CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/533.mrc Output diagnostic file name Micrographs_0601/533.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/533.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2378.000 7422.000 Mean, RMS : 4288.129 209.0052 TITLE 1: emi2mrc: 533.emi Mon Jan 06 18:17:06 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.04%, Obj 88.08%, Dif 73.67% TITLE 3: X 37.660, Y 491.734, Z -32.881, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00419 10200.00 10000.00 0.00 -0.00406 10400.00 10000.00 0.00 -0.00381 10600.00 10000.00 0.00 -0.00343 10800.00 10000.00 0.00 -0.00299 11000.00 10000.00 0.00 -0.00248 11200.00 10000.00 0.00 -0.00216 11400.00 10000.00 0.00 -0.00192 11600.00 10000.00 0.00 -0.00143 11800.00 10000.00 0.00 -0.00086 12000.00 10000.00 0.00 -0.00021 12200.00 10000.00 0.00 0.00038 12400.00 10000.00 0.00 0.00089 12600.00 10000.00 0.00 0.00163 12800.00 10000.00 0.00 0.00247 13000.00 10000.00 0.00 0.00319 13200.00 10000.00 0.00 0.00377 13400.00 10000.00 0.00 0.00430 13600.00 10000.00 0.00 0.00495 13800.00 10000.00 0.00 0.00584 14000.00 10000.00 0.00 0.00674 14200.00 10000.00 0.00 0.00764 14400.00 10000.00 0.00 0.00851 14600.00 10000.00 0.00 0.00934 14800.00 10000.00 0.00 0.01025 15000.00 10000.00 0.00 0.01107 15200.00 10000.00 0.00 0.01198 15400.00 10000.00 0.00 0.01302 15600.00 10000.00 0.00 0.01422 15800.00 10000.00 0.00 0.01547 16000.00 10000.00 0.00 0.01652 16200.00 10000.00 0.00 0.01769 16400.00 10000.00 0.00 0.01903 16600.00 10000.00 0.00 0.02055 16800.00 10000.00 0.00 0.02210 17000.00 10000.00 0.00 0.02360 17200.00 10000.00 0.00 0.02518 17400.00 10000.00 0.00 0.02673 17600.00 10000.00 0.00 0.02818 17800.00 10000.00 0.00 0.02951 18000.00 10000.00 0.00 0.03098 18200.00 10000.00 0.00 0.03243 18400.00 10000.00 0.00 0.03379 18600.00 10000.00 0.00 0.03528 18800.00 10000.00 0.00 0.03669 19000.00 10000.00 0.00 0.03828 19200.00 10000.00 0.00 0.04010 19400.00 10000.00 0.00 0.04169 19600.00 10000.00 0.00 0.04314 19800.00 10000.00 0.00 0.04436 20000.00 10000.00 0.00 0.04520 20200.00 10000.00 0.00 0.04567 20400.00 10000.00 0.00 0.04606 20600.00 10000.00 0.00 0.04655 20800.00 10000.00 0.00 0.04686 21000.00 10000.00 0.00 0.04701 21200.00 10000.00 0.00 0.04709 21400.00 10000.00 0.00 0.04716 22800.00 10000.00 0.00 0.04729 23000.00 10000.00 0.00 0.04751 23200.00 10000.00 0.00 0.04771 23400.00 10000.00 0.00 0.04780 21400.00 10200.00 0.00 0.04781 22800.00 10200.00 0.00 0.04789 23000.00 10200.00 0.00 0.04806 23200.00 10200.00 0.00 0.04821 23400.00 10200.00 0.00 0.04825 21200.00 10400.00 0.00 0.04837 21400.00 10400.00 0.00 0.04846 23000.00 10400.00 0.00 0.04852 23200.00 10400.00 0.00 0.04865 23400.00 10400.00 0.00 0.04868 20800.00 10600.00 0.00 0.04877 21000.00 10600.00 0.00 0.04892 21200.00 10600.00 0.00 0.04905 21400.00 10600.00 0.00 0.04910 23000.00 10600.00 0.00 0.04914 23200.00 10600.00 0.00 0.04931 23400.00 10600.00 0.00 0.04935 20800.00 10800.00 0.00 0.04954 21000.00 10800.00 0.00 0.04970 21200.00 10800.00 0.00 0.04981 21400.00 10800.00 0.00 0.04982 23000.00 10800.00 0.00 0.04996 23200.00 10800.00 0.00 0.05012 20800.00 11000.00 0.00 0.05030 21000.00 11000.00 0.00 0.05045 21200.00 11000.00 0.00 0.05055 21400.00 11000.00 0.00 0.05057 23000.00 11000.00 0.00 0.05069 23200.00 11000.00 0.00 0.05079 20600.00 11200.00 0.00 0.05082 20800.00 11200.00 0.00 0.05103 21000.00 11200.00 0.00 0.05121 21200.00 11200.00 0.00 0.05135 21400.00 11200.00 0.00 0.05139 20600.00 11400.00 0.00 0.05166 20800.00 11400.00 0.00 0.05190 21000.00 11400.00 0.00 0.05213 21200.00 11400.00 0.00 0.05227 20600.00 11600.00 0.00 0.05263 20800.00 11600.00 0.00 0.05289 21000.00 11600.00 0.00 0.05312 21200.00 11600.00 0.00 0.05319 20400.00 11800.00 0.00 0.05328 20600.00 11800.00 0.00 0.05379 20800.00 11800.00 0.00 0.05402 21000.00 11800.00 0.00 0.05419 20400.00 12000.00 0.00 0.05446 20600.00 12000.00 0.00 0.05494 20800.00 12000.00 0.00 0.05513 21000.00 12000.00 0.00 0.05528 20400.00 12200.00 0.00 0.05574 20600.00 12200.00 0.00 0.05619 20800.00 12200.00 0.00 0.05638 21000.00 12200.00 0.00 0.05653 20200.00 12400.00 0.00 0.05662 20400.00 12400.00 0.00 0.05708 20600.00 12400.00 0.00 0.05748 20800.00 12400.00 0.00 0.05765 21000.00 12400.00 0.00 0.05776 20200.00 12600.00 0.00 0.05777 20400.00 12600.00 0.00 0.05819 20600.00 12600.00 0.00 0.05854 20800.00 12600.00 0.00 0.05867 21000.00 12600.00 0.00 0.05876 20200.00 12800.00 0.00 0.05893 20400.00 12800.00 0.00 0.05928 20600.00 12800.00 0.00 0.05959 20800.00 12800.00 0.00 0.05972 21000.00 12800.00 0.00 0.05980 20200.00 13000.00 0.00 0.05994 20400.00 13000.00 0.00 0.06022 20600.00 13000.00 0.00 0.06051 20800.00 13000.00 0.00 0.06064 21000.00 13000.00 0.00 0.06072 20200.00 13200.00 0.00 0.06087 20400.00 13200.00 0.00 0.06112 20600.00 13200.00 0.00 0.06144 20800.00 13200.00 0.00 0.06159 21000.00 13200.00 0.00 0.06164 20200.00 13400.00 0.00 0.06193 20400.00 13400.00 0.00 0.06220 20600.00 13400.00 0.00 0.06254 20800.00 13400.00 0.00 0.06264 21000.00 13400.00 0.00 0.06265 20000.00 13600.00 0.00 0.06271 20200.00 13600.00 0.00 0.06303 20400.00 13600.00 0.00 0.06331 20600.00 13600.00 0.00 0.06363 20800.00 13600.00 0.00 0.06367 21000.00 13600.00 0.00 0.06369 20000.00 13800.00 0.00 0.06383 20200.00 13800.00 0.00 0.06418 20400.00 13800.00 0.00 0.06445 20600.00 13800.00 0.00 0.06473 20800.00 13800.00 0.00 0.06479 21000.00 13800.00 0.00 0.06488 20000.00 14000.00 0.00 0.06513 20200.00 14000.00 0.00 0.06548 20400.00 14000.00 0.00 0.06575 20600.00 14000.00 0.00 0.06602 20800.00 14000.00 0.00 0.06613 21000.00 14000.00 0.00 0.06629 20000.00 14200.00 0.00 0.06657 20200.00 14200.00 0.00 0.06692 20400.00 14200.00 0.00 0.06717 20600.00 14200.00 0.00 0.06746 20800.00 14200.00 0.00 0.06761 21000.00 14200.00 0.00 0.06775 20000.00 14400.00 0.00 0.06793 20200.00 14400.00 0.00 0.06824 20400.00 14400.00 0.00 0.06846 20600.00 14400.00 0.00 0.06875 20800.00 14400.00 0.00 0.06889 21000.00 14400.00 0.00 0.06897 20000.00 14600.00 0.00 0.06930 20200.00 14600.00 0.00 0.06955 20400.00 14600.00 0.00 0.06975 20600.00 14600.00 0.00 0.07003 20800.00 14600.00 0.00 0.07016 21000.00 14600.00 0.00 0.07020 20000.00 14800.00 0.00 0.07064 20200.00 14800.00 0.00 0.07085 20400.00 14800.00 0.00 0.07106 20600.00 14800.00 0.00 0.07137 20800.00 14800.00 0.00 0.07150 21000.00 14800.00 0.00 0.07152 20000.00 15000.00 0.00 0.07199 20200.00 15000.00 0.00 0.07222 20400.00 15000.00 0.00 0.07248 20600.00 15000.00 0.00 0.07281 20800.00 15000.00 0.00 0.07296 21000.00 15000.00 0.00 0.07299 20000.00 15200.00 0.00 0.07350 20200.00 15200.00 0.00 0.07379 20400.00 15200.00 0.00 0.07408 20600.00 15200.00 0.00 0.07444 20800.00 15200.00 0.00 0.07460 21000.00 15200.00 0.00 0.07465 20000.00 15400.00 0.00 0.07534 20200.00 15400.00 0.00 0.07563 20400.00 15400.00 0.00 0.07590 20600.00 15400.00 0.00 0.07624 20800.00 15400.00 0.00 0.07638 21000.00 15400.00 0.00 0.07640 20000.00 15600.00 0.00 0.07714 20200.00 15600.00 0.00 0.07735 20400.00 15600.00 0.00 0.07757 20600.00 15600.00 0.00 0.07791 20800.00 15600.00 0.00 0.07803 20000.00 15800.00 0.00 0.07847 20200.00 15800.00 0.00 0.07864 20400.00 15800.00 0.00 0.07891 20600.00 15800.00 0.00 0.07930 20800.00 15800.00 0.00 0.07938 20000.00 16000.00 0.00 0.07983 20200.00 16000.00 0.00 0.08005 20400.00 16000.00 0.00 0.08039 20600.00 16000.00 0.00 0.08076 20000.00 16200.00 0.00 0.08139 20200.00 16200.00 0.00 0.08163 20400.00 16200.00 0.00 0.08196 20600.00 16200.00 0.00 0.08221 19800.00 16400.00 0.00 0.08231 20000.00 16400.00 0.00 0.08306 20200.00 16400.00 0.00 0.08326 20400.00 16400.00 0.00 0.08350 20600.00 16400.00 0.00 0.08362 19800.00 16600.00 0.00 0.08391 20000.00 16600.00 0.00 0.08463 20200.00 16600.00 0.00 0.08478 20400.00 16600.00 0.00 0.08496 20600.00 16600.00 0.00 0.08502 19800.00 16800.00 0.00 0.08556 20000.00 16800.00 0.00 0.08627 20200.00 16800.00 0.00 0.08640 20400.00 16800.00 0.00 0.08654 20600.00 16800.00 0.00 0.08662 19800.00 17000.00 0.00 0.08727 20000.00 17000.00 0.00 0.08796 20200.00 17000.00 0.00 0.08806 20400.00 17000.00 0.00 0.08820 20600.00 17000.00 0.00 0.08829 19800.00 17200.00 0.00 0.08899 20000.00 17200.00 0.00 0.08964 20200.00 17200.00 0.00 0.08970 20400.00 17200.00 0.00 0.08980 20600.00 17200.00 0.00 0.08986 19800.00 17400.00 0.00 0.09086 20000.00 17400.00 0.00 0.09144 20400.00 17400.00 0.00 0.09151 20600.00 17400.00 0.00 0.09153 19800.00 17600.00 0.00 0.09260 20000.00 17600.00 0.00 0.09312 20400.00 17600.00 0.00 0.09314 20600.00 17600.00 0.00 0.09315 19800.00 17800.00 0.00 0.09467 20000.00 17800.00 0.00 0.09512 19600.00 18000.00 0.00 0.09528 19800.00 18000.00 0.00 0.09677 20000.00 18000.00 0.00 0.09711 19600.00 18200.00 0.00 0.09752 19800.00 18200.00 0.00 0.09891 20000.00 18200.00 0.00 0.09917 19600.00 18400.00 0.00 0.09990 19800.00 18400.00 0.00 0.10121 20000.00 18400.00 0.00 0.10140 19600.00 18600.00 0.00 0.10216 19800.00 18600.00 0.00 0.10339 20000.00 18600.00 0.00 0.10349 19600.00 18800.00 0.00 0.10448 19800.00 18800.00 0.00 0.10560 19600.00 19000.00 0.00 0.10671 19800.00 19000.00 0.00 0.10774 19600.00 19200.00 0.00 0.10931 19800.00 19200.00 0.00 0.11019 19400.00 19400.00 0.00 0.11025 19600.00 19400.00 0.00 0.11170 19800.00 19400.00 0.00 0.11238 19400.00 19600.00 0.00 0.11239 19600.00 19600.00 0.00 0.11373 19800.00 19600.00 0.00 0.11405 19600.00 19800.00 0.00 0.11463 19600.00 19800.00 5.00 0.11469 19600.00 19800.00 10.00 0.11475 19600.00 19800.00 15.00 0.11478 19600.00 19800.00 20.00 0.11481 19600.00 19800.00 25.00 0.11482 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19656.48 19806.79 27.76 0.11489 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/533.ctf NX, NY, NZ: 512 512 1 MODE : real