CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/521.mrc Output diagnostic file name Micrographs_0601/521.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/521.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2330.000 8654.000 Mean, RMS : 4550.820 217.0782 TITLE 1: emi2mrc: 521.emi Mon Jan 06 17:03:29 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 20.140, Y 502.383, Z -31.147, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01431 10200.00 10000.00 0.00 -0.01422 10400.00 10000.00 0.00 -0.01406 10600.00 10000.00 0.00 -0.01389 10800.00 10000.00 0.00 -0.01359 11000.00 10000.00 0.00 -0.01334 11200.00 10000.00 0.00 -0.01308 11400.00 10000.00 0.00 -0.01275 11600.00 10000.00 0.00 -0.01249 11800.00 10000.00 0.00 -0.01213 12000.00 10000.00 0.00 -0.01182 12200.00 10000.00 0.00 -0.01158 12400.00 10000.00 0.00 -0.01118 12600.00 10000.00 0.00 -0.01080 12800.00 10000.00 0.00 -0.01034 13000.00 10000.00 0.00 -0.00969 13200.00 10000.00 0.00 -0.00917 13400.00 10000.00 0.00 -0.00861 13600.00 10000.00 0.00 -0.00784 13800.00 10000.00 0.00 -0.00706 14000.00 10000.00 0.00 -0.00631 14200.00 10000.00 0.00 -0.00555 14400.00 10000.00 0.00 -0.00472 14600.00 10000.00 0.00 -0.00384 14800.00 10000.00 0.00 -0.00286 15000.00 10000.00 0.00 -0.00181 15200.00 10000.00 0.00 -0.00077 15400.00 10000.00 0.00 0.00038 15600.00 10000.00 0.00 0.00153 15800.00 10000.00 0.00 0.00254 16000.00 10000.00 0.00 0.00347 16200.00 10000.00 0.00 0.00449 16400.00 10000.00 0.00 0.00567 16600.00 10000.00 0.00 0.00695 16800.00 10000.00 0.00 0.00816 17000.00 10000.00 0.00 0.00932 17200.00 10000.00 0.00 0.01033 17400.00 10000.00 0.00 0.01129 17600.00 10000.00 0.00 0.01237 17800.00 10000.00 0.00 0.01350 18000.00 10000.00 0.00 0.01488 18200.00 10000.00 0.00 0.01643 18400.00 10000.00 0.00 0.01777 18600.00 10000.00 0.00 0.01906 18800.00 10000.00 0.00 0.02051 19000.00 10000.00 0.00 0.02206 19200.00 10000.00 0.00 0.02359 19400.00 10000.00 0.00 0.02506 19600.00 10000.00 0.00 0.02656 19800.00 10000.00 0.00 0.02816 20000.00 10000.00 0.00 0.02988 20200.00 10000.00 0.00 0.03163 20400.00 10000.00 0.00 0.03340 20600.00 10000.00 0.00 0.03528 20800.00 10000.00 0.00 0.03699 21000.00 10000.00 0.00 0.03851 21200.00 10000.00 0.00 0.03973 21400.00 10000.00 0.00 0.04044 21600.00 10000.00 0.00 0.04085 21800.00 10000.00 0.00 0.04102 22000.00 10000.00 0.00 0.04111 22200.00 10000.00 0.00 0.04123 21600.00 10200.00 0.00 0.04138 21800.00 10200.00 0.00 0.04153 22000.00 10200.00 0.00 0.04159 22200.00 10200.00 0.00 0.04169 21600.00 10400.00 0.00 0.04193 21800.00 10400.00 0.00 0.04205 22000.00 10400.00 0.00 0.04208 22200.00 10400.00 0.00 0.04217 21600.00 10600.00 0.00 0.04250 21800.00 10600.00 0.00 0.04256 22000.00 10600.00 0.00 0.04256 22200.00 10600.00 0.00 0.04265 21600.00 10800.00 0.00 0.04296 22200.00 10800.00 0.00 0.04298 21400.00 11000.00 0.00 0.04303 21600.00 11000.00 0.00 0.04334 22200.00 11000.00 0.00 0.04337 21600.00 11200.00 0.00 0.04366 21800.00 11200.00 0.00 0.04367 22000.00 11200.00 0.00 0.04370 22200.00 11200.00 0.00 0.04375 21400.00 11400.00 0.00 0.04389 21600.00 11400.00 0.00 0.04424 22000.00 11400.00 0.00 0.04428 22200.00 11400.00 0.00 0.04434 21400.00 11600.00 0.00 0.04469 21600.00 11600.00 0.00 0.04500 22000.00 11600.00 0.00 0.04507 22200.00 11600.00 0.00 0.04521 21400.00 11800.00 0.00 0.04555 21600.00 11800.00 0.00 0.04586 21800.00 11800.00 0.00 0.04590 22000.00 11800.00 0.00 0.04608 22200.00 11800.00 0.00 0.04626 21400.00 12000.00 0.00 0.04658 21600.00 12000.00 0.00 0.04695 21800.00 12000.00 0.00 0.04707 22000.00 12000.00 0.00 0.04729 22200.00 12000.00 0.00 0.04744 21400.00 12200.00 0.00 0.04775 21600.00 12200.00 0.00 0.04815 21800.00 12200.00 0.00 0.04826 22000.00 12200.00 0.00 0.04846 22200.00 12200.00 0.00 0.04853 21400.00 12400.00 0.00 0.04880 21600.00 12400.00 0.00 0.04916 21800.00 12400.00 0.00 0.04924 22000.00 12400.00 0.00 0.04940 22200.00 12400.00 0.00 0.04942 21400.00 12600.00 0.00 0.04969 21600.00 12600.00 0.00 0.05004 21800.00 12600.00 0.00 0.05013 22000.00 12600.00 0.00 0.05029 21400.00 12800.00 0.00 0.05067 21600.00 12800.00 0.00 0.05101 21800.00 12800.00 0.00 0.05113 22000.00 12800.00 0.00 0.05129 21400.00 13000.00 0.00 0.05170 21600.00 13000.00 0.00 0.05207 21800.00 13000.00 0.00 0.05219 22000.00 13000.00 0.00 0.05233 21400.00 13200.00 0.00 0.05296 21600.00 13200.00 0.00 0.05335 21800.00 13200.00 0.00 0.05348 22000.00 13200.00 0.00 0.05360 21400.00 13400.00 0.00 0.05427 21600.00 13400.00 0.00 0.05467 21800.00 13400.00 0.00 0.05480 22000.00 13400.00 0.00 0.05491 21400.00 13600.00 0.00 0.05560 21600.00 13600.00 0.00 0.05600 21800.00 13600.00 0.00 0.05613 22000.00 13600.00 0.00 0.05623 21200.00 13800.00 0.00 0.05624 21400.00 13800.00 0.00 0.05700 21600.00 13800.00 0.00 0.05740 21800.00 13800.00 0.00 0.05752 22000.00 13800.00 0.00 0.05760 21200.00 14000.00 0.00 0.05763 21400.00 14000.00 0.00 0.05837 21600.00 14000.00 0.00 0.05876 21800.00 14000.00 0.00 0.05884 22000.00 14000.00 0.00 0.05885 21200.00 14200.00 0.00 0.05902 21400.00 14200.00 0.00 0.05973 21600.00 14200.00 0.00 0.06007 21800.00 14200.00 0.00 0.06010 21200.00 14400.00 0.00 0.06023 21400.00 14400.00 0.00 0.06091 21600.00 14400.00 0.00 0.06122 21200.00 14600.00 0.00 0.06149 21400.00 14600.00 0.00 0.06212 21600.00 14600.00 0.00 0.06239 21200.00 14800.00 0.00 0.06263 21400.00 14800.00 0.00 0.06322 21600.00 14800.00 0.00 0.06346 21200.00 15000.00 0.00 0.06375 21400.00 15000.00 0.00 0.06434 21600.00 15000.00 0.00 0.06459 21200.00 15200.00 0.00 0.06503 21400.00 15200.00 0.00 0.06566 21600.00 15200.00 0.00 0.06591 21200.00 15400.00 0.00 0.06639 21400.00 15400.00 0.00 0.06700 21600.00 15400.00 0.00 0.06718 21200.00 15600.00 0.00 0.06790 21400.00 15600.00 0.00 0.06845 21600.00 15600.00 0.00 0.06856 21200.00 15800.00 0.00 0.06940 21400.00 15800.00 0.00 0.06991 21600.00 15800.00 0.00 0.06999 21200.00 16000.00 0.00 0.07089 21400.00 16000.00 0.00 0.07137 21600.00 16000.00 0.00 0.07144 21200.00 16200.00 0.00 0.07237 21400.00 16200.00 0.00 0.07284 21600.00 16200.00 0.00 0.07291 21200.00 16400.00 0.00 0.07391 21400.00 16400.00 0.00 0.07434 21600.00 16400.00 0.00 0.07440 21200.00 16600.00 0.00 0.07529 21400.00 16600.00 0.00 0.07570 21600.00 16600.00 0.00 0.07576 21200.00 16800.00 0.00 0.07678 21400.00 16800.00 0.00 0.07720 21600.00 16800.00 0.00 0.07727 21000.00 17000.00 0.00 0.07731 21200.00 17000.00 0.00 0.07846 21400.00 17000.00 0.00 0.07886 21600.00 17000.00 0.00 0.07890 21000.00 17200.00 0.00 0.07897 21200.00 17200.00 0.00 0.08009 21400.00 17200.00 0.00 0.08044 21600.00 17200.00 0.00 0.08045 21000.00 17400.00 0.00 0.08064 21200.00 17400.00 0.00 0.08172 21400.00 17400.00 0.00 0.08204 21000.00 17600.00 0.00 0.08216 21200.00 17600.00 0.00 0.08320 21400.00 17600.00 0.00 0.08346 21000.00 17800.00 0.00 0.08379 21200.00 17800.00 0.00 0.08476 21400.00 17800.00 0.00 0.08500 21000.00 18000.00 0.00 0.08530 21200.00 18000.00 0.00 0.08625 21400.00 18000.00 0.00 0.08645 21000.00 18200.00 0.00 0.08683 21200.00 18200.00 0.00 0.08776 21400.00 18200.00 0.00 0.08792 21000.00 18400.00 0.00 0.08858 21200.00 18400.00 0.00 0.08952 21400.00 18400.00 0.00 0.08966 21000.00 18600.00 0.00 0.09042 21200.00 18600.00 0.00 0.09133 21400.00 18600.00 0.00 0.09142 21000.00 18800.00 0.00 0.09241 21200.00 18800.00 0.00 0.09325 21400.00 18800.00 0.00 0.09327 21000.00 19000.00 0.00 0.09429 21200.00 19000.00 0.00 0.09504 21000.00 19200.00 0.00 0.09615 21200.00 19200.00 0.00 0.09683 21000.00 19400.00 0.00 0.09794 21200.00 19400.00 0.00 0.09856 21000.00 19600.00 0.00 0.09982 21200.00 19600.00 0.00 0.10039 20800.00 19800.00 0.00 0.10044 21000.00 19800.00 0.00 0.10167 21200.00 19800.00 0.00 0.10214 20800.00 20000.00 0.00 0.10231 21000.00 20000.00 0.00 0.10345 21200.00 20000.00 0.00 0.10382 20800.00 20200.00 0.00 0.10417 21000.00 20200.00 0.00 0.10523 21200.00 20200.00 0.00 0.10551 20800.00 20400.00 0.00 0.10580 21000.00 20400.00 0.00 0.10681 21200.00 20400.00 0.00 0.10696 20800.00 20600.00 0.00 0.10754 21000.00 20600.00 0.00 0.10840 20800.00 20800.00 0.00 0.10866 21000.00 20800.00 0.00 0.10938 21000.00 21000.00 0.00 0.10983 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20981.07 21008.72 -20.65 0.10984 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/521.ctf NX, NY, NZ: 512 512 1 MODE : real