CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/526.mrc Output diagnostic file name Micrographs_0601/526.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/526.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2360.000 9238.000 Mean, RMS : 4592.877 221.1438 TITLE 1: emi2mrc: 526.emi Mon Jan 06 17:07:09 2014 TITLE 2: kV 300, Mag 78000x, Def 20020, Spot 4, Int 55.04%, Obj 88.05%, Dif 73.67% TITLE 3: X 28.681, Y 495.044, Z -31.147, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01325 10600.00 10000.00 0.00 -0.01320 10800.00 10000.00 0.00 -0.01304 11000.00 10000.00 0.00 -0.01285 11200.00 10000.00 0.00 -0.01267 11400.00 10000.00 0.00 -0.01238 11600.00 10000.00 0.00 -0.01197 11800.00 10000.00 0.00 -0.01150 12000.00 10000.00 0.00 -0.01098 12200.00 10000.00 0.00 -0.01041 12400.00 10000.00 0.00 -0.00993 12600.00 10000.00 0.00 -0.00927 12800.00 10000.00 0.00 -0.00850 13000.00 10000.00 0.00 -0.00798 13200.00 10000.00 0.00 -0.00760 13400.00 10000.00 0.00 -0.00707 13600.00 10000.00 0.00 -0.00636 13800.00 10000.00 0.00 -0.00575 14000.00 10000.00 0.00 -0.00520 14200.00 10000.00 0.00 -0.00466 14400.00 10000.00 0.00 -0.00400 14600.00 10000.00 0.00 -0.00313 14800.00 10000.00 0.00 -0.00213 15000.00 10000.00 0.00 -0.00109 15200.00 10000.00 0.00 -0.00020 15400.00 10000.00 0.00 0.00059 15600.00 10000.00 0.00 0.00155 15800.00 10000.00 0.00 0.00281 16000.00 10000.00 0.00 0.00419 16200.00 10000.00 0.00 0.00552 16400.00 10000.00 0.00 0.00682 16600.00 10000.00 0.00 0.00805 16800.00 10000.00 0.00 0.00933 17000.00 10000.00 0.00 0.01075 17200.00 10000.00 0.00 0.01225 17400.00 10000.00 0.00 0.01377 17600.00 10000.00 0.00 0.01520 17800.00 10000.00 0.00 0.01649 18000.00 10000.00 0.00 0.01777 18200.00 10000.00 0.00 0.01916 18400.00 10000.00 0.00 0.02064 18600.00 10000.00 0.00 0.02224 18800.00 10000.00 0.00 0.02410 19000.00 10000.00 0.00 0.02607 19200.00 10000.00 0.00 0.02818 19400.00 10000.00 0.00 0.03041 19600.00 10000.00 0.00 0.03250 19800.00 10000.00 0.00 0.03456 20000.00 10000.00 0.00 0.03670 20200.00 10000.00 0.00 0.03869 20400.00 10000.00 0.00 0.04020 20600.00 10000.00 0.00 0.04123 20800.00 10000.00 0.00 0.04189 21000.00 10000.00 0.00 0.04222 21200.00 10000.00 0.00 0.04237 21400.00 10000.00 0.00 0.04247 21600.00 10000.00 0.00 0.04254 20800.00 10200.00 0.00 0.04274 21000.00 10200.00 0.00 0.04307 21200.00 10200.00 0.00 0.04322 21400.00 10200.00 0.00 0.04334 21600.00 10200.00 0.00 0.04339 20800.00 10400.00 0.00 0.04360 21000.00 10400.00 0.00 0.04392 21200.00 10400.00 0.00 0.04406 21400.00 10400.00 0.00 0.04416 21600.00 10400.00 0.00 0.04416 20800.00 10600.00 0.00 0.04442 21000.00 10600.00 0.00 0.04472 21200.00 10600.00 0.00 0.04484 21400.00 10600.00 0.00 0.04492 20800.00 10800.00 0.00 0.04525 21000.00 10800.00 0.00 0.04553 21200.00 10800.00 0.00 0.04566 21400.00 10800.00 0.00 0.04574 21600.00 10800.00 0.00 0.04574 20800.00 11000.00 0.00 0.04616 21000.00 11000.00 0.00 0.04645 21200.00 11000.00 0.00 0.04660 21400.00 11000.00 0.00 0.04670 21600.00 11000.00 0.00 0.04670 20800.00 11200.00 0.00 0.04712 21000.00 11200.00 0.00 0.04742 21200.00 11200.00 0.00 0.04758 21400.00 11200.00 0.00 0.04766 20800.00 11400.00 0.00 0.04809 21000.00 11400.00 0.00 0.04839 21200.00 11400.00 0.00 0.04854 21400.00 11400.00 0.00 0.04858 20800.00 11600.00 0.00 0.04893 21000.00 11600.00 0.00 0.04926 21200.00 11600.00 0.00 0.04941 21400.00 11600.00 0.00 0.04943 20800.00 11800.00 0.00 0.04988 21000.00 11800.00 0.00 0.05023 21200.00 11800.00 0.00 0.05035 20800.00 12000.00 0.00 0.05083 21000.00 12000.00 0.00 0.05115 21200.00 12000.00 0.00 0.05123 20800.00 12200.00 0.00 0.05176 21000.00 12200.00 0.00 0.05207 21200.00 12200.00 0.00 0.05215 20800.00 12400.00 0.00 0.05280 21000.00 12400.00 0.00 0.05312 21200.00 12400.00 0.00 0.05320 20800.00 12600.00 0.00 0.05380 21000.00 12600.00 0.00 0.05412 21200.00 12600.00 0.00 0.05416 20600.00 12800.00 0.00 0.05422 20800.00 12800.00 0.00 0.05493 21000.00 12800.00 0.00 0.05521 20600.00 13000.00 0.00 0.05530 20800.00 13000.00 0.00 0.05599 21000.00 13000.00 0.00 0.05621 20600.00 13200.00 0.00 0.05637 20800.00 13200.00 0.00 0.05704 21000.00 13200.00 0.00 0.05723 20600.00 13400.00 0.00 0.05760 20800.00 13400.00 0.00 0.05828 21000.00 13400.00 0.00 0.05847 20600.00 13600.00 0.00 0.05915 20800.00 13600.00 0.00 0.05982 21000.00 13600.00 0.00 0.06000 20600.00 13800.00 0.00 0.06067 20800.00 13800.00 0.00 0.06134 21000.00 13800.00 0.00 0.06152 20600.00 14000.00 0.00 0.06218 20800.00 14000.00 0.00 0.06285 21000.00 14000.00 0.00 0.06300 20600.00 14200.00 0.00 0.06387 20800.00 14200.00 0.00 0.06448 21000.00 14200.00 0.00 0.06456 20600.00 14400.00 0.00 0.06533 20800.00 14400.00 0.00 0.06587 21000.00 14400.00 0.00 0.06590 20600.00 14600.00 0.00 0.06682 20800.00 14600.00 0.00 0.06733 21000.00 14600.00 0.00 0.06736 20600.00 14800.00 0.00 0.06821 20800.00 14800.00 0.00 0.06872 21000.00 14800.00 0.00 0.06875 20600.00 15000.00 0.00 0.06959 20800.00 15000.00 0.00 0.07011 21000.00 15000.00 0.00 0.07014 20600.00 15200.00 0.00 0.07112 20800.00 15200.00 0.00 0.07163 21000.00 15200.00 0.00 0.07165 20600.00 15400.00 0.00 0.07265 20800.00 15400.00 0.00 0.07316 20400.00 15600.00 0.00 0.07326 20600.00 15600.00 0.00 0.07433 20800.00 15600.00 0.00 0.07482 20400.00 15800.00 0.00 0.07490 20600.00 15800.00 0.00 0.07599 20800.00 15800.00 0.00 0.07648 20400.00 16000.00 0.00 0.07669 20600.00 16000.00 0.00 0.07780 20800.00 16000.00 0.00 0.07826 20400.00 16200.00 0.00 0.07854 20600.00 16200.00 0.00 0.07965 20800.00 16200.00 0.00 0.08009 20400.00 16400.00 0.00 0.08060 20600.00 16400.00 0.00 0.08170 20800.00 16400.00 0.00 0.08208 20400.00 16600.00 0.00 0.08271 20600.00 16600.00 0.00 0.08375 20800.00 16600.00 0.00 0.08405 20400.00 16800.00 0.00 0.08462 20600.00 16800.00 0.00 0.08563 20800.00 16800.00 0.00 0.08587 20400.00 17000.00 0.00 0.08661 20600.00 17000.00 0.00 0.08759 20800.00 17000.00 0.00 0.08775 20400.00 17200.00 0.00 0.08865 20600.00 17200.00 0.00 0.08955 20800.00 17200.00 0.00 0.08958 20400.00 17400.00 0.00 0.09071 20600.00 17400.00 0.00 0.09151 20400.00 17600.00 0.00 0.09268 20600.00 17600.00 0.00 0.09345 20400.00 17800.00 0.00 0.09494 20600.00 17800.00 0.00 0.09567 20400.00 18000.00 0.00 0.09730 20600.00 18000.00 0.00 0.09797 20200.00 18200.00 0.00 0.09810 20400.00 18200.00 0.00 0.09982 20600.00 18200.00 0.00 0.10040 20200.00 18400.00 0.00 0.10072 20400.00 18400.00 0.00 0.10237 20600.00 18400.00 0.00 0.10283 20200.00 18600.00 0.00 0.10315 20400.00 18600.00 0.00 0.10475 20600.00 18600.00 0.00 0.10511 20200.00 18800.00 0.00 0.10577 20400.00 18800.00 0.00 0.10730 20600.00 18800.00 0.00 0.10754 20200.00 19000.00 0.00 0.10834 20400.00 19000.00 0.00 0.10978 20600.00 19000.00 0.00 0.10983 20200.00 19200.00 0.00 0.11111 20400.00 19200.00 0.00 0.11241 20200.00 19400.00 0.00 0.11384 20400.00 19400.00 0.00 0.11496 20200.00 19600.00 0.00 0.11656 20400.00 19600.00 0.00 0.11741 20200.00 19800.00 0.00 0.11901 20400.00 19800.00 0.00 0.11948 20200.00 20000.00 0.00 0.12115 20200.00 20200.00 0.00 0.12267 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20212.22 20259.48 30.78 0.12280 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/526.ctf NX, NY, NZ: 512 512 1 MODE : real