 0.03520 18400.00 10000.00 0.00 0.03711 18600.00 10000.00 0.00 0.03906 18800.00 10000.00 0.00 0.04110 19000.00 10000.00 0.00 0.04298 19200.00 10000.00 0.00 0.04440 19400.00 10000.00 0.00 0.04529 19600.00 10000.00 0.00 0.04592 19800.00 10000.00 0.00 0.04638 20000.00 10000.00 0.00 0.04662 20200.00 10000.00 0.00 0.04690 20400.00 10000.00 0.00 0.04717 20600.00 10000.00 0.00 0.04739 20800.00 10000.00 0.00 0.04758 21000.00 10000.00 0.00 0.04763 21200.00 10000.00 0.00 0.04764 20200.00 10200.00 0.00 0.04767 20400.00 10200.00 0.00 0.04791 20600.00 10200.00 0.00 0.04812 20800.00 10200.00 0.00 0.04826 20200.00 10400.00 0.00 0.04853 20400.00 10400.00 0.00 0.04872 20600.00 10400.00 0.00 0.04886 20800.00 10400.00 0.00 0.04893 20000.00 10600.00 0.00 0.04904 20200.00 10600.00 0.00 0.04925 20400.00 10600.00 0.00 0.04935 20600.00 10600.00 0.00 0.04945 20800.00 10600.00 0.00 0.04948 20000.00 10800.00 0.00 0.04969 20200.00 10800.00 0.00 0.04987 20400.00 10800.00 0.00 0.04994 20600.00 10800.00 0.00 0.05006 20800.00 10800.00 0.00 0.05011 19800.00 11000.00 0.00 0.05023 20000.00 11000.00 0.00 0.05049 20200.00 11000.00 0.00 0.05065 20400.00 11000.00 0.00 0.05073 20600.00 11000.00 0.00 0.05089 20800.00 11000.00 0.00 0.05096 21000.00 11000.00 0.00 0.05097 21200.00 11000.00 0.00 0.05098 19800.00 11200.00 0.00 0.05101 20000.00 11200.00 0.00 0.05127 20200.00 11200.00 0.00 0.05141 20400.00 11200.00 0.00 0.05151 20600.00 11200.00 0.00 0.05171 20800.00 11200.00 0.00 0.05179 21000.00 11200.00 0.00 0.05181 19800.00 11400.00 0.00 0.05208 20000.00 11400.00 0.00 0.05234 20200.00 11400.00 0.00 0.05249 20400.00 11400.00 0.00 0.05264 20600.00 11400.00 0.00 0.05288 20800.00 11400.00 0.00 0.05296 19800.00 11600.00 0.00 0.05328 20000.00 11600.00 0.00 0.05355 20200.00 11600.00 0.00 0.05368 20400.00 11600.00 0.00 0.05387 20600.00 11600.00 0.00 0.05410 20800.00 11600.00 0.00 0.05410 19600.00 11800.00 0.00 0.05417 19800.00 11800.00 0.00 0.05455 20000.00 11800.00 0.00 0.05477 20200.00 11800.00 0.00 0.05489 20400.00 11800.00 0.00 0.05507 20600.00 11800.00 0.00 0.05524 19600.00 12000.00 0.00 0.05540 19800.00 12000.00 0.00 0.05572 20000.00 12000.00 0.00 0.05594 20200.00 12000.00 0.00 0.05608 20400.00 12000.00 0.00 0.05627 20600.00 12000.00 0.00 0.05637 19600.00 12200.00 0.00 0.05650 19800.00 12200.00 0.00 0.05683 20000.00 12200.00 0.00 0.05710 20200.00 12200.00 0.00 0.05725 20400.00 12200.00 0.00 0.05740 20600.00 12200.00 0.00 0.05743 19600.00 12400.00 0.00 0.05755 19800.00 12400.00 0.00 0.05794 20000.00 12400.00 0.00 0.05823 20200.00 12400.00 0.00 0.05835 20400.00 12400.00 0.00 0.05844 19600.00 12600.00 0.00 0.05858 19800.00 12600.00 0.00 0.05902 20000.00 12600.00 0.00 0.05931 20200.00 12600.00 0.00 0.05939 20400.00 12600.00 0.00 0.05946 19400.00 12800.00 0.00 0.05950 19600.00 12800.00 0.00 0.05999 19800.00 12800.00 0.00 0.06043 20000.00 12800.00 0.00 0.06068 20200.00 12800.00 0.00 0.06072 20400.00 12800.00 0.00 0.06076 19400.00 13000.00 0.00 0.06085 19600.00 13000.00 0.00 0.06132 19800.00 13000.00 0.00 0.06175 20000.00 13000.00 0.00 0.06195 20200.00 13000.00 0.00 0.06195 19400.00 13200.00 0.00 0.06222 19600.00 13200.00 0.00 0.06266 19800.00 13200.00 0.00 0.06306 20000.00 13200.00 0.00 0.06323 19400.00 13400.00 0.00 0.06369 19600.00 13400.00 0.00 0.06408 19800.00 13400.00 0.00 0.06447 20000.00 13400.00 0.00 0.06460 19400.00 13600.00 0.00 0.06525 19600.00 13600.00 0.00 0.06561 19800.00 13600.00 0.00 0.06601 20000.00 13600.00 0.00 0.06610 19400.00 13800.00 0.00 0.06679 19600.00 13800.00 0.00 0.06717 19800.00 13800.00 0.00 0.06757 20000.00 13800.00 0.00 0.06757 19400.00 14000.00 0.00 0.06819 19600.00 14000.00 0.00 0.06855 19800.00 14000.00 0.00 0.06889 19200.00 14200.00 0.00 0.06899 19400.00 14200.00 0.00 0.06973 19600.00 14200.00 0.00 0.07006 19800.00 14200.00 0.00 0.07032 19200.00 14400.00 0.00 0.07055 19400.00 14400.00 0.00 0.07124 19600.00 14400.00 0.00 0.07154 19800.00 14400.00 0.00 0.07176 19200.00 14600.00 0.00 0.07231 19400.00 14600.00 0.00 0.07293 19600.00 14600.00 0.00 0.07322 19800.00 14600.00 0.00 0.07343 19200.00 14800.00 0.00 0.07406 19400.00 14800.00 0.00 0.07467 19600.00 14800.00 0.00 0.07498 19800.00 14800.00 0.00 0.07519 19200.00 15000.00 0.00 0.07577 19400.00 15000.00 0.00 0.07641 19600.00 15000.00 0.00 0.07678 19800.00 15000.00 0.00 0.07698 19200.00 15200.00 0.00 0.07762 19400.00 15200.00 0.00 0.07834 19600.00 15200.00 0.00 0.07873 19800.00 15200.00 0.00 0.07886 19200.00 15400.00 0.00 0.07965 19400.00 15400.00 0.00 0.08040 19600.00 15400.00 0.00 0.08074 19800.00 15400.00 0.00 0.08078 19200.00 15600.00 0.00 0.08180 19400.00 15600.00 0.00 0.08250 19600.00 15600.00 0.00 0.08274 19200.00 15800.00 0.00 0.08368 19400.00 15800.00 0.00 0.08432 19600.00 15800.00 0.00 0.08448 19200.00 16000.00 0.00 0.08560 19400.00 16000.00 0.00 0.08621 19600.00 16000.00 0.00 0.08631 19200.00 16200.00 0.00 0.08750 19400.00 16200.00 0.00 0.08806 19600.00 16200.00 0.00 0.08809 19000.00 16400.00 0.00 0.08814 19200.00 16400.00 0.00 0.08938 19400.00 16400.00 0.00 0.08990 19000.00 16600.00 0.00 0.09015 19200.00 16600.00 0.00 0.09138 19400.00 16600.00 0.00 0.09188 19000.00 16800.00 0.00 0.09220 19200.00 16800.00 0.00 0.09341 19400.00 16800.00 0.00 0.09386 19000.00 17000.00 0.00 0.09433 19200.00 17000.00 0.00 0.09551 19400.00 17000.00 0.00 0.09589 19000.00 17200.00 0.00 0.09660 19200.00 17200.00 0.00 0.09772 19400.00 17200.00 0.00 0.09805 19000.00 17400.00 0.00 0.09903 19200.00 17400.00 0.00 0.10011 19400.00 17400.00 0.00 0.10037 19000.00 17600.00 0.00 0.10142 19200.00 17600.00 0.00 0.10249 19400.00 17600.00 0.00 0.10270 19000.00 17800.00 0.00 0.10413 19200.00 17800.00 0.00 0.10518 19400.00 17800.00 0.00 0.10528 19000.00 18000.00 0.00 0.10680 19200.00 18000.00 0.00 0.10780 19000.00 18200.00 0.00 0.10956 19200.00 18200.00 0.00 0.11040 19000.00 18400.00 0.00 0.11236 19200.00 18400.00 0.00 0.11296 18800.00 18600.00 0.00 0.11342 19000.00 18600.00 0.00 0.11518 19200.00 18600.00 0.00 0.11534 18800.00 18800.00 0.00 0.11629 19000.00 18800.00 0.00 0.11740 19000.00 19000.00 0.00 0.11762 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18903.33 19001.38 48.68 0.11783 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/541.ctf NX, NY, NZ: 512 512 1 MODE : real