CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/525.mrc Output diagnostic file name Micrographs_0601/525.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/525.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2168.000 9120.000 Mean, RMS : 4547.515 216.4817 TITLE 1: emi2mrc: 525.emi Mon Jan 06 17:06:15 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 27.029, Y 497.165, Z -31.147, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01452 10200.00 10000.00 0.00 0.01564 10400.00 10000.00 0.00 0.01681 10600.00 10000.00 0.00 0.01817 10800.00 10000.00 0.00 0.01952 11000.00 10000.00 0.00 0.02098 11200.00 10000.00 0.00 0.02240 11400.00 10000.00 0.00 0.02387 11600.00 10000.00 0.00 0.02561 11800.00 10000.00 0.00 0.02736 12000.00 10000.00 0.00 0.02921 12200.00 10000.00 0.00 0.03109 12400.00 10000.00 0.00 0.03318 12600.00 10000.00 0.00 0.03540 12800.00 10000.00 0.00 0.03766 13000.00 10000.00 0.00 0.04013 13200.00 10000.00 0.00 0.04263 13400.00 10000.00 0.00 0.04506 13600.00 10000.00 0.00 0.04749 13800.00 10000.00 0.00 0.05014 14000.00 10000.00 0.00 0.05291 14200.00 10000.00 0.00 0.05589 14400.00 10000.00 0.00 0.05898 14600.00 10000.00 0.00 0.06193 14800.00 10000.00 0.00 0.06502 15000.00 10000.00 0.00 0.06836 15200.00 10000.00 0.00 0.07151 15400.00 10000.00 0.00 0.07360 15600.00 10000.00 0.00 0.07458 15800.00 10000.00 0.00 0.07481 15400.00 10200.00 0.00 0.07554 15600.00 10200.00 0.00 0.07651 15800.00 10200.00 0.00 0.07668 15400.00 10400.00 0.00 0.07760 15600.00 10400.00 0.00 0.07850 15800.00 10400.00 0.00 0.07858 15400.00 10600.00 0.00 0.07969 15600.00 10600.00 0.00 0.08051 15800.00 10600.00 0.00 0.08055 15400.00 10800.00 0.00 0.08166 15600.00 10800.00 0.00 0.08245 15800.00 10800.00 0.00 0.08250 15400.00 11000.00 0.00 0.08375 15600.00 11000.00 0.00 0.08450 15400.00 11200.00 0.00 0.08591 15600.00 11200.00 0.00 0.08660 15400.00 11400.00 0.00 0.08844 15600.00 11400.00 0.00 0.08910 15400.00 11600.00 0.00 0.09110 15600.00 11600.00 0.00 0.09169 15200.00 11800.00 0.00 0.09205 15400.00 11800.00 0.00 0.09396 15600.00 11800.00 0.00 0.09447 15200.00 12000.00 0.00 0.09505 15400.00 12000.00 0.00 0.09690 15600.00 12000.00 0.00 0.09735 15200.00 12200.00 0.00 0.09808 15400.00 12200.00 0.00 0.09986 15600.00 12200.00 0.00 0.10027 15200.00 12400.00 0.00 0.10125 15400.00 12400.00 0.00 0.10295 15600.00 12400.00 0.00 0.10329 15200.00 12600.00 0.00 0.10418 15400.00 12600.00 0.00 0.10587 15600.00 12600.00 0.00 0.10621 15200.00 12800.00 0.00 0.10739 15400.00 12800.00 0.00 0.10911 15600.00 12800.00 0.00 0.10945 15200.00 13000.00 0.00 0.11070 15400.00 13000.00 0.00 0.11237 15600.00 13000.00 0.00 0.11262 15200.00 13200.00 0.00 0.11405 15400.00 13200.00 0.00 0.11561 15600.00 13200.00 0.00 0.11573 15200.00 13400.00 0.00 0.11716 15400.00 13400.00 0.00 0.11866 15600.00 13400.00 0.00 0.11870 15200.00 13600.00 0.00 0.12049 15400.00 13600.00 0.00 0.12192 15200.00 13800.00 0.00 0.12408 15400.00 13800.00 0.00 0.12537 15200.00 14000.00 0.00 0.12750 15400.00 14000.00 0.00 0.12869 15200.00 14200.00 0.00 0.13121 15400.00 14200.00 0.00 0.13225 15200.00 14400.00 0.00 0.13472 15400.00 14400.00 0.00 0.13547 15200.00 14600.00 0.00 0.13846 15400.00 14600.00 0.00 0.13873 15200.00 14800.00 0.00 0.14146 15000.00 15000.00 0.00 0.14149 15200.00 15000.00 0.00 0.14401 15000.00 15200.00 0.00 0.14405 15200.00 15200.00 0.00 0.14574 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15173.80 15241.35 -13.33 0.14581 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/525.ctf NX, NY, NZ: 512 512 1 MODE : real