CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/540.mrc Output diagnostic file name Micrographs_0601/540.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/540.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2494.000 8164.000 Mean, RMS : 4553.885 223.3133 TITLE 1: emi2mrc: 540.emi Mon Jan 06 17:08:58 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 33.295, Y 488.738, Z -32.881, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00939 10400.00 10000.00 0.00 -0.00927 10600.00 10000.00 0.00 -0.00914 10800.00 10000.00 0.00 -0.00911 11000.00 10000.00 0.00 -0.00903 11200.00 10000.00 0.00 -0.00881 11400.00 10000.00 0.00 -0.00853 11600.00 10000.00 0.00 -0.00814 11800.00 10000.00 0.00 -0.00776 12000.00 10000.00 0.00 -0.00742 12200.00 10000.00 0.00 -0.00700 12400.00 10000.00 0.00 -0.00659 12600.00 10000.00 0.00 -0.00609 12800.00 10000.00 0.00 -0.00547 13000.00 10000.00 0.00 -0.00493 13200.00 10000.00 0.00 -0.00433 13400.00 10000.00 0.00 -0.00355 13600.00 10000.00 0.00 -0.00273 13800.00 10000.00 0.00 -0.00184 14000.00 10000.00 0.00 -0.00086 14200.00 10000.00 0.00 0.00003 14400.00 10000.00 0.00 0.00086 14600.00 10000.00 0.00 0.00178 14800.00 10000.00 0.00 0.00271 15000.00 10000.00 0.00 0.00350 15200.00 10000.00 0.00 0.00429 15400.00 10000.00 0.00 0.00517 15600.00 10000.00 0.00 0.00605 15800.00 10000.00 0.00 0.00698 16000.00 10000.00 0.00 0.00800 16200.00 10000.00 0.00 0.00911 16400.00 10000.00 0.00 0.01023 16600.00 10000.00 0.00 0.01129 16800.00 10000.00 0.00 0.01251 17000.00 10000.00 0.00 0.01386 17200.00 10000.00 0.00 0.01511 17400.00 10000.00 0.00 0.01650 17600.00 10000.00 0.00 0.01790 17800.00 10000.00 0.00 0.01917 18000.00 10000.00 0.00 0.02045 18200.00 10000.00 0.00 0.02174 18400.00 10000.00 0.00 0.02303 18600.00 10000.00 0.00 0.02417 18800.00 10000.00 0.00 0.02534 19000.00 10000.00 0.00 0.02674 19200.00 10000.00 0.00 0.02835 19400.00 10000.00 0.00 0.03004 19600.00 10000.00 0.00 0.03171 19800.00 10000.00 0.00 0.03354 20000.00 10000.00 0.00 0.03550 20200.00 10000.00 0.00 0.03757 20400.00 10000.00 0.00 0.03977 20600.00 10000.00 0.00 0.04205 20800.00 10000.00 0.00 0.04413 21000.00 10000.00 0.00 0.04558 21200.00 10000.00 0.00 0.04628 21400.00 10000.00 0.00 0.04650 21600.00 10000.00 0.00 0.04665 21200.00 10200.00 0.00 0.04674 21400.00 10200.00 0.00 0.04702 21600.00 10200.00 0.00 0.04723 21200.00 10400.00 0.00 0.04741 21400.00 10400.00 0.00 0.04775 21600.00 10400.00 0.00 0.04796 21200.00 10600.00 0.00 0.04826 21400.00 10600.00 0.00 0.04861 21600.00 10600.00 0.00 0.04876 21200.00 10800.00 0.00 0.04913 21400.00 10800.00 0.00 0.04945 21600.00 10800.00 0.00 0.04956 21200.00 11000.00 0.00 0.05000 21400.00 11000.00 0.00 0.05029 21600.00 11000.00 0.00 0.05038 21200.00 11200.00 0.00 0.05085 21400.00 11200.00 0.00 0.05110 21600.00 11200.00 0.00 0.05117 21200.00 11400.00 0.00 0.05174 21400.00 11400.00 0.00 0.05194 21600.00 11400.00 0.00 0.05198 21200.00 11600.00 0.00 0.05264 21400.00 11600.00 0.00 0.05281 21600.00 11600.00 0.00 0.05283 21000.00 11800.00 0.00 0.05291 21200.00 11800.00 0.00 0.05357 21400.00 11800.00 0.00 0.05371 21600.00 11800.00 0.00 0.05374 21000.00 12000.00 0.00 0.05382 21200.00 12000.00 0.00 0.05444 21400.00 12000.00 0.00 0.05457 21600.00 12000.00 0.00 0.05461 21000.00 12200.00 0.00 0.05476 21200.00 12200.00 0.00 0.05536 21400.00 12200.00 0.00 0.05549 21600.00 12200.00 0.00 0.05550 21000.00 12400.00 0.00 0.05567 21200.00 12400.00 0.00 0.05625 21400.00 12400.00 0.00 0.05634 21000.00 12600.00 0.00 0.05652 21200.00 12600.00 0.00 0.05709 21400.00 12600.00 0.00 0.05717 21000.00 12800.00 0.00 0.05758 21200.00 12800.00 0.00 0.05815 21400.00 12800.00 0.00 0.05820 21000.00 13000.00 0.00 0.05856 21200.00 13000.00 0.00 0.05909 21400.00 13000.00 0.00 0.05911 21000.00 13200.00 0.00 0.05955 21200.00 13200.00 0.00 0.06006 21400.00 13200.00 0.00 0.06009 21600.00 13200.00 0.00 0.06009 21000.00 13400.00 0.00 0.06058 21200.00 13400.00 0.00 0.06110 21400.00 13400.00 0.00 0.06118 21600.00 13400.00 0.00 0.06124 21000.00 13600.00 0.00 0.06165 21200.00 13600.00 0.00 0.06220 21400.00 13600.00 0.00 0.06230 21600.00 13600.00 0.00 0.06236 21000.00 13800.00 0.00 0.06281 21200.00 13800.00 0.00 0.06336 21400.00 13800.00 0.00 0.06345 21600.00 13800.00 0.00 0.06346 21000.00 14000.00 0.00 0.06400 21200.00 14000.00 0.00 0.06453 21400.00 14000.00 0.00 0.06459 21600.00 14000.00 0.00 0.06460 21000.00 14200.00 0.00 0.06527 21200.00 14200.00 0.00 0.06579 21400.00 14200.00 0.00 0.06587 21600.00 14200.00 0.00 0.06590 21000.00 14400.00 0.00 0.06642 21200.00 14400.00 0.00 0.06695 21400.00 14400.00 0.00 0.06701 21000.00 14600.00 0.00 0.06767 21200.00 14600.00 0.00 0.06818 21400.00 14600.00 0.00 0.06823 21000.00 14800.00 0.00 0.06907 21200.00 14800.00 0.00 0.06957 21400.00 14800.00 0.00 0.06965 21600.00 14800.00 0.00 0.06966 21000.00 15000.00 0.00 0.07070 21200.00 15000.00 0.00 0.07122 21400.00 15000.00 0.00 0.07133 21600.00 15000.00 0.00 0.07136 21000.00 15200.00 0.00 0.07241 21200.00 15200.00 0.00 0.07293 21400.00 15200.00 0.00 0.07305 21000.00 15400.00 0.00 0.07394 21200.00 15400.00 0.00 0.07447 21400.00 15400.00 0.00 0.07456 21000.00 15600.00 0.00 0.07555 21200.00 15600.00 0.00 0.07607 21400.00 15600.00 0.00 0.07615 21000.00 15800.00 0.00 0.07718 21200.00 15800.00 0.00 0.07770 21400.00 15800.00 0.00 0.07777 21000.00 16000.00 0.00 0.07880 21200.00 16000.00 0.00 0.07933 21400.00 16000.00 0.00 0.07938 21000.00 16200.00 0.00 0.08036 21200.00 16200.00 0.00 0.08090 21400.00 16200.00 0.00 0.08093 21000.00 16400.00 0.00 0.08217 21200.00 16400.00 0.00 0.08269 21000.00 16600.00 0.00 0.08412 21200.00 16600.00 0.00 0.08461 21000.00 16800.00 0.00 0.08606 21200.00 16800.00 0.00 0.08652 20800.00 17000.00 0.00 0.08653 21000.00 17000.00 0.00 0.08801 21200.00 17000.00 0.00 0.08841 21000.00 17200.00 0.00 0.08975 21200.00 17200.00 0.00 0.09013 21000.00 17400.00 0.00 0.09156 21200.00 17400.00 0.00 0.09197 20800.00 17600.00 0.00 0.09202 21000.00 17600.00 0.00 0.09349 21200.00 17600.00 0.00 0.09390 20800.00 17800.00 0.00 0.09415 21000.00 17800.00 0.00 0.09561 21200.00 17800.00 0.00 0.09598 20800.00 18000.00 0.00 0.09627 21000.00 18000.00 0.00 0.09774 21200.00 18000.00 0.00 0.09808 20800.00 18200.00 0.00 0.09853 21000.00 18200.00 0.00 0.10002 21200.00 18200.00 0.00 0.10033 20800.00 18400.00 0.00 0.10097 21000.00 18400.00 0.00 0.10243 21200.00 18400.00 0.00 0.10263 20800.00 18600.00 0.00 0.10330 21000.00 18600.00 0.00 0.10472 21200.00 18600.00 0.00 0.10480 20800.00 18800.00 0.00 0.10578 21000.00 18800.00 0.00 0.10716 20800.00 19000.00 0.00 0.10846 21000.00 19000.00 0.00 0.10982 20800.00 19200.00 0.00 0.11148 21000.00 19200.00 0.00 0.11272 20800.00 19400.00 0.00 0.11442 21000.00 19400.00 0.00 0.11546 20800.00 19600.00 0.00 0.11747 21000.00 19600.00 0.00 0.11829 20800.00 19800.00 0.00 0.12060 21000.00 19800.00 0.00 0.12126 20800.00 20000.00 0.00 0.12388 21000.00 20000.00 0.00 0.12432 20600.00 20200.00 0.00 0.12459 20800.00 20200.00 0.00 0.12723 21000.00 20200.00 0.00 0.12732 20600.00 20400.00 0.00 0.12800 20800.00 20400.00 0.00 0.13048 20600.00 20600.00 0.00 0.13210 20800.00 20600.00 0.00 0.13395 20600.00 20800.00 0.00 0.13443 20800.00 20800.00 0.00 0.13505 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20701.14 20826.09 36.49 0.13550 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/540.ctf NX, NY, NZ: 512 512 1 MODE : real