CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/522.mrc Output diagnostic file name Micrographs_0601/522.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/522.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2402.000 9734.000 Mean, RMS : 4549.288 221.5137 TITLE 1: emi2mrc: 522.emi Mon Jan 06 17:04:09 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 18.514, Y 504.786, Z -31.147, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02643 10800.00 10000.00 0.00 -0.02641 11000.00 10000.00 0.00 -0.02610 11200.00 10000.00 0.00 -0.02578 11400.00 10000.00 0.00 -0.02565 11800.00 10000.00 0.00 -0.02548 12000.00 10000.00 0.00 -0.02543 12600.00 10000.00 0.00 -0.02536 12800.00 10000.00 0.00 -0.02523 13000.00 10000.00 0.00 -0.02514 13200.00 10000.00 0.00 -0.02501 13400.00 10000.00 0.00 -0.02484 13600.00 10000.00 0.00 -0.02464 13800.00 10000.00 0.00 -0.02438 14000.00 10000.00 0.00 -0.02397 14200.00 10000.00 0.00 -0.02360 14400.00 10000.00 0.00 -0.02323 14600.00 10000.00 0.00 -0.02278 14800.00 10000.00 0.00 -0.02226 15000.00 10000.00 0.00 -0.02155 15200.00 10000.00 0.00 -0.02069 15400.00 10000.00 0.00 -0.01988 15600.00 10000.00 0.00 -0.01908 15800.00 10000.00 0.00 -0.01824 16000.00 10000.00 0.00 -0.01737 16200.00 10000.00 0.00 -0.01646 16400.00 10000.00 0.00 -0.01549 16600.00 10000.00 0.00 -0.01439 16800.00 10000.00 0.00 -0.01340 17000.00 10000.00 0.00 -0.01257 17200.00 10000.00 0.00 -0.01178 17400.00 10000.00 0.00 -0.01094 17600.00 10000.00 0.00 -0.00990 17800.00 10000.00 0.00 -0.00888 18000.00 10000.00 0.00 -0.00786 18200.00 10000.00 0.00 -0.00690 18400.00 10000.00 0.00 -0.00605 18600.00 10000.00 0.00 -0.00495 18800.00 10000.00 0.00 -0.00384 19000.00 10000.00 0.00 -0.00290 19200.00 10000.00 0.00 -0.00196 19400.00 10000.00 0.00 -0.00080 19600.00 10000.00 0.00 0.00055 19800.00 10000.00 0.00 0.00179 20000.00 10000.00 0.00 0.00302 20200.00 10000.00 0.00 0.00432 20400.00 10000.00 0.00 0.00541 20600.00 10000.00 0.00 0.00650 20800.00 10000.00 0.00 0.00763 21000.00 10000.00 0.00 0.00880 21200.00 10000.00 0.00 0.01017 21400.00 10000.00 0.00 0.01162 21600.00 10000.00 0.00 0.01299 21800.00 10000.00 0.00 0.01435 22000.00 10000.00 0.00 0.01581 22200.00 10000.00 0.00 0.01734 22400.00 10000.00 0.00 0.01892 22600.00 10000.00 0.00 0.02049 22800.00 10000.00 0.00 0.02203 23000.00 10000.00 0.00 0.02354 23200.00 10000.00 0.00 0.02500 23400.00 10000.00 0.00 0.02637 23600.00 10000.00 0.00 0.02741 23800.00 10000.00 0.00 0.02810 24000.00 10000.00 0.00 0.02854 24200.00 10000.00 0.00 0.02886 24400.00 10000.00 0.00 0.02903 24200.00 10200.00 0.00 0.02933 24400.00 10200.00 0.00 0.02946 24200.00 10400.00 0.00 0.02974 24400.00 10400.00 0.00 0.02982 24000.00 10600.00 0.00 0.02992 24200.00 10600.00 0.00 0.03021 24400.00 10600.00 0.00 0.03026 24000.00 10800.00 0.00 0.03053 24200.00 10800.00 0.00 0.03078 24400.00 10800.00 0.00 0.03080 24000.00 11000.00 0.00 0.03109 24200.00 11000.00 0.00 0.03128 24400.00 11000.00 0.00 0.03131 24000.00 11200.00 0.00 0.03161 24200.00 11200.00 0.00 0.03178 24400.00 11200.00 0.00 0.03181 24000.00 11400.00 0.00 0.03213 24200.00 11400.00 0.00 0.03231 24400.00 11400.00 0.00 0.03235 24000.00 11600.00 0.00 0.03268 24200.00 11600.00 0.00 0.03286 24400.00 11600.00 0.00 0.03291 23800.00 11800.00 0.00 0.03292 24000.00 11800.00 0.00 0.03329 24200.00 11800.00 0.00 0.03344 24400.00 11800.00 0.00 0.03345 24000.00 12000.00 0.00 0.03377 24200.00 12000.00 0.00 0.03390 23800.00 12200.00 0.00 0.03397 24000.00 12200.00 0.00 0.03430 24200.00 12200.00 0.00 0.03445 23800.00 12400.00 0.00 0.03461 24000.00 12400.00 0.00 0.03493 24200.00 12400.00 0.00 0.03506 23800.00 12600.00 0.00 0.03524 24000.00 12600.00 0.00 0.03552 24200.00 12600.00 0.00 0.03564 23800.00 12800.00 0.00 0.03588 24000.00 12800.00 0.00 0.03617 24200.00 12800.00 0.00 0.03630 23800.00 13000.00 0.00 0.03665 24000.00 13000.00 0.00 0.03695 24200.00 13000.00 0.00 0.03707 23600.00 13200.00 0.00 0.03709 23800.00 13200.00 0.00 0.03763 24000.00 13200.00 0.00 0.03790 24200.00 13200.00 0.00 0.03796 23600.00 13400.00 0.00 0.03799 23800.00 13400.00 0.00 0.03847 24000.00 13400.00 0.00 0.03868 23600.00 13600.00 0.00 0.03876 23800.00 13600.00 0.00 0.03918 24000.00 13600.00 0.00 0.03937 23600.00 13800.00 0.00 0.03951 23800.00 13800.00 0.00 0.03993 24000.00 13800.00 0.00 0.04012 24200.00 13800.00 0.00 0.04014 23600.00 14000.00 0.00 0.04034 23800.00 14000.00 0.00 0.04077 24000.00 14000.00 0.00 0.04098 24200.00 14000.00 0.00 0.04101 23600.00 14200.00 0.00 0.04123 23800.00 14200.00 0.00 0.04166 24000.00 14200.00 0.00 0.04183 23600.00 14400.00 0.00 0.04199 23800.00 14400.00 0.00 0.04239 24000.00 14400.00 0.00 0.04251 23600.00 14600.00 0.00 0.04287 23800.00 14600.00 0.00 0.04327 24000.00 14600.00 0.00 0.04338 24200.00 14600.00 0.00 0.04340 23600.00 14800.00 0.00 0.04381 23800.00 14800.00 0.00 0.04423 24000.00 14800.00 0.00 0.04435 24200.00 14800.00 0.00 0.04439 23600.00 15000.00 0.00 0.04468 23800.00 15000.00 0.00 0.04511 24000.00 15000.00 0.00 0.04521 24200.00 15000.00 0.00 0.04524 23600.00 15200.00 0.00 0.04556 23800.00 15200.00 0.00 0.04600 24000.00 15200.00 0.00 0.04609 24200.00 15200.00 0.00 0.04611 23600.00 15400.00 0.00 0.04651 23800.00 15400.00 0.00 0.04692 24000.00 15400.00 0.00 0.04697 24200.00 15400.00 0.00 0.04699 23600.00 15600.00 0.00 0.04739 23800.00 15600.00 0.00 0.04775 24000.00 15600.00 0.00 0.04777 24200.00 15600.00 0.00 0.04779 23600.00 15800.00 0.00 0.04824 23800.00 15800.00 0.00 0.04858 24000.00 15800.00 0.00 0.04862 24200.00 15800.00 0.00 0.04868 23600.00 16000.00 0.00 0.04938 23800.00 16000.00 0.00 0.04972 24000.00 16000.00 0.00 0.04980 24200.00 16000.00 0.00 0.04989 23600.00 16200.00 0.00 0.05056 23800.00 16200.00 0.00 0.05090 24000.00 16200.00 0.00 0.05103 24200.00 16200.00 0.00 0.05114 23600.00 16400.00 0.00 0.05179 23800.00 16400.00 0.00 0.05215 24000.00 16400.00 0.00 0.05230 24200.00 16400.00 0.00 0.05239 23600.00 16600.00 0.00 0.05305 23800.00 16600.00 0.00 0.05341 24000.00 16600.00 0.00 0.05354 24200.00 16600.00 0.00 0.05359 23600.00 16800.00 0.00 0.05422 23800.00 16800.00 0.00 0.05457 24000.00 16800.00 0.00 0.05470 24200.00 16800.00 0.00 0.05475 23600.00 17000.00 0.00 0.05560 23800.00 17000.00 0.00 0.05596 24000.00 17000.00 0.00 0.05610 24200.00 17000.00 0.00 0.05615 23400.00 17200.00 0.00 0.05622 23600.00 17200.00 0.00 0.05710 23800.00 17200.00 0.00 0.05746 24000.00 17200.00 0.00 0.05759 24200.00 17200.00 0.00 0.05760 23400.00 17400.00 0.00 0.05775 23600.00 17400.00 0.00 0.05864 23800.00 17400.00 0.00 0.05896 24000.00 17400.00 0.00 0.05907 23400.00 17600.00 0.00 0.05925 23600.00 17600.00 0.00 0.06010 23800.00 17600.00 0.00 0.06036 24000.00 17600.00 0.00 0.06041 23400.00 17800.00 0.00 0.06058 23600.00 17800.00 0.00 0.06137 23800.00 17800.00 0.00 0.06157 24000.00 17800.00 0.00 0.06160 23400.00 18000.00 0.00 0.06191 23600.00 18000.00 0.00 0.06266 23800.00 18000.00 0.00 0.06283 24000.00 18000.00 0.00 0.06284 23400.00 18200.00 0.00 0.06324 23600.00 18200.00 0.00 0.06395 23800.00 18200.00 0.00 0.06408 23400.00 18400.00 0.00 0.06449 23600.00 18400.00 0.00 0.06515 23800.00 18400.00 0.00 0.06523 23400.00 18600.00 0.00 0.06589 23600.00 18600.00 0.00 0.06646 23800.00 18600.00 0.00 0.06648 23400.00 18800.00 0.00 0.06721 23600.00 18800.00 0.00 0.06772 23800.00 18800.00 0.00 0.06774 23400.00 19000.00 0.00 0.06849 23600.00 19000.00 0.00 0.06899 23800.00 19000.00 0.00 0.06902 23400.00 19200.00 0.00 0.06990 23600.00 19200.00 0.00 0.07035 23400.00 19400.00 0.00 0.07104 23600.00 19400.00 0.00 0.07146 23400.00 19600.00 0.00 0.07225 23600.00 19600.00 0.00 0.07267 23400.00 19800.00 0.00 0.07356 23600.00 19800.00 0.00 0.07398 23400.00 20000.00 0.00 0.07491 23600.00 20000.00 0.00 0.07531 23400.00 20200.00 0.00 0.07632 23600.00 20200.00 0.00 0.07670 23400.00 20400.00 0.00 0.07760 23600.00 20400.00 0.00 0.07793 23400.00 20600.00 0.00 0.07888 23600.00 20600.00 0.00 0.07916 23400.00 20800.00 0.00 0.08014 23600.00 20800.00 0.00 0.08040 23200.00 21000.00 0.00 0.08060 23400.00 21000.00 0.00 0.08162 23600.00 21000.00 0.00 0.08184 23200.00 21200.00 0.00 0.08211 23400.00 21200.00 0.00 0.08312 23600.00 21200.00 0.00 0.08326 23200.00 21400.00 0.00 0.08370 23400.00 21400.00 0.00 0.08467 23600.00 21400.00 0.00 0.08473 23200.00 21600.00 0.00 0.08527 23400.00 21600.00 0.00 0.08622 23600.00 21600.00 0.00 0.08623 23200.00 21800.00 0.00 0.08661 23400.00 21800.00 0.00 0.08757 23200.00 22000.00 0.00 0.08804 23400.00 22000.00 0.00 0.08901 23200.00 22200.00 0.00 0.08942 23400.00 22200.00 0.00 0.09034 23200.00 22400.00 0.00 0.09078 23400.00 22400.00 0.00 0.09161 23200.00 22600.00 0.00 0.09198 23400.00 22600.00 0.00 0.09269 23200.00 22800.00 0.00 0.09322 23400.00 22800.00 0.00 0.09374 23200.00 23000.00 0.00 0.09443 23400.00 23000.00 0.00 0.09470 23200.00 23200.00 0.00 0.09539 23200.00 23400.00 0.00 0.09560 23200.00 23400.00 5.00 0.09562 23200.00 23400.00 10.00 0.09563 23200.00 23400.00 15.00 0.09563 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23236.68 23315.33 24.53 0.09576 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/522.ctf NX, NY, NZ: 512 512 1 MODE : real