CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/535.mrc Output diagnostic file name Micrographs_0601/535.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/535.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2328.000 7986.000 Mean, RMS : 4534.727 218.4500 TITLE 1: emi2mrc: 535.emi Mon Jan 06 18:22:15 2014 TITLE 2: kV 300, Mag 78000x, Def 21660, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 126.553, Y 225.924, Z -46.015, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00740 10200.00 10000.00 0.00 -0.00716 10400.00 10000.00 0.00 -0.00691 10600.00 10000.00 0.00 -0.00650 10800.00 10000.00 0.00 -0.00623 11000.00 10000.00 0.00 -0.00605 11200.00 10000.00 0.00 -0.00570 11400.00 10000.00 0.00 -0.00535 11600.00 10000.00 0.00 -0.00493 11800.00 10000.00 0.00 -0.00437 12000.00 10000.00 0.00 -0.00375 12200.00 10000.00 0.00 -0.00313 12400.00 10000.00 0.00 -0.00261 12600.00 10000.00 0.00 -0.00207 12800.00 10000.00 0.00 -0.00151 13000.00 10000.00 0.00 -0.00097 13200.00 10000.00 0.00 -0.00050 13400.00 10000.00 0.00 0.00000 13600.00 10000.00 0.00 0.00055 13800.00 10000.00 0.00 0.00128 14000.00 10000.00 0.00 0.00224 14200.00 10000.00 0.00 0.00322 14400.00 10000.00 0.00 0.00440 14600.00 10000.00 0.00 0.00571 14800.00 10000.00 0.00 0.00693 15000.00 10000.00 0.00 0.00819 15200.00 10000.00 0.00 0.00957 15400.00 10000.00 0.00 0.01102 15600.00 10000.00 0.00 0.01241 15800.00 10000.00 0.00 0.01381 16000.00 10000.00 0.00 0.01531 16200.00 10000.00 0.00 0.01674 16400.00 10000.00 0.00 0.01816 16600.00 10000.00 0.00 0.01966 16800.00 10000.00 0.00 0.02121 17000.00 10000.00 0.00 0.02293 17200.00 10000.00 0.00 0.02461 17400.00 10000.00 0.00 0.02626 17600.00 10000.00 0.00 0.02806 17800.00 10000.00 0.00 0.02990 18000.00 10000.00 0.00 0.03177 18200.00 10000.00 0.00 0.03372 18400.00 10000.00 0.00 0.03589 18600.00 10000.00 0.00 0.03813 18800.00 10000.00 0.00 0.04026 19000.00 10000.00 0.00 0.04223 19200.00 10000.00 0.00 0.04417 19400.00 10000.00 0.00 0.04601 19600.00 10000.00 0.00 0.04739 19800.00 10000.00 0.00 0.04838 20000.00 10000.00 0.00 0.04903 20200.00 10000.00 0.00 0.04933 20400.00 10000.00 0.00 0.04945 20600.00 10000.00 0.00 0.04955 20000.00 10200.00 0.00 0.04977 20200.00 10200.00 0.00 0.05006 20400.00 10200.00 0.00 0.05022 20600.00 10200.00 0.00 0.05031 20000.00 10400.00 0.00 0.05056 20200.00 10400.00 0.00 0.05087 20400.00 10400.00 0.00 0.05103 20600.00 10400.00 0.00 0.05107 20000.00 10600.00 0.00 0.05149 20200.00 10600.00 0.00 0.05180 20400.00 10600.00 0.00 0.05192 20000.00 10800.00 0.00 0.05248 20200.00 10800.00 0.00 0.05274 20400.00 10800.00 0.00 0.05279 19800.00 11000.00 0.00 0.05299 20000.00 11000.00 0.00 0.05354 20200.00 11000.00 0.00 0.05372 20400.00 11000.00 0.00 0.05375 19800.00 11200.00 0.00 0.05408 20000.00 11200.00 0.00 0.05455 20200.00 11200.00 0.00 0.05471 20400.00 11200.00 0.00 0.05475 19800.00 11400.00 0.00 0.05508 20000.00 11400.00 0.00 0.05552 20200.00 11400.00 0.00 0.05567 20400.00 11400.00 0.00 0.05572 19800.00 11600.00 0.00 0.05604 20000.00 11600.00 0.00 0.05646 20200.00 11600.00 0.00 0.05662 20400.00 11600.00 0.00 0.05666 19800.00 11800.00 0.00 0.05704 20000.00 11800.00 0.00 0.05745 20200.00 11800.00 0.00 0.05762 20400.00 11800.00 0.00 0.05766 19800.00 12000.00 0.00 0.05797 20000.00 12000.00 0.00 0.05839 20200.00 12000.00 0.00 0.05856 20400.00 12000.00 0.00 0.05859 19800.00 12200.00 0.00 0.05892 20000.00 12200.00 0.00 0.05936 20200.00 12200.00 0.00 0.05953 19800.00 12400.00 0.00 0.05994 20000.00 12400.00 0.00 0.06038 20200.00 12400.00 0.00 0.06051 19800.00 12600.00 0.00 0.06104 20000.00 12600.00 0.00 0.06144 20200.00 12600.00 0.00 0.06154 19800.00 12800.00 0.00 0.06231 20000.00 12800.00 0.00 0.06269 20200.00 12800.00 0.00 0.06280 20400.00 12800.00 0.00 0.06283 19800.00 13000.00 0.00 0.06354 20000.00 13000.00 0.00 0.06391 20200.00 13000.00 0.00 0.06402 20400.00 13000.00 0.00 0.06405 19800.00 13200.00 0.00 0.06489 20000.00 13200.00 0.00 0.06519 20200.00 13200.00 0.00 0.06527 20400.00 13200.00 0.00 0.06529 19600.00 13400.00 0.00 0.06540 19800.00 13400.00 0.00 0.06618 20000.00 13400.00 0.00 0.06641 20200.00 13400.00 0.00 0.06650 20400.00 13400.00 0.00 0.06655 19600.00 13600.00 0.00 0.06679 19800.00 13600.00 0.00 0.06753 20000.00 13600.00 0.00 0.06777 20200.00 13600.00 0.00 0.06794 20400.00 13600.00 0.00 0.06806 20600.00 13600.00 0.00 0.06806 19600.00 13800.00 0.00 0.06839 19800.00 13800.00 0.00 0.06911 20000.00 13800.00 0.00 0.06940 20200.00 13800.00 0.00 0.06966 20400.00 13800.00 0.00 0.06979 19600.00 14000.00 0.00 0.06994 19800.00 14000.00 0.00 0.07066 20000.00 14000.00 0.00 0.07103 20200.00 14000.00 0.00 0.07134 20400.00 14000.00 0.00 0.07145 19600.00 14200.00 0.00 0.07162 19800.00 14200.00 0.00 0.07242 20000.00 14200.00 0.00 0.07288 20200.00 14200.00 0.00 0.07322 20400.00 14200.00 0.00 0.07326 19600.00 14400.00 0.00 0.07351 19800.00 14400.00 0.00 0.07438 20000.00 14400.00 0.00 0.07488 20200.00 14400.00 0.00 0.07515 19600.00 14600.00 0.00 0.07547 19800.00 14600.00 0.00 0.07636 20000.00 14600.00 0.00 0.07680 20200.00 14600.00 0.00 0.07697 19600.00 14800.00 0.00 0.07724 19800.00 14800.00 0.00 0.07810 20000.00 14800.00 0.00 0.07848 20200.00 14800.00 0.00 0.07859 19600.00 15000.00 0.00 0.07904 19800.00 15000.00 0.00 0.07985 20000.00 15000.00 0.00 0.08020 20200.00 15000.00 0.00 0.08027 19600.00 15200.00 0.00 0.08082 19800.00 15200.00 0.00 0.08161 20000.00 15200.00 0.00 0.08194 20200.00 15200.00 0.00 0.08195 19600.00 15400.00 0.00 0.08273 19800.00 15400.00 0.00 0.08353 20000.00 15400.00 0.00 0.08381 19600.00 15600.00 0.00 0.08483 19800.00 15600.00 0.00 0.08556 20000.00 15600.00 0.00 0.08571 19600.00 15800.00 0.00 0.08671 19800.00 15800.00 0.00 0.08733 20000.00 15800.00 0.00 0.08734 19400.00 16000.00 0.00 0.08743 19600.00 16000.00 0.00 0.08874 19800.00 16000.00 0.00 0.08928 19400.00 16200.00 0.00 0.08959 19600.00 16200.00 0.00 0.09084 19800.00 16200.00 0.00 0.09137 19400.00 16400.00 0.00 0.09184 19600.00 16400.00 0.00 0.09305 19800.00 16400.00 0.00 0.09352 19400.00 16600.00 0.00 0.09401 19600.00 16600.00 0.00 0.09519 19800.00 16600.00 0.00 0.09561 19400.00 16800.00 0.00 0.09631 19600.00 16800.00 0.00 0.09749 19800.00 16800.00 0.00 0.09786 19400.00 17000.00 0.00 0.09876 19600.00 17000.00 0.00 0.09989 19800.00 17000.00 0.00 0.10019 19400.00 17200.00 0.00 0.10099 19600.00 17200.00 0.00 0.10209 19800.00 17200.00 0.00 0.10233 19400.00 17400.00 0.00 0.10351 19600.00 17400.00 0.00 0.10459 19800.00 17400.00 0.00 0.10478 19400.00 17600.00 0.00 0.10602 19600.00 17600.00 0.00 0.10706 19800.00 17600.00 0.00 0.10712 19400.00 17800.00 0.00 0.10878 19600.00 17800.00 0.00 0.10973 19400.00 18000.00 0.00 0.11170 19600.00 18000.00 0.00 0.11253 19400.00 18200.00 0.00 0.11458 19600.00 18200.00 0.00 0.11530 19200.00 18400.00 0.00 0.11539 19400.00 18400.00 0.00 0.11761 19600.00 18400.00 0.00 0.11820 19200.00 18600.00 0.00 0.11865 19400.00 18600.00 0.00 0.12082 19600.00 18600.00 0.00 0.12113 19200.00 18800.00 0.00 0.12209 19400.00 18800.00 0.00 0.12410 19200.00 19000.00 0.00 0.12507 19400.00 19000.00 0.00 0.12688 19200.00 19200.00 0.00 0.12811 19400.00 19200.00 0.00 0.12938 19200.00 19400.00 0.00 0.12966 19400.00 19400.00 0.00 0.12986 19200.00 19400.00 15.00 0.12987 19200.00 19400.00 20.00 0.12992 19200.00 19400.00 25.00 0.12995 19200.00 19400.00 30.00 0.12997 19200.00 19400.00 35.00 0.12997 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19296.87 19388.57 35.00 0.13038 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/535.ctf NX, NY, NZ: 512 512 1 MODE : real