CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/531.mrc Output diagnostic file name Micrographs_0601/531.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/531.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2312.000 8508.000 Mean, RMS : 4472.015 212.4206 TITLE 1: emi2mrc: 531.emi Mon Jan 06 18:14:57 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.04%, Obj 88.10%, Dif 73.67% TITLE 3: X 30.473, Y 497.083, Z -32.881, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00479 10200.00 10000.00 0.00 0.00557 10400.00 10000.00 0.00 0.00620 10600.00 10000.00 0.00 0.00717 10800.00 10000.00 0.00 0.00834 11000.00 10000.00 0.00 0.00979 11200.00 10000.00 0.00 0.01120 11400.00 10000.00 0.00 0.01248 11600.00 10000.00 0.00 0.01393 11800.00 10000.00 0.00 0.01545 12000.00 10000.00 0.00 0.01704 12200.00 10000.00 0.00 0.01870 12400.00 10000.00 0.00 0.02055 12600.00 10000.00 0.00 0.02240 12800.00 10000.00 0.00 0.02431 13000.00 10000.00 0.00 0.02624 13200.00 10000.00 0.00 0.02805 13400.00 10000.00 0.00 0.03026 13600.00 10000.00 0.00 0.03254 13800.00 10000.00 0.00 0.03463 14000.00 10000.00 0.00 0.03677 14200.00 10000.00 0.00 0.03911 14400.00 10000.00 0.00 0.04154 14600.00 10000.00 0.00 0.04414 14800.00 10000.00 0.00 0.04700 15000.00 10000.00 0.00 0.04981 15200.00 10000.00 0.00 0.05269 15400.00 10000.00 0.00 0.05566 15600.00 10000.00 0.00 0.05896 15800.00 10000.00 0.00 0.06251 16000.00 10000.00 0.00 0.06593 16200.00 10000.00 0.00 0.06890 16400.00 10000.00 0.00 0.07066 16600.00 10000.00 0.00 0.07100 16400.00 10200.00 0.00 0.07178 16600.00 10200.00 0.00 0.07208 16400.00 10400.00 0.00 0.07309 16600.00 10400.00 0.00 0.07338 16400.00 10600.00 0.00 0.07453 16600.00 10600.00 0.00 0.07478 16400.00 10800.00 0.00 0.07594 16600.00 10800.00 0.00 0.07612 16400.00 11000.00 0.00 0.07751 16600.00 11000.00 0.00 0.07764 16400.00 11200.00 0.00 0.07898 16600.00 11200.00 0.00 0.07904 16400.00 11400.00 0.00 0.08029 16400.00 11600.00 0.00 0.08147 16200.00 11800.00 0.00 0.08164 16400.00 11800.00 0.00 0.08294 16200.00 12000.00 0.00 0.08345 16400.00 12000.00 0.00 0.08469 16200.00 12200.00 0.00 0.08521 16400.00 12200.00 0.00 0.08639 16200.00 12400.00 0.00 0.08707 16400.00 12400.00 0.00 0.08822 16200.00 12600.00 0.00 0.08905 16400.00 12600.00 0.00 0.09018 16200.00 12800.00 0.00 0.09106 16400.00 12800.00 0.00 0.09216 16200.00 13000.00 0.00 0.09294 16400.00 13000.00 0.00 0.09393 16200.00 13200.00 0.00 0.09483 16400.00 13200.00 0.00 0.09570 16200.00 13400.00 0.00 0.09668 16400.00 13400.00 0.00 0.09748 16200.00 13600.00 0.00 0.09867 16400.00 13600.00 0.00 0.09949 16200.00 13800.00 0.00 0.10076 16400.00 13800.00 0.00 0.10155 16200.00 14000.00 0.00 0.10275 16400.00 14000.00 0.00 0.10341 16200.00 14200.00 0.00 0.10482 16400.00 14200.00 0.00 0.10540 16200.00 14400.00 0.00 0.10680 16400.00 14400.00 0.00 0.10739 16200.00 14600.00 0.00 0.10896 16400.00 14600.00 0.00 0.10951 16200.00 14800.00 0.00 0.11095 16400.00 14800.00 0.00 0.11143 16200.00 15000.00 0.00 0.11295 16400.00 15000.00 0.00 0.11329 16200.00 15200.00 0.00 0.11487 16400.00 15200.00 0.00 0.11509 16200.00 15400.00 0.00 0.11692 16400.00 15400.00 0.00 0.11700 16200.00 15600.00 0.00 0.11914 16200.00 15800.00 0.00 0.12064 16200.00 16000.00 0.00 0.12239 16000.00 16200.00 0.00 0.12241 16200.00 16200.00 0.00 0.12357 16200.00 16400.00 0.00 0.12378 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16132.42 16418.44 6.83 0.12391 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/531.ctf NX, NY, NZ: 512 512 1 MODE : real