CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/524.mrc Output diagnostic file name Micrographs_0601/524.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/524.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2550.000 7622.000 Mean, RMS : 4549.119 220.3841 TITLE 1: emi2mrc: 524.emi Mon Jan 06 17:05:34 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 25.392, Y 499.571, Z -31.147, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00448 10200.00 10000.00 0.00 0.00517 10400.00 10000.00 0.00 0.00581 10600.00 10000.00 0.00 0.00665 10800.00 10000.00 0.00 0.00753 11000.00 10000.00 0.00 0.00841 11200.00 10000.00 0.00 0.00937 11400.00 10000.00 0.00 0.01027 11600.00 10000.00 0.00 0.01134 11800.00 10000.00 0.00 0.01255 12000.00 10000.00 0.00 0.01377 12200.00 10000.00 0.00 0.01490 12400.00 10000.00 0.00 0.01590 12600.00 10000.00 0.00 0.01701 12800.00 10000.00 0.00 0.01839 13000.00 10000.00 0.00 0.02007 13200.00 10000.00 0.00 0.02202 13400.00 10000.00 0.00 0.02419 13600.00 10000.00 0.00 0.02643 13800.00 10000.00 0.00 0.02864 14000.00 10000.00 0.00 0.03086 14200.00 10000.00 0.00 0.03322 14400.00 10000.00 0.00 0.03556 14600.00 10000.00 0.00 0.03782 14800.00 10000.00 0.00 0.04002 15000.00 10000.00 0.00 0.04213 15200.00 10000.00 0.00 0.04436 15400.00 10000.00 0.00 0.04679 15600.00 10000.00 0.00 0.04954 15800.00 10000.00 0.00 0.05258 16000.00 10000.00 0.00 0.05584 16200.00 10000.00 0.00 0.05954 16400.00 10000.00 0.00 0.06317 16600.00 10000.00 0.00 0.06541 16800.00 10000.00 0.00 0.06615 17000.00 10000.00 0.00 0.06646 16600.00 10200.00 0.00 0.06678 16800.00 10200.00 0.00 0.06751 17000.00 10200.00 0.00 0.06784 16600.00 10400.00 0.00 0.06840 16800.00 10400.00 0.00 0.06914 17000.00 10400.00 0.00 0.06946 16600.00 10600.00 0.00 0.07008 16800.00 10600.00 0.00 0.07077 17000.00 10600.00 0.00 0.07103 16600.00 10800.00 0.00 0.07172 16800.00 10800.00 0.00 0.07238 17000.00 10800.00 0.00 0.07261 16600.00 11000.00 0.00 0.07354 16800.00 11000.00 0.00 0.07418 17000.00 11000.00 0.00 0.07440 16600.00 11200.00 0.00 0.07533 16800.00 11200.00 0.00 0.07596 17000.00 11200.00 0.00 0.07614 16600.00 11400.00 0.00 0.07720 16800.00 11400.00 0.00 0.07780 17000.00 11400.00 0.00 0.07794 16600.00 11600.00 0.00 0.07916 16800.00 11600.00 0.00 0.07976 17000.00 11600.00 0.00 0.07990 16600.00 11800.00 0.00 0.08138 16800.00 11800.00 0.00 0.08196 17000.00 11800.00 0.00 0.08207 16600.00 12000.00 0.00 0.08355 16800.00 12000.00 0.00 0.08406 17000.00 12000.00 0.00 0.08410 16600.00 12200.00 0.00 0.08562 16800.00 12200.00 0.00 0.08608 17000.00 12200.00 0.00 0.08612 16600.00 12400.00 0.00 0.08779 16800.00 12400.00 0.00 0.08830 17000.00 12400.00 0.00 0.08834 16600.00 12600.00 0.00 0.09003 16800.00 12600.00 0.00 0.09055 16600.00 12800.00 0.00 0.09249 16800.00 12800.00 0.00 0.09303 16600.00 13000.00 0.00 0.09506 16800.00 13000.00 0.00 0.09555 16400.00 13200.00 0.00 0.09561 16600.00 13200.00 0.00 0.09787 16800.00 13200.00 0.00 0.09825 16400.00 13400.00 0.00 0.09844 16600.00 13400.00 0.00 0.10067 16800.00 13400.00 0.00 0.10097 16400.00 13600.00 0.00 0.10133 16600.00 13600.00 0.00 0.10356 16800.00 13600.00 0.00 0.10379 16400.00 13800.00 0.00 0.10440 16600.00 13800.00 0.00 0.10663 16800.00 13800.00 0.00 0.10675 16400.00 14000.00 0.00 0.10764 16600.00 14000.00 0.00 0.10983 16400.00 14200.00 0.00 0.11117 16600.00 14200.00 0.00 0.11332 16400.00 14400.00 0.00 0.11460 16600.00 14400.00 0.00 0.11672 16400.00 14600.00 0.00 0.11818 16600.00 14600.00 0.00 0.12024 16400.00 14800.00 0.00 0.12173 16600.00 14800.00 0.00 0.12365 16400.00 15000.00 0.00 0.12554 16600.00 15000.00 0.00 0.12729 16400.00 15200.00 0.00 0.12950 16600.00 15200.00 0.00 0.13105 16400.00 15400.00 0.00 0.13363 16600.00 15400.00 0.00 0.13494 16400.00 15600.00 0.00 0.13822 16600.00 15600.00 0.00 0.13919 16400.00 15800.00 0.00 0.14266 16600.00 15800.00 0.00 0.14320 16200.00 16000.00 0.00 0.14353 16400.00 16000.00 0.00 0.14740 16200.00 16200.00 0.00 0.14839 16400.00 16200.00 0.00 0.15178 16200.00 16400.00 0.00 0.15230 16400.00 16400.00 0.00 0.15478 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16391.89 16443.27 8.99 0.15491 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/524.ctf NX, NY, NZ: 512 512 1 MODE : real