CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/539.mrc Output diagnostic file name Micrographs_0601/539.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/539.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2388.000 8362.000 Mean, RMS : 4554.405 217.5838 TITLE 1: emi2mrc: 539.emi Mon Jan 06 18:28:38 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.04%, Obj 88.06%, Dif 73.67% TITLE 3: X -11.282, Y 286.593, Z -39.500, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00883 10200.00 10000.00 0.00 -0.00846 10400.00 10000.00 0.00 -0.00794 10600.00 10000.00 0.00 -0.00745 10800.00 10000.00 0.00 -0.00693 11000.00 10000.00 0.00 -0.00636 11200.00 10000.00 0.00 -0.00560 11400.00 10000.00 0.00 -0.00488 11600.00 10000.00 0.00 -0.00432 11800.00 10000.00 0.00 -0.00369 12000.00 10000.00 0.00 -0.00303 12200.00 10000.00 0.00 -0.00233 12400.00 10000.00 0.00 -0.00161 12600.00 10000.00 0.00 -0.00088 12800.00 10000.00 0.00 -0.00009 13000.00 10000.00 0.00 0.00076 13200.00 10000.00 0.00 0.00177 13400.00 10000.00 0.00 0.00292 13600.00 10000.00 0.00 0.00404 13800.00 10000.00 0.00 0.00516 14000.00 10000.00 0.00 0.00632 14200.00 10000.00 0.00 0.00741 14400.00 10000.00 0.00 0.00864 14600.00 10000.00 0.00 0.00986 14800.00 10000.00 0.00 0.01092 15000.00 10000.00 0.00 0.01214 15200.00 10000.00 0.00 0.01342 15400.00 10000.00 0.00 0.01463 15600.00 10000.00 0.00 0.01602 15800.00 10000.00 0.00 0.01751 16000.00 10000.00 0.00 0.01902 16200.00 10000.00 0.00 0.02053 16400.00 10000.00 0.00 0.02204 16600.00 10000.00 0.00 0.02358 16800.00 10000.00 0.00 0.02498 17000.00 10000.00 0.00 0.02637 17200.00 10000.00 0.00 0.02785 17400.00 10000.00 0.00 0.02947 17600.00 10000.00 0.00 0.03112 17800.00 10000.00 0.00 0.03283 18000.00 10000.00 0.00 0.03489 18200.00 10000.00 0.00 0.03690 18400.00 10000.00 0.00 0.03875 18600.00 10000.00 0.00 0.04071 18800.00 10000.00 0.00 0.04256 19000.00 10000.00 0.00 0.04416 19200.00 10000.00 0.00 0.04553 19400.00 10000.00 0.00 0.04653 19600.00 10000.00 0.00 0.04722 19800.00 10000.00 0.00 0.04767 20000.00 10000.00 0.00 0.04786 20200.00 10000.00 0.00 0.04800 20400.00 10000.00 0.00 0.04804 19800.00 10200.00 0.00 0.04841 20000.00 10200.00 0.00 0.04862 20200.00 10200.00 0.00 0.04882 20400.00 10200.00 0.00 0.04891 19600.00 10400.00 0.00 0.04893 19800.00 10400.00 0.00 0.04937 20000.00 10400.00 0.00 0.04966 20200.00 10400.00 0.00 0.04992 20400.00 10400.00 0.00 0.05001 19800.00 10600.00 0.00 0.05046 20000.00 10600.00 0.00 0.05078 20200.00 10600.00 0.00 0.05101 20400.00 10600.00 0.00 0.05104 19800.00 10800.00 0.00 0.05143 20000.00 10800.00 0.00 0.05173 20200.00 10800.00 0.00 0.05191 19600.00 11000.00 0.00 0.05201 19800.00 11000.00 0.00 0.05253 20000.00 11000.00 0.00 0.05282 20200.00 11000.00 0.00 0.05296 19600.00 11200.00 0.00 0.05320 19800.00 11200.00 0.00 0.05371 20000.00 11200.00 0.00 0.05394 20200.00 11200.00 0.00 0.05400 19600.00 11400.00 0.00 0.05425 19800.00 11400.00 0.00 0.05468 20000.00 11400.00 0.00 0.05481 20200.00 11400.00 0.00 0.05482 19600.00 11600.00 0.00 0.05522 19800.00 11600.00 0.00 0.05559 20000.00 11600.00 0.00 0.05572 20200.00 11600.00 0.00 0.05577 19400.00 11800.00 0.00 0.05578 19600.00 11800.00 0.00 0.05637 19800.00 11800.00 0.00 0.05672 20000.00 11800.00 0.00 0.05689 20200.00 11800.00 0.00 0.05697 19400.00 12000.00 0.00 0.05699 19600.00 12000.00 0.00 0.05755 19800.00 12000.00 0.00 0.05790 20000.00 12000.00 0.00 0.05809 20200.00 12000.00 0.00 0.05817 19600.00 12200.00 0.00 0.05871 19800.00 12200.00 0.00 0.05908 20000.00 12200.00 0.00 0.05930 20200.00 12200.00 0.00 0.05935 19600.00 12400.00 0.00 0.05991 19800.00 12400.00 0.00 0.06033 20000.00 12400.00 0.00 0.06056 20200.00 12400.00 0.00 0.06056 19400.00 12600.00 0.00 0.06074 19600.00 12600.00 0.00 0.06135 19800.00 12600.00 0.00 0.06180 20000.00 12600.00 0.00 0.06199 19400.00 12800.00 0.00 0.06235 19600.00 12800.00 0.00 0.06297 19800.00 12800.00 0.00 0.06336 20000.00 12800.00 0.00 0.06347 19400.00 13000.00 0.00 0.06385 19600.00 13000.00 0.00 0.06440 19800.00 13000.00 0.00 0.06472 20000.00 13000.00 0.00 0.06476 19400.00 13200.00 0.00 0.06528 19600.00 13200.00 0.00 0.06580 19800.00 13200.00 0.00 0.06609 20000.00 13200.00 0.00 0.06611 19400.00 13400.00 0.00 0.06658 19600.00 13400.00 0.00 0.06715 19800.00 13400.00 0.00 0.06746 20000.00 13400.00 0.00 0.06748 19400.00 13600.00 0.00 0.06806 19600.00 13600.00 0.00 0.06864 19800.00 13600.00 0.00 0.06890 20000.00 13600.00 0.00 0.06890 19400.00 13800.00 0.00 0.06949 19600.00 13800.00 0.00 0.07000 19800.00 13800.00 0.00 0.07022 19400.00 14000.00 0.00 0.07091 19600.00 14000.00 0.00 0.07138 19800.00 14000.00 0.00 0.07162 20000.00 14000.00 0.00 0.07163 19200.00 14200.00 0.00 0.07169 19400.00 14200.00 0.00 0.07263 19600.00 14200.00 0.00 0.07311 19800.00 14200.00 0.00 0.07338 19400.00 14400.00 0.00 0.07429 19600.00 14400.00 0.00 0.07477 19800.00 14400.00 0.00 0.07500 19200.00 14600.00 0.00 0.07512 19400.00 14600.00 0.00 0.07605 19600.00 14600.00 0.00 0.07647 19800.00 14600.00 0.00 0.07659 19200.00 14800.00 0.00 0.07672 19400.00 14800.00 0.00 0.07762 19600.00 14800.00 0.00 0.07796 19800.00 14800.00 0.00 0.07800 19200.00 15000.00 0.00 0.07830 19400.00 15000.00 0.00 0.07916 19600.00 15000.00 0.00 0.07945 19800.00 15000.00 0.00 0.07951 19200.00 15200.00 0.00 0.07997 19400.00 15200.00 0.00 0.08081 19600.00 15200.00 0.00 0.08110 19800.00 15200.00 0.00 0.08119 19200.00 15400.00 0.00 0.08171 19400.00 15400.00 0.00 0.08254 19600.00 15400.00 0.00 0.08282 19800.00 15400.00 0.00 0.08291 19200.00 15600.00 0.00 0.08360 19400.00 15600.00 0.00 0.08443 19600.00 15600.00 0.00 0.08468 19800.00 15600.00 0.00 0.08475 19200.00 15800.00 0.00 0.08540 19400.00 15800.00 0.00 0.08623 19600.00 15800.00 0.00 0.08650 19800.00 15800.00 0.00 0.08657 19200.00 16000.00 0.00 0.08750 19400.00 16000.00 0.00 0.08835 19600.00 16000.00 0.00 0.08861 19800.00 16000.00 0.00 0.08866 19200.00 16200.00 0.00 0.08967 19400.00 16200.00 0.00 0.09049 19600.00 16200.00 0.00 0.09070 19800.00 16200.00 0.00 0.09071 19200.00 16400.00 0.00 0.09182 19400.00 16400.00 0.00 0.09258 19600.00 16400.00 0.00 0.09274 19200.00 16600.00 0.00 0.09393 19400.00 16600.00 0.00 0.09464 19600.00 16600.00 0.00 0.09476 19200.00 16800.00 0.00 0.09608 19400.00 16800.00 0.00 0.09675 19600.00 16800.00 0.00 0.09684 19000.00 17000.00 0.00 0.09688 19200.00 17000.00 0.00 0.09830 19400.00 17000.00 0.00 0.09894 19600.00 17000.00 0.00 0.09895 19000.00 17200.00 0.00 0.09913 19200.00 17200.00 0.00 0.10050 19400.00 17200.00 0.00 0.10108 19000.00 17400.00 0.00 0.10170 19200.00 17400.00 0.00 0.10300 19400.00 17400.00 0.00 0.10350 19000.00 17600.00 0.00 0.10427 19200.00 17600.00 0.00 0.10548 19400.00 17600.00 0.00 0.10588 19000.00 17800.00 0.00 0.10701 19200.00 17800.00 0.00 0.10813 19400.00 17800.00 0.00 0.10843 19000.00 18000.00 0.00 0.10966 19200.00 18000.00 0.00 0.11071 19400.00 18000.00 0.00 0.11089 19000.00 18200.00 0.00 0.11229 19200.00 18200.00 0.00 0.11321 19000.00 18400.00 0.00 0.11488 19200.00 18400.00 0.00 0.11561 18800.00 18600.00 0.00 0.11563 19000.00 18600.00 0.00 0.11735 19200.00 18600.00 0.00 0.11781 18800.00 18800.00 0.00 0.11842 19000.00 18800.00 0.00 0.11967 19200.00 18800.00 0.00 0.11977 18800.00 19000.00 0.00 0.12011 19000.00 19000.00 0.00 0.12082 18800.00 19200.00 0.00 0.12097 19000.00 19200.00 0.00 0.12143 19000.00 19200.00 5.00 0.12148 19000.00 19200.00 10.00 0.12151 19000.00 19200.00 15.00 0.12153 19000.00 19200.00 20.00 0.12153 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19000.00 19200.00 17.30 0.12153 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/539.ctf NX, NY, NZ: 512 512 1 MODE : real