CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/528.mrc Output diagnostic file name Micrographs_0601/528.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/528.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2202.000 8548.000 Mean, RMS : 4512.834 227.7367 TITLE 1: emi2mrc: 528.emi Mon Jan 06 17:10:04 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 35.111, Y 486.940, Z -32.881, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01466 10200.00 10000.00 0.00 -0.01453 10400.00 10000.00 0.00 -0.01440 10600.00 10000.00 0.00 -0.01425 10800.00 10000.00 0.00 -0.01397 11000.00 10000.00 0.00 -0.01375 11200.00 10000.00 0.00 -0.01337 11400.00 10000.00 0.00 -0.01310 11600.00 10000.00 0.00 -0.01298 11800.00 10000.00 0.00 -0.01271 12000.00 10000.00 0.00 -0.01245 12200.00 10000.00 0.00 -0.01219 12400.00 10000.00 0.00 -0.01187 12600.00 10000.00 0.00 -0.01141 12800.00 10000.00 0.00 -0.01101 13000.00 10000.00 0.00 -0.01082 13200.00 10000.00 0.00 -0.01054 13400.00 10000.00 0.00 -0.01008 13600.00 10000.00 0.00 -0.00966 13800.00 10000.00 0.00 -0.00935 14000.00 10000.00 0.00 -0.00895 14200.00 10000.00 0.00 -0.00836 14400.00 10000.00 0.00 -0.00752 14600.00 10000.00 0.00 -0.00661 14800.00 10000.00 0.00 -0.00572 15000.00 10000.00 0.00 -0.00498 15200.00 10000.00 0.00 -0.00424 15400.00 10000.00 0.00 -0.00325 15600.00 10000.00 0.00 -0.00224 15800.00 10000.00 0.00 -0.00120 16000.00 10000.00 0.00 -0.00021 16200.00 10000.00 0.00 0.00062 16400.00 10000.00 0.00 0.00156 16600.00 10000.00 0.00 0.00269 16800.00 10000.00 0.00 0.00388 17000.00 10000.00 0.00 0.00508 17200.00 10000.00 0.00 0.00634 17400.00 10000.00 0.00 0.00753 17600.00 10000.00 0.00 0.00858 17800.00 10000.00 0.00 0.00972 18000.00 10000.00 0.00 0.01102 18200.00 10000.00 0.00 0.01235 18400.00 10000.00 0.00 0.01356 18600.00 10000.00 0.00 0.01477 18800.00 10000.00 0.00 0.01608 19000.00 10000.00 0.00 0.01743 19200.00 10000.00 0.00 0.01889 19400.00 10000.00 0.00 0.02043 19600.00 10000.00 0.00 0.02200 19800.00 10000.00 0.00 0.02358 20000.00 10000.00 0.00 0.02516 20200.00 10000.00 0.00 0.02713 20400.00 10000.00 0.00 0.02929 20600.00 10000.00 0.00 0.03131 20800.00 10000.00 0.00 0.03361 21000.00 10000.00 0.00 0.03591 21200.00 10000.00 0.00 0.03797 21400.00 10000.00 0.00 0.03977 21600.00 10000.00 0.00 0.04093 21800.00 10000.00 0.00 0.04149 22000.00 10000.00 0.00 0.04178 22200.00 10000.00 0.00 0.04189 21800.00 10200.00 0.00 0.04217 22000.00 10200.00 0.00 0.04248 22200.00 10200.00 0.00 0.04257 21800.00 10400.00 0.00 0.04298 22000.00 10400.00 0.00 0.04328 22200.00 10400.00 0.00 0.04332 21800.00 10600.00 0.00 0.04379 22000.00 10600.00 0.00 0.04406 21800.00 10800.00 0.00 0.04446 22000.00 10800.00 0.00 0.04467 21800.00 11000.00 0.00 0.04506 22000.00 11000.00 0.00 0.04524 21800.00 11200.00 0.00 0.04564 22000.00 11200.00 0.00 0.04582 21800.00 11400.00 0.00 0.04631 22000.00 11400.00 0.00 0.04648 21800.00 11600.00 0.00 0.04706 22000.00 11600.00 0.00 0.04724 21600.00 11800.00 0.00 0.04732 21800.00 11800.00 0.00 0.04793 22000.00 11800.00 0.00 0.04808 21600.00 12000.00 0.00 0.04820 21800.00 12000.00 0.00 0.04877 22000.00 12000.00 0.00 0.04887 21600.00 12200.00 0.00 0.04900 21800.00 12200.00 0.00 0.04955 22000.00 12200.00 0.00 0.04961 21600.00 12400.00 0.00 0.04983 21800.00 12400.00 0.00 0.05038 22000.00 12400.00 0.00 0.05041 21600.00 12600.00 0.00 0.05070 21800.00 12600.00 0.00 0.05119 21600.00 12800.00 0.00 0.05153 21800.00 12800.00 0.00 0.05194 21600.00 13000.00 0.00 0.05223 21800.00 13000.00 0.00 0.05262 21600.00 13200.00 0.00 0.05301 21800.00 13200.00 0.00 0.05342 21600.00 13400.00 0.00 0.05384 21800.00 13400.00 0.00 0.05426 21600.00 13600.00 0.00 0.05476 21800.00 13600.00 0.00 0.05516 22000.00 13600.00 0.00 0.05516 21600.00 13800.00 0.00 0.05577 21800.00 13800.00 0.00 0.05618 22000.00 13800.00 0.00 0.05623 22200.00 13800.00 0.00 0.05623 21600.00 14000.00 0.00 0.05685 21800.00 14000.00 0.00 0.05731 22000.00 14000.00 0.00 0.05741 22200.00 14000.00 0.00 0.05742 21600.00 14200.00 0.00 0.05815 21800.00 14200.00 0.00 0.05863 22000.00 14200.00 0.00 0.05873 21600.00 14400.00 0.00 0.05945 21800.00 14400.00 0.00 0.05988 22000.00 14400.00 0.00 0.05993 21600.00 14600.00 0.00 0.06064 21800.00 14600.00 0.00 0.06100 22000.00 14600.00 0.00 0.06104 21600.00 14800.00 0.00 0.06173 21800.00 14800.00 0.00 0.06212 22000.00 14800.00 0.00 0.06221 21600.00 15000.00 0.00 0.06292 21800.00 15000.00 0.00 0.06336 22000.00 15000.00 0.00 0.06346 21600.00 15200.00 0.00 0.06416 21800.00 15200.00 0.00 0.06462 22000.00 15200.00 0.00 0.06470 21600.00 15400.00 0.00 0.06558 21800.00 15400.00 0.00 0.06607 22000.00 15400.00 0.00 0.06616 21600.00 15600.00 0.00 0.06720 21800.00 15600.00 0.00 0.06768 22000.00 15600.00 0.00 0.06776 21600.00 15800.00 0.00 0.06871 21800.00 15800.00 0.00 0.06914 22000.00 15800.00 0.00 0.06916 21600.00 16000.00 0.00 0.07027 21800.00 16000.00 0.00 0.07063 21600.00 16200.00 0.00 0.07154 21800.00 16200.00 0.00 0.07185 21600.00 16400.00 0.00 0.07279 21800.00 16400.00 0.00 0.07307 21400.00 16600.00 0.00 0.07311 21600.00 16600.00 0.00 0.07428 21800.00 16600.00 0.00 0.07460 21400.00 16800.00 0.00 0.07465 21600.00 16800.00 0.00 0.07582 21800.00 16800.00 0.00 0.07616 21400.00 17000.00 0.00 0.07634 21600.00 17000.00 0.00 0.07752 21800.00 17000.00 0.00 0.07786 21400.00 17200.00 0.00 0.07810 21600.00 17200.00 0.00 0.07931 21800.00 17200.00 0.00 0.07964 21400.00 17400.00 0.00 0.07992 21600.00 17400.00 0.00 0.08117 21800.00 17400.00 0.00 0.08149 21400.00 17600.00 0.00 0.08179 21600.00 17600.00 0.00 0.08304 21800.00 17600.00 0.00 0.08328 21400.00 17800.00 0.00 0.08367 21600.00 17800.00 0.00 0.08485 21800.00 17800.00 0.00 0.08498 21400.00 18000.00 0.00 0.08549 21600.00 18000.00 0.00 0.08662 21800.00 18000.00 0.00 0.08667 21400.00 18200.00 0.00 0.08736 21600.00 18200.00 0.00 0.08847 21800.00 18200.00 0.00 0.08848 21400.00 18400.00 0.00 0.08925 21600.00 18400.00 0.00 0.09036 21400.00 18600.00 0.00 0.09130 21600.00 18600.00 0.00 0.09240 21400.00 18800.00 0.00 0.09341 21600.00 18800.00 0.00 0.09449 21400.00 19000.00 0.00 0.09551 21600.00 19000.00 0.00 0.09660 21400.00 19200.00 0.00 0.09794 21600.00 19200.00 0.00 0.09901 21400.00 19400.00 0.00 0.10032 21600.00 19400.00 0.00 0.10137 21400.00 19600.00 0.00 0.10288 21600.00 19600.00 0.00 0.10385 21400.00 19800.00 0.00 0.10562 21600.00 19800.00 0.00 0.10648 21400.00 20000.00 0.00 0.10850 21600.00 20000.00 0.00 0.10924 21400.00 20200.00 0.00 0.11146 21600.00 20200.00 0.00 0.11205 21200.00 20400.00 0.00 0.11217 21400.00 20400.00 0.00 0.11435 21600.00 20400.00 0.00 0.11472 21200.00 20600.00 0.00 0.11558 21400.00 20600.00 0.00 0.11759 21600.00 20600.00 0.00 0.11771 21200.00 20800.00 0.00 0.11891 21400.00 20800.00 0.00 0.12075 21200.00 21000.00 0.00 0.12247 21400.00 21000.00 0.00 0.12397 21200.00 21200.00 0.00 0.12541 21400.00 21200.00 0.00 0.12633 21200.00 21400.00 0.00 0.12654 21400.00 21400.00 0.00 0.12689 21200.00 21400.00 40.00 0.12689 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21297.88 21423.58 37.12 0.12716 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/528.ctf NX, NY, NZ: 512 512 1 MODE : real