CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/538.mrc Output diagnostic file name Micrographs_0601/538.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/538.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2424.000 8696.000 Mean, RMS : 4542.657 224.6413 TITLE 1: emi2mrc: 538.emi Mon Jan 06 17:10:46 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 35.697, Y 490.974, Z -32.881, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01693 10400.00 10000.00 0.00 -0.01685 13000.00 10000.00 0.00 -0.01667 13200.00 10000.00 0.00 -0.01638 13400.00 10000.00 0.00 -0.01620 13600.00 10000.00 0.00 -0.01590 13800.00 10000.00 0.00 -0.01552 14000.00 10000.00 0.00 -0.01520 14200.00 10000.00 0.00 -0.01480 14400.00 10000.00 0.00 -0.01429 14600.00 10000.00 0.00 -0.01379 14800.00 10000.00 0.00 -0.01327 15000.00 10000.00 0.00 -0.01266 15200.00 10000.00 0.00 -0.01200 15400.00 10000.00 0.00 -0.01125 15600.00 10000.00 0.00 -0.01051 15800.00 10000.00 0.00 -0.00984 16000.00 10000.00 0.00 -0.00920 16200.00 10000.00 0.00 -0.00846 16400.00 10000.00 0.00 -0.00767 16600.00 10000.00 0.00 -0.00696 16800.00 10000.00 0.00 -0.00615 17000.00 10000.00 0.00 -0.00528 17200.00 10000.00 0.00 -0.00431 17400.00 10000.00 0.00 -0.00316 17600.00 10000.00 0.00 -0.00202 17800.00 10000.00 0.00 -0.00087 18000.00 10000.00 0.00 0.00032 18200.00 10000.00 0.00 0.00135 18400.00 10000.00 0.00 0.00240 18600.00 10000.00 0.00 0.00362 18800.00 10000.00 0.00 0.00482 19000.00 10000.00 0.00 0.00595 19200.00 10000.00 0.00 0.00706 19400.00 10000.00 0.00 0.00828 19600.00 10000.00 0.00 0.00949 19800.00 10000.00 0.00 0.01053 20000.00 10000.00 0.00 0.01165 20200.00 10000.00 0.00 0.01284 20400.00 10000.00 0.00 0.01408 20600.00 10000.00 0.00 0.01549 20800.00 10000.00 0.00 0.01694 21000.00 10000.00 0.00 0.01835 21200.00 10000.00 0.00 0.01983 21400.00 10000.00 0.00 0.02148 21600.00 10000.00 0.00 0.02323 21800.00 10000.00 0.00 0.02493 22000.00 10000.00 0.00 0.02660 22200.00 10000.00 0.00 0.02838 22400.00 10000.00 0.00 0.03039 22600.00 10000.00 0.00 0.03245 22800.00 10000.00 0.00 0.03417 23000.00 10000.00 0.00 0.03519 23200.00 10000.00 0.00 0.03567 23400.00 10000.00 0.00 0.03593 23200.00 10200.00 0.00 0.03637 23400.00 10200.00 0.00 0.03664 23600.00 10200.00 0.00 0.03664 23200.00 10400.00 0.00 0.03702 23400.00 10400.00 0.00 0.03729 23600.00 10400.00 0.00 0.03731 23200.00 10600.00 0.00 0.03763 23400.00 10600.00 0.00 0.03792 23600.00 10600.00 0.00 0.03794 23200.00 10800.00 0.00 0.03811 23400.00 10800.00 0.00 0.03839 23200.00 11000.00 0.00 0.03858 23400.00 11000.00 0.00 0.03886 23200.00 11200.00 0.00 0.03913 23400.00 11200.00 0.00 0.03944 23600.00 11200.00 0.00 0.03944 23200.00 11400.00 0.00 0.03973 23400.00 11400.00 0.00 0.04004 23600.00 11400.00 0.00 0.04005 23200.00 11600.00 0.00 0.04035 23400.00 11600.00 0.00 0.04066 23200.00 11800.00 0.00 0.04113 23400.00 11800.00 0.00 0.04143 23200.00 12000.00 0.00 0.04188 23400.00 12000.00 0.00 0.04214 23200.00 12200.00 0.00 0.04254 23400.00 12200.00 0.00 0.04276 23000.00 12400.00 0.00 0.04292 23200.00 12400.00 0.00 0.04333 23400.00 12400.00 0.00 0.04356 23000.00 12600.00 0.00 0.04368 23200.00 12600.00 0.00 0.04408 23400.00 12600.00 0.00 0.04432 23600.00 12600.00 0.00 0.04433 23000.00 12800.00 0.00 0.04435 23200.00 12800.00 0.00 0.04476 23400.00 12800.00 0.00 0.04502 23600.00 12800.00 0.00 0.04503 23000.00 13000.00 0.00 0.04508 23200.00 13000.00 0.00 0.04553 23400.00 13000.00 0.00 0.04581 23000.00 13200.00 0.00 0.04585 23200.00 13200.00 0.00 0.04630 23400.00 13200.00 0.00 0.04658 23000.00 13400.00 0.00 0.04661 23200.00 13400.00 0.00 0.04708 23400.00 13400.00 0.00 0.04735 23000.00 13600.00 0.00 0.04760 23200.00 13600.00 0.00 0.04808 23400.00 13600.00 0.00 0.04832 23000.00 13800.00 0.00 0.04871 23200.00 13800.00 0.00 0.04916 23400.00 13800.00 0.00 0.04934 23000.00 14000.00 0.00 0.04975 23200.00 14000.00 0.00 0.05014 23400.00 14000.00 0.00 0.05024 23000.00 14200.00 0.00 0.05076 23200.00 14200.00 0.00 0.05109 23400.00 14200.00 0.00 0.05113 23000.00 14400.00 0.00 0.05169 23200.00 14400.00 0.00 0.05196 23000.00 14600.00 0.00 0.05262 23200.00 14600.00 0.00 0.05283 23000.00 14800.00 0.00 0.05346 23200.00 14800.00 0.00 0.05366 23000.00 15000.00 0.00 0.05440 23200.00 15000.00 0.00 0.05460 22800.00 15200.00 0.00 0.05467 23000.00 15200.00 0.00 0.05555 23200.00 15200.00 0.00 0.05575 22800.00 15400.00 0.00 0.05586 23000.00 15400.00 0.00 0.05672 23200.00 15400.00 0.00 0.05690 22800.00 15600.00 0.00 0.05704 23000.00 15600.00 0.00 0.05785 23200.00 15600.00 0.00 0.05799 22800.00 15800.00 0.00 0.05822 23000.00 15800.00 0.00 0.05899 23200.00 15800.00 0.00 0.05914 22800.00 16000.00 0.00 0.05957 23000.00 16000.00 0.00 0.06035 23200.00 16000.00 0.00 0.06053 22800.00 16200.00 0.00 0.06098 23000.00 16200.00 0.00 0.06179 23200.00 16200.00 0.00 0.06196 22800.00 16400.00 0.00 0.06239 23000.00 16400.00 0.00 0.06320 23200.00 16400.00 0.00 0.06332 22800.00 16600.00 0.00 0.06375 23000.00 16600.00 0.00 0.06457 23200.00 16600.00 0.00 0.06468 22800.00 16800.00 0.00 0.06516 23000.00 16800.00 0.00 0.06598 23200.00 16800.00 0.00 0.06608 22800.00 17000.00 0.00 0.06658 23000.00 17000.00 0.00 0.06738 23200.00 17000.00 0.00 0.06744 22800.00 17200.00 0.00 0.06788 23000.00 17200.00 0.00 0.06864 23200.00 17200.00 0.00 0.06867 22800.00 17400.00 0.00 0.06923 23000.00 17400.00 0.00 0.06997 23200.00 17400.00 0.00 0.06999 22800.00 17600.00 0.00 0.07066 23000.00 17600.00 0.00 0.07139 23200.00 17600.00 0.00 0.07142 22800.00 17800.00 0.00 0.07224 23000.00 17800.00 0.00 0.07293 22800.00 18000.00 0.00 0.07379 23000.00 18000.00 0.00 0.07445 22800.00 18200.00 0.00 0.07539 23000.00 18200.00 0.00 0.07603 22800.00 18400.00 0.00 0.07700 23000.00 18400.00 0.00 0.07762 22800.00 18600.00 0.00 0.07856 23000.00 18600.00 0.00 0.07915 22800.00 18800.00 0.00 0.08011 23000.00 18800.00 0.00 0.08070 22800.00 19000.00 0.00 0.08163 23000.00 19000.00 0.00 0.08223 22800.00 19200.00 0.00 0.08328 23000.00 19200.00 0.00 0.08387 22800.00 19400.00 0.00 0.08492 23000.00 19400.00 0.00 0.08549 22800.00 19600.00 0.00 0.08675 23000.00 19600.00 0.00 0.08727 22800.00 19800.00 0.00 0.08867 23000.00 19800.00 0.00 0.08919 22800.00 20000.00 0.00 0.09083 23000.00 20000.00 0.00 0.09131 22600.00 20200.00 0.00 0.09135 22800.00 20200.00 0.00 0.09313 23000.00 20200.00 0.00 0.09353 22800.00 20400.00 0.00 0.09525 23000.00 20400.00 0.00 0.09554 22600.00 20600.00 0.00 0.09579 22800.00 20600.00 0.00 0.09746 23000.00 20600.00 0.00 0.09769 22600.00 20800.00 0.00 0.09814 22800.00 20800.00 0.00 0.09980 23000.00 20800.00 0.00 0.09998 22600.00 21000.00 0.00 0.10085 22800.00 21000.00 0.00 0.10250 23000.00 21000.00 0.00 0.10263 22600.00 21200.00 0.00 0.10367 22800.00 21200.00 0.00 0.10528 22600.00 21400.00 0.00 0.10652 22800.00 21400.00 0.00 0.10801 22600.00 21600.00 0.00 0.10944 22800.00 21600.00 0.00 0.11080 22600.00 21800.00 0.00 0.11256 22800.00 21800.00 0.00 0.11374 22600.00 22000.00 0.00 0.11587 22800.00 22000.00 0.00 0.11675 22600.00 22200.00 0.00 0.11885 22800.00 22200.00 0.00 0.11933 22400.00 22400.00 0.00 0.11939 22600.00 22400.00 0.00 0.12170 22600.00 22600.00 0.00 0.12300 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22706.53 22541.18 -45.59 0.12322 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/538.ctf NX, NY, NZ: 512 512 1 MODE : real