CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/529.mrc Output diagnostic file name Micrographs_0601/529.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/529.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2162.000 7976.000 Mean, RMS : 4519.485 229.2332 TITLE 1: emi2mrc: 529.emi Mon Jan 06 18:12:51 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 33.939, Y 492.582, Z -32.881, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02415 10800.00 10000.00 0.00 -0.02414 11000.00 10000.00 0.00 -0.02395 11200.00 10000.00 0.00 -0.02379 11400.00 10000.00 0.00 -0.02360 11600.00 10000.00 0.00 -0.02334 11800.00 10000.00 0.00 -0.02306 12000.00 10000.00 0.00 -0.02287 12200.00 10000.00 0.00 -0.02255 12400.00 10000.00 0.00 -0.02223 12600.00 10000.00 0.00 -0.02185 12800.00 10000.00 0.00 -0.02134 13000.00 10000.00 0.00 -0.02090 13200.00 10000.00 0.00 -0.02046 13400.00 10000.00 0.00 -0.02003 13600.00 10000.00 0.00 -0.01955 13800.00 10000.00 0.00 -0.01891 14000.00 10000.00 0.00 -0.01832 14200.00 10000.00 0.00 -0.01783 14400.00 10000.00 0.00 -0.01725 14600.00 10000.00 0.00 -0.01666 14800.00 10000.00 0.00 -0.01600 15000.00 10000.00 0.00 -0.01528 15200.00 10000.00 0.00 -0.01470 15400.00 10000.00 0.00 -0.01403 15600.00 10000.00 0.00 -0.01318 15800.00 10000.00 0.00 -0.01220 16000.00 10000.00 0.00 -0.01120 16200.00 10000.00 0.00 -0.01023 16400.00 10000.00 0.00 -0.00921 16600.00 10000.00 0.00 -0.00824 16800.00 10000.00 0.00 -0.00722 17000.00 10000.00 0.00 -0.00607 17200.00 10000.00 0.00 -0.00494 17400.00 10000.00 0.00 -0.00379 17600.00 10000.00 0.00 -0.00254 17800.00 10000.00 0.00 -0.00128 18000.00 10000.00 0.00 -0.00019 18200.00 10000.00 0.00 0.00103 18400.00 10000.00 0.00 0.00232 18600.00 10000.00 0.00 0.00343 18800.00 10000.00 0.00 0.00467 19000.00 10000.00 0.00 0.00584 19200.00 10000.00 0.00 0.00703 19400.00 10000.00 0.00 0.00853 19600.00 10000.00 0.00 0.01009 19800.00 10000.00 0.00 0.01167 20000.00 10000.00 0.00 0.01328 20200.00 10000.00 0.00 0.01488 20400.00 10000.00 0.00 0.01658 20600.00 10000.00 0.00 0.01840 20800.00 10000.00 0.00 0.02028 21000.00 10000.00 0.00 0.02219 21200.00 10000.00 0.00 0.02405 21400.00 10000.00 0.00 0.02603 21600.00 10000.00 0.00 0.02810 21800.00 10000.00 0.00 0.02972 22000.00 10000.00 0.00 0.03070 22200.00 10000.00 0.00 0.03113 22400.00 10000.00 0.00 0.03149 22600.00 10000.00 0.00 0.03177 22400.00 10200.00 0.00 0.03181 22600.00 10200.00 0.00 0.03209 22400.00 10400.00 0.00 0.03239 22600.00 10400.00 0.00 0.03267 22400.00 10600.00 0.00 0.03302 22600.00 10600.00 0.00 0.03326 22400.00 10800.00 0.00 0.03361 22600.00 10800.00 0.00 0.03380 22200.00 11000.00 0.00 0.03394 22400.00 11000.00 0.00 0.03429 22600.00 11000.00 0.00 0.03444 22200.00 11200.00 0.00 0.03454 22400.00 11200.00 0.00 0.03485 22600.00 11200.00 0.00 0.03498 22200.00 11400.00 0.00 0.03526 22400.00 11400.00 0.00 0.03555 22600.00 11400.00 0.00 0.03568 22200.00 11600.00 0.00 0.03601 22400.00 11600.00 0.00 0.03627 22600.00 11600.00 0.00 0.03641 22200.00 11800.00 0.00 0.03671 22400.00 11800.00 0.00 0.03695 22600.00 11800.00 0.00 0.03711 22000.00 12000.00 0.00 0.03712 22200.00 12000.00 0.00 0.03747 22400.00 12000.00 0.00 0.03776 22600.00 12000.00 0.00 0.03795 22200.00 12200.00 0.00 0.03835 22400.00 12200.00 0.00 0.03870 22600.00 12200.00 0.00 0.03891 22000.00 12400.00 0.00 0.03894 22200.00 12400.00 0.00 0.03939 22400.00 12400.00 0.00 0.03975 22600.00 12400.00 0.00 0.03994 22200.00 12600.00 0.00 0.04039 22400.00 12600.00 0.00 0.04073 22600.00 12600.00 0.00 0.04089 22000.00 12800.00 0.00 0.04098 22200.00 12800.00 0.00 0.04148 22400.00 12800.00 0.00 0.04178 22600.00 12800.00 0.00 0.04192 22000.00 13000.00 0.00 0.04210 22200.00 13000.00 0.00 0.04255 22400.00 13000.00 0.00 0.04280 22600.00 13000.00 0.00 0.04293 22000.00 13200.00 0.00 0.04302 22200.00 13200.00 0.00 0.04341 22400.00 13200.00 0.00 0.04363 22600.00 13200.00 0.00 0.04377 22000.00 13400.00 0.00 0.04393 22200.00 13400.00 0.00 0.04428 22400.00 13400.00 0.00 0.04450 22600.00 13400.00 0.00 0.04464 22000.00 13600.00 0.00 0.04497 22200.00 13600.00 0.00 0.04529 22400.00 13600.00 0.00 0.04551 22600.00 13600.00 0.00 0.04559 22000.00 13800.00 0.00 0.04594 22200.00 13800.00 0.00 0.04622 22400.00 13800.00 0.00 0.04640 22600.00 13800.00 0.00 0.04643 22000.00 14000.00 0.00 0.04688 22200.00 14000.00 0.00 0.04713 22400.00 14000.00 0.00 0.04727 22000.00 14200.00 0.00 0.04793 22200.00 14200.00 0.00 0.04813 22400.00 14200.00 0.00 0.04827 22000.00 14400.00 0.00 0.04889 22200.00 14400.00 0.00 0.04911 22400.00 14400.00 0.00 0.04927 22600.00 14400.00 0.00 0.04928 22000.00 14600.00 0.00 0.04994 22200.00 14600.00 0.00 0.05019 22400.00 14600.00 0.00 0.05038 22600.00 14600.00 0.00 0.05040 22000.00 14800.00 0.00 0.05107 22200.00 14800.00 0.00 0.05137 22400.00 14800.00 0.00 0.05159 22600.00 14800.00 0.00 0.05162 22000.00 15000.00 0.00 0.05233 22200.00 15000.00 0.00 0.05269 22400.00 15000.00 0.00 0.05295 22600.00 15000.00 0.00 0.05300 22000.00 15200.00 0.00 0.05379 22200.00 15200.00 0.00 0.05419 22400.00 15200.00 0.00 0.05447 22600.00 15200.00 0.00 0.05447 22000.00 15400.00 0.00 0.05542 22200.00 15400.00 0.00 0.05581 22400.00 15400.00 0.00 0.05605 22000.00 15600.00 0.00 0.05706 22200.00 15600.00 0.00 0.05741 22400.00 15600.00 0.00 0.05760 22000.00 15800.00 0.00 0.05850 22200.00 15800.00 0.00 0.05881 22400.00 15800.00 0.00 0.05893 22000.00 16000.00 0.00 0.05990 22200.00 16000.00 0.00 0.06014 22400.00 16000.00 0.00 0.06017 21800.00 16200.00 0.00 0.06026 22000.00 16200.00 0.00 0.06127 22200.00 16200.00 0.00 0.06144 21800.00 16400.00 0.00 0.06174 22000.00 16400.00 0.00 0.06271 22200.00 16400.00 0.00 0.06282 21800.00 16600.00 0.00 0.06342 22000.00 16600.00 0.00 0.06435 22200.00 16600.00 0.00 0.06445 21800.00 16800.00 0.00 0.06524 22000.00 16800.00 0.00 0.06614 22200.00 16800.00 0.00 0.06624 21800.00 17000.00 0.00 0.06697 22000.00 17000.00 0.00 0.06785 22200.00 17000.00 0.00 0.06793 21800.00 17200.00 0.00 0.06875 22000.00 17200.00 0.00 0.06961 22200.00 17200.00 0.00 0.06967 21800.00 17400.00 0.00 0.07068 22000.00 17400.00 0.00 0.07151 22200.00 17400.00 0.00 0.07154 21800.00 17600.00 0.00 0.07256 22000.00 17600.00 0.00 0.07336 21800.00 17800.00 0.00 0.07460 22000.00 17800.00 0.00 0.07537 21800.00 18000.00 0.00 0.07660 22000.00 18000.00 0.00 0.07730 21800.00 18200.00 0.00 0.07854 22000.00 18200.00 0.00 0.07919 21800.00 18400.00 0.00 0.08041 22000.00 18400.00 0.00 0.08101 21800.00 18600.00 0.00 0.08227 22000.00 18600.00 0.00 0.08283 21800.00 18800.00 0.00 0.08419 22000.00 18800.00 0.00 0.08472 21800.00 19000.00 0.00 0.08614 22000.00 19000.00 0.00 0.08667 21800.00 19200.00 0.00 0.08848 22000.00 19200.00 0.00 0.08896 21800.00 19400.00 0.00 0.09083 22000.00 19400.00 0.00 0.09123 21600.00 19600.00 0.00 0.09137 21800.00 19600.00 0.00 0.09326 22000.00 19600.00 0.00 0.09357 21600.00 19800.00 0.00 0.09378 21800.00 19800.00 0.00 0.09562 22000.00 19800.00 0.00 0.09581 21600.00 20000.00 0.00 0.09623 21800.00 20000.00 0.00 0.09801 22000.00 20000.00 0.00 0.09804 21600.00 20200.00 0.00 0.09886 21800.00 20200.00 0.00 0.10052 21600.00 20400.00 0.00 0.10140 21800.00 20400.00 0.00 0.10292 21600.00 20600.00 0.00 0.10411 21800.00 20600.00 0.00 0.10543 21600.00 20800.00 0.00 0.10648 21800.00 20800.00 0.00 0.10763 21600.00 21000.00 0.00 0.10902 21800.00 21000.00 0.00 0.10996 21600.00 21200.00 0.00 0.11146 21800.00 21200.00 0.00 0.11215 21600.00 21400.00 0.00 0.11368 21800.00 21400.00 0.00 0.11394 21600.00 21600.00 0.00 0.11574 21600.00 21800.00 0.00 0.11614 21600.00 21800.00 5.00 0.11625 21600.00 21800.00 10.00 0.11635 21600.00 21800.00 15.00 0.11643 21600.00 21800.00 20.00 0.11648 21600.00 21800.00 25.00 0.11651 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21585.27 21800.00 27.63 0.11652 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/529.ctf NX, NY, NZ: 512 512 1 MODE : real