CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/530.mrc Output diagnostic file name Micrographs_0601/530.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/530.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2520.000 7572.000 Mean, RMS : 4522.888 219.7252 TITLE 1: emi2mrc: 530.emi Mon Jan 06 18:13:58 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 32.248, Y 494.991, Z -32.881, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02364 12600.00 10000.00 0.00 -0.02358 12800.00 10000.00 0.00 -0.02322 13000.00 10000.00 0.00 -0.02273 13200.00 10000.00 0.00 -0.02230 13400.00 10000.00 0.00 -0.02217 13600.00 10000.00 0.00 -0.02200 13800.00 10000.00 0.00 -0.02194 14000.00 10000.00 0.00 -0.02191 14200.00 10000.00 0.00 -0.02184 14400.00 10000.00 0.00 -0.02166 14600.00 10000.00 0.00 -0.02131 14800.00 10000.00 0.00 -0.02100 15000.00 10000.00 0.00 -0.02055 15200.00 10000.00 0.00 -0.02009 15400.00 10000.00 0.00 -0.01969 15600.00 10000.00 0.00 -0.01917 15800.00 10000.00 0.00 -0.01865 16000.00 10000.00 0.00 -0.01809 16200.00 10000.00 0.00 -0.01747 16400.00 10000.00 0.00 -0.01677 16600.00 10000.00 0.00 -0.01597 16800.00 10000.00 0.00 -0.01508 17000.00 10000.00 0.00 -0.01415 17200.00 10000.00 0.00 -0.01332 17400.00 10000.00 0.00 -0.01240 17600.00 10000.00 0.00 -0.01138 17800.00 10000.00 0.00 -0.01047 18000.00 10000.00 0.00 -0.00939 18200.00 10000.00 0.00 -0.00840 18400.00 10000.00 0.00 -0.00763 18600.00 10000.00 0.00 -0.00665 18800.00 10000.00 0.00 -0.00562 19000.00 10000.00 0.00 -0.00463 19200.00 10000.00 0.00 -0.00363 19400.00 10000.00 0.00 -0.00261 19600.00 10000.00 0.00 -0.00163 19800.00 10000.00 0.00 -0.00063 20000.00 10000.00 0.00 0.00050 20200.00 10000.00 0.00 0.00150 20400.00 10000.00 0.00 0.00243 20600.00 10000.00 0.00 0.00351 20800.00 10000.00 0.00 0.00459 21000.00 10000.00 0.00 0.00569 21200.00 10000.00 0.00 0.00678 21400.00 10000.00 0.00 0.00785 21600.00 10000.00 0.00 0.00900 21800.00 10000.00 0.00 0.01019 22000.00 10000.00 0.00 0.01139 22200.00 10000.00 0.00 0.01265 22400.00 10000.00 0.00 0.01407 22600.00 10000.00 0.00 0.01559 22800.00 10000.00 0.00 0.01723 23000.00 10000.00 0.00 0.01883 23200.00 10000.00 0.00 0.02042 23400.00 10000.00 0.00 0.02221 23600.00 10000.00 0.00 0.02401 23800.00 10000.00 0.00 0.02572 24000.00 10000.00 0.00 0.02704 24200.00 10000.00 0.00 0.02761 24400.00 10000.00 0.00 0.02771 24200.00 10200.00 0.00 0.02789 24400.00 10200.00 0.00 0.02804 24600.00 10200.00 0.00 0.02804 24200.00 10400.00 0.00 0.02816 24400.00 10400.00 0.00 0.02834 24600.00 10400.00 0.00 0.02834 24200.00 10600.00 0.00 0.02843 24400.00 10600.00 0.00 0.02865 24600.00 10600.00 0.00 0.02866 24200.00 10800.00 0.00 0.02871 24400.00 10800.00 0.00 0.02896 24600.00 10800.00 0.00 0.02897 24200.00 11000.00 0.00 0.02911 24400.00 11000.00 0.00 0.02939 24600.00 11000.00 0.00 0.02939 24200.00 11200.00 0.00 0.02952 24400.00 11200.00 0.00 0.02982 24600.00 11200.00 0.00 0.02985 24200.00 11400.00 0.00 0.03005 24400.00 11400.00 0.00 0.03038 24600.00 11400.00 0.00 0.03040 24200.00 11600.00 0.00 0.03066 24400.00 11600.00 0.00 0.03097 24200.00 11800.00 0.00 0.03123 24400.00 11800.00 0.00 0.03149 24200.00 12000.00 0.00 0.03181 24400.00 12000.00 0.00 0.03204 24200.00 12200.00 0.00 0.03246 24400.00 12200.00 0.00 0.03266 24200.00 12400.00 0.00 0.03310 24400.00 12400.00 0.00 0.03327 24200.00 12600.00 0.00 0.03368 24400.00 12600.00 0.00 0.03381 24200.00 12800.00 0.00 0.03441 24400.00 12800.00 0.00 0.03449 24200.00 13000.00 0.00 0.03510 24400.00 13000.00 0.00 0.03513 24200.00 13200.00 0.00 0.03573 24400.00 13200.00 0.00 0.03579 24600.00 13200.00 0.00 0.03583 24800.00 13200.00 0.00 0.03588 24200.00 13400.00 0.00 0.03649 24400.00 13400.00 0.00 0.03666 24600.00 13400.00 0.00 0.03681 24800.00 13400.00 0.00 0.03687 24200.00 13600.00 0.00 0.03742 24400.00 13600.00 0.00 0.03769 24600.00 13600.00 0.00 0.03785 24800.00 13600.00 0.00 0.03786 24200.00 13800.00 0.00 0.03850 24400.00 13800.00 0.00 0.03880 24600.00 13800.00 0.00 0.03891 24200.00 14000.00 0.00 0.03943 24400.00 14000.00 0.00 0.03971 24600.00 14000.00 0.00 0.03978 24200.00 14200.00 0.00 0.04025 24400.00 14200.00 0.00 0.04053 24600.00 14200.00 0.00 0.04060 24200.00 14400.00 0.00 0.04109 24400.00 14400.00 0.00 0.04138 24600.00 14400.00 0.00 0.04143 24200.00 14600.00 0.00 0.04191 24400.00 14600.00 0.00 0.04217 24200.00 14800.00 0.00 0.04276 24400.00 14800.00 0.00 0.04298 24200.00 15000.00 0.00 0.04370 24400.00 15000.00 0.00 0.04388 24200.00 15200.00 0.00 0.04467 24400.00 15200.00 0.00 0.04484 24000.00 15400.00 0.00 0.04484 24200.00 15400.00 0.00 0.04568 24400.00 15400.00 0.00 0.04584 24200.00 15600.00 0.00 0.04665 24400.00 15600.00 0.00 0.04680 24000.00 15800.00 0.00 0.04687 24200.00 15800.00 0.00 0.04767 24400.00 15800.00 0.00 0.04781 24000.00 16000.00 0.00 0.04796 24200.00 16000.00 0.00 0.04871 24400.00 16000.00 0.00 0.04882 24000.00 16200.00 0.00 0.04887 24200.00 16200.00 0.00 0.04956 24400.00 16200.00 0.00 0.04967 24000.00 16400.00 0.00 0.04990 24200.00 16400.00 0.00 0.05059 24400.00 16400.00 0.00 0.05073 24000.00 16600.00 0.00 0.05090 24200.00 16600.00 0.00 0.05160 24400.00 16600.00 0.00 0.05178 24000.00 16800.00 0.00 0.05185 24200.00 16800.00 0.00 0.05260 24400.00 16800.00 0.00 0.05280 24000.00 17000.00 0.00 0.05308 24200.00 17000.00 0.00 0.05385 24400.00 17000.00 0.00 0.05400 24000.00 17200.00 0.00 0.05432 24200.00 17200.00 0.00 0.05503 24400.00 17200.00 0.00 0.05509 24000.00 17400.00 0.00 0.05553 24200.00 17400.00 0.00 0.05619 24400.00 17400.00 0.00 0.05620 24000.00 17600.00 0.00 0.05671 24200.00 17600.00 0.00 0.05738 24400.00 17600.00 0.00 0.05739 24000.00 17800.00 0.00 0.05797 24200.00 17800.00 0.00 0.05865 24400.00 17800.00 0.00 0.05866 24000.00 18000.00 0.00 0.05934 24200.00 18000.00 0.00 0.06000 24000.00 18200.00 0.00 0.06083 24200.00 18200.00 0.00 0.06144 24000.00 18400.00 0.00 0.06224 24200.00 18400.00 0.00 0.06281 24000.00 18600.00 0.00 0.06347 24200.00 18600.00 0.00 0.06403 24000.00 18800.00 0.00 0.06475 24200.00 18800.00 0.00 0.06535 24000.00 19000.00 0.00 0.06608 24200.00 19000.00 0.00 0.06670 24000.00 19200.00 0.00 0.06759 24200.00 19200.00 0.00 0.06818 24000.00 19400.00 0.00 0.06917 24200.00 19400.00 0.00 0.06973 24000.00 19600.00 0.00 0.07069 24200.00 19600.00 0.00 0.07120 24000.00 19800.00 0.00 0.07217 24200.00 19800.00 0.00 0.07262 24000.00 20000.00 0.00 0.07357 24200.00 20000.00 0.00 0.07396 24000.00 20200.00 0.00 0.07494 24200.00 20200.00 0.00 0.07529 24000.00 20400.00 0.00 0.07647 24200.00 20400.00 0.00 0.07678 24000.00 20600.00 0.00 0.07809 24200.00 20600.00 0.00 0.07833 24000.00 20800.00 0.00 0.07965 24200.00 20800.00 0.00 0.07979 23800.00 21000.00 0.00 0.07987 24000.00 21000.00 0.00 0.08134 24200.00 21000.00 0.00 0.08145 23800.00 21200.00 0.00 0.08155 24000.00 21200.00 0.00 0.08304 24200.00 21200.00 0.00 0.08314 23800.00 21400.00 0.00 0.08339 24000.00 21400.00 0.00 0.08489 24200.00 21400.00 0.00 0.08495 23800.00 21600.00 0.00 0.08516 24000.00 21600.00 0.00 0.08661 23800.00 21800.00 0.00 0.08668 24000.00 21800.00 0.00 0.08808 23800.00 22000.00 0.00 0.08842 24000.00 22000.00 0.00 0.08977 23800.00 22200.00 0.00 0.09020 24000.00 22200.00 0.00 0.09145 23800.00 22400.00 0.00 0.09206 24000.00 22400.00 0.00 0.09324 23800.00 22600.00 0.00 0.09390 24000.00 22600.00 0.00 0.09501 23800.00 22800.00 0.00 0.09570 24000.00 22800.00 0.00 0.09674 23800.00 23000.00 0.00 0.09736 24000.00 23000.00 0.00 0.09831 23800.00 23200.00 0.00 0.09915 24000.00 23200.00 0.00 0.09998 23800.00 23400.00 0.00 0.10100 24000.00 23400.00 0.00 0.10162 23800.00 23600.00 0.00 0.10256 24000.00 23600.00 0.00 0.10291 23800.00 23800.00 0.00 0.10426 23800.00 24000.00 0.00 0.10464 23800.00 24000.00 5.00 0.10470 23800.00 24000.00 10.00 0.10476 23800.00 24000.00 15.00 0.10480 23800.00 24000.00 20.00 0.10484 23800.00 24000.00 25.00 0.10486 23800.00 24000.00 30.00 0.10487 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23831.15 23933.62 30.34 0.10499 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/530.ctf NX, NY, NZ: 512 512 1 MODE : real