CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/532.mrc Output diagnostic file name Micrographs_0601/532.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/532.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2168.000 7870.000 Mean, RMS : 4447.690 215.1048 TITLE 1: emi2mrc: 532.emi Mon Jan 06 18:16:02 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.04%, Obj 88.10%, Dif 73.67% TITLE 3: X 36.092, Y 493.971, Z -32.881, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04388 10200.00 10000.00 0.00 0.04659 10400.00 10000.00 0.00 0.04912 10600.00 10000.00 0.00 0.05207 10800.00 10000.00 0.00 0.05496 11000.00 10000.00 0.00 0.05790 11200.00 10000.00 0.00 0.06068 11400.00 10000.00 0.00 0.06363 11600.00 10000.00 0.00 0.06679 11800.00 10000.00 0.00 0.06966 12000.00 10000.00 0.00 0.07276 12200.00 10000.00 0.00 0.07565 12400.00 10000.00 0.00 0.07859 12600.00 10000.00 0.00 0.08154 12800.00 10000.00 0.00 0.08438 13000.00 10000.00 0.00 0.08698 13200.00 10000.00 0.00 0.08886 13400.00 10000.00 0.00 0.09005 13600.00 10000.00 0.00 0.09059 13800.00 10000.00 0.00 0.09078 13200.00 10200.00 0.00 0.09200 13400.00 10200.00 0.00 0.09311 13600.00 10200.00 0.00 0.09358 13800.00 10200.00 0.00 0.09369 13200.00 10400.00 0.00 0.09535 13400.00 10400.00 0.00 0.09638 13600.00 10400.00 0.00 0.09677 13800.00 10400.00 0.00 0.09678 13000.00 10600.00 0.00 0.09726 13200.00 10600.00 0.00 0.09898 13400.00 10600.00 0.00 0.09995 13600.00 10600.00 0.00 0.10026 13000.00 10800.00 0.00 0.10087 13200.00 10800.00 0.00 0.10254 13400.00 10800.00 0.00 0.10345 13600.00 10800.00 0.00 0.10367 13000.00 11000.00 0.00 0.10474 13200.00 11000.00 0.00 0.10632 13400.00 11000.00 0.00 0.10716 13600.00 11000.00 0.00 0.10722 13000.00 11200.00 0.00 0.10836 13200.00 11200.00 0.00 0.10985 13400.00 11200.00 0.00 0.11055 13000.00 11400.00 0.00 0.11227 13200.00 11400.00 0.00 0.11365 13400.00 11400.00 0.00 0.11424 13000.00 11600.00 0.00 0.11620 13200.00 11600.00 0.00 0.11750 13400.00 11600.00 0.00 0.11799 13000.00 11800.00 0.00 0.12038 13200.00 11800.00 0.00 0.12158 13400.00 11800.00 0.00 0.12192 12800.00 12000.00 0.00 0.12221 13000.00 12000.00 0.00 0.12467 13200.00 12000.00 0.00 0.12573 13400.00 12000.00 0.00 0.12586 12800.00 12200.00 0.00 0.12633 13000.00 12200.00 0.00 0.12872 13200.00 12200.00 0.00 0.12960 12800.00 12400.00 0.00 0.13066 13000.00 12400.00 0.00 0.13297 13200.00 12400.00 0.00 0.13360 12800.00 12600.00 0.00 0.13449 13000.00 12600.00 0.00 0.13680 13200.00 12600.00 0.00 0.13709 12800.00 12800.00 0.00 0.13893 13000.00 12800.00 0.00 0.14078 12800.00 13000.00 0.00 0.14166 13000.00 13000.00 0.00 0.14231 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12928.00 13010.51 28.37 0.14258 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/532.ctf NX, NY, NZ: 512 512 1 MODE : real